| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 2,255,098 | G→A | 7.7% | P647L (CCG→CTG) | rpoC ← | RNA polymerase, beta prime subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 2,255,098 | 0 | G | A | 7.7% | 205.3 / 6.4 | 65 | P647L (CCG→CTG) | rpoC | RNA polymerase, beta prime subunit |
| Reads supporting (aligned to +/- strand): ref base G (25/35); new base A (3/2); total (28/37) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.44e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.12e-01 | |||||||||||
GACTGGAACTGCTCCTGAATTTCAGCAACTTCTGCTTCTGCCTCGGAGATGATTTCGTGTTTCTTCTCCGGGATGACCATGTCATCGATACCAACAGATGCACCAGAACGCGCTGCATAGGCGAAGCCGGTGTACATG > Exported/2255029‑2255166 | gacTGGAACTGCTCCTGAATTTCAGCAACTTCTGCTTCTGCCTCGGAGATGATTTCGTGTTTCTTCTCCgg > 1:3811/1‑71 (MQ=255) tGGAACTGCTCCTGAATTTCAGCAACTTCTGCTTCTGCCTCGGAGATGATTTCGTGTTTCTTCTCCGGGAt > 1:2154696/1‑71 (MQ=255) tGGAACTGCTCCTGAATTTCAGCAACTTCTGCTTCTGCCTCGGAGATGATTTCGTGTTTCTTCTCCGGGAt > 1:752846/1‑71 (MQ=255) tGGAACTGCTCCTGAATTTCAGCAACTTCTGCTTCTGCCTCGGAGATGATTTCGTGTTTCTTCTCCGGGAt > 1:1407357/1‑71 (MQ=255) aaCTGCTCCTGAATTTCAGCAACTTCTGCTTCTGCCTCGGAGATGATTTCGTGTTTCTTCTCCgg < 1:1936310/65‑1 (MQ=255) aCTGCTCCTGAATTTCAGCAACTTCTGCTTCTGCCTCGGAGATGATTTCGTGTTTCTTCTCCGGGATGAcc < 1:226418/71‑1 (MQ=255) aCTGCTCCTGAATTTCAGCAACTTCTGCTTCTGCCTCGGAGATGATTTCGTGTTTCTTCTCCGGGATGAc > 1:2396318/1‑70 (MQ=255) cTCCTGAATTTCAGCAACTTCTGCTTCTGCCTCGGAGATGATTTCGTGTTTCTTCTCCGGGATGACCATGt < 1:1643979/71‑1 (MQ=255) cTGAATTTCAGCAACTTCTGCTTCTGCCTCGGAGATGATTTCGTGTTTCTTCTCCGGGATGACCATGTc < 1:1244229/69‑1 (MQ=255) cTGAATTTCAGCAACTTCTGCTTCTGCCTCGGAGATGATTTCGTGTGTCTTCTCCGGGATGACCATGTc < 1:2200537/69‑1 (MQ=255) tGAATTTCAGCAACTTCTGCTTCTGCCTCGGAGATGATTTCGTGTTTCTTCTCCgg < 1:2720776/56‑1 (MQ=255) aTTTCAGCAACTTCTGCTTCTGCCTCGGAGATGATTTCGTGTTTCTTCTCCGGGATGACCATGTCATCGAt < 1:542872/71‑1 (MQ=255) cAGCAACTTCTGCTTCTGCCTCGGAGATGATTTCGTGTTTCTTCTCCGGGATGACCATATCATCGATAcc < 1:1309580/70‑1 (MQ=255) aGCAACTTCTGCTTCTGCCTCGGAGATGATTTCGTGTTTCTTCTCCGGGATGACCATGTCATCGATACCa < 1:2413213/70‑1 (MQ=255) gCAACTTCTGCTTCTGCCTCGGAGATGATTTCGTGTTTCTTCTCCGGGATGACCATGTCATCGATACCAAc < 1:1720516/71‑1 (MQ=255) aaCTTCTGCTTCTGCCTCGGAGATGATTTCGTGTTTCTTCTCCGGGATGACCATGTCATCGa < 1:1586035/62‑1 (MQ=255) aaCTTCTGCTTCTGCCTCGGAGATGATTTCGTGTTTCTTCTCCGGGATGACCATGTCATCGa < 1:3392659/62‑1 (MQ=255) aaCTTCTGCTTCTGCCTCGGAGATGATTTCGTGTTTCTTCTCCGGGATGACCATGTCATCGa < 1:1768693/62‑1 (MQ=255) aaCTTCTGCTTCTGCCTCGGAGATGATTTCGTGTTTCTTCTCCGGGATGACCATGTCATCGa < 1:1917864/62‑1 (MQ=255) aaCTTCTGCTTCTGCCTCGGAGATGATTTCGTGTTTCTTCTCCGGGATGACCATGTCATCGATACCaa > 1:1225643/1‑68 (MQ=255) ctgcttctgcCTCGGAGATGATTTCGTGTTTCTTCTCCGGGATGACCATGTCATCGATACCAACAGATGc < 1:2591086/70‑1 (MQ=255) ctgcttctgcCTCGGAGATGATTTCGTGTTTCTTCTCCGGGATGACCATGTCATCGATACCAACAGATGCa > 1:689517/1‑71 (MQ=255) ctgcttctgcCTCGGAGATGATTTCGTGTTTCTTCTCCGGGATGACCATGTCATCGATACCAACAGATGCa > 1:2206753/1‑71 (MQ=255) ctgcttctgcCTCGGAGATGATTTCGTGTTTCTTCTCCGGGATGACCATGTCATCGATACCAACAGATGCa > 1:2612955/1‑71 (MQ=255) ctgcttctgcCTCGGAGATGATTTCGTCTTTCTTCTCCGGGATGACCATGTCATCGATACCAACAGATGCa > 1:1340997/1‑71 (MQ=255) tgcttctgcCTCGGAGATGATTTCGTGTTTCTTCTCCGGGa > 1:2916863/1‑41 (MQ=255) gcttctgcCTCGGAGATGATTTCGTGTTTCTTCTCCGGGATGACCATGTCATCGATACCAACAGATGCAc > 1:1024110/1‑70 (MQ=255) gcttctgcCTCGGAGATGATTTCGTGTTTCTTCTCCGGGATGACCATGTCATCGATACCAACAGATGCAc > 1:1210356/1‑70 (MQ=255) ccTCGGAGATGATTTCGTGTTTCTTCTCCGGGATGACCATGTCATCGATACCAACAGATGCACCAGAAcgc < 1:300712/71‑1 (MQ=255) ggAGATGATTTCGTGTTTCTTCTCCGGGATGACCATGTCATCGATACCAACAGa > 1:2542537/1‑54 (MQ=255) ggAGATGATTTCGTGTTTCTTCTCCGGGATGACCATGTCATCGATACCAACAGa > 1:1180507/1‑54 (MQ=255) ggAGATGATTTCGTGTTTCTTCTCCGGGATGACCATGTCATCGATACCAACAGATGCACCAGAACGCGCTg < 1:3339319/71‑1 (MQ=255) gagaTGATTTCGTGTTTCTTCTCCGGGATGACCATGTCATc < 1:211057/41‑1 (MQ=255) gagaTGATTTCGTGTTTCTTCTCCGGGATGACCATGTCATc < 1:984707/41‑1 (MQ=255) gatgatTTCGTGTTTCTTCTCCGGGATGACCATGTCATCGATACCAACAGATGCACCAGAACGCGCTGCa > 1:2880833/1‑70 (MQ=255) gatgatTTCGTGTTTCTTCTCCGGGATGACCATGTCATCGATACCAACAGATGCACCAGAACGCGCTGCa > 1:3347902/1‑70 (MQ=255) gatgatTTCGTGTTTCTTCTCCGGGATGACCATGTCATCGATACCAACAGATGCACCAGAACGCGCTGCAt < 1:3239876/71‑1 (MQ=255) gatgatTTCGTGTTTCTTCTCCGGGATGACCATGTCATCGATACCAACAGATGCACCAGAACGCGCTGCAt < 1:590662/71‑1 (MQ=255) atgatTTCGTGTTTCTTCTCCGGGATGACCATGTCATCGATACCaa < 1:3326271/46‑1 (MQ=255) atgatTTCGTGTTTCTTCTCCGGGATGACCATGTCATCGATACCaa < 1:994329/46‑1 (MQ=255) atgatTTCGTGTTTCTTCTCCGGGATGACCATGTCATCGATACCaa < 1:1636332/46‑1 (MQ=255) atgatTTCGTGTTTCTTCTCCGGGATGACCATGTCATCGATACCAACAGATGCACCAGAACGCGCt > 1:400302/1‑66 (MQ=255) atgatTTCGTGTTTCTTCTCCGGGATGACCATGTCATCGATACCAACAGATGCACCAGAACGCGCTGCAt < 1:1152975/70‑1 (MQ=255) atgatTTCGTGTTTCTTCTCCGGGATGACCATGTCATCGATACCAACAGATGCACCAGAACGCGCTGCATa < 1:2215439/71‑1 (MQ=255) tgatTTCGTGTTTCTTCTCCAGGATGACCATGTCATCGATACCAACAGATGCACCAGa < 1:920031/58‑1 (MQ=255) tgatTTCGTGTTTCTTCTCCAGGATGACCATGTCATCGATACCAACAGATGCACCAGa < 1:575001/58‑1 (MQ=255) gatTTCGTGTTTCTTCTCCGGGATGACCATGTCATCGATAc > 1:2616099/1‑41 (MQ=255) gatTTCGTGTTTCTTCTCCGGGATGACCATGTCATCGATACCAACAGATGCACCAGAACGCGCTGCATAgg > 1:2903479/1‑71 (MQ=255) atTTCGTGTTTCTTCTCCGGGATGACCATGTCATCGATACCAACAGATGCACCAGAACGCGCTGCATAGGc > 1:1055658/1‑71 (MQ=255) atTTCGTGTTTCTTCTCCGGGATGACCATGTCATCGATACCAACAGATGCACCAGAACGCGCTGCATAGGc > 1:2913860/1‑71 (MQ=255) atTTCGTGTTTCTTCTCCAGGATGACCATGTCATCGATACCAACAGATGCACCAGAAc > 1:275265/1‑58 (MQ=255) atTTCGTGTTTCTTCTCCAGGATGACCATGTCATCGATACCAACAGATGCACCAGAAc > 1:2005206/1‑58 (MQ=255) atTTCGTGTTTCTTCTCCAGGATGACCATGTCATCGATACCAACAGATGCACCAGAAc > 1:3310040/1‑58 (MQ=255) tttCGTGTTTCTTCTCCGGGATGACCATGTCATCGATACCAACAGATGCACCAGAACGCGCTGCATAGGCg < 1:1226607/71‑1 (MQ=255) ttCGTGTTTCTTCTCCGGGATGACCATGTCATCGa < 1:1726775/35‑1 (MQ=255) ttCGTGTTTCTTCTCCGGGATGACCATGTCATCGa < 1:1491470/35‑1 (MQ=255) ttCGTGTTTCTTCTCCGGGATGACCATGTCATCGa < 1:2676414/35‑1 (MQ=255) tCGTGTTTCTTCTCCGGGATGACCATGTCATCGATACCAACAGAt > 1:2623742/1‑45 (MQ=255) tCGTGTTTCTTCTCCGGGATGACCATGTCATCGATACCAACAGATGCACCAGAACGCGCTGCATAGGCGa < 1:2212466/70‑1 (MQ=255) cGTGTTTCTTCTCCGGGATGACCATGTCATCGATACCAACAGATGCACCAGAACGCGCTGCATAGGCGaa < 1:1765327/70‑1 (MQ=255) tttCTTCTCCGGGATGACCATGTCATCGATACCAACAGATGCACCAGAACGCGCTGCATAGGCGAAGCCg < 1:179049/70‑1 (MQ=255) tttCTTCTCCGGGATGACCATGTCATCGATACCAACAGATGCACCAGAACGCGCTGCATAGGCGAAGCCg < 1:308584/70‑1 (MQ=255) ctcCGGGATGACCATGTCATCGATACCAACAGATGCACCAGAACGCGCTGCATAGGCGAAGCCGGTGTa < 1:1450738/69‑1 (MQ=255) ctcCGGGATGACCATGTCATCGATACCAACAGATGCACCAGAACGCGCTGCATAGGCGAAGCCGGTGTACa > 1:1668692/1‑71 (MQ=255) ctcCGGGATGACCATGTCATCGATACCAACAGATGCACCAGAACGCGCTGCATAGGCGAAGCCGGTGTACa > 1:1208614/1‑71 (MQ=255) ctcCGGGATGACCATGTCATCGATACCAACAGATGCACCAGAACGCGCTGCATAGGCGAAGCCGGTGTACa > 1:1171809/1‑71 (MQ=255) cGGGATGACCATGTCATCGATACCAACAGATGCACCAGAACGCGCTGCATAGGCGAAGCCGGTGTACATg < 1:572838/70‑1 (MQ=255) cGGGATGACCATGTCATCGATACCAACAGATGCACCAGAACGCGCTGCATAGGCGAAGCCGGTGTACATg < 1:2882091/70‑1 (MQ=255) | GACTGGAACTGCTCCTGAATTTCAGCAACTTCTGCTTCTGCCTCGGAGATGATTTCGTGTTTCTTCTCCGGGATGACCATGTCATCGATACCAACAGATGCACCAGAACGCGCTGCATAGGCGAAGCCGGTGTACATG > Exported/2255029‑2255166 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |