| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 109,553 | T→C | 7.4% | Y599Y (TAT→TAC) | aceE → | pyruvate dehydrogenase, decarboxylase component E1, thiamin‑binding |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 109,553 | 0 | T | C | 7.4% | 244.8 / 8.6 | 81 | Y599Y (TAT→TAC) | aceE | pyruvate dehydrogenase, decarboxylase component E1, thiamin‑binding |
| Reads supporting (aligned to +/- strand): ref base T (32/43); new base C (2/4); total (34/47) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01 | |||||||||||
GTTGTTCCTGGCTGGCAGCGGCGACCTCTTACAGCACCAACAATCTGCCGATGATCCCGTTCTACATCTATTACTCGATGTTCGGCTTCCAGCGTATTGGCGATCTGTGCTGGGCGGCTGGCGACCAGCAAGCGCGTG > Exported/109483‑109620 | gttgttCCTGGCTGGCAGCGGCGACCTCTTACAGCACCAACAATCTGCCGATGATCCCGTTCTACATCTAt > 1:1146880/1‑71 (MQ=255)gttgttCCTGGCTGGCAGCGGCGACCTCTTACAGCACCAACAATCTGCCGATGATCCCGTTCTACATCTAt > 1:3089832/1‑71 (MQ=255)gttgttCCTGGCTGGCAGCGGCGACCTCTTACAGCACCAACAATCTGCCGATGATCCCGTTCTACATCTAt > 1:1307648/1‑71 (MQ=255) tgttCCTGGCTGGCAGCGGCGACCTCTTACAGCACCAACAATCTGCCGATGATCCCGTTCTACATCTAt > 1:1920114/1‑69 (MQ=255) tgttCCTGGCTGGCAGCGGCGACCTCTTACAGCACCAACAATCTGCCGATGATCCCGTTCTACATCTAt > 1:1916129/1‑69 (MQ=255) tgttCCTGGCTGGCAGCGGCGACCTCTTACAGCACCAACAATCTGCCGATGATCCCGTTCTACATCTAt > 1:1319309/1‑69 (MQ=255) tgttCCTGGCTGGCAGCGGCGACCTCTTACAGCACCAACAATCTGCCGATGATCCCGTTCTACATCTATTa > 1:2529305/1‑71 (MQ=255) ccTGGCTGGCAGCGGCGACCTCTTACAGCACCAACAATCTGCCGATGATCCCGTTCTACATCTATTACTc < 1:19056/70‑1 (MQ=255) ccTGGCTGGCAGCGGCGACCTCTTACAGCACCAACAATCTGCCGATGATCCCGTTCTACATCTATTACTc > 1:2245655/1‑70 (MQ=255) gctggcAGCGGCGACCTCTTACAGCACCAACAATCTGCCGATGATCCCGTTCTACATCTATTACTCGATg < 1:2044989/70‑1 (MQ=255) gctggcAGCGGCGACCTCTTACAGCACCAACAATCTGCCGATGATCCCGTTCTACATCTATTACTCGATGt > 1:2557095/1‑71 (MQ=255) gctggcAGCGGCGACCTCTTACAGCACCAACAATCTGCCGATGATCCCGTTCTACATCTATTACTCGATGt > 1:1466066/1‑71 (MQ=255) gcAGCGGCGACCTCTTACAGCACCAACAATCTGCCGATGATCCCGTTCTACATCTATTACTCGATGTTCgg > 1:278808/1‑71 (MQ=255) cggcgACCTCTTACAGCACCAACAATCTGCCGATGATCCCGTTCTACATCTATTACTCGATGTTCGGCTTc < 1:2121426/71‑1 (MQ=255) ggcgACCTCTTACAGCACCAACAATCTGCCGATGATCCCGTTCTACATCTATTACTCGATGTTCGGCTTc > 1:2000590/1‑70 (MQ=255) ccTCTTACAGCACCAACAATCTGCCGATGATCCCGTTCTACATCTATTACTCGATGTTCGGCTTCCAGCGt < 1:1396264/71‑1 (MQ=255) tctTACAGCACCAACAATCTGCCGATGATCCCGTTCTACATCTATTACTCGATGTTCGGCTTCCAGCGt < 1:2319861/69‑1 (MQ=255) tctTACAGCACCAACAATCTGCCGATGATCCCGTTCTACATCTATTACTCGATGTTCGGCTTCCAGCGt < 1:2256146/69‑1 (MQ=255) tctTACAGCACCAACAATCTGCCGATGATCCCGTTCTACATCTATTACTCGATGTTCGGCTTCCAGCGt < 1:1890615/69‑1 (MQ=255) aCAGCACCAACAATCTGCCGATGATCCCGTTCTACATCTATTa > 1:638580/1‑43 (MQ=255) aCAGCACCAACAATCTGCCGATGATCCCGTTCTACATCTATTa > 1:2175198/1‑43 (MQ=255) aCAGCACCAACAATCTGCCGATGATCCCGTTCTACATCTATTACTCGATGTTCGGCTTCCAGCGTAtt < 1:3048325/68‑1 (MQ=255) cAGCACCAACAATCTGCCGATGATCCCGTTCTACATCTATTACTCGATGTTCGGCTTCCAGCGTATTGGCg < 1:252514/71‑1 (MQ=255) cAGCACCAACAATCTGCCGATGATCCCGTTCTACATCTATTACTCGATGTTCGGCTTCCAGCGTATTGGCg < 1:3018301/71‑1 (MQ=255) cAGCACCAACAATCTGCCGATGATCCCGTTCTACATCTATTACTCGATGTTCGGCTTCCAGCGTATTGGCg < 1:2587639/71‑1 (MQ=255) ccAACAATCTGCCGATGATCCCGTTCTACATCTATTACTCGATGTTCGGCTTCCAGCg < 1:647198/58‑1 (MQ=255) ccAACAATCTGCCGATGATCCCGTTCTACATCTATTACTCGATGTTCGGCTTCCAGCg < 1:2822556/58‑1 (MQ=255) ccAACAATCTGCCGATGATCCCGTTCTACATCTATTACTCGATGTTCGGCTTCCAGCg < 1:3291481/58‑1 (MQ=255) ccAACAATCTGCCGATGATCCCGTTCTACATCTATTACTCGATGTTCGGCTTCCAGCg < 1:2818610/58‑1 (MQ=255) aacaaTCTGCCGATGATCCCGTTCTACATCTATTACTCGATGTTCGGCTTCCAGCGTATTGGCGATCtgtg < 1:1208559/71‑1 (MQ=255) aacaaTCTGCCGATGATCCCGTTCTACATCTATTACTCGATGTTCGGCTTCCAGCGTATTGGCGATCtgtg < 1:2206296/71‑1 (MQ=255) aaTCTGCCGATGATCCCGTTCTACATCTATTACTCGATGTTCGGCTTCCAGCGTATTGGCGATCTGTGCTg > 1:3328958/1‑71 (MQ=255) aaTCTGCCGATGATCCCGTTCTACATCTATTACTCGATGTTCGGCTTCCAGCGTATTGGCGATCTGTGCTg > 1:392295/1‑71 (MQ=255) aaTCTGCCGATGATCCCGTTCTACATCTATTACTCGATGTTCGGCTTCCAGCGTATTGGCGATCTGTGCTg > 1:3333516/1‑71 (MQ=255) tGCCGATGATCCCGTTCTACATCTATTACTCGATGTTCGGCTTCCAGCGt < 1:3287767/50‑1 (MQ=255) tGCCGATGATCCCGTTCTACATCTATTACTCGATGTTCGGCTTCCAGCGt < 1:275654/50‑1 (MQ=255) tGCCGATGATCCCGTTCTACATCTATTACTCGATGTTCGGCTTCCAGCGt < 1:310083/50‑1 (MQ=255) tGCCGATGATCCCGTTCTACATCTATTACTCGATGTTCGGCTTCCAGCGTATTGGCGATCTGTGCTGggcg > 1:1117879/1‑71 (MQ=255) tGCCGATGATCCCGTTCTACATCTATTACTCGATGTTCGGCTTCCAGCGTATTGGCGATCTGTGCTGggc > 1:1821543/1‑70 (MQ=255) gCCGATGATCCCGTTCTACATCTATTACTCGATGTTCGGCTTCCAGCGTATTGGCGATCTGTGCTGggcgg > 1:2654079/1‑71 (MQ=255) ccGATGATCCCGTTCTACATCTATTACTCGATGTTCGGCTTCCAGCGTAt > 1:1124003/1‑50 (MQ=255) ccGATGATCCCGTTCTACATCTATTACTCGATGTTCGGCTTCCAGCGTATTGGCGATCTGTGCTGggcgg < 1:2524326/70‑1 (MQ=255) ccGATGATCCCGTTCTACATCTATTACTCGATGTTCGGCTTCCAGCGTATTGGCGATCTGTGCTGggcgg < 1:1675948/70‑1 (MQ=255) ccGATGATCCCGTTCTACATCTATTACTCGATGTTCGGCTTCCAGCGTATTGGCGATCTGTGCTGggc < 1:240507/68‑1 (MQ=255) ccGATGATCCCGTTCTACATCTATTACTCGATGTTCGGCTTCCAGCGTATTGGCGATCTGTGCTGggc < 1:2126904/68‑1 (MQ=255) cGATGATCCCGTTCTACATCTATTACTCGATGTTCGGCTTCCAGCGTATTGGCGATCTGTGCTGggcggc < 1:3141849/70‑1 (MQ=255) cGATGATCCCGTTCTACATCTATTACTCGATGTTCGGCTTCCAGCGTATTGGCGATCTGTGCTGGGCGTCt < 1:3068943/71‑1 (MQ=255) cGATGATCCCGTTCTACATCTATTACTCGATGTTCGGCTTCCAGCGTATTGGCGATCTGTGCTGGGCGGCt < 1:147393/71‑1 (MQ=255) cGATGATCCCGTTCTACATCTATTACTCGATGTTCGGCTTCCAGCGTATTGGCGATCTGTGCTGGGCGGCt < 1:2536238/71‑1 (MQ=255) cGATGATCCCGTTCTACATCTATTACTCGATGTTCGGCTTCCAGCGTATTGGCGATCTGTGCTGGGCGGCt < 1:3129756/71‑1 (MQ=255) cGATGATCCCGTTCTACATCTATTACTCGATGTTCGGCTTCCAGCGTATTGGCGATCTGTGCTGGGCGGCt < 1:39992/71‑1 (MQ=255) cGATGATCCCGTTCTACATCTATTACTCGATGTTCGGCTTCCAGCGTATTGGCGATCTGTGCTGGGCGGCt < 1:1619389/71‑1 (MQ=255) gatgatCCCGTTCTACATCTATTACTCGATGTTCGGCTTCCAGCGTATTGGCGATCTGTGCTGGGCGGCTg < 1:40238/71‑1 (MQ=255) gatgatCCCGTTCTACATCTATTACTCGATGTTCGGCTTCCAGCGTATTGGCGATCTGTGCTGGGCGGCTg < 1:1239705/71‑1 (MQ=255) gatgatCCCGTTCTACATCTATTACTCGATGTTCGGCTTCCAGCGTATTGGCGATCTGTGCTGGGCGGCTg < 1:2449/71‑1 (MQ=255) gatgatCCCGTTCTACACCTTCTATTCGATGTTCGGCATGCAGCgcg > 1:3439500/1‑45 (MQ=255) gatgatCCCGTTCTACACCTTCTATTCGATGTTCGGCATGCAGCgcg > 1:1113445/1‑45 (MQ=255) tgatCCCGTTCTACATCTATTACTCGATGTTCGGCTTCCAGCGTATTGGCGATCTGTGCTGGGCGGCTg < 1:1063523/69‑1 (MQ=255) gatCCCGTTCTACATCTATTACTCGATGTTCGGCTTCCAGCGTATTGGCGATCTGTGCTGGGCGGCTGGc > 1:2559097/1‑70 (MQ=255) tCCCGTTCTACATCTATTACTCGATGTTCGGCTTCCAGCGTATTGGCGATCTGTGc > 1:2858886/1‑56 (MQ=255) tCCCGTTCTACATCTATTACTCGATGTTCGGCTTCCAGCGTATTGGCGATCTGTGc > 1:565164/1‑56 (MQ=255) tCCCGTTCTACATCTATTACTCGATGTTCGGCTTCCAGCGTATTGGCGATCTGTGc > 1:3468185/1‑56 (MQ=255) tCCCGTTCTACATCTATTACTCGATGTTCGGCTTCCAGCGTATTGGCGATCTGTGc > 1:3464395/1‑56 (MQ=255) tCCCGTTCTACATCTATTACTCGATGTTCGGCTTCCAGCGTATTGGCGATCTGTGc > 1:3350921/1‑56 (MQ=255) tCCCGTTCTACATCTATTACTCGATGTTCGGCTTCCAGCGTATTGGCGATCTGTGc > 1:2197836/1‑56 (MQ=255) tCCCGTTCTACATCTATTACTCGATGTTCGGCTTCCAGCGTATTGGCGATCTGTGc > 1:3032563/1‑56 (MQ=255) tCCCGTTCTACATCTATTACTCGATGTTCGGCTTCCAGCGTATTGGCGATCTGTGCTGGGCGGCt < 1:2039057/65‑1 (MQ=255) ccGTTCTACATCTATTACTCGATGTTCGGCTTCCAGCGTATTGGCGATCTGTGCTGGGCGGCTGGCGACCa < 1:2499285/71‑1 (MQ=255) gTTCTACATCTATTACTCGATGTTCGGCTTCCAGCg < 1:416788/36‑1 (MQ=255) gTTCTACATCTATTACTCGATGTTCGGCTTCCAGCg < 1:1535972/36‑1 (MQ=255) gTTCTACATCTATTACTCGATGTTCGGCTTCCAGCGTATTGGCGATCTGTGCTGGGCGGCTGGCGACCAGc < 1:984123/71‑1 (MQ=255) gTTCTACATCTATTACTCGATGTTCGGCTTCCAGCGTATTGGCGATCTGTGCTGGGCGGCTGGCGACCAGc < 1:457173/71‑1 (MQ=255) gTTCTACATCTATTACTCGATGTTCGGCTTCCAGCGTATTGGCGATCTGTGCTGGGCGGCTGGCGACCAGc < 1:1620380/71‑1 (MQ=255) gTTCTACATCTATTACTCGATGTTCGGCTTCCAGCGTATTGGCGATCTGTGCTGGGCGGCTGGCGACCAGc < 1:3087581/71‑1 (MQ=255) ttCTACATCTATTACTCGATGTTCGGCTTCCAGCGTATTGGCGATCTGTGCTGGGCGGCTGGCGACCAGc > 1:934918/1‑70 (MQ=255) cTACATCTATTACTCGATGTTCGGCTTCCAGCGTATTGGCGATCTGTGCTGGGCGGCTGGCGACCAGCAAg > 1:3383298/1‑71 (MQ=255) cTACATCTATTACTCGATGTTCGGCTTCCAGCGTATTGGCGATCTGTGCTGGGCGGCTGGCGACCAGCAAg > 1:179532/1‑71 (MQ=255) cTACATCTATTACTCGATGTTCGGCTTCCAGCGTATTGGCGATCTGTGCTGGGCGGCTGGCGACCAGCAAg > 1:552215/1‑71 (MQ=255) cTACATCTATTACTCGATGTTCGGCTTCCAGCGTATTGGCGATCTGTGCTGGGCGGCTGGCGACCAGCAAg > 1:2171568/1‑71 (MQ=255) cTACATCTATTACTCGATGTTCGGCTTCCAGCGTATTGGCGATCTGTGCTGGGCGGCTGGCGACCAGCAAg > 1:852395/1‑71 (MQ=255) cTACATCTATTACTCGATGTTCGGCTTCCAGCGTATTGGCGATCTGTGCTGGGCGGCTGGCGACCAGCAAg > 1:2501439/1‑71 (MQ=255) ttCTACTACTCGATGTTCGGCTTCCAGCGGACCGGCGACCAGTTCTGGGCGGCTGGc < 1:1753340/56‑1 (MQ=255) ttCTACTACTCGATGTTCGGCTTCCAGCGGACCGGCGACCAGTTCTGGGCGGCTGGc < 1:3110759/56‑1 (MQ=255) ttCTACTACTCGATGTTCGGCTTCCAGCGGACCGGCGACCAGTTCTGGGCGGCTGGc < 1:625303/56‑1 (MQ=255) ttCTACTACTCGATGTTCGGCTTCCAGCGGACCGGCGACCAGTTCTGGGCGGCTGGc < 1:855149/56‑1 (MQ=255) ctactaCTCGATGTTCGGCTTCCAGCGGACCGGCGACCAGTTCTGGGCGGCTGGCGa > 1:1567622/1‑57 (MQ=255) tATTACTCGATGTTCGGCTTCCAGCGTATTGGCGATCTGTGCTGGGCGGCTGGCGACCAGCAAGCGCGTg > 1:2573660/1‑70 (MQ=255) tATTACTCGATGTTCGGCTTCCAGCGTATTGGCGATCTGTGCTGGGCGGCTGGCGACCAGCAAGCGCGTg > 1:1328242/1‑70 (MQ=255) ttACTCGATGTTCGGCTTCCAGCGTATTGGCGATCtg < 1:2786620/37‑1 (MQ=255) | GTTGTTCCTGGCTGGCAGCGGCGACCTCTTACAGCACCAACAATCTGCCGATGATCCCGTTCTACATCTATTACTCGATGTTCGGCTTCCAGCGTATTGGCGATCTGTGCTGGGCGGCTGGCGACCAGCAAGCGCGTG > Exported/109483‑109620 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |