Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 2,736,272 T→C 5.0% R472R (CGT→CGC fusA → protein chain elongation factor EF‑G

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported2,736,2720TC 5.0% 615.8 / 16.7 199R472R (CGT→CGCfusAprotein chain elongation factor EF‑G
Reads supporting (aligned to +/- strand):  ref base T (84/105);  new base C (4/6);  total (88/111)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.26e-01

Only 100 of 203 total aligned reads displayed.

ACGAAGAATCTAACCAGACCATCATCGCGGGTATGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGTTGAAGCGAACGTAGGTAAACCGCAGGTTGCTTACCGTGAAACTATCCGC  >  Exported/2736205‑2736341
                                                                   |                                                                     
aCGAAGAATCTAACCAGACCATCATCGCGGGTATGGGCGAACTGCACCTCGACATCATCGTTGACCGTATg                                                                    <  1:3796661/71‑1 (MQ=255)
  gaagaaTCTAACCAGACCATCATCGCGGGTATGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGaa                                                                  <  1:6709180/71‑1 (MQ=255)
   aagaaTCTAACCAGACCATCATCGCGGGTATGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGaa                                                                  >  1:5876865/1‑70 (MQ=255)
   aagaaTCTAACCAGACCATCATCGCGGGTATGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAg                                                                 >  1:4082267/1‑71 (MQ=255)
   aagaaTCTAACCAGACCATCATCGCGGGTATGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAg                                                                 >  1:2238149/1‑71 (MQ=255)
       aTCTAACCAGACCATCATCGCGGGTATGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTg                                                             <  1:4693833/71‑1 (MQ=255)
              cAGACCATCATCGCGGGTATGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCaa                                                      <  1:6506416/71‑1 (MQ=255)
              cAGACCATCATCGCGGGTATGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCaa                                                      <  1:1388362/71‑1 (MQ=255)
              cAGACCATCATCGCGGGTATGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCaa                                                      <  1:4957974/71‑1 (MQ=255)
              cAGACCATCATCGCGGGTATGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCaa                                                      <  1:4937805/71‑1 (MQ=255)
              cAGACCATCATCGCGGGTATGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCaa                                                      <  1:2469836/71‑1 (MQ=255)
              cAGACCATCATCGCGGGTATGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCaa                                                      <  1:2226066/71‑1 (MQ=255)
                   catcatCGCGGGTATGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGtt                                                  >  1:6349278/1‑70 (MQ=255)
                   catcatCGCGGGTATGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGtt                                                  >  1:6341445/1‑70 (MQ=255)
                     tcatcGCGGGTATGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGTTg                                                 >  1:6543640/1‑69 (MQ=255)
                      catcGCGGGTATGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGTTGAAg                                              >  1:6841090/1‑71 (MQ=255)
                       atcGCGGGTATGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCaa                                                      >  1:101987/1‑62 (MQ=255)
                       atcGCGGGTATGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCaa                                                      >  1:4710064/1‑62 (MQ=255)
                       atcGCGGGTATGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCaa                                                      >  1:2731120/1‑62 (MQ=255)
                       atcGCGGGTATGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCaa                                                      >  1:549682/1‑62 (MQ=255)
                       atcGCGGGTATGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGTTGAAGc                                             >  1:1823228/1‑71 (MQ=255)
                       atcGCGGGTATGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGTTGAAGc                                             >  1:6907932/1‑71 (MQ=255)
                       atcGCGGGTATGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGTTGAAGc                                             >  1:426443/1‑71 (MQ=255)
                       atcGCGGGTATGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGTTGAAGc                                             >  1:1516395/1‑71 (MQ=255)
                       atcGCGGGTATGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGTTGAAGc                                             >  1:934541/1‑71 (MQ=255)
                        tcGCGGGTATGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGt                                                              <  1:4227830/53‑1 (MQ=255)
                        tcGCGGGTATGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGt                                                              <  1:3082801/53‑1 (MQ=255)
                        tcGCGGGTATGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGt                                                              <  1:661245/53‑1 (MQ=255)
                        tcGCGGGTATGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGTTg                                                 <  1:177773/66‑1 (MQ=255)
                          gcgGGTATGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGTTGAAGCGaa                                          >  1:4982906/1‑71 (MQ=255)
                          gcgGGTATGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGTTGAAGCGaa                                          >  1:5041131/1‑71 (MQ=255)
                          gcgGGTATGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGTTGAAGCGaa                                          >  1:3488603/1‑71 (MQ=255)
                          gcgGGTATGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGTTGAAGCGaa                                          >  1:5120762/1‑71 (MQ=255)
                          gcgGGTATGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGTTGAAGCGaa                                          >  1:242732/1‑71 (MQ=255)
                          gcgGGTATGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGTTGAAGCGa                                           <  1:4530414/70‑1 (MQ=255)
                              gTATGGGCGAACTGCACCTCGACATCATCGTTGACCGTAt                                                                     <  1:6473523/40‑1 (MQ=255)
                              gTATGGGCGAACTGCACCTCGACATCATCGTTGACCGTAt                                                                     <  1:5248998/40‑1 (MQ=255)
                               tATGGGCGAGCTGCACCTGGAAATTATCGTTGACCGCAt                                                                     <  1:1294041/39‑1 (MQ=16)
                               tATGGGCGAGCTGCACCTGGAAATTATCGTTGACCGCAt                                                                     <  1:1479535/39‑1 (MQ=16)
                               tATGGGCGAGCTGCACCTGGAAATTATCGTTGACCGCAt                                                                     <  1:4345253/39‑1 (MQ=16)
                               tATGGGCGAGCTGCACCTGGAAATTATCGTTGACCGCAt                                                                     <  1:2868414/39‑1 (MQ=16)
                               tATGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGCATTCaa                                                      <  1:887262/54‑1 (MQ=255)
                               tATGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCaa                                                      <  1:564299/54‑1 (MQ=255)
                               tATGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCaa                                                      <  1:589403/54‑1 (MQ=255)
                               tATGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCaa                                                      <  1:6637131/54‑1 (MQ=255)
                               tATGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCaa                                                      <  1:859970/54‑1 (MQ=255)
                               tATGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCaa                                                      <  1:3688840/54‑1 (MQ=255)
                                aTGGGCGAGCTGCACCTGGAAATTATCGTTGACCGCATGAAGCGTGAGTTCAACGTGGaa                                               >  1:5956996/1‑60 (MQ=255)
                                aTGGGCGAGCTGCACCTGGAAATTATCGTTGACCGCATGAAGCGTGAGTTCAACGTGGaa                                               >  1:372499/1‑60 (MQ=255)
                                aTGGGCGAGCTGCACCTGGAAATTATCGTTGACCGCATGAAGCGTGAGTTCAACGTGGaa                                               >  1:796882/1‑60 (MQ=255)
                                 tGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAAc                                                     <  1:4054594/53‑1 (MQ=255)
                                 tGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAAc                                                     <  1:4283245/53‑1 (MQ=255)
                                 tGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAAc                                                     <  1:761089/53‑1 (MQ=255)
                                 tGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGTTGAAGCGAACGTAgg                                    <  1:3339798/70‑1 (MQ=255)
                                 tGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGTTGAAGCGAACGTAgg                                    <  1:1969056/70‑1 (MQ=255)
                                 tGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGTTGAAGCGAACGTAgg                                    <  1:5683570/70‑1 (MQ=255)
                                 tGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGTTGAAGCGAACGTAgg                                    <  1:5715923/70‑1 (MQ=255)
                                 tGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGTTGAAGCGAACGTAgg                                    <  1:2295665/70‑1 (MQ=255)
                                 tGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGTTGAAGCGAACGTAgg                                    <  1:3127245/70‑1 (MQ=255)
                                 tGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGTTGAAGCGAACGTAgg                                    <  1:6114597/70‑1 (MQ=255)
                                 tGGGCGAACTGCACCTCAACATCATCGTTGACCGTATGAAGCGTGAATTCAACGTTGAAGCGAACGTAgg                                    >  1:392734/1‑70 (MQ=255)
                                  gggCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGTTGAATCGAACGTAgg                                    <  1:362588/69‑1 (MQ=255)
                                   ggCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGt                                                   >  1:1187896/1‑53 (MQ=255)
                                    gCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCaa                                                      >  1:4832772/1‑49 (MQ=255)
                                    gCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCaa                                                      >  1:5413812/1‑49 (MQ=255)
                                    gCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCaa                                                      >  1:4652862/1‑49 (MQ=255)
                                     cGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGTTGAAGCGAACGTAGGTAAAc                               >  1:6287149/1‑71 (MQ=255)
                                     cGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGTTGAAGCGAACGTAGGTAAAc                               >  1:622032/1‑71 (MQ=255)
                                     cGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGTTGAAGCGAACGTAGGTAAAc                               >  1:3319087/1‑71 (MQ=255)
                                      gAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGTTGAAg                                              >  1:4413281/1‑55 (MQ=255)
                                      gAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGTTGAAg                                              >  1:3053469/1‑55 (MQ=255)
                                      gAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGTTGAAGc                                             <  1:4104792/56‑1 (MQ=255)
                                        aCTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGTTGAAGCGAACGTAGGTAAACCGc                            <  1:6628493/71‑1 (MQ=255)
                                         cTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGTTGAAGCGAACGTAGGTAAAcc                              >  1:6183520/1‑68 (MQ=255)
                                          tGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGt                                                   <  1:4463348/46‑1 (MQ=255)
                                          tGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGt                                                   <  1:3453177/46‑1 (MQ=255)
                                          tGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGt                                                   <  1:1614313/46‑1 (MQ=255)
                                           gCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGTTGAAGCGAACGTAGGTAAACCGCAgg                         >  1:4607324/1‑71 (MQ=255)
                                            cACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGTTGAAGCGAACGTAGGTAAACCGCAGGt                        <  1:3285452/71‑1 (MQ=255)
                                            cACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGTTGAAGCGAACGTAGGTAAACCGCAGGt                        <  1:1335273/71‑1 (MQ=255)
                                              ccTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGTTGAAGCGAACGTAGGTAAACCGCAGGTTg                      <  1:3570995/71‑1 (MQ=255)
                                              ccTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGTTGAAGCGAACGTAGGTAAACCGCAGGTTg                      <  1:1888268/71‑1 (MQ=255)
                                                 cGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGTTGAAGCGAACGTAGGTaaa                                >  1:5331688/1‑58 (MQ=255)
                                                 cGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGTTGAAGCGAACGTAGGTaaa                                >  1:2638174/1‑58 (MQ=255)
                                                  gACATCATCGTTGACCGTATGAAGCGTGAATTCAACTTTGAAGCGAACGTAGGTAAACCGCAGGTTGCt                    <  1:4486772/69‑1 (MQ=255)
                                                  gACATCATCGTTGACCGTATGAAGCGTGAATTCAACGTTGAAGCGAACGTAGGTAAACCGCAGGTTGCt                    <  1:6065206/69‑1 (MQ=255)
                                                  gACATCATCGTTGACCGTATGAAGCGTGAATTCAACGTTGAAGCGAACGTAGGTAAACCGCAGGTTGCt                    <  1:1704167/69‑1 (MQ=255)
                                                  gACATCATCGTTGACCGTATGAAGCGTGAATTCAACGTTGAAGCGAACGTAGGTAAACCGCAGGTTGCTTa                  >  1:2967402/1‑71 (MQ=255)
                                                   aCATCATCGTTGACCGTATGAAGCGTGAATTCAACGTTGAAGCGAACGTAGGTAAACCGCAGGTTGCTTAc                 <  1:1770314/71‑1 (MQ=255)
                                                   aCATCATCGTTGACCGTATGAAGCGTGAATTCAACGTTGAAGCGAACGTAGGTAAACCGCAGGTTGCTTAc                 <  1:6954121/71‑1 (MQ=255)
                                                    catcatCGTTGACCGTATGAAGCGTGAATTCAACGTTGAAGCGAACGTAGGTAAACCGCAGGTTGCTTAcc                >  1:5454845/1‑71 (MQ=255)
                                                        atcGTTGACCGTATGAAGCGTGAATTCAACGTTGAAGCGAACGTAGGTaaa                                >  1:2340624/1‑51 (MQ=255)
                                                        atcGTTGACCGTATGAAGCGTGAATTCAACGTTGAAGCGAACGTAGGTAAACCGCAGGTTGCTTACCGTg             <  1:5177285/70‑1 (MQ=255)
                                                        atcGTTGACCGTATGAAGCGTGAATTCAACGTTGAAGCGAACGTAGGTAAACCGCAGGTTGCTTACCGTGa            <  1:6851514/71‑1 (MQ=255)
                                                        atcGTTGACCGTATGAAGCGTGAATTCAACGTTGAAGCGAACGTAGGTAAACCGCAGGTTGCTTACCGTGa            <  1:5140209/71‑1 (MQ=255)
                                                        atcGTTGACCGTATGAAGCGTGAATTCAACGTTGAAGCGAACGTAGGTAAACCGCAGGTTGCTTACCGTGa            <  1:3653874/71‑1 (MQ=255)
                                                           gTTGACCGTATGAAGCGTGAATTCAACGTTGAAGCGAACGTAGGTAAACCGCAGGTTGCTTACCGTGAAAc         <  1:2803320/71‑1 (MQ=255)
                                                             tGACCGTATGAAGCGTGAATTCAACGTTGAAGCGAACGTAGGTAAACCGCAGGTTGCTTACCGTGAAACTa       >  1:738622/1‑71 (MQ=255)
                                                                  gTATGAAGCGTGAATTCAACGTTGAAGCGAACGTAGGTAAACCGCAGGTTGCTTACCGTGAAACTATCCGc  <  1:4254356/71‑1 (MQ=255)
                                                                   tATGAAGCGTGAATTCAACGTTGAAGCGAACGTAGGTAAACCg                             <  1:3414116/43‑1 (MQ=255)
                                                                   |                                                                     
ACGAAGAATCTAACCAGACCATCATCGCGGGTATGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGTTGAAGCGAACGTAGGTAAACCGCAGGTTGCTTACCGTGAAACTATCCGC  >  Exported/2736205‑2736341

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: