Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	Exported	1,763,636	A→G	35.4%	noncoding (208/1542 nt)	rrsG ←	16S ribosomal RNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	Exported	1,763,636	0	A	G	35.4%	44.7 / 46.4	48	noncoding (208/1542 nt)	rrsG	16S ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base A (18/13);  new base G (15/2);  total (33/15)
Fisher's exact test for biased strand distribution p-value = 4.98e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.13e-01

TTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAAGGTCCCCCTCTTTGGTCTTGCGACATTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTC  >  Exported/1763570‑1763702                                                                   |                                                                   ttGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAAGGTc                                                                >  1:3403957/1‑71 (MQ=255)  tGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAGGGTcc                                                               >  1:391732/1‑71 (MQ=255)  tGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAGGGTcc                                                               >  1:3468082/1‑71 (MQ=255)  tGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAGGGTcc                                                               >  1:1870370/1‑71 (MQ=255)   ggTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAGGGTccc                                                              >  1:805235/1‑71 (MQ=255)       aGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAAGGTCCCCct                                                           <  1:1641785/70‑1 (MQ=40)        gCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAGGGTCCCCctct                                                         >  1:339240/1‑71 (MQ=255)           gTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAGGGTCCCCCTCTTTg                                                      >  1:1700882/1‑71 (MQ=255)           gTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAAGGTCCCCCTCTTTg                                                      <  1:1753927/71‑1 (MQ=39)           gTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAAGGTCCCCCTCTTTg                                                      <  1:2076713/71‑1 (MQ=39)           gTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAAGGTCCCCCTCTTTg                                                      <  1:246218/71‑1 (MQ=39)           gTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAAGGTCCCCCTCTTTg                                                      <  1:1127441/71‑1 (MQ=39)           gTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAACAGGCCCGAGGGTCCCCCTCTTTg                                                      <  1:2876121/71‑1 (MQ=255)            ttACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAGGGTCCCCCTCtt                                                        <  1:3022563/68‑1 (MQ=255)              aCCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAGGGTCCCCCTCTTTGGTc                                                   >  1:579793/1‑71 (MQ=255)              aCCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAAGGTcccc                                                             >  1:3419809/1‑61 (MQ=40)              aCCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAAGGTcccc                                                             >  1:613810/1‑61 (MQ=40)              aCCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAAGGTCCCCCTCTTTGGTc                                                   >  1:1888695/1‑71 (MQ=39)                cccACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAGGGTCCCCCTCTTTGGTCtt                                                 >  1:822803/1‑71 (MQ=255)                  caccaACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAGGGTCCCCCTCTTTGGTc                                                   >  1:2088287/1‑67 (MQ=255)                    ccaACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAGGGTCCCCctct                                                         >  1:1516373/1‑59 (MQ=255)                    ccaACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAGGGTCCCCCTCTTTGGTCTTGCGa                                             >  1:789797/1‑71 (MQ=255)                    ccaACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAGGGTCCCCCTCTTTGGTCTTGCGa                                             >  1:710359/1‑71 (MQ=255)                    ccaACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAGGGTCCCCCTCTTTGGTCTTGCGa                                             >  1:726720/1‑71 (MQ=255)                    ccaACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAAGGTCCCCCTCTTTGGTCTTGCGa                                             >  1:463062/1‑71 (MQ=39)                     caacaaGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAAGGTCCCCCTCTTTGGTCTTGCGa                                             >  1:2814158/1‑70 (MQ=39)                     caacaaGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAAGGTCCCCCTCTTTGGTCTTGCGa                                             >  1:1245183/1‑70 (MQ=39)                      aacaaGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAGGGTCCCCCTCTTTGGTCTTGCGAcg                                           >  1:1275101/1‑70 (MQ=19)                       acaaGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAGGGTCCCCCTCTTTGGTCTTGCg                                              >  1:3090541/1‑67 (MQ=25)                           gCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAAGGTCCCCCTCTTTGGTCTTGCGACATTATg                                      <  1:749994/71‑1 (MQ=34)                           gCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAAGGTCCCCCTCTTTGGTCTTGCGACATTATg                                      <  1:72191/71‑1 (MQ=34)                                  ccATCTGGGCACATCCGATGGCAAGAGGCCCGAAGGTCCCCCTCTTTGGTCTTGCGACATTATGCGGTAt                                >  1:3316063/1‑70 (MQ=35)                                   cATCTGGGCACATCCGATGGCAAGAGGCCCGAAGGTCCCCCTCTTTGGTCTTGCGACATTATGCGGTAt                                >  1:1013593/1‑69 (MQ=35)                                    aTCTGGGCACATCCGATGGCAAGAGGCCCGAAGGTCCCCCTCTTTGGTCTTGCGACATTATGCGGTATTAg                             <  1:1398915/71‑1 (MQ=34)                                    aTCTGGGCACATCCGATGGCAAGAGGCCCGAAGGTCCCCCTCTTTGGTCTTGCGACATTATGCGGTATTAg                             <  1:121561/71‑1 (MQ=34)                                         ggCACATCCGATGGCAAGAGGCCCGAAGGTCCCCCTCTTTGGTCTTGCGACATTATGCGGTATTAGCt                           <  1:1103168/68‑1 (MQ=35)                                           cacaTCCGATGGCAAGAGGCCCGAAGGTCCCCCTCTTTGGTCTTGCGACATTATGCGGTATTAGCTACCGt                      >  1:79959/1‑71 (MQ=34)                                            acaTCCGATGGCAAGAGGCCCGAAGGTCCCCCTCTTTGGTCTTGCGACATTATGCGGTATTAGCTACCGtt                     <  1:3385696/71‑1 (MQ=34)                                              aTCCGATGGCAAGAGGCCCGAAGGTCCCCCTCTTTGGTCTTGCGACATTATGCGGTATTAg                             <  1:3187124/61‑1 (MQ=35)                                               tCCGATGGCAAGCGGCCCGAAGGTCCCCCTCTTTGGTCTTGCGACATTATGCGGTATTAGCTACCGttt                    >  1:2065739/1‑69 (MQ=25)                                               tCCGATGGCAAGAGGCCCGAAGGTCCCCCTCTTTGGTCTTGCGACATTATGCGGTATTAGCTACCGTTTc                   >  1:658135/1‑70 (MQ=35)                                               tCCGATGGCAAGAGGCCCGAAGGTCCCCCTCTTTGGTCTTGCGACATTATGCGGTATTAGCTACCGTTTc                   >  1:1451883/1‑70 (MQ=35)                                                 cGATGGCAAGAGGCCCGAAGGTCCCCCTCTTTGGTCTTGCGACATTATGCGGTATTAg                             >  1:2900098/1‑58 (MQ=35)                                                  gATGGCAAGAGGCCCGAAGGTCCCCCTCTTTGGTCTTGCGACATTATGCGGTATTAGCTACCGTTTCCag                >  1:1580791/1‑70 (MQ=35)                                                     ggCAAGAGGCCCGAAGGTCCCCCTCTTTGGTCTTGCGACATTATGCGGTATTAGCTACCGTTTCCagtagt            >  1:29751/1‑71 (MQ=34)                                                           agGCCCGAAGGTCCCCCTCTTTGGTCTTGCGACATTATGCGGTATTAg                             <  1:2144391/48‑1 (MQ=38)                                                           agGCCCGAAGGTCCCCCTCTTTGGTCTTGCGACATTATGCGGTATTAGCTACCGTTTCCAGTAGTTATccc      >  1:2647732/1‑71 (MQ=34)                                                               ccGAAGGTCCCCCTCTTTGGTCTTGCGACATTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTc  >  1:2468477/1‑71 (MQ=34)                                                                   |                                                                   TTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAAGGTCCCCCTCTTTGGTCTTGCGACATTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTC  >  Exported/1763570‑1763702

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑