| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | AP009048 | 71,214 | T→C | 29.9% | F276F (TTT→TTC) | araC → | DNA‑binding transcriptional dual regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AP009048 | 71,214 | 0 | T | C | 29.9% | 107.4 / 63.8 | 77 | F276F (TTT→TTC) | araC | DNA‑binding transcriptional dual regulator |
| Reads supporting (aligned to +/- strand): ref base T (27/27); new base C (12/11); total (39/38) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.81e-01 | |||||||||||
GTTTTGACGATCAACTCTATTTCTCGCGAGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTTCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAATTGGTAACGAATCAGA > AP009048/71147‑71281 | gTTTTGACGATCAACTCTATTTCTCGCGAGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTTCg < 1:4748361/70‑1 (MQ=255) ttGACGATCAACTCTATTTCTCGCGAGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTTCGTGc < 1:5128710/70‑1 (MQ=255) ttGAAGATCAACTCTATTTCTCGCGAGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTTCGTGc < 1:2964427/70‑1 (MQ=255) tCAACTCTATTTCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTtgt > 1:317514/1‑70 (MQ=255) tttCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGaaa < 1:4615251/68‑1 (MQ=255) tttCTCGCGAGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTTCGTGCCGGTTGTGAAGaaa < 1:1400938/68‑1 (MQ=255) tctcGCGAGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTTCGTGCCGGTTGTGAAGAAAAAGt > 1:549115/1‑70 (MQ=255) tctcGCGAGTATTTAAAAAATGCACAGGGGCCAGCCCGAGCGAGTTTCGTGCCGGTTGTGAAGAAAAAGt < 1:2729000/70‑1 (MQ=255) ctcGCGGGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTg < 1:583383/70‑1 (MQ=255) ctcGCGAGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTTCGTGCCGGTTGTGAAGAAAAAGTg < 1:2639173/70‑1 (MQ=255) cgAGAATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTTCGTGCCGGTtgtg > 1:5367163/1‑55 (MQ=255) gAGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTTCGTGCCGGTTGTGAAGAAAAAGTGAatgat > 1:1397413/1‑71 (MQ=255) aGTATTTAAAAAATGCACCGGGGCCAGCACGAGCGAGTTTCGTGCCGGTTGTGAAGAAAAAGTGAatgatg < 1:1097584/71‑1 (MQ=255) tATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTgaag < 1:591246/54‑1 (MQ=255) aTTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGaaa < 1:5370207/56‑1 (MQ=255) tttAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTTCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGc > 1:2906428/1‑71 (MQ=255) tAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTTCGTGCCGGTtgt > 1:2328841/1‑46 (MQ=255) tAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTTCGTGCCGGTTGTGAAGaaa < 1:4017268/53‑1 (MQ=255) tAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTTCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGcc > 1:3477600/1‑70 (MQ=255) aaaaaaTGCACCGGGGCCAGCCCGAGCGAGTTTCGTGc > 1:1987420/1‑38 (MQ=255) aaaaaaTGCACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGt > 1:656817/1‑71 (MQ=255) aaaaaTGCACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCg < 1:1818348/69‑1 (MQ=255) aaaaTGCACCGGGGCCAGCCCGAGCGAGTTTCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTc < 1:1471173/70‑1 (MQ=255) aaaaTGCACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTc > 1:5274842/1‑70 (MQ=255) aaaaTGCACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCa < 1:344445/71‑1 (MQ=255) aaaTGCACCGGGGCCAGCCCGAGCGAGTTTCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTc > 1:1431764/1‑69 (MQ=255) tGCACCGGGGCCAGCCCGAGCGAGTTTCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGtt > 1:3787250/1‑71 (MQ=255) cACCGGGGCCAGCCCGAGCGAGTTTCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTg < 1:1362352/70‑1 (MQ=255) cACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGt > 1:3783011/1‑71 (MQ=255) aCCGGGGCCAGCCCGAGCGAGTTTCGTGCCGGTTGTGAAGaaa < 1:2270166/43‑1 (MQ=255) aCCGGGGCCAGCCCGAGCGAGTTTCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGt > 1:170011/1‑70 (MQ=255) aCCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGt > 1:4322474/1‑70 (MQ=255) cGGGGCCAGCCCGAGCGAGTTTCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCa > 1:2322955/1‑70 (MQ=255) cGGGGCCAGCCCGAGCGAGTTTCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCa > 1:5293410/1‑70 (MQ=255) ggggCCAGCCCGAGCGAGTTTCGTGCCGGTTGTgaaga < 1:2728928/38‑1 (MQ=255) ggggCCAGCCCGAGCGAGTTTCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCAt < 1:4154375/70‑1 (MQ=255) ggggCCAGCCCGAGCGAGTTTCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATa < 1:2749120/71‑1 (MQ=255) ggggCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATa < 1:2727368/71‑1 (MQ=255) ggCCAGCCCGAGCGAGTTTCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAAt > 1:4214309/1‑71 (MQ=255) gCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAAag > 1:2480447‑M1/1‑69 (MQ=255) ccAGCCCGAGCGAGTTTCGTGCCGGTTGTGAAGaaaa < 1:2634455/37‑1 (MQ=255) ccAGCCCGAGCGAGTTTCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGt < 1:1004798/63‑1 (MQ=255) ccAGCCCGAGCGAGTTTCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATa < 1:362209/67‑1 (MQ=255) ccAGCCCGAGCGAGTTTCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAAt < 1:1222584/69‑1 (MQ=255) ccAGCCCGAGCGAGTTTCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAAt < 1:2448243/69‑1 (MQ=255) cAGCCCGAGCGAGTTTCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTc < 1:513820/55‑1 (MQ=255) cAGCCCGAGCGAGTTCCGTGCCGGTTGTTAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAAag > 1:4868877‑M1/1‑67 (MQ=255) cAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGt < 1:909659/59‑1 (MQ=255) aGCCCGAGCGAGTTTCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAATTGGt < 1:802790/71‑1 (MQ=255) gCCCGAGCGAGTTTCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTc < 1:1383579/53‑1 (MQ=255) gCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAg > 1:960183/1‑56 (MQ=255) cccGAGCGAGTTTCGTGCCGGTTGTGAAGAAAAAGTGATTGATGTAGCCGTCAAGt > 1:2726838/1‑56 (MQ=255) cccGAGCGAGTTTCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAAt > 1:4097156/1‑65 (MQ=255) cccGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAAagggtcc > 1:4928918‑M1/1‑64 (MQ=255) ccGAGCGAGTTTCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAATTGGTAAc > 1:4034351/1‑71 (MQ=255) ccGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGCCATAAagggtccc > 1:1384858‑M1/1‑63 (MQ=255) cgagcgagTTTCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAATTgg > 1:4169202/1‑66 (MQ=255) cgagcgagTTTCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAATTGGTAAc > 1:4202623/1‑70 (MQ=255) cgagcgagTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATaa > 1:839082/1‑62 (MQ=255) gagcgagTTTCGTGCCGGTTGTGAAGAATAAGTGAATGATGTAGCCGGCAAGtt < 1:2198812/54‑1 (MQ=39) gagcgagTTTCGTGCCGGTTGTGAAGAAAAAGTGaa < 1:3357684/36‑1 (MQ=255) gagcgagTTTCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCaa < 1:4769908/51‑1 (MQ=255) gagcgagTTTCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAATTGGTAACg > 1:1626679/1‑70 (MQ=255) gagcgagTTTCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAATTGGTAACg > 1:1632853/1‑70 (MQ=255) gagcgagTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAAagggtcccta < 1:2290621‑M1/71‑11 (MQ=255) agcgagTTTCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAATTGGTAACGaa > 1:189769/1‑71 (MQ=255) gcgagTTTCGTGCCGGTTGTGAAGAAAAAGTGAatgatg > 1:4979617/1‑39 (MQ=255) gcgagTTTCGTGCCGGTTGTGAAGAAAAAGTGAatgatg > 1:4397880/1‑39 (MQ=255) gcgagTTTCGTGCCGGTTGTGAAGAAAAAGTGAatgatg > 1:4382511/1‑39 (MQ=255) gcgagTTTCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAATTGGTa < 1:3403308/65‑1 (MQ=255) gcgagTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAAagggtccctaag > 1:143539‑M1/1‑59 (MQ=255) cgagTTTCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTc > 1:1389662/1‑46 (MQ=255) cgagTTTCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATaa < 1:4106545/58‑1 (MQ=255) agTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAAagggtccctaagtct < 1:3717012‑M1/71‑16 (MQ=255) gTTTCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAATTGGTAACGAATCAGa > 1:2596396/1‑71 (MQ=255) gTTTCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAATTGGTAACGAATCAGa > 1:5330138/1‑71 (MQ=255) gTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGtt < 1:5084649/48‑1 (MQ=255) tttCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAATTGGTAAc < 1:3625724/62‑1 (MQ=255) tttCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAATTGGTAACGa < 1:2997585/64‑1 (MQ=255) tttCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAATTGGTAACGAATCAg < 1:3517817/69‑1 (MQ=255) tttCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAATTGGTAACGAATCAGa > 1:3330476/1‑70 (MQ=255) tCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCa > 1:2520889/1‑49 (MQ=255) | GTTTTGACGATCAACTCTATTTCTCGCGAGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTTCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAATTGGTAACGAATCAGA > AP009048/71147‑71281 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |