| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | AP009048 | 3,684,885 | T→C | 100% | pseudogene (174/582 nt) pseudogene (1237/1645 nt) |
ilvG ← ilvG ← |
ECK3760:JW3741:b3768; acetolactate synthase II, large subunit, C‑ter fragment ECK3760:JW3740+JW3741:b4488; large subunit of acetolactate synthase II |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AP009048 | 3,684,885 | 0 | T | C | 100.0% | 89.7 / NA | 27 | pseudogene (174/582 nt) pseudogene (1237/1645 nt) | ilvG ilvG | ECK3760:JW3741:b3768; acetolactate synthase II, large subunit, C‑ter fragment ECK3760:JW3740+JW3741:b4488; large subunit of acetolactate synthase II |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (11/16); total (11/16) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GCACATTCATCATGAAAGAGCCGTCACCGGAGATACAGACAACGGTATCGTTCGGTCGCGCGACTTGTGCGCCAACCGCCGCCGGTAAACCAAAACCCATGGTACCTAAACCGCTGGAGGTGATGAAATTTTCC > AP009048/3684818‑3684951 | gCACATTCATCATGAAAGAGCCGTCACCGGAGATACAGACGACGGTATCGTTTGGTCGCGCGACTTgcgc > 1:3213881/1‑70 (MQ=255)gCACATTCATCATGAAAGAGCCGTCACCGGAGATACAGACGACGGTATCGTTTGGTCGCGCGACTTGcgcg > 1:3289431/1‑71 (MQ=255) caTTCATCATGAAAGAGCCGTCACCGGAGATACAGACGACGGTATCGTTTGGTCGCGCGACTTgcgcgc > 1:522398/1‑69 (MQ=255) ccGTCACCGGAGATACAGACGACGGTATCGTTTGGTCGCGCGACTTGCGCGCCAACCGCCGCCgg < 1:2870956/65‑1 (MQ=255) cGTCACCGGAGATACAGACGACGGTATCGTTTGGTCGCGCGACTTGCGCGCCAAccgcc < 1:609841/59‑1 (MQ=255) cACCGGAGATACAGACGACGGTATCGTTTGGTCGCGCGACTTGCGCGCCAACCGCCGCCGGTAAACCaaaa > 1:4293296/1‑71 (MQ=255) aCCGGAGATACAGACGACGGTATCGTTTGGTCGCGCGACTTGCGCGCCAACCGCCGCCGGTAAACCAAAAc > 1:699388/1‑71 (MQ=255) ccGGAGATACAGACGACGGTATCGTTTGGTCGCGCGACTTGCGCGCCAACCGCCGCCGGTAAACCAAAAcc > 1:1242849/1‑71 (MQ=255) cGGAGATACAGACGACGGTATCGTTTGGTCGCGCGACTTGCGCGCCAACCGCCGCCGGTAAACCAAAAcc > 1:1979605/1‑70 (MQ=255) gaTACAGACGACGGTATCGTTTGGTCGCGCGACTTGCGCGcc < 1:802628/42‑1 (MQ=37) tACAGACGACGGTATCGTTTGGTCGCGCGACTTGCGCGCCAACCGCCGCCGGTaaa < 1:1181758/56‑1 (MQ=255) aaCAGACGACGGTATCGTTTGGTCGCGCGACTTGCGCGCCAACCGCCGCCGGTAAACCaa < 1:573832/59‑1 (MQ=255) cAGACGACGGTATCGTTTGGTCGCGCGACTTGCGCGCCAACCGCCGCCGGTAAACCAAAACCCATGGTGcc > 1:4090211/1‑71 (MQ=38) aGACGACGGTATCGTTTGGTCGCGCGACTTGCGCGCCAAcc < 1:1784896/41‑1 (MQ=37) aGACGACGGTATCGTTTGGTCGCGCGACTTGCGCGCCAACCGCCGCCGGTAAACCa < 1:5202355/56‑1 (MQ=255) acgaCGGTATCGTTTGGTCGCGCGACTTGCGCGCCAACCGCCGCCGGTAAACCAAAACCCATGGTGCCTa < 1:3681018/67‑1 (MQ=38) cgaCGGTATCGTTTGGTCGCGCGACTTGCGCGCCAACCGCCGCCGGTaaa < 1:1102068/48‑1 (MQ=255) cGGTATCGTTTGGTCGCGCGACTTGCGCGCCAACCGCCGCCGGTAAACCAAAACCCATGGTGCCTAAGcc > 1:4305965/1‑70 (MQ=38) gTTTGGTCGCGCGACTTGCGCGCCAACCGCCGCCGGTAAACCAAAACCCATGGTGCCTAAGCCGCTGGAgg > 1:1050522/1‑71 (MQ=38) gTTTGGTCGCGCGACTTGCGCGCCAACCGCCGCCGGTAAACCAAAACCCATGGTGCCTAAGCCGCTGGAg < 1:920427/70‑1 (MQ=38) tCGCGCGACTTGCGCGCCAACCGCCGCCGGTAAACCAAAACCCATGGTGCCTAAGCCGCTGGAGGtgatga < 1:1172482/71‑1 (MQ=38) gcgcgACTTGCGCGCCAACCGCCGCCGGTAAACCAAAACCCATGGTGCCTAAGCCGCTGGAGGTGATGaa < 1:5333707/70‑1 (MQ=38) cgcgACTTGCGCGCCAACCGCCGCCGGTAAACCAAAACCCATGGTGCCTAAGCCg > 1:353476/1‑55 (MQ=37) gcgACTTGCGCGCCAACCGCCGCCGGTAAACCAAAACCCATGGTGCCTAAGCCGCTGGAGGTGATGAAAtt > 1:1490907/1‑71 (MQ=38) cgACTTGCGCGCCAACCGCCGCCGGTAAACCAAAACCCATGGTGCCTAAGCCGCTGGAGGTGATGAAAtt < 1:4372860/70‑1 (MQ=38) gACTTGCGCGCCAACCGCCGCCGGTAAACCAAAACCCATGGTGCCTa > 1:1592633/1‑47 (MQ=25) gACTTGCGCGCCAACCGCCGCCGGTAAACCAAAACCCATGGTGCCTAAGCCGCTGGAGGTGATGAAAt > 1:4094164/1‑68 (MQ=38) aCTTGCGCGCCAACCGCCGCCGGTAAACCAAAACCCATGGTGCCTAAGCCGCTGGAGGTGATGAAATTTTc < 1:5250204/71‑1 (MQ=38) cTTGCGCGCCAACCGCCGCCGGTAAACCAAAACCCATGGt < 1:491101/40‑1 (MQ=255) cTTGCGCGCCAACCGCCGCCGGTAAACCAAAACCCATGGTGCCTAAGCCGCTGGAGGTGATGAAATTTTcc < 1:3759027/71‑1 (MQ=38) | GCACATTCATCATGAAAGAGCCGTCACCGGAGATACAGACAACGGTATCGTTCGGTCGCGCGACTTGTGCGCCAACCGCCGCCGGTAAACCAAAACCCATGGTACCTAAACCGCTGGAGGTGATGAAATTTTCC > AP009048/3684818‑3684951 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |