Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	Exported	348,496	A→C	5.2%	N265T (AAC→ACC)	gcl →	glyoxylate carboligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	Exported	348,496	0	A	C	5.2%	227.4 / 6.1	77	N265T (AAC→ACC)	gcl	glyoxylate carboligase
Reads supporting (aligned to +/- strand):  ref base A (37/36);  new base C (2/2);  total (39/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.13e-01

TATCCCGGACGATCATGAACTGATGGCCGGGATGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGGTGTTTGGTATCGGTAACCGTTTTGCTAACCGTCATACC  >  Exported/348431‑348560                                                                  |                                                                 tATCCCGGACGATCATGAACTTATGGCCGGGATGGTGGGTCTGCAAACCGCGCATCGTTACGGTaacgc                                                               <  1:234385/69‑1 (MQ=255) tATCCCGGACGATCATGAACTGATGGCCGGGATGGTGGGTCTGCAAACCGCGCATCGTTACGGTaacgc                                                               <  1:2042594/69‑1 (MQ=255)   tCCCGGACGATCATGAACTGATGGCCGGGATGGTGGGTCTGCAAACCGCGCATCGTTACGGTaacgcaacg                                                           >  1:695045/1‑71 (MQ=255)   tCCCGGACGATCATGAACTGATGGCCGGGATGGTGGGTCTGCAAACCGCGCATCGTTACGGTaacgcaacg                                                           >  1:3182296/1‑71 (MQ=255)   tCCCGGACGATCATGAACTGATGGCCGGGATGGTGGGTCTGCAAACCGCGCATCGTTACGGTaa                                                                  >  1:2843025/1‑64 (MQ=255)     ccGGACGATCATGAACTGATGGCCGGGATGGTGGGTCTGCAAACCGCGCATCGTTACGGTaac                                                                 >  1:1786996/1‑63 (MQ=255)     ccGGACGATCATGAACTGATGGCCGGGATGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACgct                                                         >  1:2251056/1‑71 (MQ=255)     ccGGACGATCATGAACTGATGGCCGGGATGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACgct                                                         >  1:1035695/1‑71 (MQ=255)     ccGGACGATCATGAACTGATGGCCGGGATGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACgct                                                         >  1:1169986/1‑71 (MQ=255)     ccGGACGATCATGAACTGATGGCCGGGATGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACgct                                                         >  1:1816790/1‑71 (MQ=255)     ccGGACGATCATGAACTGATGGCCGGGATGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACgct                                                         >  1:1601472/1‑71 (MQ=255)       ggACGATCATGAACTGATGGCCGGGATGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACgctgc                                                       >  1:3104841/1‑71 (MQ=255)        gACGATCATGAACTGATGGCCGGGATGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACgctgct                                                      <  1:2165573/71‑1 (MQ=255)        gACGATCATGAACTGATGGCCGGGATGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACgctgct                                                      <  1:2311478/71‑1 (MQ=255)        gACGATCATGAACTGATGGCCGGGATGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACgctgct                                                      <  1:3326137/71‑1 (MQ=255)        gACGATCATGAACTGATGGCCGGGATGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACgctgct                                                      >  1:147836/1‑71 (MQ=255)           gATCATGAACTGATGGCCGGGATGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACgctgct                                                      <  1:2203558/68‑1 (MQ=255)              cATGAACTGATGGCCGGGATGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTc                                                >  1:776064/1‑71 (MQ=255)              cATGAACTGATGGCCGGGATGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTc                                                >  1:936106/1‑71 (MQ=255)                tGAACTGATGGCCGGGATGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTg                                              <  1:1104799/71‑1 (MQ=255)                tGAACTGATGGCCGGGATGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTg                                              <  1:98013/71‑1 (MQ=255)                 gAACTGATGGCCGGGATGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGa                                             >  1:3245549/1‑71 (MQ=255)                 gAACTGATGGCCGGGATGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGa                                             >  1:2729795/1‑71 (MQ=255)                 gAACTGATGGCCGGGATGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGa                                             >  1:1801254/1‑71 (MQ=255)                 gAACTGATGGCCGGGATGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGa                                             >  1:1883506/1‑71 (MQ=255)                 gAACTGATGGCCGGGATGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGa                                             >  1:1469423/1‑71 (MQ=255)                 gAACTGATGGCCGGGATGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGa                                             >  1:3173954/1‑71 (MQ=255)                 gAACTGATGGCCGGGATGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGa                                             >  1:1202364/1‑71 (MQ=255)                  aaCTGATGGCCGGGATGGTGGGTCTGCAAACCGCGCATCGTTACGGTaacgc                                                               <  1:3172380/52‑1 (MQ=255)                  aaCTGATGGCCGGGATGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACgct                                                         <  1:45620/58‑1 (MQ=255)                  aaCTGATGGCCGGGATGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGa                                             <  1:2773190/70‑1 (MQ=255)                          gCCGGGATGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGgtgt                                     <  1:2135835/70‑1 (MQ=255)                              ggATGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGGTGTTTgg                                 >  1:1355859/1‑70 (MQ=255)                                aTGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGGTGTTTGGTAt                              <  1:1297335/71‑1 (MQ=255)                                aTGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGGTGTTTGGTAt                              >  1:1481189/1‑71 (MQ=255)                                  ggtggGTCTGCAAACCGCGCATCGTTACGGTAACGCAACgctgc                                                       <  1:3339410/44‑1 (MQ=255)                                   gtggGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGGTGTTTGGTATCg                            <  1:2264146/70‑1 (MQ=255)                                    tggGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGGTGTTTGGTATCgg                           <  1:2943657/70‑1 (MQ=255)                                    tggGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGGTGTTTGGTATCgg                           <  1:2509070/70‑1 (MQ=255)                                         cTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGGTGTTTGGTATCGGTAAcc                      >  1:612440/1‑70 (MQ=255)                                         cTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGGTGTTTGGTATCGGTAACCg                     >  1:2190320/1‑71 (MQ=255)                                         cTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGGTGTTTGGTATCGGTAACCg                     >  1:2636986/1‑71 (MQ=255)                                          tGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGGTGttt                                   <  1:2441978/56‑1 (MQ=255)                                          tGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGGTGttt                                   <  1:3455673/56‑1 (MQ=255)                                          tGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGGTGttt                                   <  1:1927216/56‑1 (MQ=255)                                          tGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGGTGttt                                   <  1:724459/56‑1 (MQ=255)                                            cAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGGTGTTTGGTATCGGTAACCGttt                  <  1:3142899/71‑1 (MQ=255)                                            cAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGGTGTTTGGTATCGGTAACCGttt                  >  1:2059699/1‑71 (MQ=255)                                            cAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGGTGTTTGGTATCGGTAACCGttt                  <  1:2459885/71‑1 (MQ=255)                                             aaaCCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGGTGTTTGGTATCGGTAACCGttt                  <  1:2006405/70‑1 (MQ=255)                                              aaCCGCGCATCGTTACGGTAACGCAACGCTGCTGGCTTCTGACATGGTGTTTGGt                                <  1:2855934/55‑1 (MQ=255)                                              aaCCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGGTGTTTGGt                                <  1:3232526/55‑1 (MQ=255)                                              aaCCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGGTGTTTGGt                                <  1:2460028/55‑1 (MQ=255)                                              aaCCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGGTGTTTGGTATCGGTAACCGTTTTg                <  1:2803975/71‑1 (MQ=255)                                               aCCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGGTGTTTGGTATCGGTAACCGTTTTGc               >  1:809175/1‑71 (MQ=255)                                                   agcAGCGTTGCGTTACCGTAACGATGCTGGCGTCTGACATGGTGTTTGGTATCGGTAACCGTTTTGCTAAc           >  1:1068052/2‑71 (MQ=21)                                                   agcAGCGTTGCGTTACCGTAACGATGCTGGCGTCTGACATGGTGTTTGGTATCGGTAACCGTTTTGCTAAc           >  1:501409/2‑71 (MQ=21)                                                    gcATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGGTGTTTGGTATCGGTAACCGTTTTGCTAAcc          >  1:3214182/1‑71 (MQ=255)                                                    gcATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGGTGTTTGGTATCGGTAACCGTTTTGCTAAcc          >  1:691588/1‑71 (MQ=255)                                                    gcATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGGTGTTTGGTATCGGTAACCGTTTTGCTAAcc          >  1:1003651/1‑71 (MQ=255)                                                    gcATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGGTGTTTGGTATCGGTAACCGTTTTGCTAAcc          >  1:1688327/1‑71 (MQ=255)                                                    gcATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGGTGTTTGGTATCGGTAACCGTTTTGCTAAcc          >  1:1901217/1‑71 (MQ=255)                                                    gcATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGGTGTTTGGTATCGGTAACCGTTTTGCTAAcc          >  1:2096109/1‑71 (MQ=255)                                                      agcGTTGCGTTACCGTAACGATGCTGGCGTCTGACATGGTGTTTGGTATCGGTAACCGTTTTGCTAACCGt        <  1:2760212/69‑1 (MQ=25)                                                      agcGTTGCGTTACCGTAACGATGCTGGCGTCTGACATGGTGTTTGGTATCGGTAACCGTTTTGCTAACCGt        <  1:1000793/69‑1 (MQ=25)                                                      aTCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGGTGTTTGGTATCGGTAACCGTTTTGCTAACCGt        <  1:3257243/71‑1 (MQ=255)                                                      aTCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGGTGTTTGGTATCGGTAACCGTTTTGCTAACCGt        <  1:3056253/71‑1 (MQ=255)                                                      aTCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGGTGTTTGGTATCGGTAACCGTTTTGCTAACCGt        <  1:774620/71‑1 (MQ=255)                                                      aTCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGGTGTTTGGTATCGGTAACCGTTTTGCTAACCGt        <  1:2749386/71‑1 (MQ=255)                                                      aTCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGGTGTTTGGTATCGGTAACCGTTTTGCTAACCGt        <  1:2189034/71‑1 (MQ=255)                                                        cGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGGTGTTTTGTATCGGTAACCg                     >  1:2719987/1‑56 (MQ=255)                                                        cGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGGTGTTTGGTATCGGTAACCGTTTTGCTAACCGTCa      >  1:557335/1‑71 (MQ=255)                                                        cGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGGTGTTTGGTATCGGTAACCGTTTTGCTAACCGTCa      >  1:2700980/1‑71 (MQ=255)                                                        cGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGGTGTTTGGTATCGGTAACCGTTTTGCTAACCGTCa      >  1:2705017/1‑71 (MQ=255)                                                         gTTACGGTAACGCAACGCTGCTGGCGTCTGACATGGTGTTTGGTATCGGTAACCGTTTTGCTAACCGTCa      >  1:3198605/1‑70 (MQ=255)                                                          ttACGGTAACGCAACGCTGCTGGCGTCTGACATGGTGTTTGGTATCgg                           <  1:585307/48‑1 (MQ=255)                                                          ttACGGTAACGCAACGCTGCTGGCGTCTGACATGGTGTTTGGTATCGGTAACCGTTTTGCTAACCGTCATa    <  1:1628150/71‑1 (MQ=255)                                                          ttACGGTAACGCAACGCTGCTGGCGTCTGACATGGTGTTTGGTATCGGTAACCGTTTTGCTAACCGTCATa    <  1:872001/71‑1 (MQ=255)                                                          ttACGGTAACGCAACGCTGCTGGCGTCTGACATGGTGTTTGGTATCGGTAACCGTTTTGCTAACCGTCATa    <  1:1204832/71‑1 (MQ=255)                                                            aCGGTAACGCAACGCTGCTGGCGTCTGACATGGTGTTTGGTATCGGTAACCGTTTTGCTAACCGTCATAcc  >  1:2532854/1‑71 (MQ=255)                                                            aCGGTAACGCAACGCTGCTGGCGTCTGACATGGTGTTTGGTATCGGTAACCGTTTTGCTAACCGTCATAcc  >  1:2184626/1‑71 (MQ=255)                                                            aCGGTAACGCAACGCTGCTGGCGTCTGACATGGTGTTTGGTATCGGTAACCGTTTTGCTAACCGTCATAcc  >  1:2010621/1‑71 (MQ=255)                                                             cGGTAACGCAACTCTGCTGGCGTCTGACATGGTGTTTGGTATCGGTAACCGTTTTGCTAACCGTCAt     <  1:1498821/67‑1 (MQ=255)                                                              ggTAACGCAACGCTGCTGGCGTCTGACATGGTGTTTGGTATCGGTAACCGTTTTGCTAACCGTCAt     <  1:2671314/66‑1 (MQ=255)                                                                  |                                                                 TATCCCGGACGATCATGAACTGATGGCCGGGATGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGGTGTTTGGTATCGGTAACCGTTTTGCTAACCGTCATACC  >  Exported/348431‑348560

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑