Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 2,469,906 A→C 17.6% A296A (GCA→GCC atpD → F1 sector of membrane‑bound ATP synthase, beta subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported2,469,9060AC17.6% 134.6 / 33.0 68A296A (GCA→GCCatpDF1 sector of membrane‑bound ATP synthase, beta subunit
Reads supporting (aligned to +/- strand):  ref base A (26/30);  new base C (5/7);  total (31/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGAAGAGATGGGCGTTCTGCAGGAACGTATCACCTCCACCAAAACTGGTTCTATCACCTCCGTACAGGCAGTATACGTACCTGCGGATGACTTGACTGACCCGTCTCCGGCAACCACCTTTGCGCACCTTGACGCAACCG  >  Exported/2469837‑2469976
                                                                     |                                                                      
ggAAGAGATGGGCGTTCTGCAGGAACGTATCACCTCCACCAAAACTGGTTCTATCACCTCCGTACAGGCAg                                                                       <  1:1257744/71‑1 (MQ=255)
 gAAGAGATGGGCGTTCTGCAGGAACGTATCACCTCCACCAAAACTGGTTCTATCACCTCCGTACAGGCAg                                                                       <  1:1887403/70‑1 (MQ=255)
  aaGAGATGGGCGTTCTGCAGGAACGTATCACCTCCACCAAAACTGGTTCTATCACCTCCGTACAGGCAGta                                                                     <  1:861823/71‑1 (MQ=255)
   agagATGGGCGTTCTGCAGGAACGTATCACCTCCACCAAAACTGGTTCTATCACCTCCGTACAGGCAGta                                                                     <  1:1806405/70‑1 (MQ=255)
       aTGGGCGTTCTGCAGGAACGTATCACCTCCACCAAAACTGGTTCTATCACCTCCGTACAGGCAGTATACg                                                                 >  1:2949495/1‑70 (MQ=255)
       aTGGGCGTTCTGCAGGAACGTATCACCTCCACCAAAACTGGTTCTATCACCTCCGTACAGGCAGTATACg                                                                 >  1:3367624/1‑70 (MQ=255)
       aTGGGCGTTCTGCAGGAACGTATCACCTCCACCAAAACTGGTTCTATCACCTCCGTACAGGCAGTATACg                                                                 >  1:277513/1‑70 (MQ=255)
            cGTTCTGCAGGAACGTATCACCTCCACCAAAACTGGTTCTATCACCTCCGTACAGGCAGTATACGTACCt                                                            >  1:1155231/1‑70 (MQ=255)
            cGTTCTGCAGGAACGTATCACCTCCACCAAAACTGGTTCTATCACCTCCGTACAGGCAGTATACGTACCTg                                                           >  1:2782294/1‑71 (MQ=255)
              ttCTGCAGGAACGTATCACCTCCACCAAAACTGGTTCTATCACCTCCGTACAGGCAGTATACGTACCTGCg                                                         <  1:3397186/71‑1 (MQ=255)
              ttCTGCAGGAACGTATCACCTCCACCAAAACTGGTTCTATCACCTCCGTACAGGCAGTATACGTACCTGCg                                                         <  1:3401195/71‑1 (MQ=255)
                cTGCAGGAACGTATCACCTCCACCAAAACTGGTTCTATCACCTCCGTACAGGCAg                                                                       <  1:1209989/55‑1 (MQ=255)
                cTGCAGGAACGTATCACCTCCACCAAAACTGGTTCTATCACCTCCGTACAGGCAg                                                                       <  1:2051383/55‑1 (MQ=255)
                cTGCAGGAACGTATCACCTCCACCAAAACTGGTTCTATCACCTCCGTACAGGCAg                                                                       <  1:1734919/55‑1 (MQ=255)
                cTGCAGGAACGTATCACCTCCACCAAAACTGGTTCTATCACCTCCGTACAGGCAGTATACGTACCTGCGGa                                                       >  1:2044478/1‑71 (MQ=255)
                cTGCAGGAACGTATCACCTCCACCAAAACTGGTTCTATCACCTCCGTACAGGCAGTATACGTACCTGCGGa                                                       >  1:1775969/1‑71 (MQ=255)
                  gCAGGAACGTATCACCTCCACCAAAACTGGTTCTATCACCTCCGTACAGGCAGta                                                                     >  1:3258571/1‑55 (MQ=255)
                   cAGGAACGTATCACCTCCACCAAAACTGGTTCTATCACCTCCGTACAGGCAGTATACGTACCTGCGGAt                                                      <  1:602824/69‑1 (MQ=255)
                      gAACGTATCACCTCCACCAAAACTGGTTCTATCACCTCCGTACAGGCAGTATACGTACCTGCGGAtgactt                                                 <  1:1441752/71‑1 (MQ=255)
                      gAACGTATCACCTCCACCAAAACTGGTTCTATCACCTCCGTACAGGCAGTATACGTACCTGCGGAtgactt                                                 <  1:30089/71‑1 (MQ=255)
                      gAACGTATCACCTCCACCAAAACTGGTTCTATCACCTCCGTACAGGCAGTATACGTACCTGCGGAtgactt                                                 <  1:3045456/71‑1 (MQ=255)
                          gTATCACCTCCACCAAAACTGGTTCTATCACCTCCGTACAGGCAGTATACGTACCTGCGGAtgacttgact                                             <  1:1575890/71‑1 (MQ=255)
                          gTATCACCTCCACCAAAACTGGTTCTATCACCTCCGTACAGGCAGTATACGTACCTGCGGAtgacttga                                               >  1:830049/1‑69 (MQ=255)
                           tATCACCTCCACCAAAACTGGTTCTATCACCTCCGTACAGGCAGTATACGTACCTGCGGAtgacttgact                                             <  1:2947086/70‑1 (MQ=255)
                           tATCACCTCCACCAAAACTGGTTCTATCACCTCCGTACAGGCAGTATACGTACCTGCGGAtgacttgact                                             <  1:2384686/70‑1 (MQ=255)
                            aTCACCTCCACCAAAACTGGTTCTATCACCTCCGTACAGGCAGTATACGTACCTGCGGATGACTtgactga                                           >  1:1691340/1‑71 (MQ=255)
                             tCACCTCCACCAAGAAAGGCTCGATCACCTCGATCCAGGCCGTATACGttc                                                              <  1:2837128/51‑3 (MQ=255)
                             tCACCTCCACCAAGAAAGGCTCGATCACCTCGATCCAGGCCGTATACGttc                                                              <  1:357078/51‑3 (MQ=255)
                             tCACCTCCACCAAGAAAGGCTCGATCACCTCGATCCAGGCCGTATACGttc                                                              <  1:2320244/51‑3 (MQ=255)
                             tCACCTCCACCAAGAAAGGCTCGATCACCTCGATCCAGGCCGTATACGttc                                                              <  1:1522286/51‑3 (MQ=255)
                             tCACCTCCACCAAGAAAGGCTCGATCACCTCGATCCAGGCCGTATACGttc                                                              <  1:2273389/51‑3 (MQ=255)
                             tCACCTCCACCAAAACTGGTTCTATCACCTCCGTACAGGCAGTATACGTACCTGCGGATGACTtgactgac                                          >  1:101238/1‑71 (MQ=255)
                              cACCTCCACCAAAACTGGTTCTATCACCTCCGTACAGGCAGtat                                                                    >  1:3376782/1‑44 (MQ=255)
                              cACCTCCACCAAAACTGGTTCTATCACCTCCGTACAGGCAGtat                                                                    >  1:1618831/1‑44 (MQ=255)
                              cACCTCCACCAAAACTGGTTCTATCACCTCCGTACAGGCAGta                                                                     >  1:1589875/1‑43 (MQ=255)
                              cACCTCCACCAAAACTGGTTCTATCACCTCCGTACAGGCAGTATACGTACCTGCGGATGACTTGACTGAcc                                         >  1:904085/1‑71 (MQ=255)
                              cACCTCAACCAAAACTGGTTCTATCACCTCCGTACAGGCAGtat                                                                    >  1:1853/1‑44 (MQ=255)
                               aCCTCCACCAAGAAAGGCTCGATCACCTCGATCCAGGCCGTATACGTTccc                                                            >  1:1456628/1‑50 (MQ=255)
                               aCCTCCACCAAGAAAGGCTCGATCACCTCGATCCAGGCCGTATACGTTccc                                                            >  1:1656986/1‑50 (MQ=255)
                               aCCTCCACCAAGAAAGGCTCGATCACCTCGATCCAGGCCGTATACGTTccc                                                            >  1:807223/1‑50 (MQ=255)
                               aCCTCCACCAAGAAAGGCTCGATCACCTCGATCCAGGCCGTATACGTTccc                                                            >  1:1711370/1‑50 (MQ=255)
                               aCCTCCACCAAGAAAGGCTCGATCACCTCGAACCAGGCCGTATACGttc                                                              >  1:230767/1‑47 (MQ=255)
                                ccTCCACCAAAACTGGTTCTATCACCTCCGTACAGGCAGTATACGTAc                                                              >  1:847435/1‑48 (MQ=255)
                                ccTCCACCAAAACTGGTTCTATCACCTCCGTACAGGCAGTATACGTACCTGCGGATGACTTGACTGACCCg                                       <  1:1487376/71‑1 (MQ=255)
                                 cTCCACCAAAACTGGTTCTATCACCTCCGTACAGGCAGTATACGTACCTGCGGATGACTTGACTGACCCGt                                      <  1:939294/71‑1 (MQ=255)
                                    caccaAAACTGGTTCTATCACCTCCGTACAGGCAGTATACGTACCTGCGGATGACTTGACTGACCCGtctc                                   >  1:521784/1‑71 (MQ=255)
                                    caccaAAACTGGTTCTATCACCTCCGTACAGGCAGTATACGTACCTGCGGATGACTTGACTGACCCGtctc                                   >  1:2021175/1‑71 (MQ=255)
                                     accaAAACTGGTTCTATCACCTCCGTACAGGCAGTATACGTACCTGCGGATGACTTGACTGACCCGTCTcc                                  >  1:1149309/1‑71 (MQ=255)
                                     accaAAACTGGTTCTATCACCTCCGTACAGGCAGTATACGTACCTGCGGATGACTTGACTGACCCGTCTcc                                  >  1:1484170/1‑71 (MQ=255)
                                     accaAAACTGGTTCTATCACCTCCGTACAGGCAGTATACGTACCTGCGGATGACTTGACTGACCCGTCTcc                                  >  1:854831/1‑71 (MQ=255)
                                        aaaaCTGGTTCTATCACCTCCGTACAGGCAGTATACGTACCTGCGGATGACTTGACTGACCCGTCTCCgg                                >  1:2002623/1‑70 (MQ=255)
                                        aaaaCTGGTTCTATCACCTCCGTACAGGCAGTATACGTACCTGCGGATGACTTGACTGACCCGTCTCCGGc                               <  1:493031/71‑1 (MQ=255)
                                          aaCTGGTTCTATCACCTCCGTACAGGCAGTATACGTACCTGCGGAtgacttga                                               >  1:56397/1‑53 (MQ=255)
                                            ccggTTCGATCACCTCGATCCAGGCCGTCTACTTCCCTGCGGATGACTTGACCGACCCGTCGCCTGCAacc                           <  1:308413/69‑1 (MQ=255)
                                            ccggTTCGATCACCTCGATCCAGGCCGTCTACGTCCCTGCGGATGACTTGACCGACCCGTCGCCTGCAacc                           <  1:2660082/69‑1 (MQ=255)
                                                ttCTATCACCTCCGTACAGGCAGTATACGTACCTGCGGATGACTTGACTGACCCGTCTCCGGCAACCACCt                       <  1:2206227/71‑1 (MQ=255)
                                                ttCTATCACCTCCGTACAGGCAGTATACGTACCTGCGGATGACTTGACTGACCCGTCTCCGGCAACCACCt                       <  1:1712074/71‑1 (MQ=255)
                                                 tCTATCACCTCCGTACAGGCAGTATACGTACCTGCGGATGACTTGACTGACCCGTCTCCGGCAACCACCtt                      >  1:766038/1‑71 (MQ=255)
                                                     tCACCTCCGTACAGGCAGTATACGTACCTGCGGATGACTTGACTGACCCGTCTCCGGCAACCACCTTTgcg                  <  1:2727892/71‑1 (MQ=255)
                                                     tCACCTCCGTACAGGCAGTATACGTACCTGCGGATGACTTGACTGACCCGTCTCCGGCAACCACCTTTgc                   <  1:1765683/70‑1 (MQ=255)
                                                     tCACCTCCGTACAGGCAGTATACGTACCTGCGGATGACTTGACTGACCCGTCTCCGGCAACCACCTTTgc                   <  1:2547745/70‑1 (MQ=255)
                                                       aCCTCCGTACAGGCAGTATACGTACCTGCGGATGACTTGACTGACCCGTCTCCGGCAACCACCTTTGCGCa                >  1:3277779/1‑71 (MQ=255)
                                                            cGTACAGGCAGTATACGTACCTGCGGATGACTTGACTGACCCGTCTCCGGCAACCACCTTTGCGCACCTTg           <  1:58466/71‑1 (MQ=255)
                                                            cGTACAGGCAGTATACGTACCTGCGGATGACTTGACTGACCCGTCTCCGGCAACCACCTTTGCGCACCTTg           <  1:836866/71‑1 (MQ=255)
                                                            cGTACAGGCAGTATACGTACCTGCGGATGACTTGACTGACCCGTCTCCGGCAACCACCTTTGCGCACCTTg           <  1:2235342/71‑1 (MQ=255)
                                                            cGTACAGGCAGTATACGTACCTGCGGATGACTTGACTGACCCGTCTCCGGCAACCACCTTTGCGCACCTTg           <  1:2170098/71‑1 (MQ=255)
                                                                cAGGCAGTATACGTACCTGCGGATGACTTGACTGACCCGTCTCCGGCAACCACCTTTGCGCACCTTGACGc       <  1:816009/71‑1 (MQ=255)
                                                                cAGGCAGTATACGTACCTGCGGATGACTTGACTGACCCGTCTCCGGCAACCACCTTTGCGCACCTTGACGc       <  1:1677862/71‑1 (MQ=255)
                                                                     aGTATACGTACCTGCGGATGACTTGACTGACCCGTCTCCGGCAACCACCTTTGCGCACCTTGACGCAACCg  <  1:1780917/71‑1 (MQ=255)
                                                                     aGTATACGTACCTGCGGATGACTTGACTGACCCGTCTCCGGCAACCACCTTTGCGCACCTTGACGCAACCg  <  1:1448072/71‑1 (MQ=255)
                                                                     |                                                                      
GGAAGAGATGGGCGTTCTGCAGGAACGTATCACCTCCACCAAAACTGGTTCTATCACCTCCGTACAGGCAGTATACGTACCTGCGGATGACTTGACTGACCCGTCTCCGGCAACCACCTTTGCGCACCTTGACGCAACCG  >  Exported/2469837‑2469976

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: