Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	Exported	62,484	T→C	5.3%	K325K (AAA→AAG)	polB ←	DNA polymerase II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	Exported	62,484	0	T	C	5.3%	233.1 / 3.6	76	K325K (AAA→AAG)	polB	DNA polymerase II
Reads supporting (aligned to +/- strand):  ref base T (50/22);  new base C (2/2);  total (52/24)
Fisher's exact test for biased strand distribution p-value = 5.87e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.88e-01

TGATTTCAGTTTTGTGGAAGATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTTGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGTCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGC  >  Exported/62414‑62554                                                                       |                                                                       tGATTTCAGTTTTGTGGAAGATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTTGCCAGCGCAGGt                                                                        >  1:1031841/1‑71 (MQ=255) tGATTTCAGTTTTGTGGAAGATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTTGCCAGCGCAGGt                                                                        <  1:4466893/71‑1 (MQ=255) tGATTTCAGTTTTGTGGAAGATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTTGCCAGCGCAGGt                                                                        <  1:848712/71‑1 (MQ=255) tGATTTCAGTTTTGTGGAAGATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTTGCCAGCGCAGGt                                                                        >  1:1933420/1‑71 (MQ=255) tGATTTCAGTTTTGTGGAAGATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTTGCCAGCGCAGGt                                                                        <  1:252925/71‑1 (MQ=255)  gATTTCAGTTTTGTGGAAGATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTTGCCAGCGCAGGtt                                                                       >  1:5307856/1‑71 (MQ=255)        aGTTTTGTGGAAGATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTTGCCAGCGCAGGTTTATCt                                                                  <  1:6394136/70‑1 (MQ=255)            ttGTGGAAGATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTTGCCAGCGCAGGTTTATCTTCGGc                                                             >  1:2461105/1‑71 (MQ=255)             tgtgGAAGATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTTGCCAGCGCAGGTTTAt                                                                    >  1:1799094/1‑63 (MQ=255)             tgtgGAAGATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTTGCCAGCGCAGGTTTATCTTCGGc                                                             <  1:4014366/70‑1 (MQ=255)             tgtgGAAGATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTTGCCAGCGCAGGTTTATCTTCGGc                                                             >  1:808554/1‑70 (MQ=255)               tgGAAGATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTTGCCAGCGCAGGTTTATCTTCGGCGa                                                           <  1:2167280/70‑1 (MQ=255)                ggAAGATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTTGCCAGCGCAGGTTTATCTTCGGCGa                                                           >  1:2489917/1‑69 (MQ=255)                 gAAGATGCGCGTGACCAGCTCGCAATCCTTGAGGTTGTACCAGGCCAGCGCCGGCTTGTCCTCGGCGaaca                                                        >  1:241191/1‑69 (MQ=255)                    gATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTTGCCAGCGCAGGTTTATCTTCGGCGaaa                                                         <  1:5216757/67‑1 (MQ=255)                    gATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTTGCCAGCGCAGGTTTATCTTCGGCGAAAcggc                                                     >  1:6362388/1‑71 (MQ=255)                    gATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTTGCCAGCGCAGGTTTATCTTCGGCGAAAcggc                                                     >  1:1253820/1‑71 (MQ=255)                      tCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTTGCCAGCGCAGGTTTAt                                                                    >  1:1082083/1‑54 (MQ=255)                      tCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTTGCCAGCGCAGGTTTAt                                                                    >  1:5266919/1‑54 (MQ=255)                      tCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTTGCCAGCGCAGGTTTATCTTCGGCGAAAcggcgg                                                   >  1:3210811/1‑71 (MQ=255)                      tCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTTGCCAGCGCAGGTTTATCTTCGGCGAAAcggcgg                                                   >  1:1019963/1‑71 (MQ=255)                      tCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTTGCCAGCGCAGGTTTATCTTCGGCGAAAcggcgg                                                   >  1:5958180/1‑71 (MQ=255)                      tCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTTGCCAGCGCAGGTTTATCTTCGGCGAAAcggcgg                                                   >  1:6150460/1‑71 (MQ=255)                       cTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTTGCCCGCGCAGGTTTATCTTCGGCGAAACGGCGGt                                                  >  1:4736683/1‑71 (MQ=255)                       cTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTTGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGt                                                  >  1:607650/1‑71 (MQ=255)                       cTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTTGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGt                                                  >  1:932236/1‑71 (MQ=255)                       cTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTTGCCAGCGCAGGTTTATCTTCGGCGAAACGGCCGt                                                  >  1:6365210/1‑71 (MQ=255)                          cGTGACCAGCTCGCAATCCTTGAGGTTGTACCAGGCCAGCGCCGGCTTGTCCTCGGCGAACATGCGGTcga                                               <  1:1547846/71‑3 (MQ=255)                              aCCAGCTCGCAATCTTTCAGGTTATAAGTTGCCAGCGCAGGTTTATCTTCGGCg                                                            >  1:4784393/1‑54 (MQ=255)                              aCCAGCTCGCAATCTTTCAGGTTATAAGTTGCCAGCGCAGGTTTATCTTCGGCg                                                            >  1:4776020/1‑54 (MQ=255)                              aCCAGCTCGCAATCTTTCAGGTTATAAGTTGCCAGCGCAGGTTTATCTTCGGCg                                                            >  1:3229676/1‑54 (MQ=255)                              aCCAGCTCGCAATCCTTGAGGTTGTACCAGGCCAGCGCCGGCTTGTCCTCGGCGAACATGCGGTCGAtctc                                           >  1:2422970/1‑71 (MQ=255)                               ccAGCTCGCAATCTTTCAGGTTATAAGTTGCCAGCGCAGGTTTATCTTCGGCGa                                                           <  1:2914662/54‑1 (MQ=255)                               ccAGCTCGCAATCCTTGAGGTTGTACCAGGCCAGCGCCGGCTTGTCCTCGGCGAACATGCGGTCGATCTCg                                          <  1:2096503/71‑1 (MQ=255)                                 aGCTCGCAATCTTTCAGGTTATAAGTTGCCAGCGCAGGTTTATCTTCGGc                                                             >  1:2484461/1‑50 (MQ=255)                                  gCTCGCAATCTTTCAGGTTATAAGTTGCCAGCGCAGGTTTATc                                                                   <  1:191269/43‑1 (MQ=255)                                  gCTCGCAATCTTTCAGGTTATAAGTTGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGTc                                                 >  1:6141302/1‑61 (MQ=255)                                  gCTCGCAATCTTTCAGGTTATAAGTTGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGTc                                                 >  1:6314886/1‑61 (MQ=255)                                  gCTCGCAATCTTTCAGGTTATAAGTTGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGTc                                                 >  1:3597422/1‑61 (MQ=255)                                  gCTCGCAATCTTTCAGGTTATAAGTTGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGTCAATTTCGTc                                        >  1:5993921/1‑70 (MQ=255)                                   cTCGCAATCTTTCAGGTTATAAGTTGCCAGCGCAGGTTTATCTTCGGCGAAACGTCGGTCAATTTCGTCCa                                      <  1:2241917/71‑1 (MQ=255)                                   cTCGCAATCTTTCAGGTTATAAGTTGCCAGCGCAGGTTTATCTTCGGCGAAACGGCTGTCAATTTCGTCCa                                      <  1:2330194/71‑1 (MQ=255)                                   cTCGCAATCTTTCAGGTTATAAGTTGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGTCAATTTCGTCCa                                      <  1:3561457/71‑1 (MQ=255)                                     cGCAATCTTTCAGGTTATAAGTTGCCAGCGCAGGTTTATCTTCGGCGaaa                                                         >  1:4517680/1‑50 (MQ=255)                                     cGCAATCTTTCAGGTTATAAGTTGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGTCaa                                               >  1:6584173/1‑60 (MQ=255)                                     cGCAATCTTTCAGGTTATAAGTTGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGTCaa                                               >  1:5567953/1‑60 (MQ=255)                                      gCAATCTTTCAGGTTATAAGTTGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGTCAATTTCGTCCAt                                     <  1:5604015/69‑1 (MQ=255)                                       cAATCTTTCAGGTTATAAGTTGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGTCAATTTCGTCCATTCg                                  <  1:23250/71‑1 (MQ=255)                                         aTCTTTCAGGTTATAAGTTGCCAGCGCAGGTTTATCTTCGGCGAAAcg                                                       >  1:3756049/1‑48 (MQ=255)                                         aTCTTTCAGGTTATAAGTTGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGTCAATTTCGTcc                                       >  1:601340/1‑64 (MQ=255)                                         aTCTTTCAGGTTATAAGTTGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGTCAATTTCGTcc                                       >  1:5704933/1‑64 (MQ=255)                                          tCTTTCAGGTTATAAGTTGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGTCAATTTCGTCCATTCGATc                               >  1:5338803/1‑71 (MQ=255)                                            tttCAGGTTATAAGTTGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGTCAATTTCGTc                                        <  1:6052000/60‑1 (MQ=255)                                              tCAGGTTATAAGTTGCCAGCGCAGGTTTATCTTCGGCGAAAcg                                                       >  1:2513290/1‑43 (MQ=255)                                              tCAGGTTATAAGTTGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGTCAATTTCGTCCAt                                     >  1:4784659/1‑61 (MQ=255)                                              tCAGGTTATAAGTTGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGTCAATTTCGTCCAt                                     >  1:6651069/1‑61 (MQ=255)                                              tCAGGTTATAAGTTGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGTCAATTTCGTCCATTCGATcc                              <  1:5823581/68‑1 (MQ=255)                                              tCAGGTTATAAGTTGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGTCAATTTCGTCCATTCGATCCCa                            <  1:2528479/70‑1 (MQ=255)                                              tCAGGTTATAAGTTGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGTCAATTTCGTCCATTCGATCCCa                            <  1:2566889/70‑1 (MQ=255)                                                 ggTTATAAGTTGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGTCAATTTCGTCCATTCGATCCCACggg                        <  1:198289/71‑1 (MQ=255)                                                     ataAGTTGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGTCAATTTCGTCCATTCGATCCCACGGGTTa                     >  1:3036434/1‑70 (MQ=255)                                                     ataAGTTGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGTCAATTTCGTCCATTCGATCCCACGGGTTa                     >  1:178466/1‑70 (MQ=255)                                                     ataAGTTGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGTCAATTTCGTCCATTCGATCCCACGGGTTa                     <  1:2125439/70‑1 (MQ=255)                                                     ataAGTTGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGTCAATTTCGTCCATTCGATCCCACGGGTTAt                    >  1:2905641/1‑71 (MQ=255)                                                     ataAGTTGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGTCAATTTCGTCCATTCGATCCCACGGGTTAt                    >  1:1854288/1‑71 (MQ=255)                                                      taAGTTGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGTCAATTTCGTCCATTCGATCCCACGGGTTATc                   <  1:5432507/71‑1 (MQ=255)                                                       aaGTTGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGTCAATTTCGTCCATTCGATCCCACGGGTTATc                   >  1:2200664/1‑70 (MQ=255)                                                       aaGTTGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGTCAATTTCGTCCATTCGATCCCACGGGTTATc                   >  1:1007033/1‑70 (MQ=255)                                                        aGTTGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGTCAATTTCGTCCATTCGATCCCACGGGTTATCGa                 >  1:6806464/1‑71 (MQ=255)                                                             ccAGCGCAGGTTTATCTTCGGCGAAACGGCGGTCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGa             <  1:6546488/70‑1 (MQ=255)                                                             ccAGCGCAGGTTTATCTTCGGCGAAACGGCGGTCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGa             <  1:950454/70‑1 (MQ=255)                                                             ccAGCGCAGGTTTATCTTCGGCGAAACGGCGGTCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGa             <  1:362927/70‑1 (MQ=255)                                                               aGCGCAGGTTTATCTTCGGCGAAACGGCGGTCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGAttt          <  1:2996953/71‑1 (MQ=255)                                                                gcgcAGGTTTATCTTCGGCGAAACGGCGGTCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGAtttt         >  1:6025800/1‑71 (MQ=255)                                                                  gcAGGTTTATCTTCGGCGAAACGGCGGTCAATTTc                                           >  1:6136145/1‑35 (MQ=255)                                                                  gcAGGTTTATCTTCGGCGAAACGGCGGTCAATTTc                                           >  1:6447451/1‑35 (MQ=255)                                                                  gcAGGTTTATCTTCGGCGAAACGGCGGTCAATTTc                                           >  1:3744480/1‑35 (MQ=255)                                                                  gcAGGTTTATCTTCGGCGAAACGGCGGTCAATTTc                                           >  1:3058089/1‑35 (MQ=255)                                                                  gcAGGTTTATCTTCGGCGAAACGGCGGTCAATTTc                                           >  1:3049843/1‑35 (MQ=255)                                                                  gcAGGTTTATCTTCGGCGAAACGGCGGTCAATTTc                                           >  1:2676214/1‑35 (MQ=255)                                                                  gcAGGTTTATCTTCGGCGAAACGGCGGTCAATTTc                                           >  1:1611016/1‑35 (MQ=255)                                                                   cAGGTTTATCTTCGGCGAAACGGCGGTCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGa             >  1:3591883/1‑64 (MQ=255)                                                                       tttATCTTCGGCGAAACGGCGGTCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGc  >  1:4938968/1‑71 (MQ=255)                                                                       tttATCTTCGGCGAAACGGCGGTCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGc  <  1:656367/71‑1 (MQ=255)                                                                       |                                                                       TGATTTCAGTTTTGTGGAAGATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTTGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGTCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGC  >  Exported/62414‑62554

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑