| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 954,082 | A→C | 5.4% | I504S (ATT→AGT) | gadC ← | predicted glutamate:gamma‑aminobutyric acid antiporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 954,082 | 0 | A | C | 5.4% | 490.9 / 18.3 | 165 | I504S (ATT→AGT) | gadC | predicted glutamate:gamma‑aminobutyric acid antiporter |
| Reads supporting (aligned to +/- strand): ref base A (83/73); new base C (2/7); total (85/80) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 9.15e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 184 total aligned reads displayed.
CGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTGCGTTCTGACTGTTGATTGG > Exported/954014‑954152 | cGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACGata < 1:3203568/71‑1 (MQ=255) aTGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAata > 1:2893125/1‑69 (MQ=255) tGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAg > 1:1049569/1‑71 (MQ=255) tGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAg > 1:491735/1‑71 (MQ=255) gAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAgt > 1:1500716/1‑71 (MQ=255) aaTTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCACCACACtatatt < 1:3376227/70‑3 (MQ=255) tATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTgtgg > 1:713540/1‑71 (MQ=255) aTCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTgtggt > 1:2081062/1‑71 (MQ=255) aTCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTgtggt > 1:1158985/1‑71 (MQ=255) gCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGa < 1:3262181/70‑1 (MQ=255) cTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAAc < 1:2949629/71‑1 (MQ=255) cTCCCTTGTCTCATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAAc < 1:530336/71‑1 (MQ=255) cccTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGt > 1:2383579/1‑71 (MQ=255) cccTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGt > 1:3441914/1‑71 (MQ=255) ccTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGt < 1:751160/70‑1 (MQ=255) ccTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCAACACAATATAGTGTGGTGAACGTg > 1:2156579/1‑71 (MQ=255) cTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGc < 1:1641975/71‑1 (MQ=255) cTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGc < 1:1268171/71‑1 (MQ=255) tGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAgt > 1:1290871/1‑56 (MQ=255) tGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTgtggt > 1:1447389/1‑61 (MQ=255) tGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTgtggt > 1:960302/1‑61 (MQ=255) tGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCAc > 1:595099/1‑71 (MQ=255) gTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCAcg > 1:1412473/1‑71 (MQ=255) gTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCAcg > 1:781068/1‑71 (MQ=255) gTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCAcg > 1:1024719/1‑71 (MQ=255) gTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCAcg > 1:1724097/1‑71 (MQ=255) tCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTgtgg > 1:942989/1‑58 (MQ=255) tCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCAcg < 1:1124865/70‑1 (MQ=255) ataACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACaa > 1:1799774/1‑44 (MQ=255) ataACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACaa > 1:1140836/1‑44 (MQ=255) ataACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACaa > 1:2139271/1‑44 (MQ=255) ataACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACaa > 1:2200116/1‑44 (MQ=255) ataACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAgtg > 1:2132425/1‑51 (MQ=255) ataACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCAcgcg < 1:1902106/68‑1 (MQ=255) taACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTg < 1:2824976/71‑1 (MQ=255) aaCCATTCATACATGGTTAGTGTTTCTTGTCATTCACCACACTATATtgtgat < 1:3159158/53‑3 (MQ=21) ccATTCAGACATGGTTAGTGTTTCTTGTCATTCACCACACTATATTgtgatg > 1:1217438/1‑52 (MQ=25) cAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGc > 1:3079348/1‑54 (MQ=255) cAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGc > 1:2587894/1‑54 (MQ=255) cAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGgaag > 1:996416/1‑70 (MQ=255) cAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGgaag > 1:2522012/1‑70 (MQ=255) cAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGgaag < 1:2846454/70‑1 (MQ=255) cAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGgaag > 1:394823/1‑70 (MQ=255) cAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGgaag < 1:1613435/70‑1 (MQ=255) cAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGgaag > 1:1651339/1‑70 (MQ=255) cAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGgaag > 1:1191265/1‑70 (MQ=255) cAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGgaag < 1:1952245/70‑1 (MQ=255) gACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGaagaag < 1:2184892/71‑1 (MQ=255) gACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGaagaag < 1:830777/71‑1 (MQ=255) cATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGt > 1:859293/1‑70 (MQ=255) aaTGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGaagaag < 1:638681/68‑1 (MQ=255) aaTGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGaagaag < 1:18100/68‑1 (MQ=255) aTGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCa > 1:2021853/1‑50 (MQ=255) aTGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCa > 1:2981766/1‑50 (MQ=255) aTGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCa > 1:1839870/1‑50 (MQ=255) aTGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCa > 1:1242299/1‑50 (MQ=255) aTGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGa > 1:3141989/1‑71 (MQ=255) aTGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGa > 1:2691548/1‑71 (MQ=255) aGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCttt < 1:1388746/70‑1 (MQ=255) gtgtTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTg > 1:3274778/1‑71 (MQ=255) gtgtTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTg < 1:157635/71‑1 (MQ=255) gtgtTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTg > 1:2456537/1‑71 (MQ=255) gtgtTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTg < 1:1762758/71‑1 (MQ=255) gtgtTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTg < 1:1311273/71‑1 (MQ=255) gtgtTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTg < 1:2276749/71‑1 (MQ=255) gtgtTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTg < 1:1686052/71‑1 (MQ=255) tgtTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTgg < 1:1980019/71‑1 (MQ=255) tgtTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTgg < 1:1981168/71‑1 (MQ=255) tttCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTgg > 1:308453/1‑69 (MQ=255) tttCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGt < 1:2379792/70‑1 (MQ=255) ttCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTg < 1:2055081/70‑1 (MQ=255) tCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCtttt > 1:295351/1‑65 (MQ=255) tCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCtttt > 1:1925128/1‑65 (MQ=255) tCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTGCg > 1:65449/1‑71 (MQ=255) tGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTgg > 1:508715/1‑64 (MQ=255) gTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTGCGtt < 1:2030985/69‑1 (MQ=255) gTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTGCGtt < 1:2809225/69‑1 (MQ=255) gTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTGCGTTc < 1:240204/70‑1 (MQ=255) tcattcatCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTGCGTTCt < 1:695939/70‑1 (MQ=255) tcattcatCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTGCGTTCt < 1:1073765/70‑1 (MQ=255) tcattcatCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTGCGTTCTg < 1:3238450/71‑1 (MQ=255) cattcatCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTGCGTTCTg < 1:43648/70‑1 (MQ=255) attcatCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCtttt > 1:776880/1‑58 (MQ=255) attcatCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTGCg < 1:1854005/64‑1 (MQ=255) attcatCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTGCg < 1:1485001/64‑1 (MQ=255) ttcatCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTGCGTTCTGACt > 1:2233249/1‑71 (MQ=255) tcatcaCAATTTAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTGCGTTCTGACTg > 1:1638606/1‑71 (MQ=255) tcatcaCAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTGCGtt > 1:2479346/1‑64 (MQ=255) tcatcaCAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTGCGtt > 1:908013/1‑64 (MQ=255) tcatcaCAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTGCGTTCTGACt > 1:2919565/1‑70 (MQ=255) tcatcaCAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTGCGTTCTGACTg > 1:1357490/1‑71 (MQ=255) atcaCAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTGCGTTCTGACTGtt < 1:3448431/71‑1 (MQ=255) atcaCAATATAGTGTGGTGAACGTGCACGCGGGGGCAGGAAGAAGTGACCTTTTGGTGCGTTCTGACTGtt < 1:3014066/71‑1 (MQ=255) tcaCAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTGCGTTCTGACTGTTg < 1:1058639/71‑1 (MQ=255) tcaCAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTGCGTTCTGACTGTTg < 1:1439616/71‑1 (MQ=255) cacaATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCttt < 1:1006102/51‑1 (MQ=255) cacaATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCttt < 1:1145788/51‑1 (MQ=255) caATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTGCGTTCTGACTGTTGAtt < 1:3211163/71‑1 (MQ=255) atatAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTGCGTTCTGACTGTTGATTgg < 1:2968126/71‑1 (MQ=255) atatAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTGCGTTCTGACTGTTGATTgg < 1:43503/71‑1 (MQ=255) | CGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTGCGTTCTGACTGTTGATTGG > Exported/954014‑954152 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |