| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 2,398,822 | T→G | 6.0% | V603V (GTA→GTC) | uvrD ← | DNA‑dependent ATPase I and helicase II |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 2,398,822 | 0 | T | G | 6.0% | 186.3 / 6.1 | 67 | V603V (GTA→GTC) | uvrD | DNA‑dependent ATPase I and helicase II |
| Reads supporting (aligned to +/- strand): ref base T (35/28); new base G (2/2); total (37/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-01 | |||||||||||
GTAAACCTCTTTACCATACAGACGGCGGGTTTCCGCGTAGGTCAGCGTCAGTTTCTGCATCGCGCGGGTTACGCCAACGTAGGCCAGACGGCGTTCTTCTTCCAGACGCCCGCCTTCATCCAGCGACATCTGGCTTGGG > Exported/2398753‑2398891 | gTAAACCTCTTTACCATACAGACGGCGGGTTTCCGCGTAGGTCAGCGTCAGTTTCTGCATCGCGCGGGTTa > 1:4828004/1‑71 (MQ=255)gTAAACCTCTTTACCATACAGACGGCGGGTTTCCGCGTAGGTCAGCGTCAGTTTCTGCATCGCGCGGGTTa > 1:814379/1‑71 (MQ=255)gTAAACCTCTTTACCATACAGACGGCGGGTTTCCGCGTAGGTCAGCGTCAGTTTCTGCATCGCGCGGGTTa > 1:6514358/1‑71 (MQ=255)gTAAACCTCTTTACCATACAGACGGCGGGTTTCCGCGTAGGTCAGCGTCAGTTTCTGCATCGCGCGGGTTa > 1:2658108/1‑71 (MQ=255) tctTTACCATACAGACGGCGGGTTTCCGCGTAGGTCAGCGTCAGTTTCTGCATCGCGCGGGTTACg > 1:6724266/1‑66 (MQ=255) tctTTACCATACAGACGGCGGGTTTCCGCGTAGGTCAGCGTCAGTTTCTGCATCGCGCGGGTTACGCCAAc > 1:6716478/1‑71 (MQ=255) tctTTACCATACAGACGGCGGGTTTCCGCGTAGGTCAGCGTCAGTTTCTGCATCGCGCGGGTTACGCCAAc > 1:6620300/1‑71 (MQ=255) tctTTACCATACAGACGGCGGGTTTCCGCGTAGGTCAGCGTCAGTTTCTGCATCGCGCGGGTTACGCCAAc > 1:2655358/1‑71 (MQ=255) tACCATACAGACGGCGGGTTTCCGCGTAGGTCAGGGTCAGTTTCTGCATCGCGCGGGTTACGCCAACGTa < 1:312851/70‑1 (MQ=255) tACCATACAGACGGCGGGTTTCCGCGTAGGTCAGCGTCAGTTTCTGCATCGCGCGGGTTACGCCAACg < 1:2997834/68‑1 (MQ=255) tACAGACGGCGGGTTTCCGCGTAGGTCAGCGTCAGTTTCTGCATCGCGCGGGTTACGCCAACGTAGGc > 1:6916831/1‑68 (MQ=255) aCAGACGGCGGGTTTCCGCGTAGGTCAGCGTCAGTTTCTGCATCGCGCGGGTTACGCCAACGTAGGCCAGa > 1:2512693/1‑71 (MQ=255) aCAGACGGCGGGTTTCCGCGTAGGTCAGCGTCAGTTTCTGCATCGCGCGGGTTACGCCAACGTAGGCCAGa > 1:5100328/1‑71 (MQ=255) aCAGACGGCGGGTTTCCGCGTAGGTCAGCGTCAGTTTCTGCATCGCGCGGGTTACGCCAACGTAGGCCAGa > 1:5793241/1‑71 (MQ=255) gACGGCGGGTTTCCGCGTAGGTCAGCGTCAGTTTCTGCATCGCGCGGGTTACGCCAACGTAGGCCAGACgg < 1:2563662/71‑1 (MQ=255) gACGGCGGGTTTCCGCGTAGGTCAGCGTCAGTTTCTGCATCGCGCGGGTTACGCCAACGTAGGCCAGACgg < 1:4550475/71‑1 (MQ=255) gACGGCGGGTTTCCGCGTAGGTCAGCGTCAGTTTCTGCATCGCGCGGGTTACGCCAACGTAGGCCAGACgg < 1:5828141/71‑1 (MQ=255) gACGGCGGGTTTCCGCGTAGGTCAGCGTCAGTTTCTGCATCGCGCGGGTTACGCCAACGTAGGCCAGACgg < 1:4558592/71‑1 (MQ=255) gACGGCGGGTTTCCGCGTAGGTCAGCGTCAGTTTCTGCATCGCGCGGGTTACGCCAACGTAGGCCAGACgg > 1:6547506/1‑71 (MQ=255) gACGGCGGGTTTCCGCGTAGGTCAGCGTCAGTTTCTGCATCGCGCGGGTTACGCCAACGTAGGCCAGACg > 1:2790699/1‑70 (MQ=255) aCGGCGGGTTTCCGCGTAGGTCAGCGTCAGTTTCTGCATCGCGCGGGTTACGCCAACGTAGGCCAGa > 1:5308411/1‑67 (MQ=255) aCGGCGGGTTTCCGCGTAGGTCAGCGTCAGTTTCTGCATCGCGCGGGTTACGCCAACGTAGGCCAGa > 1:5188131/1‑67 (MQ=255) aCGGCGGGTTTCCGCGTAGGTCAGCGTCAGTTTCTGCATCGCGCGGGTTACGCCAACGTAGGCCAGACGGc > 1:323941/1‑71 (MQ=255) aCGGCGGGTTTCCGCGTAGGTCAGCGTCAGTTTCTGCATCGCGCGGGTTACGCCAACGTAGGCAAGa > 1:3960379/1‑67 (MQ=255) cggGTTTCCGCGTAGGTCAGCGTCAGTTTCTGCATCGCGCGGGTTACGCCAACGTAGGCCAGACGGCGttc < 1:248960/71‑1 (MQ=255) gggTTTCCGCGTAGGTCAGCGTCAGTTTCTGCATCGCGCGGGTTACGCCAACGTATGCCAGACGGCGttct < 1:212687/71‑1 (MQ=255) ggTTTCCGCGTAGGTCAGCGTCAGTTTCTGCATCGCGCGGGTTACGCCAAc < 1:5555471/51‑1 (MQ=255) ggTTTCCGCGTAGGTCAGCGTCAGTTTCTGCATCGCGCGGGTTACGCCAAc < 1:549680/51‑1 (MQ=255) ggTTTCCGCGTAGGTCAGCGTCAGTTTCTGCATCGCGCGGGTTACGCCAAc < 1:1402018/51‑1 (MQ=255) ggTTTCCGCGTAGGTCAGCGTCAGTTTCTGCATCGCGCGGGTTACGCCAAc < 1:932196/51‑1 (MQ=255) ggTTTCCGCGTAGGTCAGCGTCAGTTTCTGCATCGCGCGGGTTACGCCAAc < 1:1770850/51‑1 (MQ=255) ggTTTCCGCGTAGGTCAGCGTCAGTTTCTGCATCGCGCGGGTTACGCCAAc < 1:2073617/51‑1 (MQ=255) gTTTCCGCGTAGGTCAGCGTCAGTTTCTGCATCGCGCGGGTTACGCCAACGTAGGCCa > 1:1134886/1‑58 (MQ=255) gTTTCCGCGTAGGTCAGCGTCAGTTTCTGCATCGCGCGGGTTACGCCAACGTAGGCCa > 1:6148910/1‑58 (MQ=255) gTTTCCGCGTAGGTCAGCGTCAGTTTCTGCATCGCGCGGGTTACGCCAACGTAGGCCAGACGGCGttcttc > 1:3610655/1‑71 (MQ=255) gTTTCCGCGTAGGTCAGCGTCAGTTTCTGCATCGCGCGGGTTACGCCAACGTAGGCCAGACGGCGttcttc < 1:1963732/71‑1 (MQ=255) tCCGCGTAGGTCAGGACCAGTCGCTGCATGGCGCGGGTGACGCCGACGTAGGCCAGGCGGCGttcctcttc > 1:2359371/1‑71 (MQ=255) tCCGCGTAGGTCAGGACCAGTCGCTGCATGGCGCGGGTGACGCCGACGTAGGCCAG‑CGGCGttcctcttc > 1:6901060/1‑70 (MQ=255) cgcgTAGGTCAGGACCAGTCGCTGCATGGCGCGGGTGACGCCGACGTAGGCCAGGCGGCGTTCCTCTTcc < 1:6840589/70‑1 (MQ=255) cgTAGGTCAGCGTCAGTTTCTGCATCGCGAGGGTTACGCCAACGTAGGCCAGACGGCGTTCTTCTTCCAGa < 1:3601821/71‑1 (MQ=255) tAGGTCAGCGTCAGTTTCTGCATCGCGCGGGTTACGCCAACGTAGGCCAGACgg < 1:4887395/54‑1 (MQ=255) tAGGTCAGCGTCAGTTTCTGCATCGCGCGGGTTACGCCAACGTAGGCCAGACgg < 1:6572549/54‑1 (MQ=255) tAGGTCAGCGTCAGTTTCTGCATCGCGCGGGTTACGCCAACGTAGGCCAGACgg < 1:2257306/54‑1 (MQ=255) tAGGTCAGCGTCAGTTTCTGCATCGCGCGGGTTACGCCAACGTAGGCCAGACgg < 1:5308320/54‑1 (MQ=255) tAGGTCAGCGTCAGTTTCTGCATCGCGCGGGTTACGCCAACGTAGGCCAGACgg < 1:2259476/54‑1 (MQ=255) aGGTCAGCGTCAGTTTCTGCATCGCGCGGGTTACGCCAACGTAGGCCAGACgg < 1:3618050/53‑1 (MQ=255) aGGTCAGCGTCAGTTTCTGCATCGCGCGGGTTACGCCAACGTAGGCCAGACGGCGTTCTTCTTCCAGAcgc > 1:3806840/1‑71 (MQ=255) aGGTCAGCGTCAGTTTCTGCATCGCGCGGGTTACGCCAACGTAGGCCAGACGGCGTTCTTCTTCCAGAcgc > 1:5484784/1‑71 (MQ=255) ggTCAGCGTCAGTTTCTGCATCGCGCGGGTTACGCCAACGTAGGCCAGACGGCg > 1:6359111/1‑54 (MQ=255) ggTCAGCGTCAGTTTCTGCATCGCGCGGGTTACGCCAACGTAGGCCAGACGGCg > 1:6498665/1‑54 (MQ=255) ggTCAGCGTCAGTTTCTGCATCGCGCGGGTTACGCCAACGTAGGCCAGACGGCg > 1:753848/1‑54 (MQ=255) ggTCAGCGTCAGTTTCTGCATCGCGCGGGTTACGCCAACGTAGGCCAGACGGCg > 1:5143639/1‑54 (MQ=255) ggTCAGCGTCAGTTTCTGCATCGCGCGGGTTACGCCAACGTAGGCCAGACGGCg > 1:3231702/1‑54 (MQ=255) gCGTCAGTTTCTGCATCGCGCGGGTTACGCCAACGTAGGCCAGACGGCGTTCTTCTTCCAGACGCCCGCCt < 1:3224354/71‑1 (MQ=255) aGTTTCTGCATCGCGCGGGTTACGCCAACGTAGGCCAGACGGCGTTCTTCTTCCAGACGCCCGCCTTCATc > 1:2003411/1‑71 (MQ=255) aGTTTCTGCATCGCGCGGGTTACGCCAACGTAGGCCAGACGGCGTTCTTCATCCAGAcgcccg > 1:3115943/1‑63 (MQ=255) gTTTCTGCATCGCGCGGGTTACGCCAACGTAGGCCAGACGGCGTTCTTCTTCCAGACGCCCGCCTTCATc > 1:6658080/1‑70 (MQ=255) tCTGCATCGCGCGGGTTACGCCAACGTAGGCCAGACGGCGTTCTTCTTCCAGAcgcccgcc > 1:4769020/1‑61 (MQ=255) cTGCATCGCGCGGGTTACGCCAACGTAGGCCAGACGGCGTTCTTCTTCCAGACGCCCGCCTTCATCCAg > 1:729793/1‑69 (MQ=255) gCATCGCGCGGGTTACGCCAACGTAGGCCAGACGGCGTTCTTCTTCCAGACGCCCGCCTTCATCCAGCGa > 1:3922935/1‑70 (MQ=255) aTCGCGCGGGTTACGCCAACGTAGGCCAGACGGCGTTCTTCTTCCAGACGCCCGCCTTCATCCAGCGACa > 1:3741660/1‑70 (MQ=255) tCGCGCGGGTTACGCCAACGTAGGCCAGACGGCGTTCTTCTTCCa < 1:263849/45‑1 (MQ=255) tCGCGCGGGTTACGCCAACGTAGGCCAGACGGCGTTCTTCTTCCa < 1:854979/45‑1 (MQ=255) gcgcgGGTTACGCCAACGTAGGCCAGACGGCGTTCTTCTTCCAGACGCCCGCCTTCATCCAGCGACATCt < 1:4921056/70‑1 (MQ=255) ggTTACGCCAACGTAGGCCAGACGGCGTTCTTCTTCCAGACGCCCGCCTTCATCCAGCGACATCTGGCTTg < 1:141470/71‑1 (MQ=255) ggTGACGCCGACGTAGGCCAGGCGGCGTTCCTCTTcc < 1:2203560/37‑1 (MQ=25) gTTACGCCAACGTAGGCCAGACGGCGTTCTTCTTCCAGAcgc < 1:331244/42‑1 (MQ=255) tACGCCAACGTAGGCCAGACGGCGTTCTTCTTCCAGACGCCCGCCTTCATCCAGCGACAt > 1:3211128/1‑60 (MQ=255) tACGCCAACGTAGGCCAGACGGCGTTCTTCTTCCAGACGCCCGCCTTCATCCAGCGACAt > 1:5482031/1‑60 (MQ=255) tACGCCAACGTAGGCCAGACGGCGTTCTTCTTCCAGACGCCCGCCTTCATCCAGCGACATCTGGCTTggg > 1:951131/1‑70 (MQ=255) | GTAAACCTCTTTACCATACAGACGGCGGGTTTCCGCGTAGGTCAGCGTCAGTTTCTGCATCGCGCGGGTTACGCCAACGTAGGCCAGACGGCGTTCTTCTTCCAGACGCCCGCCTTCATCCAGCGACATCTGGCTTGGG > Exported/2398753‑2398891 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |