Predicted mutation
evidence seq id position mutation freq annotation gene description
MC JC AP009048 2,176,983 Δ1,199 bp 100% [gatA][gatA] [gatA], insH, [gatA]

Missing coverage evidence...
   seq id start end size ←reads reads→ gene description
* * ÷ AP009048 2176983–2178180 2178181 2–1199 74 [0] [0] 72 [gatA]–[gatA] [gatA],insH,[gatA]

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? AP009048 = 21769820 (0.000)53 (1.000) 38/92 NT 100% coding (71/321 nt) gatA galactitol‑specific enzyme IIA component of PTS
?AP009048 2178182 = 0 (0.000)coding (202/315 nt) gatA galactitol‑specific enzyme IIA component of PTS

TCGTTATCATCATCCGCTTGCTGAAAATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  AP009048/2176910‑2176982
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑cttagCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCATTATCCCGGTAGGGAATTCTG  >  AP009048/2178182‑2178250
                                                                                                                                              
TCGTTATCATCATCCGCTTGCTGAAAATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACT                                                                          >  1:5221163/1‑70
  GTTATCATCATCCGCTTGCTGAAAATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAG                                                                       <  1:1163110/71‑1
  GTTATCATCATCCGCTTGCTGAAAATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAG                                                                       <  1:3472537/71‑1
   TTATCATCATCCGCTTGCTGAAAATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGC                                                                      <  1:2205771/71‑1
   TTATCATCATCCGCTTGCTGAAAATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGC                                                                      <  1:1720203/71‑1
   TTATCATCATCAGCTTGCTGAAAATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTA                                                                        >  1:4177345/1‑69
      TCATCATCCGCTTGCTGAAAATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCAT                                                                    <  1:4825815/70‑1
        ATCATCCGCTTGCTGAAAATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGA                                                                  <  1:528718/70‑1
         TCATCCGCTTGCTGAAAATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCAT                                                                    >  1:1985882/1‑67
              CGCTTGCTGAAAATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCC                                                            <  1:2223049/70‑1
                 TTGCTGAAAATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAG                                                                       <  1:3351040/56‑1
                 TTGCTGAAAATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAG                                                                       <  1:2944365/56‑1
                    CTGAAAATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGAC                                                                           <  1:2153369/49‑1
                       AAAATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGC                                                   <  1:3413767/70‑1
                       AAAATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAAT                                                                >  1:5164271/1‑57
                        AAATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGG                                                 <  1:3587841/71‑1
                        AAATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGG                                                 <  1:1266778/71‑1
                         AATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGG                                                 >  1:2232665/1‑70
                         AATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGG                                                 >  1:2570967/1‑70
                          ATGAACTTTATTTGTTAGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTA                                               >  1:2325365/1‑71
                            GAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGG                                                 <  1:159271/67‑1
                             AACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTG                                            >  1:69616/1‑71
                             AACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATG                                                    <  1:1935139/63‑1
                                TTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATG                                                    >  1:5227385/1‑60
                                  TATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAA                                         >  1:1222302/1‑69
                                  TATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAA                                         >  1:1996452/1‑69
                                  TATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAA                                         <  1:3568930/69‑1
                                  TATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGC                                                   >  1:943319/1‑59
                                   ATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATA                                       <  1:3233110/70‑1
                                     TTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCG                                    >  1:823406/1‑71
                                     TTGTTGGCCTTAACAGATAAATGGCTGACGACTTAG                                                                       <  1:3938462/36‑1
                                      TGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCG                                    <  1:2350968/70‑1
                                       GTTGGCCTGAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAA                                         <  1:4882291/64‑1
                                        TTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGC                                 <  1:3932819/71‑1
                                        TTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGC                                 <  1:3926314/71‑1
                                          GGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTG                               >  1:4654197/1‑71
                                          GGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCT                                >  1:5149482/1‑70
                                          GGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCT                                <  1:1837574/70‑1
                                             CTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTC                            <  1:3216452/71‑1
                                             CTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTC                            <  1:4728444/71‑1
                                             CTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTC                            <  1:1627768/71‑1
                                             CTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCT                             >  1:1337695/1‑70
                                              TTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTC                            >  1:3297211/1‑70
                                               TAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTC                            >  1:1354946/1‑69
                                               TAAAAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCT                                >  1:1811531/1‑65
                                                AACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAG                         >  1:911315/1‑71
                                                 ACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAG                         <  1:3763721/70‑1
                                                 ACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAA                          <  1:3764280/69‑1
                                                 ACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAA                          >  1:4557944/1‑69
                                                 ACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGT                                                <  1:5033512/47‑1
                                                  CAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATG                                                    >  1:3710073/1‑42
                                                   AGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCAT                      <  1:1820246/71‑1
                                                    GATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCAT                      <  1:3308484/70‑1
                                                    GATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTA                                               <  1:3805653/45‑1
                                                     ATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCATTA                    >  1:3369548/1‑71
                                                      TAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCATTAT                   <  1:954019/71‑1
                                                      TAAATGGCTGACGACTTAGCATGAATCGCCTCACAA                                                      >  1:4204290/1‑36
                                                       AAATGTCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCATTA                    >  1:4534944/1‑69
                                                        AATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCATTATCC                 <  1:4816222/71‑1
                                                        AATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCATTATCC                 <  1:2890105/71‑1
                                                        AATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCATTATC                  <  1:2518114/70‑1
                                                            GCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCATTATCCCGG              <  1:2023436/70‑1
                                                            GCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGC                                 >  1:4386365/1‑51
                                                             CTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCATTATCCCGGTA            <  1:2778438/71‑1
                                                             CTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCATTATCCCGGTA            <  1:3911750/71‑1
                                                              TGACGACTTAGCATGAATCGCCTCACAATGCGGTA                                               <  1:3289841/35‑1
                                                                ACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCATTATCCCGGTAGGG         <  1:5064628/71‑1
                                                                ACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCATTATCCCGGTAGG          >  1:3981512/1‑70
                                                                ACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCATTATCCCGGTAGG          <  1:5287600/70‑1
                                                                ACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCATTATCCCGGTAG           >  1:565948/1‑69
                                                                 CGACTTAGCATGAATCGCCTCACAATGCGGTAATGCAATAGCGTGCTGCTC                            <  1:1651203/51‑1
                                                                   ACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCATTATCCCGGTAGGGAAT      <  1:2905693/71‑1
                                                                    CTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCAT                      >  1:2457347/1‑54
                                                                     TTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCATTATCCC                <  1:2553939/59‑1
                                                                       AGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCATTATCCCGGTAGGGAATTCTG  >  1:1161264/1‑71
                                                                       AGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCATTATCCCGGTAGGGAATTC    <  1:3954554/69‑1
                                                                       AGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCATTATCCCGGTAGGGAATT     <  1:2230850/68‑1
                                                                                                                                              
TCGTTATCATCATCCGCTTGCTGAAAATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  AP009048/2176910‑2176982
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑cttagCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCATTATCCCGGTAGGGAATTCTG  >  AP009048/2178182‑2178250

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.