| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | AP009048 | 3,825,204 | C→T | 100% | D186D (GAC→GAT) | pyrE → | orotate phosphoribosyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AP009048 | 3,825,204 | 0 | C | T | 100.0% | 303.5 / NA | 101 | D186D (GAC→GAT) | pyrE | orotate phosphoribosyltransferase |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (41/60); total (41/60) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
Only 100 of 101 total aligned reads displayed.
ATTTCGGCGATTCAGGAAGTTGAGCGTGATTACAACTGCAAAGTGATCTCTATCATCACCCTGAAAGACCTGATTGCTTACCTGGAAGAGAAGCCGGAAATGGCGGAACATCTGGCGGCGGTTAAGGCCTATCG > AP009048/3825136‑3825269 | aTTTCGGCGATTCAGGAAGTTGAGCGTGATTACAACTGCAAAGTGATCTCTATCATCACCCTGAAAGATCt > 1:3633801/1‑71 (MQ=255)aTTTCGGCGATTCAGGAAGTTGAGCGTGATTACAACTGCAAAGTGATCTCTATCAACACCATGAAAGATCt < 1:2278364/71‑1 (MQ=255) ttCGGCGATTCAGGAAGTTGAGCGTGATTACAACTGCAAAGTGATCTCTATCATCACCCTGAAAGATCTGa > 1:2380957/1‑71 (MQ=255) tCGGCGATTCAGGAAGTTGAGCGTGATTACAACTGCAAAGTGATCTCTATCATCACCCTGAAAGATCTGa < 1:1655173/70‑1 (MQ=255) cGGCGATTCAGGAAGTTGAGCGTGATTACAACTGCAAAGTGATCTCTATCATCACCCTGAAAGATCTGAt < 1:293344/70‑1 (MQ=255) ggCGATTCAGGAAGTTGAGCGTGATTACAACTGCAAAGTGATCTCTATCATCACCCTGAAAGATCTGAtt < 1:2078537/70‑1 (MQ=255) gCGATTCAGGAAGTTGAGCGTGATTACAACTGCAAAGTGATCTCTATCATCACCCTGAAAGATCTGATTGc < 1:2712753/71‑1 (MQ=255) cGATTCAGGAAGTTGAGCGTGATTACAACTGCAAAGTGATCTCTATCATCACCCTGAAAGATCTGATTGcc > 1:185637/1‑70 (MQ=255) cGATTCAGGAAGTTGAGCGTGATTACAACTGCAAAGTGATCTCTATCATCACCCTGAAAGATCTGATTGcc > 1:2928930/1‑70 (MQ=255) gATTCAGGAAGTTGAGCGTGATTACAACTGCAAAGTGATCTCTATCATCACCCTGAAAGATCTGATTGcct < 1:4061545/71‑3 (MQ=255) gATTCAGGAAGTTGAGCGTGATTACAACTGCAAAGTGATCTCTATCATCACCCTGAAAGATCTGATTGcc > 1:1974429/1‑69 (MQ=255) ttCAGGAAGTTGAGCGTGATTACAACTGCAAAGTGATCTCTATCATCACCCTGAAAGATCTGa > 1:5155908/1‑63 (MQ=255) tCAGGAAGTTGAGCGTGATTACAACTGCAAAGTGATCTCTATCATCACCCTGAAAGATCTGATTGCCTatc > 1:1380045/1‑69 (MQ=255) cAGGAAGTTGAGCGTGATTACAACTGCAAAGTGATCTCTATCATCACCCTGAAAGATCTGATTGCCTATCt > 1:723800/1‑71 (MQ=255) aGGAAGTTGAGCGTGATTACAACTGCAAAGTGATCTCTATCATCACCCTGAAAGATCTGATTGCCTATCt > 1:225641/1‑70 (MQ=255) ggAAGTTGAGCGTGATTACAACTGCAAAGTGATCTCTATCATCACCCTGAAAGATCTGATTGCCTATCTgg < 1:5199028/71‑1 (MQ=255) ggAAGTTGAGCGTGATTACAACTGCAAAGTGATCTCTATCATCACACTGAAAGATCTGATTGCCTATCTg > 1:3569649/1‑70 (MQ=255) aaGTTGAGCGTGATTACAACTGCAAAGTGATCTCTATCATCACCCTGAAAGATCTGATTGCCTatc > 1:3972867/1‑64 (MQ=255) aaGTTGAGCGTGATTACAACTGCAAAGTGATCTCTATCATCACCCTGAAAGATCTGATTGCCTATCTgg > 1:4552958/1‑69 (MQ=255) aaGTTGAGCGTGATTACAACTGCAAAGTGATCTCTATCATCACCCTGAAAGATCTGATTGCCTATCTgg > 1:1136426/1‑69 (MQ=255) aaGTTGAGCGTGATTACAACTGCAAAGTGATCTCTATCATCACCCTGAAAGATCTGATTGCCTATCTGGaa < 1:1989433/71‑1 (MQ=255) aaGTTGAGCGTGATTACAACTGCAAAGTGATCTCTATCATCACCCTGAAAGATCTGATTGCCTATCTGCaa < 1:530275/71‑1 (MQ=255) aGTTGAGCGTGATTACAACTGCAAAGTGATCTCTATCATCACCCTGAAAGATCTGATTGCCTATCTgg > 1:2727574/1‑68 (MQ=255) aGTTGAGCGTGATTACAACTGCAAAGTGATCTCTATCATCACCCTGAAAGATCTGATTGCCTATCTgg > 1:3746999/1‑68 (MQ=255) ttGAGCGTGATTACAACTGCAAAGTGATCTCTATCATCACCCTGAAAGATCt < 1:2336354/52‑1 (MQ=255) ttGAGCGTGATTACAACTGCAAAGTGATCTCTATCATCACCCTGAAAGATCTg < 1:5052847/53‑1 (MQ=255) ttGAGCGTGATTACAACTGCAAAGTGATCTCTATCATCACCCTGAAAGATCTGATTGCCTATCTGGAagag < 1:1824974/71‑1 (MQ=255) ttGAGCGTGATTACAACTGCAAAGTGATCTCTATCATCACCCTGAAAGATCTGATTGCCTATCTGGAagag < 1:4766633/71‑1 (MQ=255) ttGAGCGTGATTACAACTGCAAAGTGATCTCTATCATCACCCTGAAAGATCTGATTGCCTATCTGGAagag < 1:656157/71‑1 (MQ=255) ttGAGCGTGATTACAACTGCAAAGTGATCTCTATAATCACCCTGAAAGATCTGATTGCCTATCTGGAagag < 1:207557/71‑1 (MQ=255) aGCGTGATTACAACTGCAAAGTGATCTCTATCATCACCCTGAAAGATCTGATTGCCTATCTGGAagag < 1:4908944/68‑1 (MQ=255) aGCGTGATTACAACTGCAAAGTGATCTCTATCATCACCCTGAAAGATCTGATTGCCTATCTGGAAGAGaa < 1:2769300/70‑1 (MQ=255) aGCGTGATTACAACTGCAAAGTGATCTCTATCATCACCCTGAAAGATCTGATTGCCTATCTGGAAGAGaa < 1:3992676/70‑1 (MQ=255) aGCGTCATTACAACTGCAAAGTGATCTCTATCATCACCCTGAAAGATCTGATTGCCTATCTTGAagag < 1:2199811/68‑1 (MQ=255) cGTGATTACAACTGCAAAGTGATCTCTATCATCACCCTGAAAGATCTGATTGCCTATCTGGAAGAGAAGcc > 1:3836769/1‑71 (MQ=255) cGTGATTACAACTGCAAAGTGATCTCTATCATCACCCTGAAAGATCTGATTGCCTATCTGGAAGAGAAGcc < 1:3579771/71‑1 (MQ=255) cGTGATTACAACTGCAAAGTGATCTCTATCATCACCCTGAAAGATCTGATTGCCTATCTGGAAGAGAAGcc > 1:2159176/1‑71 (MQ=255) cGTGATTACAACTGCAAAGTGATCTCTATCATCACCCTGAAAGATCTGATTGCCTATCTGGAAGAGAAGcc < 1:1583298/71‑1 (MQ=255) tGATTACAACTGCAAAGTGATCTCTATCATCACCCTGAATGATCTGATTGCCTATCTGGAaga < 1:4077854/63‑1 (MQ=255) tGATTACAACTGCAAAGTGATCTCTATCATCACCCTGAAAGATCTGATTGCCTat > 1:3024279/1‑54 (MQ=255) tGATTACAACTGCAAAGTGATCTCTATCATCACCCTGAAAGATCTGATTGCCTATCTgg < 1:2849197/59‑1 (MQ=255) tGATTACAACTGCAAAGTGATCTCTATCATCACCCTGAAAGATCTGATTGCCTATCTGGAaga > 1:1487725/1‑63 (MQ=255) gATTACAACTGCAAAGTGATCTCTATCATCACCCTGAAAGATCTGATTGCCTATCTGGAagag < 1:4516012/63‑1 (MQ=255) aTTACAACTGCAAAGTGATCTCTATCATCACCCTGAAAGATCTGATTGCCTATCTGGAAGAGAAGCCGGa < 1:5199474/70‑1 (MQ=255) aTTACAACTGCAAAGTGATCTCTATCATCACCCTGAAAGATCTGATTGCCTATCTGGAAGAGAAGCCGGa < 1:1628120/70‑1 (MQ=255) aTTACAACTGCAAAGTGATCTCTATCATCACCCTGAAAGATCTGATTGCCTATCTGGAAGAGAAGCCGGa > 1:4544600/1‑70 (MQ=255) ttACAACTGCAAAGTGATCTCTATCATCACCCTGAAAGATCTGATTGCCTATCTGGaa < 1:253477/58‑1 (MQ=38) ttACAACTGCAAAGTGATCTCTATCATCACCCTGAAAGATCTGATTGCCTATCTGGAAGAGAAGCCGGaaa < 1:2217108/71‑1 (MQ=255) ttACAACTGCAAAGTGATCTCTATCATCACCCTGAAAGATCTGATTGCCTATCTGGAAGAGAAGCCGGaaa > 1:3635434/1‑71 (MQ=255) ttACAACTGCAAAGTGATCTCTATCATCACACTGAAAGATCTGATTGCCTATCTGGaa < 1:4911055/58‑1 (MQ=38) aCATCTGCAATGTGATCTCTATCATCACCCTGAAAGATCTGATTGCCTATCTGGAAGAGTAGCCGGAAAt < 1:1480923/70‑1 (MQ=255) aCAACTGCAAAGTGATCTCTATCATCACCCTGAAAGATCTGATTGCCTATCTg < 1:123844/53‑1 (MQ=255) aCAACTGCAAAGTGATCTCTATCATCACCCTGAAAGATCTGATTGCCTATCTGGAAGAGAAGCCGGAAATg > 1:1658584/1‑71 (MQ=255) cAACTGCAAAGTGATCTCTATCATCACCCTGAAAGATCt < 1:1603529/39‑1 (MQ=255) cAACTGCAAAGTGATCTCTATCATCACCCTGAAAGATCTg < 1:2757576/40‑1 (MQ=255) cAACTGCAAAGTGATCTCTATCATCACCCTGAAAGATCTGATTg > 1:382461/1‑44 (MQ=255) cAACTGCAAAGTGATCTCTATCATCACCCTGAAAGATCTGATTg > 1:1351775/1‑44 (MQ=255) cAACTGCAAAGTGATCTCTATCATCACCCTGAAAGATCTGATTGCCTATCTGGAagag < 1:1583524/58‑1 (MQ=38) cAACTGCAAAGTGATCTCTATCATCACCCTGAAAGATCTGATTGCCTATCTGGAAGAGAAGCCGGAAATg < 1:4913651/70‑1 (MQ=255) cAACTGCAAAGT‑ATCTCTATCATCACCCTGAAAGATCTGATTGCCTATCTGGAAGAGAAGCCGGAAATgg < 1:4058534/70‑1 (MQ=255) aaCTGCAAAGTGATCTCTATCATCACCCTGAAAGATCTGATTGCCTATCTGGAAGATAAGCCGGAAATGGc < 1:2026270/71‑1 (MQ=255) cTGCAAAGTGATCTCTATCATCACCCTGAAAGATCTGATTGCCTATCTGGAAGAGAAGCCGGAAATGGCgg < 1:2966120/71‑1 (MQ=255) cTGCAAAGTGATCTCTATCATCACCCTGAAAGATCTGATTGCCTATCTGGAAGAGAAGCCGGAAATGGCgg < 1:233654/71‑1 (MQ=255) cTGCAAAGTGATCTCTATCATCACCCTGAAAGATCTGATTGCCTATCTGGAAGAGAAGCCGGAAATGGCg > 1:4700676/1‑70 (MQ=255) gCAAAGTGATCTCTATCATCACCCTGAAAGATCTGATTGCCTATCTGGAAGAGAAGCCGGAAATGGc < 1:573974/67‑1 (MQ=255) cAAAGTGATCTCTATCATCACCCTGAAAGATCTGATTGCCTATCTGGAAGAGAAGCCGGAAATGGc < 1:749294/66‑1 (MQ=255) cAAAGTGATCTCTATCATCACCCTGAAAGATCTGATTGCCTATCTGGAAGAGAAGCCGGAAATGGCGGa < 1:1551747/69‑1 (MQ=255) aaaGTGATCTCTATCATCACCCTGAAAGATCTGATTGCCTATCTGGAAGAGAAGCCGGAAATg < 1:2053783/63‑1 (MQ=255) aaaGTGATCTCTATCATCACCCTGAAAGATCTGATTGCCTATCTGGAAGAGAAGCCGGAAATGGCGGAACa < 1:3771984/71‑1 (MQ=255) aaGTGATCTCTATCATCACCCTGAAAGATCTGATTGCCTATCTGGAaga > 1:1761266/1‑49 (MQ=37) aaGTGATCTCTATCATCACCCTGAAAGATCTGATTGCCTATCTGGAAGAGAAGCCGGAAATGGCGGAACAt < 1:2255514/71‑1 (MQ=255) tGATCTCTATCATCACCCTGAAAGATCTGATTGCCTATCTGGAAGAGAAGCCGGaaa > 1:145823/1‑57 (MQ=38) tGATCTCTATCATCACCCTGAAAGATCTGATTGCCTATCTGGAAGAGAAGCCGGAAATGGCGGAACATCTg < 1:3027874/71‑1 (MQ=255) aTCTCTATCATCACCCTGAAAGATCTGATTGCCTATCTGGAAGAGAAGCCGGaaa > 1:517287/1‑55 (MQ=38) aTCTCTATCATCACCCTGAAAGATCTGATTGCCTATCTGGAAGAGAAGCCGGaa > 1:523616/1‑54 (MQ=38) tctATCATCACCCTGAAAGATCTGATTGCCTATCTGGAAGAGAAg > 1:4099755/1‑45 (MQ=37) tctATCATCACCCTGAAAGATCTGATTGCCTATCTGGAAGAGAAGCCGGAAAt > 1:2108268/1‑53 (MQ=38) ctATCATCACCCTGAAAGATCTGATTGCCTATCTGGAAGAGAAGCCGGAAATGGCGGAACATCTGgcggcg > 1:64746/1‑71 (MQ=255) ctATCATCACCCTGAAAGATCTGATTGCCTATCTGGAAGAGAAGCCGGAAATGGCGGAACATCTGgcggcg > 1:70968/1‑71 (MQ=255) tATCATCACCCTGAAAGATCTGATTGCCTATCTGGAAGAGAAGCCGGAAATGGCGGAACATCTggcgg < 1:2623498/68‑1 (MQ=255) tATCATCACCCTGAAAGATCTGATTGCCTATCTGGAAGAGAAGCCGGAAATGGCGGAACATCTGGcggcgg > 1:2702396/1‑71 (MQ=255) atcatcACCCTGAAAGATCTGATTGCCTATCTGGAAGAGAAGCCGGAAATGGCg < 1:2836143/54‑1 (MQ=38) tcatcaCCCTGAAAGATCTGATTGCCTATCTGGAAGAGAAGCCGGAAATGGCGGAACATCTGGCGGCGGtt < 1:1115469/71‑1 (MQ=255) tcatcaCCCTGAAAGATCTGATTGCCTATCTGGAAGAGAAGCCGGAAATGGCGGAACATCTGGCGGCGGtt < 1:3833124/71‑1 (MQ=255) tcatcaCCCTGAAAGATCTGATTGCCTATCTGGAAGAGAAGCCGGAAATGGCGGAACATCTGGCGGCGGtt < 1:2710251/71‑1 (MQ=255) tcatcaCCCTGAAAGATCTGATTGCCTATCTGGAAGAGAAGCCGGAAATGGCGGAACATCTGGCGGCGGtt < 1:1797598/71‑1 (MQ=255) atcaCCCTGAAAGATCTGATTGCCTATCTGGAAGAGAAGCCGGAAATGGCGGAACATCTGGCGGCGGtt > 1:4324868/1‑69 (MQ=39) atcaCCCTGAAAGATCTGATTGCCTATCTGGAAGAGAAGCCGGAAATGGCGGAACATCTGGCGGCGGTTaa < 1:1045871/71‑1 (MQ=255) atcaCCCTGAAAGATCTGATTGCCTATCTGGAAGAGAAGCCGGAAATGGCGGAACATCTGGCGGCGGTTaa < 1:1685945/71‑1 (MQ=255) atcaCCCTGAAAGATCTGATTGCCTATCTGGAAGAGAAGCCGGAAATGGCGGAACATCTGGCGGCGGTTa < 1:4748167/70‑1 (MQ=255) atcaCCCTGAAAGATCTGATTGCCTATCTGGAAGAGAAGCCGGAAATGGCGGAACATCTGGCGGCGGTTa < 1:3237067/70‑1 (MQ=255) tcaCCCTGAAAGATCTGATTGCCTATCTGGAAGAGAAGCCGGAAATGGCGGAACATCTGGCGGCGGTTaa < 1:3568076/70‑1 (MQ=255) aCCCTGAAAGATCTGATTGCCTATCTGGAAGAGAAGCCGGAAATGGCGGAACATCTGGCGGCGGTTAAGGc > 1:2270666/1‑71 (MQ=255) ccTGAAAGATCTGATTGCCTATCTGGAAGAGAAGCCGGAAATGGCgg < 1:2969039/47‑1 (MQ=37) ccTGAAAGATCTGATTGCCTATCTGGAAGAGAAGCCGGAAATGGCGGAACATCTGGCGGCGGTTAAGGcc > 1:1203659/1‑70 (MQ=255) cTGAAAGATCTGATTGCCTATCTGGAAGAGAAGCCGGAAATGGCGGAACATCTGGCGGCGGTTAAGGCCTa < 1:437362/71‑1 (MQ=255) tGAAAGATCTGATTGCCTATCTGGAAGAGAAGCCGGAAATGGCGGAACATCTGGCGGCGGTTAAGGCCTAt > 1:1469702/1‑71 (MQ=255) aaaGATCTGATTGCCTATCTGGAAGAGAAGCCGGAAAt > 1:1096724/1‑38 (MQ=21) aaaGATCTGATTGCCTATCTGGAAGAGAAGCCGGAAATGGCGGAACATCTGGCGGCGGTTAAGGCCTATcg > 1:1116052/1‑71 (MQ=255) aGATCTGATTGCCTATCTGGAAGAGAAGCCGGAAATGGCgg < 1:4251447/41‑1 (MQ=25) | ATTTCGGCGATTCAGGAAGTTGAGCGTGATTACAACTGCAAAGTGATCTCTATCATCACCCTGAAAGACCTGATTGCTTACCTGGAAGAGAAGCCGGAAATGGCGGAACATCTGGCGGCGGTTAAGGCCTATCG > AP009048/3825136‑3825269 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |