Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 1,763,594 A→T 10.9% noncoding (250/1542 nt) rrsG ← 16S ribosomal RNA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported1,763,5940AT10.9% 239.6 / 16.1 82noncoding (250/1542 nt)rrsG16S ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base A (35/38);  new base T (4/5);  total (39/43)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCAGTGTGGCTGGTCATCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAAGGTCCCCCTCTTTGGTCTTGCGACA  >  Exported/1763525‑1763661
                                                                     |                                                                   
ccAGTGTGGCTGGTCATCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACcaaca                                                                     <  1:1784568/70‑1 (MQ=255)
ccAGTGTGGCTGGTCATCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACcaaca                                                                     <  1:2534359/70‑1 (MQ=255)
ccAGTGTGGCTGGTCATCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACcaaca                                                                     <  1:2341536/70‑1 (MQ=255)
    tgtgGCTGGTCATCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTa                                                                <  1:123096/71‑1 (MQ=255)
    tgtgGCTGGTCATCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTa                                                                <  1:3391515/71‑1 (MQ=255)
    tgtgGCTGGTCATCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTa                                                                <  1:2716738/71‑1 (MQ=255)
    tgtgGCTGGTCATCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTa                                                                <  1:203697/71‑1 (MQ=255)
    tgtgGCTGGTCATCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCATTACCCCACCAACAAGCTa                                                                <  1:1654433/71‑1 (MQ=255)
      tgGCTGGTCATCCTCTCAGACCAGCCAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTaa                                                               <  1:2333982/70‑1 (MQ=255)
      tgGCTGGTCATCCTCTCAGACCAGCCAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAAt                                                              <  1:2620077/71‑1 (MQ=255)
        gCTGGTCATCCTCTCAGACCAGCCAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATc                                                             <  1:736120/70‑1 (MQ=255)
        gCTGGTCATCCTCTCAGACCAGCCAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATc                                                             <  1:2296588/70‑1 (MQ=255)
          tGGTCGTCCTCTCAGACCAGCTACTGATCGTCGCCTTGGTGAGCCTTTACCTCACCAACTAGCTAAt                                                              >  1:42192/1‑67 (MQ=11)
            atCATCCTCTCAGACCAGTTACGGATCGTCGCCTTGGTAGGCCTTTACCCCACCAACTAGCTAATccga                                                          <  1:1856091/68‑3 (MQ=11)
             tCATCCTCTCAGACCAGCCAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCt                                                       <  1:2828359/71‑1 (MQ=255)
                tCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTggg                                                    >  1:3225207/1‑71 (MQ=255)
                tCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTggg                                                    >  1:1322170/1‑71 (MQ=255)
                tCCTCTCAGACCAGCCAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTgg                                                     <  1:3165672/70‑1 (MQ=255)
                   tctcAGACCAGCCAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGca                                                  >  1:257865/1‑70 (MQ=255)
                    ctcAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGc                                                                  >  1:2983573/1‑53 (MQ=255)
                    ctcAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGc                                                                  >  1:2533351/1‑53 (MQ=255)
                      cAGACCAGCCAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCt                                                       >  1:3144855/1‑62 (MQ=255)
                      cAGACCAGCCAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCt                                                       >  1:770004/1‑62 (MQ=255)
                       aGACCAGCTAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACAt                                               <  1:3278459/69‑1 (MQ=255)
                       aGACCAGCTACTGATCGTCGCCTTGGTGAGCCTTTACCTCACCAACTAGCTaa                                                               <  1:2266679/53‑1 (MQ=11)
                       aGACCAGCTACTGATCGTCGCCTTGGTGAGCCTTTACCTCACCAACTAGCTaa                                                               <  1:2262613/53‑1 (MQ=11)
                       aGACCAGCTACTGATCGTCGCCTTGGTGAGCCTTTACCTCACCAACTAGCTaa                                                               <  1:359788/53‑1 (MQ=11)
                       aGACCAGCTACTGATCGTCGCCTTGGTGAGCCTTTACCTCACCAACTAGCTaa                                                               <  1:662917/53‑1 (MQ=11)
                         aCCAGCCAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGa                                           <  1:2777904/71‑1 (MQ=255)
                          ccAGCTAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGAt                                          >  1:298506/1‑71 (MQ=255)
                            aGCCAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATgg                                        <  1:1970495/71‑1 (MQ=255)
                             gccaGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGc                                       <  1:2416041/68‑1 (MQ=255)
                             gccaGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGc                                       <  1:873395/68‑1 (MQ=255)
                              cTAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCa                                      <  1:2564884/71‑1 (MQ=255)
                                acggATCGTCGCCTTGGTAGGCCTTTACCCCACCAACTAGCt                                                                 >  1:1614449/3‑42 (MQ=14)
                                 gggATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCt                                                                 <  1:1278351/41‑1 (MQ=255)
                                 gggATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCaa                                     <  1:3391713/69‑1 (MQ=255)
                                 gggATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCaa                                     <  1:1683061/69‑1 (MQ=255)
                                  ggATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATcc                                             <  1:1444325/60‑1 (MQ=255)
                                  ggATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATcc                                             <  1:2549819/60‑1 (MQ=255)
                                  ggATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATcc                                             <  1:2654497/60‑1 (MQ=255)
                                  ggATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAagag                                  >  1:2379663/1‑71 (MQ=255)
                                   gATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATc                                                        <  1:648730/48‑1 (MQ=255)
                                   gATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATc                                                        <  1:1253892/48‑1 (MQ=255)
                                   gATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATc                                                        <  1:1874019/48‑1 (MQ=255)
                                   gATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAgg                                 <  1:1173309/71‑1 (MQ=255)
                                     tcgtcgCCTTGCTGAGCCGTTACCCCACCAACAAGCTAATCCCAt                                                         >  1:1478389/1‑45 (MQ=255)
                                      cgtcgCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGccc                              >  1:620598/1‑71 (MQ=255)
                                       gtcgCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGccc                              >  1:1554427/1‑70 (MQ=255)
                                        tcgCCTTGGTGAGCCGTTACCCCACCAACAAGCTaa                                                               >  1:816819/1‑36 (MQ=255)
                                        tcgCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGa                            <  1:484548/71‑1 (MQ=255)
                                        tcgCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGa                            <  1:1551600/71‑1 (MQ=255)
                                         cgCCTTGGTAGGCCTTTACCCCACCAACTAGCTAATCCGACCTAGGCTCAt                                               >  1:2969043/1‑51 (MQ=2)
                                         cgCCTTGGTAGGCCTTTACCCCACCAACTAGCTAATCCGACCTAGGCTCAt                                               >  1:1053903/1‑51 (MQ=2)
                                          gCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGagg                          >  1:2661987/1‑69 (MQ=255)
                                             ttGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAAGGTc                       >  1:3403957/1‑71 (MQ=255)
                                              tGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAGGGTcc                      >  1:391732/1‑71 (MQ=255)
                                              tGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAGGGTcc                      >  1:1870370/1‑71 (MQ=255)
                                              tGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAGGGTcc                      >  1:3468082/1‑71 (MQ=255)
                                               ggTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAGGGTccc                     >  1:805235/1‑71 (MQ=255)
                                                   aGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAAGGTCCCCct                  <  1:1641785/70‑1 (MQ=40)
                                                    gCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAGGGTCCCCctct                >  1:339240/1‑71 (MQ=255)
                                                     ccGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATcc                                             <  1:159787/41‑1 (MQ=255)
                                                     ccGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATcc                                             <  1:2339024/41‑1 (MQ=255)
                                                       gTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAGGGTCCCCCTCTTTg             >  1:1700882/1‑71 (MQ=255)
                                                       gTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAAGGTCCCCCTCTTTg             <  1:246218/71‑1 (MQ=39)
                                                       gTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAAGGTCCCCCTCTTTg             <  1:2076713/71‑1 (MQ=39)
                                                       gTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAAGGTCCCCCTCTTTg             <  1:1127441/71‑1 (MQ=39)
                                                       gTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAAGGTCCCCCTCTTTg             <  1:1753927/71‑1 (MQ=39)
                                                       gTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAACAGGCCCGAGGGTCCCCCTCTTTg             <  1:2876121/71‑1 (MQ=255)
                                                        ttACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAGGGTCCCCCTCtt               <  1:3022563/68‑1 (MQ=255)
                                                          aCCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAGGGTCCCCCTCTTTGGTc          >  1:579793/1‑71 (MQ=255)
                                                          aCCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAAGGTcccc                    >  1:3419809/1‑61 (MQ=40)
                                                          aCCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAAGGTcccc                    >  1:613810/1‑61 (MQ=40)
                                                          aCCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAAGGTCCCCCTCTTTGGTc          >  1:1888695/1‑71 (MQ=39)
                                                            cccACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAGGGTCCCCCTCTTTGGTCtt        >  1:822803/1‑71 (MQ=255)
                                                              caccaACAAGCTAATCCCATCTGGGCACATCCGATGGCAaga                                   >  1:2083765/1‑42 (MQ=255)
                                                              caccaACAAGCTAATCCCATCTGGGCACATCCGATGGCAaga                                   >  1:1798974/1‑42 (MQ=255)
                                                              caccaACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAGGGTCCCCCTCTTTGGTc          >  1:2088287/1‑67 (MQ=255)
                                                              caccaACAAGCTAATCCCATCTGGGAACATCCGATGGCAaga                                   >  1:666952/1‑42 (MQ=255)
                                                                ccaACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAGGGTCCCCctct                >  1:1516373/1‑59 (MQ=255)
                                                                ccaACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAGGGTCCCCCTCTTTGGTCTTGCGa    >  1:710359/1‑71 (MQ=255)
                                                                ccaACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAGGGTCCCCCTCTTTGGTCTTGCGa    >  1:726720/1‑71 (MQ=255)
                                                                ccaACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAGGGTCCCCCTCTTTGGTCTTGCGa    >  1:789797/1‑71 (MQ=255)
                                                                ccaACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAAGGTCCCCCTCTTTGGTCTTGCGa    >  1:463062/1‑71 (MQ=39)
                                                                 caacaaGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAAGGTCCCCCTCTTTGGTCTTGCGa    >  1:1245183/1‑70 (MQ=39)
                                                                 caacaaGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAAGGTCCCCCTCTTTGGTCTTGCGa    >  1:2814158/1‑70 (MQ=39)
                                                                  aacaaGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAGGGTCCCCCTCTTTGGTCTTGCGAcg  >  1:1275101/1‑70 (MQ=19)
                                                                   acaaGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAGGGTCCCCCTCTTTGGTCTTGCg     >  1:3090541/1‑67 (MQ=25)
                                                                     |                                                                   
CCAGTGTGGCTGGTCATCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAAGGTCCCCCTCTTTGGTCTTGCGACA  >  Exported/1763525‑1763661

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑