| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | AP009048 | 3,684,921 | A→G | 100% | pseudogene (138/582 nt) pseudogene (1201/1645 nt) |
ilvG ← ilvG ← |
ECK3760:JW3741:b3768; acetolactate synthase II, large subunit, C‑ter fragment ECK3760:JW3740+JW3741:b4488; large subunit of acetolactate synthase II |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AP009048 | 3,684,921 | 0 | A | G | 100.0% | 170.2 / NA | 50 | pseudogene (138/582 nt) pseudogene (1201/1645 nt) | ilvG ilvG | ECK3760:JW3741:b3768; acetolactate synthase II, large subunit, C‑ter fragment ECK3760:JW3740+JW3741:b4488; large subunit of acetolactate synthase II |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (29/21); total (29/21) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
CAGACAACGGTATCGTTCGGTCGCGCGACTTGTGCGCCAACCGCCGCCGGTAAACCAAAACCCATGGTACCTAAACCGCTGGAGGTGATGAAATTTTCCGGGCGAGTGTGGGCGATGTGCTGCGCAGCCCACATC > AP009048/3684853‑3684987 | cAGACGACGGTATCGTTTGGTCGCGCGACTTGCGCGCCAACCGCCGCCGGTAAACCAAAACCCATGGTGcc > 1:4090211/1‑71 (MQ=38) acgaCGGTATCGTTTGGTCGCGCGACTTGCGCGCCAACCGCCGCCGGTAAACCAAAACCCATGGTGCCTa < 1:3681018/67‑1 (MQ=38) cGGTATCGTTTGGTCGCGCGACTTGCGCGCCAACCGCCGCCGGTAAACCAAAACCCATGGTGCCTAAGcc > 1:4305965/1‑70 (MQ=38) gTTTGGTCGCGCGACTTGCGCGCCAACCGCCGCCGGTAAACCAAAACCCATGGTGCCTAAGCCGCTGGAgg > 1:1050522/1‑71 (MQ=38) gTTTGGTCGCGCGACTTGCGCGCCAACCGCCGCCGGTAAACCAAAACCCATGGTGCCTAAGCCGCTGGAg < 1:920427/70‑1 (MQ=38) tCGCGCGACTTGCGCGCCAACCGCCGCCGGTAAACCAAAACCCATGGTGCCTAAGCCGCTGGAGGtgatga < 1:1172482/71‑1 (MQ=38) gcgcgACTTGCGCGCCAACCGCCGCCGGTAAACCAAAACCCATGGTGCCTAAGCCGCTGGAGGTGATGaa < 1:5333707/70‑1 (MQ=38) cgcgACTTGCGCGCCAACCGCCGCCGGTAAACCAAAACCCATGGTGCCTAAGCCg > 1:353476/1‑55 (MQ=37) gcgACTTGCGCGCCAACCGCCGCCGGTAAACCAAAACCCATGGTGCCTAAGCCGCTGGAGGTGATGAAAtt > 1:1490907/1‑71 (MQ=38) cgACTTGCGCGCCAACCGCCGCCGGTAAACCAAAACCCATGGTGCCTAAGCCGCTGGAGGTGATGAAAtt < 1:4372860/70‑1 (MQ=38) gACTTGCGCGCCAACCGCCGCCGGTAAACCAAAACCCATGGTGCCTa > 1:1592633/1‑47 (MQ=25) gACTTGCGCGCCAACCGCCGCCGGTAAACCAAAACCCATGGTGCCTAAGCCGCTGGAGGTGATGAAAt > 1:4094164/1‑68 (MQ=38) aCTTGCGCGCCAACCGCCGCCGGTAAACCAAAACCCATGGTGCCTAAGCCGCTGGAGGTGATGAAATTTTc < 1:5250204/71‑1 (MQ=38) cTTGCGCGCCAACCGCCGCCGGTAAACCAAAACCCATGGTGCCTAAGCCGCTGGAGGTGATGAAATTTTcc < 1:3759027/71‑1 (MQ=38) gcgcgcCAACCGCCGCCGGTAAACCAAAACCCATGGTGCCTa < 1:3753056/40‑1 (MQ=25) gcgcgcCAACCGCCGCCGGTAAACCAAAACCCATGGTGCCTAAGCCGCTGGAGGTGATGAAATTTTCCggg > 1:5159605/3‑71 (MQ=38) cgcgcCAACCGCCGCCGGTAAACCAAAACCCATGGTGCCTAAGCCGCTGGAGGTGATGAAATTTTCCgg < 1:1714868/68‑1 (MQ=38) cAACCGCCGCCGGTAAACCAAAACCCATGGTGCCTAAGCCGCTGGAGGTGATGAAATTTTCCGGGCGAgtg > 1:3912906/1‑71 (MQ=255) cAACCGCCGCCGGTAAACCAAAACCCATGGTGCCTAAGCCGCTGGAGGTGATGAAATTTTCCGGGCGAgtg > 1:2072508/1‑71 (MQ=255) cAACCGCCGCCGGTAAACCAAAACCCATGGTGCCTAAGCCGCTGGAGGTGATGAAATTTTCCGGGCGAgtg > 1:2224103/1‑71 (MQ=255) aaCCGCCGCCGGTAAACCAAAACCCATGGTGCCTAAGCCGCTGGAGGtgatga < 1:3614075/53‑1 (MQ=37) aCCGCCGCCGGTAAACCAAAACCCATGGTGCCTAAGCCGCTGGTGGTGATGAAATTTTCCGGGCGAGtgtg > 1:566862/1‑71 (MQ=38) aCCGCCGCCGGTAAACCAAAACCCATGGTGCCTAAGCCGCTGGAGGtgatga < 1:4341718/52‑1 (MQ=37) aCCGCCGCCGGTAAACCAAAACCCATGGTGCCTAAGCCGCTGGAGGtgatga < 1:3499101/52‑1 (MQ=37) aCCGCCGCCGGTAAACCAAAACCCATGGTGCCTAAGCCGCTGGAGGTGATGAAATTTTCCGGGCGAGtgtg > 1:5033345/1‑71 (MQ=255) cgccgccgGTAAACCAAAACCCATGGTGCCTAAGCCGCTGGAGGTGATGAAATTTTCCGGGCGAGTGTggg > 1:1993034/1‑71 (MQ=255) gacgGTAAACCAAAACCCATGGTGCCTAAGCCGCTGGAGGTGATGAAATTTTCCGGGCGAGTGTGGGCGAt > 1:227374/3‑71 (MQ=39) ggTAAACCAAAACCCATGGTGCCTAAGCCGCTGGAGGTGATGAAATTTTCCGGGCGAGTGTGGGCGAtgtg > 1:600505/1‑71 (MQ=255) tAAACCAAAACCCATGGTGCCTAAGCCGCTGGAGGTGATGAAAtttt < 1:1346206/47‑1 (MQ=38) tAAACCAAAACCCATGGTGCCTAAGCCGCTGGAGGTGATGAAATTTTc < 1:3093736/48‑1 (MQ=38) tAAACCAAAACCCATGGTGCCTAAGCCGCTGGAGGTGATGAAATTTTCCGGGCGAGTGTGGGCGATGtgct > 1:1331454/1‑71 (MQ=255) aaaCCAAAACCCATGGTGCCTAAGCCGCTGGAGGTGATGAAATTTTCCGGGCGAGTGTGGGCGATGtgctg > 1:1575504/1‑71 (MQ=255) aaCCAAAACCCATGGTGCCTAAGCCGCTGGAGGTGATGAAATTTTcc > 1:1973795/1‑47 (MQ=38) aCCAAAACCCATGGTGCCTAAGCCGCTGGAGGTGATGAAATTTTCCGGGCGAGTGTGGGCGATGTGCTgcg > 1:2523448/1‑71 (MQ=255) ccAAAACCCATGGTGCCTAAGCCGCTGGAGGTGATGAAATTTTcc < 1:3364557/45‑1 (MQ=255) cAAAACCCATGGTGCCTAAGCCGCTGGAGGTGATGAAATTTTGCGGGCGAGTGTGGGCGATGTGCTGCGCa < 1:1338908/71‑1 (MQ=255) cAAAACCCATGGTGCCTAAGCCGCTGGAGGTGATGAAATTTTCCGGGCGa > 1:2439332/1‑50 (MQ=255) cAAAACCCATGGTGCCTAAGCCGCTGGAGGTGATGAAATTTTCCGGGCGAGTGTGGGCGATGTGCTgcg > 1:5343187/1‑69 (MQ=255) cAAAACCCATGGTGCCTAAGCCGCTGGAGGTGATGAAATTTTCCGGGCGAGTGTGGGCGATGTGCTgcg > 1:4874716/1‑69 (MQ=255) aaaaCCCATGGTGCCTAAGCCGCTGGAGGTGATGAAATTTTCCGGGCGAGTGTGGGCGATGTGCTGCGCAg > 1:399682/1‑71 (MQ=255) aaaaCCCATGGTGCCTAAGCCGCTGGAGGTGATGAAATTTTCCGGGCGAGTGTGGGCGATGTGCTGCGCAg > 1:3966328/1‑71 (MQ=255) aaaCCCATGGTGCCTAAGCCGCTGGAGGTGATGAAATTTTcc > 1:4430688/1‑42 (MQ=38) aaaCCCATGGTGCCTAAGCCGCTGGAGGTGATGAAATTTTCCGGGCGAGTGTGGGCGATGCGCTGCGCAg > 1:1000305/1‑70 (MQ=255) aaCCCATGGTGCCTAAGCCGCTGGAGGTGATGAAAtt > 1:2549349/1‑37 (MQ=38) aaCCCATGGTGCCTAAGCCGCTGGAGGTGATGAAATTTTCCGGGCGAgt < 1:2553309/49‑1 (MQ=39) aTGGTGCCTAAGCCGCTGGAGGTGATGAAATTTTCCGGGCg < 1:1754082/41‑1 (MQ=38) aTGGTGCCTAAGCCGCTGGAGGTGATGAAATTTTCCGGGCg < 1:815640/41‑1 (MQ=38) aTGGTGCCTAAGCCGCTGGAGGTGATGAAATTTTCCGGGCGAGTGTggg > 1:4083062/1‑49 (MQ=38) aTGGTGCCTAAGCCGCTGGAGGTGATGAAATTTTCCGGGCGAGTGTGGGCGATGtgct < 1:30153/58‑1 (MQ=39) tGGTGCCTAAGCCGCTGGAGGTGATGAAATTTTCCGGGCGAGTGTGGGCGATGTGCTGCGCAGCCCACATc < 1:4982488/71‑1 (MQ=255) | CAGACAACGGTATCGTTCGGTCGCGCGACTTGTGCGCCAACCGCCGCCGGTAAACCAAAACCCATGGTACCTAAACCGCTGGAGGTGATGAAATTTTCCGGGCGAGTGTGGGCGATGTGCTGCGCAGCCCACATC > AP009048/3684853‑3684987 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |