| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 2,770,171 | G→A | 7.8% | V232M (GTG→ATG) | metA → | homoserine transsuccinylase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 2,770,171 | 0 | G | A | 7.8% | 374.4 / 16.0 | 115 | V232M (GTG→ATG) | metA | homoserine transsuccinylase |
| Reads supporting (aligned to +/- strand): ref base G (41/65); new base A (3/6); total (44/71) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 126 total aligned reads displayed.
GAAATTCTGGCAGAGACGGAAGAAGGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCATTGCCTTTGTGACGGGCCATCCCGAATATGATGCGCAAACGCTGGCGCAGGAATTTTTCCGCGATGTGGAAGCCG > Exported/2770102‑2770237 | gAAATTCTGGCAGAGACGGAAGAAGGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCATTGCCTTtgt < 1:6551740/71‑1 (MQ=255)gAAATTCTGGCAGAGACGGAAGAAGGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCATTGCCTTtgt < 1:2996505/71‑1 (MQ=255)gAAATTCTGGCAGAGACGGAAGAAGGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCATTGCCTTtgt < 1:4383452/71‑1 (MQ=255) aTTCTGGCAGAGACGGAAGAAGGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCATTGCCTTTGTGa > 1:1197690/1‑70 (MQ=255) aTTCTGGCAGAGACGGAAGAAGGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCATTGCCTTTGTGAc > 1:39068/1‑71 (MQ=255) cTGGCAGAGACGGAAGAAGGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCATTGCCTTTGTGACggg > 1:1406422/1‑71 (MQ=255) cTGGCAGAGACGGAAGAAGGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCATTGCCTTTGTGACggg > 1:4034681/1‑71 (MQ=255) cTGGCAGAGACGGAAGAAGGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCATTGCCTTTGTGACggg > 1:2365431/1‑71 (MQ=255) cAGAGACGGAAGAAGGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCATTGCCTTTGTGACGGGCCAt < 1:6288106/71‑1 (MQ=255) cAGAGACGGAAGAAGGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCATTGCCTTTGTGACGGGCCAt < 1:5136458/71‑1 (MQ=255) gagaCGGAAGAAGGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCATTGCCTTTATGACGGGCCATcc > 1:4684725/1‑71 (MQ=255) gagaCGGAAGAAGGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCATTGCCTTTATGACGGGCCATcc > 1:143912/1‑71 (MQ=255) gagaCGGAAGAAGGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCATTGCCTTTATGAAGGGCCATcc > 1:234891/1‑71 (MQ=255) gaCGGAAGAAGGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCATTGCCTTTGTGACGGGCCATccc > 1:5437613/1‑70 (MQ=255) gaCGGAAGAAGGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCATTGCCTTTGTGACGGGCCATCCCg < 1:6683107/71‑1 (MQ=255) cGGAAGAAGGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCATTGCCTTTATGACGGGCCATCCCGaa < 1:2051473/71‑1 (MQ=255) cGGAAGAAGGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCATTGCCTTTATGACGGGCCATCCCGaa < 1:627278/71‑1 (MQ=255) cGGAAGAAGGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCATTGCCTTTATGACGGGCCATCCCGaa < 1:4191426/71‑1 (MQ=255) cGGAAGAAGGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCATTGCCTTTATGACGGGCCATCCCGaa < 1:6323119/71‑1 (MQ=255) cGGAAGAAGGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCATTGCCTTTATGACGGGCCATCCCGaa < 1:2897041/71‑1 (MQ=255) aagaagGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCATTGCCTTTGTGACGGGCCATCCCGAatat < 1:3883278/71‑1 (MQ=255) gaagGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCATTGCCTTTGTGa > 1:3292907/1‑52 (MQ=255) gaagGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCATTGCCTTTGTGa > 1:6497578/1‑52 (MQ=255) aTGCATATCTGTTTGCCAGTAAAGATAAGCGCATTGCCTTTGTGACGGGCCATCCCGAATATGATGCGCa < 1:324859/70‑1 (MQ=255) aTGCATATCTGTTTGCCAGTAAAGATAAGCGCATTGCCTTTGTGACGGGCCATCCCGAATATGATGCGCa < 1:5487257/70‑1 (MQ=255) aTGCATATCTGTTTGCCAGTAAAGATAAGCGCATTGCCTTTGTGACGGGCCATCCCGAATATGATGCGCa < 1:3767451/70‑1 (MQ=255) aTGCATATCTGTTTGCCAGTAAAGATAAGCGCATTGCCTTTGTGACGGGCCATCCCGAATATGATGCGCa < 1:4692903/70‑1 (MQ=255) aTGCATATCTGTTTGCCAGTAAAGATAAGCGCATTGCCTTTGTGACGGGCCATCCCGAATATGATGCGCa < 1:4856765/70‑1 (MQ=255) aTGCATATCTGTTTGCCAGTAAAGATAAGCGCATTGCCTTTGTGACGGGCCATCCCGAATATGATGCGCa < 1:1459641/70‑1 (MQ=255) aTGCATATCTGTTTGCCAGTAAAGATAAGCGCATTGCCTTTGTGACGGGCCATCCCGAATATGATGCGCa < 1:1825430/70‑1 (MQ=255) tGCATATCTGTTTGCCAGTAAAGATAAGCGCATTGCCTTtgt < 1:4151750/42‑1 (MQ=255) tGCATATCTGTTTGCCAGTAAAGATAAGCGCATTGCCTTtgt < 1:3588321/42‑1 (MQ=255) tGCATATCTGTTTGCCAGTAAAGATAAGCGCATTGCCTTtgt < 1:6057637/42‑1 (MQ=255) atatCTGTTTGCCAGTAAAGATAAGCGCATTGCCTTTGTGACGGGCCATCCCGAATATGATGCGCAAACGc < 1:3513088/71‑1 (MQ=255) tatCTGTTTGCCAGTAAAGATAAGCGCATTGCCTTTGTGACGGGCCATCCCGaa < 1:709081/54‑1 (MQ=255) atCTGTTTGCCAGTAAAGATAAGCGCATTGCCTTTGTGACGGGCCATCCCGAATATGATGCGCAAACGCTg > 1:2098611/1‑71 (MQ=255) tCTGTTTGCCAGTAAAGATAAGCGCATTGCCTTTGTGACGGGCCATCCCGAATATGATGCGCAAACGCTgg > 1:3301488/1‑71 (MQ=255) tCTGTTTGCCAGTAAAGATAAGCGCATTGCCTTTGTGACGGGCCATCCCGAATATGATGCGCAAACGCTgg > 1:3764942/1‑71 (MQ=255) tCTGTTTGCCAGTAAAGATAAGCGCATTGCCTTTGTGACGGGCCATCCCGAATATGATGCGCAAACGCTg > 1:5007923/1‑70 (MQ=255) tGTTTGCCAGTAAAGATAAGCGCATTGCCTTTGTGACGGGCCATCCCGAatat < 1:3058806/53‑1 (MQ=255) tGTTTGCCAGTAAAGATAAGCGCATTGCCTTTGTGACGGGCCATCCCGAatat < 1:5040753/53‑1 (MQ=255) tGTTTGCCAGTAAAGATAAGCGCATTGCCTTTGTGACGGGCCATCCCGAatat < 1:2472235/53‑1 (MQ=255) tGTTTGCCAGTAAAGATAAGCGCATTGCCTTTGTGACGGGCCATCCCGAata > 1:6894712/1‑52 (MQ=255) tGTTTGCCAGTAAAGATAAGCGCATTGCCTTTGTGACGGGCCATCCCGAATatgatg > 1:239591/1‑57 (MQ=255) tGTTTGCCAGTAAAGATAAGCGCATTGCCTTTGTGACGGGCCATCCCGAATatgatg > 1:892481/1‑57 (MQ=255) tGTTTGCCAGTAAAGATAAGCGCATTGCCTTTGTGACGGGCCATCCCGAATatgatg > 1:6620755/1‑57 (MQ=255) tGTTTGCCAGTAAAGATAAGCGCATTGCCTTTGTGACGGGCCATCCCGAATatgatg > 1:231515/1‑57 (MQ=255) ttGCCAGTAAAGATAAGCGCATTGCCTTTGTGACGGGCCATCCCGAATATGATGCGCAAACg < 1:4099288/62‑1 (MQ=255) ccAGTAAAGATAAGCGCATTGCCTTTGTGACGGGCCATCCCGAATATGATGCGCAAACGCTGGCGCAGGa < 1:1402043/70‑1 (MQ=255) gTAAAGATTAGCGCATTGCCTTTGTGACGGGCCATCCCGaa < 1:6043999/41‑1 (MQ=255) gTAAAGATAAGCGCATTGCCTTTGTGACGGGCCATCCCGaa < 1:2005911/41‑1 (MQ=255) gTAAAGATAAGCGCATTGCCTTTGTGACGGGCCATCCCGaa < 1:3435822/41‑1 (MQ=255) gTAAAGATAAGCGCATTGCCTTTGTGACGGGCCATCCCGaa < 1:5861593/41‑1 (MQ=255) gTAAAGATAAGCGCATTGCCTTTGTGACGGGCCATCCCGaa < 1:872818/41‑1 (MQ=255) gTAAAGATAAGCGCATTGCCTTTGTGACGGGCCATCCCGaa < 1:1646569/41‑1 (MQ=255) gTAAAGATAAGCGCATTGCCTTTGTGACGGGCCATCCCGaa < 1:5896734/41‑1 (MQ=255) gTAAAGATAAGCGCATTGCCTTTGTGACGGGCCATCCCGaa < 1:4351783/41‑1 (MQ=255) tAAAGATAAGCGCATTGCCTTTGTGACGGGCCATCCCGAATATGATGCGCAAACGCTGGCGCAGGAAtt < 1:2747604/69‑1 (MQ=255) aaaGATAAGCGCATTGCCTTTGTGACGGGCCATCCCGAata > 1:1017601/1‑41 (MQ=255) aaaGATAAGCGCATTGCCTTTGTGACGGGCCATCCCGAATATGATGCGCAAACGCTGGCGCAgg < 1:4535212/64‑1 (MQ=255) aaaGATAAGCGCATTGCCTTTGTGACGGGCCATCCCGAATATGATGCGCAAACGCTGGCGCAgg < 1:4691611/64‑1 (MQ=255) aaaGATAAGCGCATTGCCTTTGTGACGGGCCATCCCGAATATGATGCGCAAACGCTGCCGCAgg < 1:2966852/64‑1 (MQ=255) aaGATAAGCGCATTGCCTTTGTGACGGGCCATCCCGAATatga > 1:961369/1‑43 (MQ=255) aaGATAAGCGCATTGCCTTTGTGACGGGCCATCCCGAATatga > 1:2473552/1‑43 (MQ=255) aaGATAAGCGCATTGCCTTTGTGACGGGCCATCCCGAATatga > 1:3773519/1‑43 (MQ=255) aaGATAAGCGCATTGCCTTTGTGACGGGCCATCCCGAATATGATGCGCAAACGCTGGCGCAGGAAtttt > 1:5122018/1‑69 (MQ=255) aaGATAAGCGCATTGCCTTTGTGACGGGCCATCCCGAATATGATGCGCAAACGCTGGCGCAGGAAt < 1:1805629/66‑1 (MQ=255) aaGATAAGCGCATTGCCTTTGTGACGGGCCATCCCGAATATGATGCGCAAACGCTGGCGCAGGAAt < 1:5770535/66‑1 (MQ=255) aaGATAAGCGCATTGCCTTTGTGACGGGCCATCCCGAATATGATGCGCAAACGCTGGCGCAGGAATTTTTc > 1:3540726/1‑71 (MQ=255) aGATAAGCGCATTGCCTTTGTGACGGGCCATCCCGAATatga > 1:790128/1‑42 (MQ=255) aGATAAGCGCATTGCCTTTGTGACGGGCCATCCCGAATATGATGCGCAAACGCTGGCGCAGGaa > 1:3123160/1‑64 (MQ=255) aGATAAGCGCATTGCCTTTGTGACGGGCCATCCCGAATATGATGCGCAAACGCTGGCGCAGGaa > 1:4829504/1‑64 (MQ=255) aTAAGCGCATTGCCTTTGTGACGGGCCATCCCGAatat < 1:4762514/38‑1 (MQ=255) aTAAGCGCATTGCCTTTGTGACGGGCCATCCCGAatat < 1:6097189/38‑1 (MQ=255) aTAAGCGCATTGCCTTTGTGACGGGCCATCCCGAatat < 1:5726143/38‑1 (MQ=255) aTAAGCGCATTGCCTTTGTGACGGGCCATCCCGAatat < 1:4665575/38‑1 (MQ=255) aTAAGCGCATTGCCTTTGTGACGGGCCATCCCGAatat < 1:4307120/38‑1 (MQ=255) aTAAGCGCATTGCCTTTGTGACGGGCCATCCCGAATATGATGCTCAAACGCTGGCGCAGGAATTTTTCcgc < 1:660704/71‑1 (MQ=255) gcATTGCCTTTGTGACGGGCCATCCCGAATATGATGCGCAAACGCTGGCGCAGGAATTTTTCCGCGAtgtg > 1:3962129/1‑71 (MQ=255) cATTGCCTTTGTGACGGGCCATCCCGAATATGATGCGCAAACGCTGGCGCAGGAATTTTTCCGCGATGTgg < 1:3605289/71‑1 (MQ=255) cATTGCCTTTGTGACGGGCCATCCCGAATATGATGCGCAAACGCTGGCGCAGGAATTTTTCCGCGATGTgg < 1:2054619/71‑1 (MQ=255) aTTGCCTTTGTGACGGGCCATCCCGAATATGATGCGCa < 1:6035500/38‑1 (MQ=255) aTTGCCTTTGTGACGGGCCATCCCGAATATGATGCGCa < 1:3865500/38‑1 (MQ=255) aTTGCCTTTGTGACGGGCCATCCCGAATATGATGCGCa < 1:1136336/38‑1 (MQ=255) aTTGCCTTTGTGACGGGCCATCCCGAATATGATGCGCa < 1:3200991/38‑1 (MQ=255) ttGCCTTTGTGACGGGCCATCCCGAATATGATGCGCAAACGCTGGCGCAGGAATTTTTCCGCGATGTGGa < 1:5359545/70‑1 (MQ=255) gCCTTTGTGACGGGCTATCCCGAATATGATGCGCAAACGCTGGCGCAGGAATTTTTCCGCGATGTGGAAg > 1:3613249/1‑70 (MQ=255) gCCTTTGTGACGGGCCATCCCGAATATGATGCGCAAACGCTg > 1:6488287/1‑42 (MQ=255) gCCTTTGTGACGGGCCATCCCGAATATGATGCGCAAACGCTg > 1:6311701/1‑42 (MQ=255) gCCTTTGTGACGGGCCATCCCGAATATGATGCGCAAACGCTGGCGCAGGAATTTTTCCGCGATGTGGAAg < 1:3184370/70‑1 (MQ=255) gCCTTTGTGACGGGCCATCCCGAATATGATGCGCAAACGCTGGCGCAGGAATTTTTCCGCGATGTGGAAg < 1:3586542/70‑1 (MQ=255) gCCTTTGTGACGGGCCATCCCGAATATGATGCGCAAACGCTGGCGCAGGAATTTTTCCGCGATGTGGAAg < 1:6949295/70‑1 (MQ=255) ccTTTGTGACGGGCCATCCCGAATATGATGCGCAAACGCTGGCGCAGGAAtttt > 1:6602435/1‑54 (MQ=255) cTTTGTGACGGGCCATCCCGAATATGATGCGCAAACGCTGGCGCAGGAATTTTTCCGCGATGTGGAAGCCg < 1:1689101/71‑1 (MQ=255) cTTTGTGACGGGCCATCCCGAATATGATGCGCAAACGCTGGCGCAGGAATTTTTCCGCGATGTGGAAGCCg < 1:3524196/71‑1 (MQ=255) cTTTGTGACGGGCCATCCCGAATATGATGCGCAAACGCTGGCGCAGGAATTTTTCCGCGATGTGGAAGCCg < 1:2739590/71‑1 (MQ=255) ttGTGACGGGCCATCCCGAATATGATGCGCAAACGCTGGCGCAGGAATTTTTCCGCGAtgtg < 1:3265007/62‑1 (MQ=255) tcgtgACGGGCCATCCCGAATATGATGCGCAAACGCTGGCGCAGGAATTTTTCCGCGAtgtg < 1:4859227/60‑1 (MQ=255) tgtgACGGGCCATCCCGAATATGATGCGCAAACGCTGGCGCAGGAATTTTTc > 1:6513688/1‑52 (MQ=255) tgtgACGGGCCATCCCGAATATGATGCGCAAACGCTGGCGCAGGAATTTTTc > 1:1134843/1‑52 (MQ=255) | GAAATTCTGGCAGAGACGGAAGAAGGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCATTGCCTTTGTGACGGGCCATCCCGAATATGATGCGCAAACGCTGGCGCAGGAATTTTTCCGCGATGTGGAAGCCG > Exported/2770102‑2770237 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |