Predicted mutation
evidence seq id position mutation freq annotation gene description
RA AP009048 3,684,687 A→G 100% pseudogene (372/582 nt)
pseudogene (1435/1645 nt)
ilvG ←
ilvG ←
ECK3760:JW3741:b3768; acetolactate synthase II, large subunit, C‑ter fragment
ECK3760:JW3740+JW3741:b4488; large subunit of acetolactate synthase II

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP0090483,684,6870AG100.0% 362.6 / NA 105pseudogene (372/582 nt)
pseudogene (1435/1645 nt)
ilvG
ilvG
ECK3760:JW3741:b3768; acetolactate synthase II, large subunit, C‑ter fragment
ECK3760:JW3740+JW3741:b4488; large subunit of acetolactate synthase II
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (49/56);  total (49/56)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 105 total aligned reads displayed.

TCTTTCCGGGTGATGTGTTGGCCATGGATGCCGAAGGCGCTGGCTAACATGAGGAAATCGGGGTTATCAGTAAGGGTGGTTTCGCTGTATCGTTCCTGAAAAAACAGTTGCTGCCATTGTCGAACCATCCCTAACC  >  AP009048/3684619‑3684754
                                                                    |                                                                   
tCTTTACGGGTGATGTGTTGGCCAGGGATGCCGAAGGCGCTGGCTAACATGAGGAAATCGGGGTTATCGGt                                                                   <  1:4896216/71‑1 (MQ=255)
    tacGGGTGATGTGTTGGCCAGGGATGCCGAAGGCGCTGGCTAACATGAGGAAATCGGGGTTATCGGTAAgg                                                               >  1:2721851/3‑71 (MQ=255)
    tacGGGTGATGTGTTGGCCAGGGATGCCGAAGGCGCTGGCTAACATGAGGAAATCGGGGTTATCGGTAAgg                                                               >  1:3326281/3‑71 (MQ=255)
    tacGGGTGATGTGTTGGCCAGGGATGCCGAAGGCGCTGGCTAAAATGAGGAAATCGGGGTTATCGGTAAgg                                                               >  1:2176572/3‑71 (MQ=255)
      cGGGTGATGTGTTGGCCAGGGATGCCGAAGGCGCTGGCTAACATGAGGAAATCGGGGTTATCGGTAAggg                                                              <  1:4198817/70‑1 (MQ=255)
      cGGGTGATGTGTTGGCCAGGGATGCCGAAGGCGCTGGCTAACATGAGGAAATCGGGGTTATCGGTAAGggt                                                             <  1:33538/71‑1 (MQ=255)
         gTGATGTGTTGGCCAGGGATGCCGAAGGCGCTGGCTAACATGAGGAAATCGGGGTTATCGGTAAGggtggt                                                          >  1:183365/1‑71 (MQ=255)
         gTGATGTGTTGGCCAGGGATGCCGAAGGCGCTGGCTAACATGAGGAAATCGGGGTTATCGGTAAGggtggt                                                          <  1:1454763/71‑1 (MQ=255)
          tGATGTGTTGGCCAGGGATGCCGAAGGCGCTGGCTAACATGAGGAAATCGGGGTTATCGGTAAgg                                                               <  1:1952237/65‑1 (MQ=255)
          tGATGTGTTGGCCAGGGATGCCGAAGGCGCTGGCTAACATGAGGAAATCGGGGTTATCGGTAAGGGTGGtt                                                         <  1:1573023/71‑1 (MQ=255)
          tGATGTGTTGGCCAGGGATGCCGAAGGCGCTGGCTAACATGAGGAAATCGGGGTTATCGGTAAGGGTGGtt                                                         <  1:4719769/71‑1 (MQ=255)
            aTGTGTTGGCCAGGGATGCCGAAGGCGCTGGCTAACATGAGGAAATCGGGGTTATCGGTAAGGGTGGttt                                                        <  1:2699257/70‑1 (MQ=255)
             tgtgTTGGCCAGGGATGCCGAAGGCGCTGGCTAACATGAGGAAATCGGGGTTATCGGTAAGGGTGGTTTCg                                                      <  1:15178/71‑1 (MQ=255)
              gtgtTGGCCAGGGATGCCGAAGGCGCTGGCTAACATGAGGAAATCGGGGTTATCGGTAAGGGTGGTTTCGc                                                     >  1:4717627/1‑71 (MQ=255)
               tgtTGGCCAGGGATGCCGAAGGCGCTGGCTAACATGAGGAAATCGGGGTTATCGGTAAGggtg                                                            <  1:1056068/63‑1 (MQ=255)
               tgtTGGCCAGGGATGCCGAAGGCGCTGGCTAACATGAGGAAATCGGGGTTATCGGTAAGGGTGGTTTCGc                                                     >  1:3062302/1‑70 (MQ=255)
                 ttGGCCAGGGATGCCGAAGGCGCTGGCTAACATGAGGAAATCGGGGTTATCGGTAAGGGTGGTTTCGCtat                                                  <  1:3448529/71‑3 (MQ=255)
                 ttGGCCAGGGATGCCGAAGGCGCTGGCTAACATGAGGAAATCGGGGTTATCGGTAAGGGTGGTTTCGCtat                                                  <  1:4467747/71‑3 (MQ=255)
                 ttGGCCAGGGATGCCGAAGGCGCTGGCTAACATGAGGAAATCGGGGTTATCGGTAAGGGTGGTTTCGCtat                                                  <  1:4914016/71‑3 (MQ=255)
                 ttGGCCAGGGATGCCGAAGGCGCTGGCTAACATGAGGAAATCGGGGTTATCGGTAAGGGTGGTTTCGCta                                                   >  1:4253064/1‑69 (MQ=255)
                 ttGGCCAGGGATGCCGAAGGCGCTGGCTAACATGAGGAAATCGGGGTTATCGGTAAGGGTGGTTTCGCta                                                   >  1:763049/1‑69 (MQ=255)
                  tGGCCAGGGATGCCGAAGGCGCTGGCTAACATGAGGAAATCGGGGTTATCGGTAAGGGTGGTTTCGCtata                                                 >  1:1964874/1‑71 (MQ=255)
                   ggCCAGGGATGCCGAAGGCGCTGGCTAACATGAGGAAATCGGGGTTATCGGt                                                                   <  1:4089779/52‑1 (MQ=39)
                   ggCCAGGGATGCCGAAGGCGCTGGCTAACATGAGGAAATCGGGGTTATCGGTa                                                                  >  1:1544473/1‑53 (MQ=39)
                   ggCCAGGGATGCCGAAGGCGCTGGCTAACATGAGGAAATCGGGGTTATCGGTAAGGGTGGTTTCGCTatat                                                >  1:5209937/1‑71 (MQ=255)
                   ggCCAGGGATGCCGAAGGCGCTGGCTAACATGAGGAAATCGGGGTTATCGGTAAGGGTGGTTTCGCTatat                                                >  1:2334641/1‑71 (MQ=255)
                    gCCAGGGATGCCGAAGGCGCTGGCTAACATGAGGAAATCGGGGTTATCGGTAAgg                                                               <  1:3752105/55‑1 (MQ=255)
                    gCCAGGGATGCCGAAGGCGCTGGCTAACATGAGGAAATCGGGGTTATCGGTAAGGGTGGTTTCGCTATATc                                               <  1:4869511/71‑1 (MQ=255)
                     ccAGGGATGCCGAAGGCGCTGGCTAACATGAGGAAATCGGGGTTATCGGTaa                                                                 >  1:2633316/1‑52 (MQ=39)
                     ccAGGGATGCCGAAGGCGCTGGCTAACATGAGGAAATCGGGGTTATCGGTAAGGGTGGTTTCGCTATATCg                                              <  1:4500037/71‑1 (MQ=255)
                     ccAGGGATGCCGAAGGCGCTGGCTAACATGAGGAAATCGGGGTTATCGGTAAGGGTGGTTTCGCTATATCg                                              <  1:4317324/71‑1 (MQ=255)
                      cagggATGCCGAAGGCGCTGGCTAACATGAGGAAATCGGGGTTATCGGTAAGGGTGGTTTCGCTATATCGt                                             <  1:63377/68‑1 (MQ=255)
                      cagggATGCCGAAGGCGCTGGCTAACATGAGGAAATCGGGGTTATCGGTAAGGGTGGTTTCGCTATATCGt                                             <  1:1573001/68‑1 (MQ=255)
                       agggATGCCGAAGGCGCTGGCTAACATGAGGAAATCGGGGTTATCGGTAAGGGTGGTTTCGCTATATCGtt                                            >  1:435409/3‑71 (MQ=255)
                       agggATGCCGAAGGCGCTGGCTAACATGAGGAAATCGGGGTTATCGGTAAGGGTGGTTTCGCTATATCGtt                                            >  1:3015762/3‑71 (MQ=255)
                       agggATGCCGAAGGCGCTGGCTAACATGAGGAAATCGGGGTTATCGGTAAGGGTGGTTTCGCTATATCGtt                                            >  1:208560/3‑71 (MQ=255)
                        gggATGCCGAAGGCGCTGGCTAACATGAGGAAATCGGGGTTATCGGTAAGGGTGGTTTCGCTATATCGTTc                                           <  1:3774664/70‑1 (MQ=255)
                        gggATGCCGAAGGCGCTGGCTAACATGAGGAAATCGGGGTTATCGGTAAGGGTGGTTTCGCTATATCGTTc                                           >  1:3657762/2‑71 (MQ=255)
                           aTGCCGAAGGCGCTGGCTAACATGAGGAAATCGGGGTTATCGGTaa                                                                 >  1:3908589/1‑46 (MQ=255)
                           aTGCCGAAGGCGCTGGCTAACATGAGGAAATCGGGGTTATCGGTAAGGGTGGTTTCGCt                                                    <  1:5022311/59‑1 (MQ=255)
                           aTGCCGAAGGCGCTGGCTAACATGAGGAAATCGGGGTTATCGGTAAGGGTGGTTTCGCTATATCGTTCCt                                         >  1:2043647/1‑70 (MQ=255)
                            tGCCGAAGGCGCTGGCTAACATGAGGAAATCGGGGTTATCGGTAAGGGTGGTTTCGCTATATCGTTCCTgg                                       <  1:2435655/71‑2 (MQ=255)
                            tGCCGAAGGCGCTGGCTAACATGAGGAAATCGGGGTTATCGGTAAGGGTGGTTTCGCTATATCGTTCCTg                                        >  1:1197049/1‑70 (MQ=255)
                            tGCCGAAGGCGCTGGCTAACATGAGGAAATCGGGGTTATCGGTAAGGGTGGTTTCGCTATATCGTTCCTg                                        >  1:3658039/1‑70 (MQ=255)
                              ccGAAGGCGCTGGCTAACATGAGGAAATCGGGGTTATCGGTAAGGGTGGTTTCGCTATATCGTTCCt                                         >  1:3443302/1‑67 (MQ=255)
                               cGAAGGCGCTGGCTAACATGAGGAAATCGGGGTTATCGGTAAgg                                                               >  1:2895690/1‑44 (MQ=255)
                               cGAAGGCGCTGGCTAACATGAGGAAATCGGGGTTATCGGTAAGGGTGGTTTCGCTATATCGTTCCTGgaaa                                    <  1:3156036/71‑1 (MQ=255)
                                gAAGGCGCTGGCTAACATGAGGAAATCGGGGTTATCGGTAAGGGTGGTTTCGCt                                                    <  1:1351040/54‑1 (MQ=255)
                                gAAGGCGCTGGCTAACATGAGGAAATCGGGGTTATCGGTAAGGGTGGTTTCGCTATATCGTTCCTGgaaa                                    >  1:2884591/1‑70 (MQ=255)
                                 aaGGCGCTGGCTAACATGAGGAAATCGGGGTTATCGGTAAGGGTGGTTTCGCTATATCGTTCCTGgaaaaa                                  >  1:4615428/1‑71 (MQ=255)
                                 aaGGCGCTGGCTAACATGAGGAAATCGGGGTTATCGGTAAGGGTGGTTTCGCTATATCGTTCCTGgaaaaa                                  >  1:1119941/1‑71 (MQ=255)
                                   ggCGCTGGCTAACATGAGGAAATCGGGGTTATCGGTAAGGGTGGTTTCGCTATATCGTTcc                                          <  1:4314935/61‑1 (MQ=255)
                                    gcgcTGGCTAACATGAGGAAATCGGGGTTATCGGTAAGGGTGGTTTCGCTATATCGTTCCTGGAAAAACAg                               <  1:2823312/71‑1 (MQ=255)
                                    gcgcTGGCTAACATGAGGAAATCGGGGTTATCGGTAAGGGTGGTTTCGCTATATCGTTCCTGGAAAAACAg                               <  1:1793399/71‑1 (MQ=255)
                                     cgcTGGCTAACATGAGGAAATCGGGGTTATCGGTAAGGGTGGTTTCGCTATATCGTTCCTGGAAAAACAGt                              <  1:72082/71‑1 (MQ=255)
                                       cTGGCTAACATGAGGAAATCGGGGTTATCGGTAAGGGTGGTTTCGCTATATCGTTCCTGGAAAAACAGTtg                            <  1:5138101/71‑1 (MQ=255)
                                        tGGCTAACATGAGGAAATCGGGGTTATCGGTAAGGGTGGTTTCGCTATATCGTTCCTGGAAAAACAGTtgc                           >  1:5197318/1‑71 (MQ=255)
                                          gCTAACATGAGGAAATCGGGGTTATCGGTAAGggtg                                                            <  1:1324846/36‑1 (MQ=255)
                                          gCTAACATGAGGAAATCGGGGTTATCGGTAAGGGTGGTTTCGCTATATCGTTCCTGGAAAAACAGTtgctg                         >  1:4544153/1‑71 (MQ=255)
                                            tAACATGAGGAAATCGGGGTTATCGGTAAGGGTGGttt                                                        <  1:2179218/38‑1 (MQ=255)
                                            tAACATGAGGAAATCGGGGTTATCGGTAAGGGTGGTTTCGCTATATCGtt                                            >  1:1459330/1‑50 (MQ=255)
                                            tAACATGAGGAAATCGGGGTTATCGGTAAGGGTGGTTTCGCTATATCGTTCCTGGAAAAACAGtt                             >  1:4101208/1‑65 (MQ=255)
                                             aaCATGAGGAAATCGGGGTTATCGGTAAGGGTGGTTTCGCTATATCGTTCCTGGAAAAAc                                 <  1:3773027/60‑1 (MQ=255)
                                             aaCATGAGGAAATCGGGGTTATCGGTAAGGGTGGTTTCGCTATATCGTTCCTGGAAAAACAGt                              <  1:669714/63‑1 (MQ=255)
                                             aaCATGAGGAAATCGGGGTTATCGGTAAGGGTGGTTTCGCTATATCGTTCCTGGAAAAACAGTtgctg                         >  1:419031/1‑68 (MQ=255)
                                              aCATGAGGAAATCGGGGTTATCGGTAAGGGTGGTTTCGCta                                                   >  1:2519180/1‑40 (MQ=39)
                                              aCATGAGGAAATCGGGGTTATCGGTAAGGGTGGTTTCGCt                                                    >  1:1675210/1‑40 (MQ=255)
                                              aCATGAGGAAATCGGGGTTATCGGTAAGGGTGGTTTCGCTATATCGTTCCTGGAAAAACAGtt                             <  1:107172/63‑1 (MQ=255)
                                              aCATGAGGAAATCGGGGTTATCGGTAAGGGTGGTTTCGCTATATCGTTCCTGGAAAAACAGTTGCTGCCa                      <  1:3088751/70‑1 (MQ=255)
                                              aCATGAGGAAATCGGGGTTATCGGTAAGGGTGGTTTCGCTATATCGTTCCTGGAAAAACAGTTGCTGCCAt                     >  1:3785174/1‑71 (MQ=255)
                                              aCATGAGGAAATCGGGGTTATCGGTAAGGGTGGTTTCGCTATATCGTTCCTGGAAAAACAGTTGCTGCCAt                     >  1:4272919/1‑71 (MQ=255)
                                              aCATGAGGAAATCGGGGTTATCGGTAAGGGTGGTTTCGCTATATAGTTCCTGGAAAAACAGTTGCTGCCa                      <  1:856777/70‑1 (MQ=255)
                                               cATGAGGAAATCGGGGTTATCGGTAAGGGTGGTTTCGCTATATCGTTcc                                          <  1:378408/49‑1 (MQ=255)
                                               cATGAGGAAATCGGGGTTATCGGTAAGGGTGGTTTCGCTATATCGTTCCTGGAAAAACAGTTGCTGCCa                      <  1:2525663/69‑1 (MQ=255)
                                               cATGAGGAAATCGGGGTTATCGGTAAGGGTGGTTTCGCTATATCGTTCCTGGAAAAACAGTTGCTGCCAtt                    <  1:865326/71‑1 (MQ=255)
                                                aTGAGGAAATCGGGGTTATCGGTAAGGGTGGTTTCGCTATATCGTTCCTGGAAAAACAGTTGCTGCCATTg                   >  1:1631395/1‑71 (MQ=255)
                                                aTGAGGAAATCGGGGTTATCGGTAAGGGTGGTTTCGCTATATCGTTCCTGGAAAAACAGTTGCTGCCATTg                   <  1:2564019/71‑1 (MQ=255)
                                                aTGAGGAAATCGGGGTTATAGGTAAGGGTCGTTTCGatatat                                                >  1:5373604/1‑42 (MQ=19)
                                                 tGAGGAAATCGGGGTTATCGGTAAGGGTGGTTTCGCTATATCGt                                             >  1:1467702/1‑44 (MQ=38)
                                                  gAGGAAATCGGGGTTATCGGTAAGGGTGGTTTCGCTATATCGTTCCTGgaaaaa                                  >  1:2280790/1‑54 (MQ=255)
                                                  gAGGAAATCGGGGTTATCGGTAAGGGTGGTTTCGCTATATCGTTCCTGGAAAAACAGTtgc                           <  1:4252661/61‑1 (MQ=255)
                                                  gAGGAAATCGGGGTTATCGGTAAGGGTGGTTTCGCTATAACGTTCCTGgaaaa                                   >  1:3477294/1‑53 (MQ=255)
                                                   aGGAAATCGGGGTTATCGGTAAGGGTGGTTTCGCTATATCGTTcc                                          <  1:3677570/45‑1 (MQ=255)
                                                   aGGAAATCGGGGTTATCGGTAAGGGTGGTTTCGCTATATCGTTCCTGGAAAAACAGTTGCTGCCATTGTCg                <  1:4801936/71‑1 (MQ=255)
                                                   aGGAAATCGGGGTTATCGGTAAGGGTGGTTTCGCTATATCGTTCCTGGAAAAACAGTTGCTGCCATTGTCg                <  1:820412/71‑1 (MQ=255)
                                                    ggAAATCGGGGTTATCGGTAAGGGTGGTTTCGCTATATCGTTCCTGGAAAAACAGTTGCTGCCATTGTCg                <  1:1479771/70‑1 (MQ=255)
                                                    ggAAATCGGGGTTATCGGTAAGGGTGGTTTCGCTATATCGTTCCTGGAAAAACAGTTGCTGCCATTGTCg                >  1:3200681/1‑70 (MQ=255)
                                                       aaTCGGGGTTATCGGTAAGGGTGGTTTCGCTATATCGTTCCTGGAAAAACAGTTGCTGCCATTGTCGAAcc            <  1:378048/71‑1 (MQ=255)
                                                         tCGGGGTTATCGGTAAGGGTGGTTTCGCTATATCGt                                             <  1:4426516/36‑1 (MQ=38)
                                                         tCGGGGTTATCGGTAAGGGTGGTTTCGCTATATCGTTCCTGGAAAAACAGTTACTGCCATTGTCGAACCAt          <  1:4864395/71‑1 (MQ=255)
                                                          cGGGGTTATCGGTAAGGGTGGTTTCGCTATATCGTTCCTGGAAAAACAGTTGCTGCCATTGTCGAACCATc         >  1:3879371/1‑71 (MQ=255)
                                                            gggTTATCGGTAAGGGTGGTTTCGCTATATCGTTCCTGGAAAAACAGTTGCTGCCATTGTCGAACCATcc        >  1:38688/1‑70 (MQ=255)
                                                             ggTTATCGGTAAGGGTGGTTTCGCTATATCGTTCCTGGAAAAACAGTTGCTGCCATTGTCGAACCATCCCt      <  1:4541098/71‑1 (MQ=255)
                                                             ggTTATCGGTAAGGGTGGTTTCGCTATATCGTTCCTGGAAAAACAGTTGCTGCCATTGTCGAACCATCCCt      <  1:735323/71‑1 (MQ=255)
                                                               ttATCGGTAAGGGTGGTTTCGCTATATCGTTCCTGgaaaaa                                  <  1:1900458/41‑1 (MQ=255)
                                                               ttATCGGTAAGGGTGGTTTCGCTATATCGTTCCTGgaaaaa                                  <  1:414901/41‑1 (MQ=255)
                                                               ttATCGGTAAGGGTGGTTTCGCTATATCGTTCCTGgaaaa                                   <  1:5034384/40‑1 (MQ=255)
                                                               ttATCGGTAAGGGTGGTTTCGCTATATCGTTCCTGGAAAAACAGTtgct                          >  1:4563491/1‑49 (MQ=38)
                                                                tATCGGTAAGGGTGGTTTCGCTATATCGTTCCTGGAAAAACAGTTGCTGCCATTGTCGAACCATCCCTAAc   >  1:176369/1‑71 (MQ=255)
                                                                 aTCGGTAAGGGTGGTTTCGCTATATCGTTCCTGGAAAAACAGTTGCTGCCATTGTCGAACCATCCCTAAcc  >  1:4875002/1‑71 (MQ=255)
                                                                    |                                                                   
TCTTTCCGGGTGATGTGTTGGCCATGGATGCCGAAGGCGCTGGCTAACATGAGGAAATCGGGGTTATCAGTAAGGGTGGTTTCGCTGTATCGTTCCTGAAAAAACAGTTGCTGCCATTGTCGAACCATCCCTAACC  >  AP009048/3684619‑3684754

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: