| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | BS168_reference_genome | 4,090,006 | T→G | 12.9% | Y435S (TAC→TCC) | htpG ← | class III heat‑shock protein (ATP‑dependent molecular chaperone HSP90) |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | BS168_reference_genome | 4,090,006 | 0 | T | G | 12.9% | 131.9 / 4.7 | 62 | Y435S (TAC→TCC) | htpG | class III heat‑shock protein (ATP‑dependent molecular chaperone HSP90) |
| Reads supporting (aligned to +/- strand): ref base T (17/36); new base G (7/2); total (24/38) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.15e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TTTCCTGATAAGAAGCCAGCATTTTAATTGCAAACTCATCGATGTCCTCAGTAAAGTAGAGGATTTCATAGCCTTTGTCCGCCACCATTTCCGTTTGCGGCAGTTTCTCAATGCGGTCGTAAGAATCACCGGTCGCGTAATAAATGAATTTCTGCTCCTCTGGCATTCTGGAAACATACTCGTCTAAGCTGACCAGTTTTTTCTCTTTTGAAGAGTAGAATAAGAGGAGATCCTTCAATTGATCCTTATTCGCGCCAAAATCATTGTACACGCCG > BS168_reference_genome/4089869‑4090143 | tttCCTGATAAGAAGCCAGCATTTTAATTGCAAACTCATCGATGTCCTCAGTAAAGTAGAGGATTTCATAGCCTTTGTCCGCCACCATTTCCGTTTGCGGCAGTTTCTCAATGCGGTCGTAAGAATCACCGGTCGAGt > 5:201101/1‑138 (MQ=255) agaagCCAGCATTTTAATTGCAAACTCATCGATGTCCTCAGTAAAGTAGAGGATTTCATAGCCTTTGTCCGCCACCATTTCCGTTTGCGGCAGTTTCTCAATGCGGTCGTAAGAATCACCGGTCGCGTAATAAATGAAt < 8:51822/139‑1 (MQ=255) gCCAGCATTTTAATTGCAAACTCATCGATGTCCTCAGTAAAGTAGAGGATTTCATAGCCTTTGTCCGCCACCATTTCCGTTTGCGGCAGTTTCTCAATGCGGTCGTAAGAATCACCGGTCGCGTAATAAATTAAtttat > 7:307501/1‑137 (MQ=255) gCATTTTAATTGCAAACTCATCTATGTCCTCAGTAAAGTAGAGGATTTCATAGCCTTTGTCCGCCACCATTTCCGTTTGCGGCGGTTTCTCAATGCGGTCGTAAGAATCACCGGTCGCGTAATAAATTAATTTGTGctg > 1:46464/1‑138 (MQ=255) gCAAACTCATCGATGTCCTCAGTAAAGTAGAGGATTTCATAGCCTTTGTCCGCCACCATTTCCGTTTGCGGCAGTTTCTCAATGCGGTCGTAAGAATCACCGGTCGCGGAATAAATGGATTTATGCGGCTCGGGCATTc > 8:201776/1‑139 (MQ=255) cAAACTCATCGATGTCCTCAGTAAAGTAGAGGATTTCATAGCCTTTGTCCGCCACCATTTCCGTTTGCGGCAGTTTCTCAATGCGGTCGTAAGAATCACCGGTCGCGTAATAAATGAATTTCTGCTCCTCTGGCATTc < 2:130044/138‑1 (MQ=255) aaaCTCATCGATGTCCTCAGTAAAGTAGAGGATTTCATAGCCTTTGTCCGCCACCATTTCCGTTTGCGGCAGTTTCTCAATGCGGTCGTAAGAATCACCGGTCGCGTAATAAATGAATTTCTGCTCCTCTGGCATTCTg < 6:352025/139‑1 (MQ=255) cTCATCGATGTCCTCAGTAAAGTAGAGGATTTCATAGCCTTTGTCCGCCACCATTTCCGTTTGCGGCAGTTTCTCAATGCGGTCGTAAGAATCACCGGTCGCGTAATAAATGAATTTCTGCTCCTCTGGCATTCTGGaa < 3:219155/139‑1 (MQ=255) gATGTCCTCAGTAAAGTAGAGGATTTCCTAGCCTTTGTCCGCCACCATTTCCGTTTGCGGCAGTTTCTCAATGCGGTCGTAAGAATCACCGGTCGCGTAATAAATGAATTTCTGCTCCTCTGGCATTCAGGAAACATa < 1:329996/138‑1 (MQ=255) gATGTCCTCAGTAAAGTAGAGGATTTCATAGCCTTTGTCCGCCACCATTTCCGTTTGCGGCAGTTTCTCAATGCGGTCGTAAGAATCACCGGTCGCGTAATAAATGAATTTCTGCTCCTCTGGCATTCTGGAAACATa < 4:107042/138‑1 (MQ=255) gATGTCCTCAGTAAAGTAGAGGATTTCATAGCCTTTGTCCGCCACCATTTCCGTTTGCGGCAGTTTCTCAATGCGGTCGTAAGAATCACCGGTCGCGTAATAAATGAATTTCTGCTCCTCTGGCATTCTGGAAACATa < 5:375412/138‑1 (MQ=255) ccTCAGTAAAGTAGAGGATTTCATAGCCTTTGTCCGCCACCATTTCCGTTTGCGGCAGTTTCTCAATGCGGTCGTAAGAATCACCGGTCGCGTAATAAATGAATTTCTGCTCCTCTGGCATTCTGGAAACATACTCGTc > 7:120110/1‑139 (MQ=255) aGTAAAGTAGAGGATTTCATAGCCTTTGTCCGCCACCATTTCCGTTTGCGGCAGTTTCTCAATGCGGTCGTAAGAATCACCGGTCGCGTAATAAATGAATTTCTGCTCCTCTGGCATTCTGGAAACATa > 6:191506/1‑129 (MQ=255) aGTAAAGTAGAGGATTTCATAGCCTTTGTCCGCCACCATTTCCGTTTGCGGCAGTTTCTCAATGCGGTCGTAAGAATCACCGGTCGCGTAATAAATGAATTTCTGCTCCTCTGGCATTCTGGAAACATa < 5:191506/129‑1 (MQ=255) aGTAAAGTAGAGGATTTCATAGCCTTTGTCCGCCACCATTTCCGTTTGCGGCAGTTTCTCAATGCGGTCGTAAGAATCACCGGTCGCGTAATAAATGAATTTATGCTCCTCTGGCATTCTGGAAACATACTCGGCTAAg > 5:84325/1‑139 (MQ=255) tAAAGTAGAGGATTTCATAGCCTTTGTCCGCCACCATTTCCGTTTGCGGCAGTTTCTCAATGCGGTCGTAAGAATCACCGGTCGCGTAATAAATGAATTTCTGCTCCTCGTGCCTTAGGGAAACATACGAGTCTAAGcg > 8:21392/1‑138 (MQ=255) tAGAGGATTTCATAGCCTTTGTCCGCCACCATTTCCGTTTGCGGCAGTTTCTCAATGCGGTCGTAAGAATCACCGGTCGCGTAATAAATGCATTTCTGCTCCTCGGGCATTCTGGAAACATACTCGTCTAAGCTggc > 1:248612/1‑135 (MQ=255) tAGAGGATTTCATAGCCTTTGTCCGCCACCATTTCCGTTTGCGGCAGTTTCTCAATGCGGTCGTAAGAATCACCGGTCGCGGTATAAATTGATTTGTGCGGCTCGGGCAGTGTGGGAACAGACACGGCGAAGcgtgccc > 1:333784/1‑133 (MQ=255) tAGAGGATTTCATAGCCTTTGTCCGCCACCATTTCCGTTTGCGGCAGTTTCTCAATGCGGTCGTAAGAATCACCGGTCGCGGTATAAATTAATTTGTGCTCCGCTGGCTATCTTGAAACATACTCGTGGAAGCTGGCCa > 4:59518/1‑139 (MQ=255) tAGAGGATTTCATAGCCTTTGTCCGCCACCATTTCCGTTTGCGGCAGTTTCTCAATGCGGTCGTAAGAATCACCGGTCGCGGAATAAATGAATTTGTGCTCCTCTGGCATTCTGGAAACATACTCGGCTAAGcggtc > 4:148651/1‑133 (MQ=255) gagGATTTCATAGCCTTTGTCCGCCACCATTTCCGTTTGCGGCAGTTTCTCAATGCGGTCGTAAGAATCACCGGTCGCGTAATAAATGAATTTCTGCTCCTCTGGCATTCTGGAAACATACTCGTCTAAGCTGACCAGt < 5:396713/139‑1 (MQ=255) agGATTTCATAGCCTTTGTCCGCCACCATTTCCGTTTGCGGCAGTTTCTCAATGCGGTCGTAAGAATCACCGGTCGCGTTATAAATGAATTTGTGGTGCTCTGGGCTGAGGGAACCATACTCGGCTTAGGTGAcccggt > 1:306397/1‑135 (MQ=255) gATTTCATAGCCTTTGTCCGCCACCATTTCCGTTTGCGGCAGTTTCTCAATGCGGTCGTAAGAATCCCCGGTCGCGTTATAAATGAATTTCATGGACTAAGGCATTATTGCAACATAATCGTCGAATCTTACCaagttt > 6:101436/1‑134 (MQ=255) gATTTCATAGCCTTTGTCCGCCACCATTTCCGTTTGCGGCAGTTTCTCAATGCGGTCGTAAGAATCACCGGTCGCGGTATAAATTAATTTATGCTCCTCTGGCATTCTGGATACATACTCGTGGAAGCGGACCAGtttt > 8:271767/1‑139 (MQ=255) tttCATAGCCTTTGTCCGCCACCATTTCCGTTTGCTGCTGTTTCTCAATGCGGTCGTAAGAATCACCGGTCGCGTAATAAATGAATTTCTGCTCCTCTGGCATTCTGGAAACATACTCGTCTAAGCTGACCAGtttttt < 7:281932/139‑1 (MQ=255) tttCATAGCCTTTGTCCGCCACCATTTCCGTTTGCGGCAGTTTCTCAATGCGGGCGTAAGAATCACCGGTCGCGTAATAAATGAATTTCTGCTCCTCTGGCATTCTGGAAACATACTCGTCTAAGCTGACCAGtttttt < 7:201776/139‑1 (MQ=255) ttCATAGCCTTTGTCCGCCACCATTTCCGTTTGCGGCAGTTTCTCAATGCGGTCGTAAGAATCACCGGTCGCGTAATAAATTAATTTATTCTGCGATGGGATTCTGGAAACATACACGGCTAAGCTGCGCAGgttttta > 6:396713/1‑138 (MQ=255) ccgttGTCCGCCTCCATTTCCGTTTGCGGCAGTTTCTCAATGCGGTCGTAAGAATCACCGGTCGCGTAATAAATGAATTTCTGCTCCTCTGGCATTCTGGAAACATACTCGTCTAAGCTGACCAGTTTTTTCTCTTTTg < 6:348877/136‑1 (MQ=255) ccTTTGTCCGCCACCATTTGCGTTTGCGGCAGTTTCTCAATGCGGTCGTAAGAATCACCGGTCGCGTAATAAATGAATTTCTGCTCCTCTGGCATTCTGGAAACATACTCGTCTAAGCTGACCAGTTTTTTCTCTTTTg < 1:189020/139‑1 (MQ=255) ccTTTGTCCGCCACCATTTCCGTTTGCGGCAGTTTCTCAATGCGGTCGTAAGAATCACCGGTCGCGTAATAAATGAATTTCTGCTCCTCTGGCATTCTGGAAACATACTCGTCTAAGCTGACCAGTTTTTTCTCTTTTg < 5:203266/139‑1 (MQ=255) ccTTTGTCCGCCACCATTTCCGTTTGCGGCAGTTTCTCAATGCGGTCGTAAGAATCACCGGTCGCGTAATAAATGAATTTCTGCTCCTAAGGCATTCTGGCAAAATACTCGTATAACCTGACCAGTTTTTTGTATTTTg > 7:62253/1‑139 (MQ=255) ccTTTGTCCGCCACCATTTCCGTTTGCGGCAGTTTCTCAATGCGCTCGTAAGAATCACCGGTCGCGTAATAAATGAATTTCTGCTCCTATGGCATTGATGAAAAATTCTAGGCTAAACGGACAAGTTTTTTGTCttttt > 7:68545/1‑138 (MQ=255) ttGTCCGCCACCATTTCCGTTTGCGGCAGTTTCTCAATGCGGTCGTAAGAATCACCGGTCGCGTAATAAATGAATTTATGGTCCTCTGGCATTCTGGAAAAATAAACGTCTAAGCTGACCAGTGTTTTCTCTTTTgta > 4:96804/1‑136 (MQ=255) tgggcGCCACCCTTTCCGTTTGCGGCAGTTTCTCAATGCGGTCGTAAGAATCACCGGTCGCGTAATAAATGAATTTCTGCTCCTCTGGCATTCTGGAAACATACTCGTCTAAGCTGACCAGTTTTTTCTCTTTTGaa < 4:363647/133‑1 (MQ=255) tGTCCGCCACCATTTCCGTTTGCGGCAGTTTCTCAATGCGGTCGTAAGAATCACCGGTCGCGTAATAAATg > 2:86985/1‑71 (MQ=255) tGTCCGCCACCATTTCCGTTTGCGGCAGTTTCTCAATGCGGTCGTAAGAATCACCGGTCGCGTAATAAATg < 1:86985/71‑1 (MQ=255) tGTCCGCCACCATTTCCGTTTGCGGCAGTTTCTCAATGCGGTCGTAAGAATCACCGGTCGCGGAATAAAATAATTTATTGTCCGAAGGCAATATGGAAAAATAAACGTATAAAATGACCACTTTTTTATGTTTTGaata > 6:203266/1‑137 (MQ=255) tGTCCGCCACCATTTCCGTTTGCGGCAGTTTCTCAATGCGGTCGTAAGAATCACCGGTCGAGTAATAAATGAATTTCTGCTCCTCTGGCATTCTGGAAACATACTCGTCTAAGCTGACCAGTTTTTTCTCTTTTGAag < 1:370238/138‑1 (MQ=255) tCCGCCACCATTTCCGTTTGCGGCAGTTTCTCAATGCGGTCGTAAGAATCACCGGTCGCGTAATAAATGAATTTCTGCTCCTCTGGCATTCTGGAAACATACTCGTCTAAGCTGACCAGTTTTTTCTCTTTTGAagag < 3:96804/138‑1 (MQ=255) tCCGCCACCATTTCCGTTTGCGGCAGTTTCTCAATGCGGTCGTAAGAATCACCGGTCGCGTAATAAATGAATTTCTGCTCCTCTGGCATTCTGGAAACATACTCGTCTAAGCTGACCAGTTTTTTCTCTTTTGAagag < 2:213269/138‑1 (MQ=255) tCCGCCACCATTTCCGTTTGCGGCAGTTTCTCAATGCGGTCGTAAGAATCACCGGTCGCGTAATAAATGAATTTCTGCTCCTCTGGCATTCTGGAAACATACTCGTCTAAGCTGACCAGTTTTTTCTCTTTTGAagag < 4:258221/138‑1 (MQ=255) tCCGCCACCATTTCCGTTTGCGGCAGTTTCTCAATGCGGTCGTAAGAATCACCGGTCGCGTAATAAATGAATTTCTGCTCCTCTGGCATTCTGGAAACATACTCGTCTAAGCTGACCAGTTTTTTCTCTTTTGAagag < 7:372745/138‑1 (MQ=255) tCCGCCACCATTTCCGTTTGCGGCAGTTTCTCAATGCGGTCGTAAGAATCACCGGTCGCGTAATAAATGAATTTCTGCTCCTCTGGCATTCTGGAAACATACTCGTCTAAGCTGACCAGTTTTTTCTCTTTTGAagag < 5:230582/138‑1 (MQ=255) tCCGCCACCATTTCCGTTTGCGGCAGTTTCTCAATGCGGTCGTAAGAATCACCGGTCGCGTAATAAATGAATTTCTGCTCCTCTGGCATTCTGGAAACATACTCGTCTAAGCTGACCAGTTTTTTCTCTTTTGAAGAGt < 8:120110/139‑1 (MQ=255) tCCGCCACCATTTCCGTTTGCGGCAGTTTCTCAATGCGGTCGTAAGAATCACCGGTCGCGTAATAAATGAATTTCTGCTCCTCTGGCATTCTGGAAACATACTCGTCTAAGCTGACCAGTTTTTTCTCTTTTGAAGAGt < 3:148651/139‑1 (MQ=255) cGCCACCATTTCCGTTTGCGGCAGGTACTCAATGCGCGCGTAAGACTCACCGGTCGCGTAATAAATGTATTTCTGCTCCTCTGGCATTCTGGAAACATACTCGTCTCCGCTTACCAGTTGTTTCTCATTTGAAGAGTAg < 4:397660/139‑1 (MQ=255) cGCCACCAGTTCCGTTTGCGGCAGTTTCTCTATGCGGTCGTAAGAAGCAGCGGGCGCGGAATTAATGAAGTTCTGCTCCTCTGGCATTCTGGAAACATACTCGTCTAAGCTGACCAGTTTTTTCTCTTTTGAAGAGTAg < 8:225931/139‑1 (MQ=255) ccaccaTTTCCGTTTGCGGCAGTTTCTCAATGCGGTCGTAAGAATCACCGGTCGCGTTATAAATTAATTTATGCTACACTGGGCTTCTTGAAACAAACACGTCTAATCTGACCAGTTTTTTATATTTTTAAGAGttta > 6:316896/1‑135 (MQ=255) ccaccaTTTCCGTTTGCGGCAGTTTCTCAATGCGGTCGTAAGAATCACCGGTCGCGGAAAAAATGAATTTCTGCTCCGCGGGCATTCTGGGAACAAACTCGTCTAAGCTGACCAGGTTTTTTGGTTTTTTAGAGTAGaa > 3:362535/1‑139 (MQ=255) tttACGTTTGCGGCAGTTTCTCAATGCGGTCGTAAGAATCACCGGTCGCTTAATAAATGAATTTCTGCTCCTCTGGCATTCTGGAAACATACTCGTCTAAGCTGACCAGTTTTTTCTCTTTTGAAGAGTAGAATAagag < 6:39058/139‑1 (MQ=255) ccttttGCGGCAGTTTCTCAATGCGGTCGTAAGAATCACCGGTCGCGTAATAAATGAATTTCTGCTCCTCTGGCATTCTGGAAACATACTCGTCTAAGCTGACCAGTTTTTTCTCTTTTGAAGAGTAGAATAAgagga < 6:86460/135‑1 (MQ=255) ccGTTTGCGGCAGTTTCTCAATGCGGTCGTAAGAATCACCGGTCGCGTAATAAATGAATTTCTGCTCCTCTGGCATTCTGGAAACATACTCGTCTAAGCTGACCAGTTTTTTCTCTTTTGAAGAGTAGAATAAgagga < 5:316896/138‑1 (MQ=255) tGCGGCAGTTTCTCAATGCGGTCGTAAGAATCACCGGTCGCGTAATAAATGAATTTCTGCTCCTCTGGCATTCTGGAAACATa < 8:345114/83‑1 (MQ=255) tGCGGCAGTTTCTCAATGCGGTCGTAAGAATCACCGGTCGCGTAATAAATGAATTTATGCTCCTCTGGCATTCTGGAAACATa > 7:345114/1‑83 (MQ=255) gCGGCAGTTTCTCAATGCGGTCGTAAGAATCACCGGTCGCGGAATAAATGAATTTCTGCTCCTCTGGCATTCTGGAAACATACTCGTCTAAGCTGACCAGTTTTTTCTCTTTTGAAGAGTAGAATAAGAGGAGATCCt < 2:80497/138‑1 (MQ=255) cAGTTTCTCAATGCGGTCGTAAGAATCACCGGTCGCGTAATAAATGAATTTCTGCTCCTCTGGCATTCTGGAAACATACTCGTCTAAGCTGACCAGTTTTTTCTCTTTTGAAGAGTAGAATAAGAGGAGATCCTTCAAt < 3:59518/139‑1 (MQ=255) cGGTCGTAAGAATCACCGGTCGCGTAATAAATGAATTTCTGCTCCTCTGGCATTCTGGAAACATACTCGTCTAAGCTGAc > 5:375093/1‑80 (MQ=255) cGGTCGTAAGAATCACCGGTCGCGTAATAAATGAATTTCTGCTCCTCTGGCATTCTGGAAACATACTCGTCTAAGCTGAc < 6:375093/80‑1 (MQ=255) tAAGAATCACCGGTCGCGTAATAAATGAATTTCTGCTCCTCTGGCATTCTGGAAACATACTCGTCTAAGCTGACCAGTTTTTTCTCTTTTGAAGAGTAGAATAAGAGGAGATCCTTCAATTGATCCTTATTCGCGCCaa < 5:28450/139‑1 (MQ=255) tAAGAATCACCGGTCGCGTAATAAATGAATTTCTGCTCCTCTGGCATTCTGGAAACATACTCGTCTAAGCTGACCAGTTTTTTCTCTTTTGAAGAGTAGAATAAGAGGAGATCCTTCAATTGATCCTTATTCGCGCCaa < 5:20568/139‑1 (MQ=255) gcgTAATAAATGATTTTCAGCTCCTCTGGCATTCTGGAAACATACTCGTCAAAGCTGACCAGTTTTTTCTCTTTTGAAGAGTAGAATAAGAGGAGATCCTTCAATTGATCCTTATTCGCGCCAAAATCATTGTACACGc < 8:68545/139‑1 (MQ=255) gcgTAATAAATGAATTTCTGCTCGTCTGGCATTGTGGAAACATACTCGGCTAAGCTGACCAGATTTTTCTCTTTTGAAGAGTAGAATAAGAGGAGATCCTTCAATTGATCCTTATTCGCGCCAAAATCATTGTACACGc < 8:62253/139‑1 (MQ=255) gTAATAAATGAATTTCTGCTCCTCTGGCATTCTGGAAACATACTCGTCTAAGCTGACCAGTTTTTTCTCTTTTGAAGAGTAGAATAAGAGGAGATCCTTCAATTGATCCTTATTCGCGCCAAAATCATTGTACACGCCg < 7:271767/139‑1 (MQ=255) | TTTCCTGATAAGAAGCCAGCATTTTAATTGCAAACTCATCGATGTCCTCAGTAAAGTAGAGGATTTCATAGCCTTTGTCCGCCACCATTTCCGTTTGCGGCAGTTTCTCAATGCGGTCGTAAGAATCACCGGTCGCGTAATAAATGAATTTCTGCTCCTCTGGCATTCTGGAAACATACTCGTCTAAGCTGACCAGTTTTTTCTCTTTTGAAGAGTAGAATAAGAGGAGATCCTTCAATTGATCCTTATTCGCGCCAAAATCATTGTACACGCCG > BS168_reference_genome/4089869‑4090143 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |