Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	Exported	2,432,736	G→A	5.3%	I39I (ATC→ATT)	trxA ←	thioredoxin 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	Exported	2,432,736	0	G	A	5.3%	322.7 / 5.5	94	I39I (ATC→ATT)	trxA	thioredoxin 1
Reads supporting (aligned to +/- strand):  ref base G (56/33);  new base A (3/2);  total (59/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.43e-01

TCGATGTTCAGTTTTGCAACGGTCAGTTTGCCCTGATATTCGTCAGCGATTTCATCCAGAATCGGGGCGATCATTTTGCACGGACCGCACCACTCTGCCCAGAAATCGACGAGGATCGCCCCGTCCGCTTTGAGTACAT  >  Exported/2432668‑2432806                                                                     |                                                                       tCGATGTTCAGTTTTGCAACGGTCAGTTTGCCCTGATATTCGTCAGCGATTTCATCCAGAATCGGGGCGAt                                                                      <  1:4200342/71‑1 (MQ=255)  cGATGTTCAGTTTTGCAACGGTCAGTTTGCCCTGATATTCGTCAGCGATTTCATCCAGAATCGGGGCGAt                                                                      >  1:1354712/1‑70 (MQ=255)  cGATGTTCAGTTTTGCAACGGTCAGTTTGCCCTGATATTCGTCAGCGATTTCATCCAGAATCGGGGCGAt                                                                      >  1:1460795/1‑70 (MQ=255)  cGATGTTCAGTTTTGCAACGGTCAGTTTGCCCTGATATTCGTCAGCGATTTCATCCAGAATCGGGGCGAt                                                                      >  1:4247632/1‑70 (MQ=255)    aTGTTCAGTTTTGCAACGGTCAGTTTGCCCTGATATTCGTCAGCGATTTCATCCAGAATCGGGGCGATCAt                                                                   <  1:6212016/71‑1 (MQ=255)    aTGTTCAGTTTTGCAACGGTCAGTTTGCCCTGATATTCGTCAGCGATTTCATCCAGAATCGGGGCGATCAt                                                                   <  1:2481638/71‑1 (MQ=255)    aTGTTCAGTTTTGCAACGGTCAGTTTGCCCTGATATTCGTCAGCGATTTCATCCAGAATCGGGGCGATCAt                                                                   <  1:6406519/71‑1 (MQ=255)     tGTTCAGTTTTGCAACGGTCAGTTTGCCCTGATATTCGTCAGCGATTTCATCCAGAATCGGGGCGATCAtt                                                                  >  1:1333984/1‑71 (MQ=255)     tGTTCAGTTTTGCAACGGTCAGTTTGCCCTGATATTCGTCAGCGATTTCATCCAGAATCGGGGCGATCAtt                                                                  >  1:6409295/1‑71 (MQ=255)       ttCAGTTTTGCAACGGTCAGTTTGCCCTGATATTCGTCAGCGATTTCATCCAGAATCGGGGCGATCAtttt                                                                <  1:4164896/71‑1 (MQ=255)         cAGTTTTGCAACGGTCAGTTTGCCCTGATATTCGTCAGCGATTTCATCCAGAATCGGGGCGATCAtttt                                                                >  1:2881425/1‑69 (MQ=255)         cAGTTTTGCAACGGTCAGTTTGCCCTGATATTCGTCAGCGATTTCATCCAGAATCGGGGCGATCATTTTg                                                               <  1:4807744/70‑1 (MQ=255)         cAGTTTTGCAACGGTCAGTTTGCCCTGATATTCGTCAGCGATTTCATCCAGAATCGGGGCGATCATTTTg                                                               <  1:1058218/70‑1 (MQ=255)          aGTTTTGCAACGGTCAGTTTGCCCTGATATTCGTCAGCGATTTCATCCAGAATCGGGGCGATCATTTTg                                                               <  1:3234939/69‑1 (MQ=255)          aGTTTTGCAACGGTCAGTTTGCCCTGATATTCGTCAGCGATTTCATCCAGAATCGGGGCGATCATTTTg                                                               <  1:1823576/69‑1 (MQ=255)            ttttGCAACGGTCAGTTTGCCCTGATATTCGTCAGCGATTTCATCCAGAATCGGGGCGATCATTTTGCACg                                                           <  1:2175711/71‑1 (MQ=255)             tttGCAACGGTCAGTTTGCGCTGATATTCGTCAGCGATTTCATCCAGAATCGGGGCGATCATTTTGCACg                                                           >  1:3810735/1‑70 (MQ=255)             tttGCAACGGTCAGTTTGCCCTGATATTCGTCAGCGATTTCATCCAGAATCGGGGCGATCATTTTGCACg                                                           >  1:523211/1‑70 (MQ=255)             tttGCAACGGTCAGTTTGCCCTGATATTCGTCAGCGATTTCATCCAGAATCGGGGCGATCATTTTGCACg                                                           >  1:2895492/1‑70 (MQ=255)                 cAACGGTCAGTTTGCCCTGATATTCGTCAGCGATTTCATCCAGAATCGGGGCGATCATTTTGCACGGAcc                                                       <  1:4971117/70‑1 (MQ=255)                   aCGGTCAGTTTGCCCTGATATTCGTCAGCGATTTCATCCAGAATCGGGGCGATCATTTTGCACGGACCGca                                                    >  1:384752/1‑71 (MQ=255)                   aCGGTCAGTTTGCCCTGATATTCGTCAGCGATTTCATCCAGAATCGGGGCGATCATTTTGCACGGACCGca                                                    >  1:3594657/1‑71 (MQ=255)                   aCGGTCAGTTTGCCCTGATATTCGTCAGCGATTTCATCCAGAATCGGGGCGATCATTTTGCACGGACCGca                                                    >  1:4693778/1‑71 (MQ=255)                     ggTCAGTTTGCCCTGATATTCGTCAGCGATTTCATCCAGAATCGGGGCGATCATTTTGCACGGACCGcac                                                   >  1:153735/1‑70 (MQ=255)                     ggTCAGTTTGCCCTGATATTCGTCAGCGATTTCATCCAGAATCGGGGCGATCATTTTGCACGGACCGcac                                                   >  1:4512591/1‑70 (MQ=255)                     ggTCAGTTTGCCCTGATATTCGTCAGCGATTTCATCCAGAATCGGGGCGATCATTTTGCACGGACCGcac                                                   >  1:2247126/1‑70 (MQ=255)                     ggTCAGTTTGCCCTGATATTCGTCAGCGATTTCATCCAGAATCGGGGCGATCATTTTGCACGGACCGcac                                                   >  1:1761980/1‑70 (MQ=255)                     ggTCAGTTTGCCCTGATATTCGTCAGCGATTTCATCCAGAATCGGGGCGATCATTTTGCACGGACCGcac                                                   >  1:2517074/1‑70 (MQ=255)                     ggTCAGTTTGCCCTGATATTCGTCAGCGATTTCATCCAGAATCGGGGCGATCATTTTGCACGGACCGcac                                                   >  1:1778734/1‑70 (MQ=255)                      gTCAGTTTGCCCTGATATTCGTCAGCGATTTCATCCAGAATCGGGGCGATCAtttt                                                                <  1:4519503/56‑1 (MQ=255)                       tCAGTTTGCCCTGATATTCGTCAGCGATTTCATCCAGAATCGGGGCGATCATTTTGCACGGACCGcaccac                                                <  1:1471248/71‑1 (MQ=255)                         aGTTTGCCCTGATATTCGTCAGCGATTTCATCCAGAATCGGGGCGATCATTTTGCACGGACCGCACCActc                                              >  1:6797327/1‑71 (MQ=255)                         aGTTTGCCCTGATATTCGTCAGCGATTTCATCCAGAATCGGGGCGATCATTTTGCACGGACCGCACCAct                                               <  1:5376043/70‑1 (MQ=255)                             tGCCCTGATATTCGTCAGCGATTTCATCCAGAATCGGGGCGATCATTTTGCACGGACCGCACCACTCTGcc                                          >  1:938998/1‑71 (MQ=255)                                  tGATATTCGTCAGCGATTTCATCCAGAATCGGGGCGATCATTTTGCACGGACCGCACCACTCTGCCCAGa                                      >  1:1662793/1‑70 (MQ=255)                                  tGATATTCGTCAGCGATTTCATCCAGAATCGGGGCGATCATTTTGCACGGACCGCACCACTCTGCCCAGa                                      >  1:5139522/1‑70 (MQ=255)                                  tGATATTCGTCAGCGATTTCATCCAGAATCGGGGCGATCATTTTGCACGGACCGCACAACTCTGCCCAGa                                      >  1:4557844/1‑70 (MQ=255)                                   gATATTCGTCAGCGATTTCATCCAGAATCGGGGCGATCAtt                                                                  <  1:5164318/41‑1 (MQ=255)                                    atatTCGTCAGCGATTTCATCCAGAATCGGGGCGATCATTTTGCACGGACCGCACCACTCTGCCCAGaaa                                    <  1:5482392/70‑1 (MQ=255)                                    atatTCGTCAGCGATTTCATCCAGAATCGGGGCGATCATTTTGCACGGACCGCACCACTCTGCCCAGAAAt                                   >  1:6322139/1‑71 (MQ=255)                                     tatTCGTCAGCGATTTCATCCAGAATCGGGGCGATCATTTTGCACGGACCGCACCACTCTGCCCAGAAATc                                  <  1:1923800/71‑1 (MQ=255)                                     tatTCGTCAGCGATTTCATCCAGAATCGGGGCGATCATTTTGCACGGACCGCACCACTCTGCCCAGAAATc                                  <  1:1605802/71‑1 (MQ=255)                                      atTCGTCAGCGATTTCATCCAGAATCGGGGCGATCATTTTGCACGGACCTCACCACTCTGCCCAGaa                                     <  1:2435066/67‑1 (MQ=255)                                       ttCGTCAGCGATTTCATCCAGAATCGGGGCGATCATTTTGCACGGACCGCACCACTCTGCCCAGAAATcga                                >  1:6600835/1‑71 (MQ=255)                                        tCGTCAGCGATTTCATCCAGAATCGGGGCGATCATTTTGCACGGACCGCACCACTCTGCCCAGAAATcgac                               >  1:5278249/1‑71 (MQ=255)                                        tCGTCAGCGATTTCATCCAGAATCGGGGCGATCATTTTGCACGGACCGCACCACTCTGCCCAGAAATcgac                               >  1:3516058/1‑71 (MQ=255)                                         cGTCAGCGATTTCATCCAGAATCGGGGCGATCATTTTGCa                                                             >  1:442608/1‑40 (MQ=255)                                         cGTCAGCGATTTCATCCAGAATCGGGGCGATCATTTTGCa                                                             >  1:645760/1‑40 (MQ=255)                                         cGTCAGCGATTTCATCCAGAATCGGGGCGATCATTTTGCa                                                             >  1:4868466/1‑40 (MQ=255)                                         cGTCAGCGATTTCATCCAGAATCGGGGCGATCATTTTGCa                                                             >  1:3694484/1‑40 (MQ=255)                                         cGTCAGCGATTTCATCCAGAATCGCGGCGATCATTTTGCa                                                             >  1:2991461/1‑40 (MQ=255)                                          gTCAGCGATTTCATCCAGAATCGGGGCGATCATTTTGCACGGACCGCACCACTCTGCCCAGAAATcgacg                              <  1:5412400/70‑1 (MQ=255)                                             aGCGATTTCATCCAGAATCGGGGCGATCATTTTGCACGGACCGCACCACTCTGCCCAGAAATCGACGAgg                           <  1:1948317/70‑1 (MQ=255)                                             aGCGATTTCATCCAGAATCGGGGCGATCATTTTGCACGGACCGCACCACTCTGCCCAGAAATCGACGAgg                           <  1:5357427/70‑1 (MQ=255)                                                gATTTCATCCAGAATCGGGGCGATCATTTTGCACGGACCg                                                      >  1:2152335/1‑40 (MQ=255)                                                gATTTCATCCAGAATCGGGGCGATCATTTTGCACGGACCg                                                      >  1:6399522/1‑40 (MQ=255)                                                gATTTCATCCAGAATCGGGGCGATCATTTTGCACGGACCGCACCACTCTGCCCAGAAATCGACGAgg                           >  1:35852/1‑67 (MQ=255)                                                gATTTCATCCAGAATCGGGGCGATCATTTTGCACGGACCGCACCACTCTGCCCAGAAATCGACGAgg                           >  1:3402237/1‑67 (MQ=255)                                                gATTTCATCCAGAATCGGGGCGATCATTTTGCACGGACCGCACCACTCTGCCCAGAAATCGACGAgg                           >  1:1820562/1‑67 (MQ=255)                                                gATTTCATCCAGAATCGGGGCGATCATTTTGCACGGACCGCACCACTCTGCCCAGAAATCGACGAg                            >  1:972692/1‑66 (MQ=255)                                                gATTTCATCCAGAATCGGGGCGATCATTTTGCACGGACCGCACCACTCTGCCCAGAAATCGACGAGGATCg                       <  1:6070039/71‑1 (MQ=255)                                                 aTTTCATCCAGAATCGGGGCGATCATTTTGCACGGACCGCACCACTCTGCCCAGAAATCGACGAGGATCGc                      <  1:3291053/71‑1 (MQ=255)                                                 aTTTCATCCAGAATCGGGGCGATCATTTTGCACGGACCGCACCACTCTGCCCAGAAATCGACGAGGATCGc                      <  1:2532160/71‑1 (MQ=255)                                                  tttCATCCAGAATCGGGGCGATCATTTTGCACGGACCGc                                                     >  1:1990091/1‑39 (MQ=255)                                                  tttCATCCAGAATCGGGGCGATCATTTTGCACGGACCGc                                                     >  1:6131623/1‑39 (MQ=255)                                                  tttCATCCAGAATCGGGGCGATCATTTTGCACGGACCGCACCACTCTGCCCAGAAATCGACGAGGATCGcc                     >  1:3597575/1‑71 (MQ=255)                                                  tttCATCCAGAATCGGGGCGATCATTTTGCACGGACCGCACCACTCTGCCCAGAAATCGACGAGGATCGcc                     >  1:1699938/1‑71 (MQ=255)                                                  tttCATCCAGAATCGGGGCGATCATTTTGCACGGACCGCACCACTCTGCCCAGAAATCGACGAGGATCGcc                     >  1:3125715/1‑71 (MQ=255)                                                  tttCATCCAGAATCGGGGCGATCATTTTGCACGGACCGCACCACTCTGCCCAGAAATCGACGAGGATCGcc                     >  1:3037692/1‑71 (MQ=255)                                                  tttCATCCAGAATCGGGGCGATCATTTTGCACGGACCGCACCACTCTGCCCAGAAATCGACGAGGATCGcc                     >  1:5424877/1‑71 (MQ=255)                                                   ttcttcCAGGATCGGGGCAATCATCTTGCAAGGACCGcacc                                                  <  1:6233763/41‑1 (MQ=19)                                                   ttcttcCAGGATCGGGGCAATCATCTTGCAAGGACCGcacc                                                  <  1:3396147/41‑1 (MQ=19)                                                   ttCATCCAGAATCGGGGCGATCATTTTGCACGGACCGCACCACTCTGCCCAGAAATcga                                >  1:6500999/1‑59 (MQ=255)                                                   ttCATCCAGAATCGGGGCGATCATTTTGCACGGACCGCACCACTCTGCCCAGAAATCGACGAGGATCGccc                    >  1:410147/1‑71 (MQ=255)                                                   ttCATCCAGAATCGGGGCGATCATTTTGCACGGACCGCACCACTCTGCCCAGAAATCGACGAGGATCGccc                    >  1:6837776/1‑71 (MQ=255)                                                   ttCATCCAGAATCGGGGCGATCATTTTGCACGGACCGCACCACTCTGCCCAGAAATCGACGAGGATCGccc                    >  1:161159/1‑71 (MQ=255)                                                     cttCCAGGATCGGGGCAATCATCTTGCAAGGACCGcaccac                                                >  1:1499214/3‑41 (MQ=19)                                                     cttCCAGGATCGGGGCAATCATCTTGCAAGGACCGcaccac                                                >  1:6848461/3‑41 (MQ=19)                                                     cttCCAGGATCGGGGCAATCATCTTGCAAGGACCGcaccac                                                >  1:1665947/3‑41 (MQ=19)                                                     cATCCAGAATCGGGGCGATCATTTTGCACGGACCGCACCACTCTGCCCAGAAATCGACGAGGATCGcccc                   >  1:3450008/1‑70 (MQ=255)                                                     cATCCAGAATCGGGGCGATCATTTTGCACGGACCGCACCACTCTGCCCAGAAATCGACGAGGATCGcccc                   <  1:1672537/70‑1 (MQ=255)                                                     cATCCAGAATCGGGGCGATCATTTTGCACGGACCGCACCACTCTGCCCAGAAATCGACGAGGATCGcccc                   <  1:6783380/70‑1 (MQ=255)                                                      aTCCAGAATCGTGGCGATCATTTTGCACGGACCGCACCACTCTGCCCAGAAATcga                                <  1:1533356/56‑1 (MQ=255)                                                      aTCCAGAATCGGGGCGATCATTTTGCACGGACCGCACCACTCTGCCCAGAAATcga                                <  1:3649687/56‑1 (MQ=255)                                                          aGAATCGGGGCGATCATTTTGCACGGACCGCACCACTCTGCCCAGAAATCGACGAg                            >  1:2097998/1‑56 (MQ=255)                                                             aTCGGGGCGATCATTTTGCACGGACCGCACCACTCTGCCCAGAAATCGACGAGGATCGCCCCGTCCGCttt          <  1:5267096/71‑1 (MQ=255)                                                             aTCGGGGCGATCATTTTGCACGGACCGCACCACTCTGCCCAGAAATCGACGAGGATCGCCCCGTCCGCttt          <  1:4746724/71‑1 (MQ=255)                                                             aTCGGGGCGATCATTTTGCACGGACCGCACCACTCTGCCCAGAAATCGACGAGGATCGCCCCGTCCGCttt          <  1:6723745/71‑1 (MQ=255)                                                             aTCGGGGCGATCATTTTGCACGGACCGCACCACTCTGCCCAGAAATCGACGAGGATCGCCCCGTCCGCttt          <  1:3176627/71‑1 (MQ=255)                                                              tCGGGGCGATCATTTTGCACGGACCGCACCACTCTGCCCAGaa                                     >  1:3561665/1‑43 (MQ=255)                                                              tCGGGGCGATCATTTTGCACGGACCGCACCACTCTGCCCAGAAATCGACGAg                            >  1:4895707/1‑52 (MQ=255)                                                              tCGGGGCGATCATTTTGCACGGACCGCACCACTCTGCCCAGAAATCGACGAg                            >  1:5120585/1‑52 (MQ=255)                                                              tCGGGGCGATCATTATGCACGGACCGCACCACTCTGCCCAGAAATcgacg                              >  1:1633515/1‑50 (MQ=255)                                                                    cGATCATTTTGCACGGACCGCACCACTCTGCCCAGAAATCGACGAGGATCGCCCCGTCCGCTTTGa        >  1:1558568/1‑66 (MQ=255)                                                                     gATCATTTTGCACGGACCGCACCACTCTGCCCAGAAATCGACGAGGATCGCCCCGTCCGCTTTGAGTACAt  >  1:5142259/1‑71 (MQ=255)                                                                     |                                                                       TCGATGTTCAGTTTTGCAACGGTCAGTTTGCCCTGATATTCGTCAGCGATTTCATCCAGAATCGGGGCGATCATTTTGCACGGACCGCACCACTCTGCCCAGAAATCGACGAGGATCGCCCCGTCCGCTTTGAGTACAT  >  Exported/2432668‑2432806

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑