| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | BS168_reference_genome | 4,093,357 | A→C | 16.7% | S161A (TCT→GCT) | yxbF ← | putative transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | BS168_reference_genome | 4,093,357 | 0 | A | C | 16.7% | 89.9 / 2.8 | 60 | S161A (TCT→GCT) | yxbF | putative transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base A (23/26); new base C (2/9); total (25/35) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.01e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCCTCTCTCGTTCGAACCACCCGCCTGTCGATTTGCTTCTCCTTCTCTCGCTCTCCTGGCACAGCTTTTCCAAACAGCCAATAAAACCGCTTTTGAGAAACAAGCTCCCATACCTTCTGGCTGATGGCTTCAGGAGACGCGGCCGCATCCTCATTCAGCCAATACAGGATAATCGCGTACGTGTACAAGGCCCGATAATGTCCATAAATGTCCCGTTCAATCGGCGTTAAATTCACATGGATCGGAGCCAGCAGCACATCCTGCTGAAACACATC > BS168_reference_genome/4093221‑4093495 | cccgctcgcGTTCCAACCCCCCGCCTGTCGATTTGCTGCTCCTTCTCTCGCTCTCCTGCCACAGCTTTTCCAAACAGCCAAAAAACCCGCTTTTGACAAACAAGCTCCCATACCTTCTGGCTGATGGCTTCAGGAGCcg < 8:310609/135‑1 (MQ=255) ctctcGTTCGAACCACCCGCCTGTCGATTTGCTTCTCCTTCTCTCGCTCTCCTGGCACAGCTTTTCCAAACAGCCAATAAAACCGCTTTTGAGAAACAAGCTCCCATACCTTCTGGCTGATGGCTTCAGGAGACGCGGc < 4:264886/139‑1 (MQ=255) cacgcGTTCGACCCCCCCGCCTGTCTATTGGCTTCTCATTCGAGCGCTCTCCTGCCACCCCTTTTCCAAACACCCAATAAACCCGCTTTTGAGAACCACGCTCCCATACCTTCTGGCTCATGGCTGACGCAGACGCGGc < 8:77220/135‑1 (MQ=255) accaccCGCCTGGCGATTTGCTTGTCATTCTCTCGCTCTCGTGTCACATCTTTACCAAACTGCCAATAAAACCGGTTTTGATAAACAAGCTCCCAAACCTTCTGGCTGATGGCTTCAGGAGCCGCGGCCGCATCCTCAt < 8:229477/139‑1 (MQ=255) acaaccCGCCTGTATATTTGCTTCTCCTTCTCTCGCTCTCCTGGAACAGATTTACCAAACACCCAATAAAACCGCTTTTGAGAAACAAGCTCCCATACATTCTGGCTGATGGCTTAAGGAGACGCGGCCGCATCCTCAt < 5:297515/136‑1 (MQ=255) ccGCCTGTCGATTTGCTTCTCCTTCTCTCGCTCTCCTGGCACAGCTTTTCCAAACAGCCAATAAAACCGCTTTTGAGAAACAAGCTCCCATACCTTCTGGCTGATGGCTTCAGGAGACGCGGCCGCATCCTCATTCAGc > 7:351984/1‑139 (MQ=255) ccGCCTGTCGATTTGCTTCTCCTTCTCTCGCTCTCCTGGCACAGCTTTTCCAAACAGCCAATAAAACCCCTTTTGAGAAACAACCTCCCATACCTTCTGGCTTATGGCTTCAGGAGACGCGGCCGCATCCTCATTCAGc < 7:80488/139‑1 (MQ=255) ccGCCTGTCGATTTGCTTCTCCTTCTCTCGCGCTCCTGGCACAGCTTTTCCAAACAGCCAATAAACCCGCTTTTGAGAAACAAGCTCCCATACCTTCTGGCTGATGGCTTCAGGAGACGCGGCCGCATCCTCATTCAGc < 1:72794/139‑1 (MQ=255) ccGCCTGTCGATTTGCTTCTCCTTCTCTCGATCTCCTGGAACAGCTTTTCCAAACAGACAATAAAACCGTTTTTGAGAAACAAGCTCCAATACCTTCTGGGTGTTGGGTCCAGGAGCCGCGGCCGCATCCTCATTCAGc < 4:160442/139‑1 (MQ=255) tCGATTTGCTTCTCCTTCTCTCGCTCTCCTGGCACAGCTTTTCCAAACAGCCAATAAAACCGCTTTTGAGAAACAAGCTCCCATACCTTCTGGCTGATGGCTTCAGGAGACGCGGCCGCATCCTCATTCAGCCAATa < 8:32634/137‑1 (MQ=255) tCGATTTGCTTCTCCTTCTCTCGCTCTCCTGGCACAGCTTTTCCAAACAGCCAAAAAAACCGTTTTTGATAAACAAGCTCCCATACCTTCTGGCTGATGGCTTCTGGAGACGCGGCCGCATCCTCATTCAGCCAATa < 6:76226/137‑1 (MQ=255) ttGCTTCTCCTTCTCTCGCTCTCCTGGCACAGCTTTTCCAAACAGCCAATAAAACCGCTTTTGAGAAACAAGCTCCCATACCTTCTGGCTGATGGCTTCAGGAGACGCGGCCGCATCCTCATTCAGCCAATACAGGAt < 8:243645/138‑1 (MQ=255) cttctcTCGCTCTCCTGGCACAGCTTTTCCAAACAGCCAATAAAACCGCTTTTGAGAAACAAGCTCCCATACCTTCTGGCTGATGGCTTCAGGAGACGCGGCCGCATCCTCATTCAGCCAATACAGGATAATCGCGTAc > 2:348351/1‑139 (MQ=255) cttctcTCGCTCTCCTGGCACAGCTTTTCCAAACAGCCAATAAAACCGCTTTTGAGAAACAAGCGCCCATACCTTCTGGCTGATGGCTTCAGGAGACGCGGCCGCATCCTCATTCAGCCAATACAGGATAATCGCGTAc < 2:74488/139‑1 (MQ=255) attctcTCGCTCACCTGGCCCAGCTTTACCTACCCGCAAATAAAACCGCTTTTGAGAACCAACCTCCCATCCCTACTGGCTGTTGGCTTCAGGAGACGCGGCCGCATCCTCATTCAGCCAATACAGGATAATCGCGTAc < 6:117499/138‑1 (MQ=255) ctcGCTCTCCTGGCACAGCTTTTCCAAACAGACAAAAAAACCGCTTTTGAGAAACAAGCTCCCATACCTTCTGGCTGATGGCTTCTGGAGACGCGGCCGCATCCTCATTCAGCCAATACAGGATAATCGCGTACGTGTa < 6:81996/139‑1 (MQ=255) ggCTCTCCTGGAACATCTTTTCCAAACACCCAATAAAACCGCTTTTGAGAAACAAGCTCCCATACCTTCTGGCTGTGGGCTTCAGGTGACGCGGCCGCATCCTCATTCAGCCAATACAGGATAATCGCGTACGTGTACa < 3:398317/138‑1 (MQ=255) cGCTCTCCTGGAAACGATTTTCAACACCCCCAATAAAACCGATTTTGAGAAACAAGCGCCCATCCCTTCTGGCTTATGTCTTCAGGATACGCGGCCGCATCCTCATTCAGCCAATACAGGATAATCGCGTACGTGTACa < 4:47970/139‑1 (MQ=255) gCTCTCCTGGCACAGCTTTTCCAAACAGCCAATAAAACCGCTTTTGAGAAACAAGCTCCCATACCTTCTGGCTGATGGCTTCAGGAGACGCGGCCGCATCCTCATTCAGCCAATACAGGATAATCGCGTACGTGTACa < 7:275220/138‑1 (MQ=255) gcccaGCTTTTCCAACCTGCCAATAAAACGGCTTTTGAGACAGACGCTCCCATACGTGCTGGCGGCGGGCTGCAGGAGACGCGGCCGCATCCTCATTCAGCCAATACAGGATAATCGCGTACGTGTACAAGGCCCGATa < 8:73836/136‑1 (MQ=255) gaacaGCTTTTCCAAACACCCAATAAAACCGCTGTTGAGAAACAAGCTCCCATACCTTCTGGCTGATGGCTTCAGGAGCCGCGGCCGCATCCTCATTCAGCCAATACAGGATAATCGCGTACGTGTACAAGGCCCGATa < 8:16934/137‑1 (MQ=255) gCACACCTTAGCAAAACACCCAAAAAACCCGCTTTCGAGAAACACGCTCCCAAACCTTCTGGCTGAGGGCTTCAGGCCCCGCGGCCGCATCCTCATTCAGCCAATACAGGATAATCGCGTACGTGTACAAGGCCCGATa < 8:211428/139‑1 (MQ=255) gCTTTTCCAAACAGCCAATAAAACCGCTTTTGAGAAACAAGCTCCCATACCTTCTGGCTGATGGCTTCAGCAGCCGCGGCCGCATCCACAATCCCCCAATACCGGATAATAGCGAACCTTTTCAAGCCCCCATAATGt > 2:186289/1‑138 (MQ=255) ttttCCAAACAGCCAATAAAACCGCTTTTGAGAAACAAGCTCCCATCCCTTCTGGCTGATGGCTTCAGGAGACGCGGCCGCATCCTCATTCAGCCAATACAGGATAATCGCGTACGTGTACAAGGCCCGATAATGTc < 1:348351/137‑1 (MQ=255) ccAAACAGCCAATAAAACCGCTTTTGAGAAACAAGCTCCCATACCTTCTGGCTGATGGCTTCAGGAGACGCGGCCGCATCCTCATTCAGCCAATACAGGATAATCGCGTACGTGTACAAGGCCCGATAATGTCCATaaa > 3:337756/1‑139 (MQ=255) ccAAACAGCCAAAAAACCCGCTTTTGAGAAACACGCACCCATACCTTCGGGCTGAGGGCTGCCGGAGACGCGGCCGCATCCTCATTCAGCCAATACAGGATAATCGCGTACGTGTACAAGGCCCGATAATGTCCATaa < 6:396324/138‑1 (MQ=255) caaaaCAGCAAAAAAACCCCCTTTTGACAACCACGCCCCACTACCTTCGGGCTGCTGCCGTCAGGATCCGCGGCCGCATCCTCATTCAGCCAATACAGGATAATCGCGTACGTGTACAAGGCCCGATAATGTCCATaa < 4:38497/136‑1 (MQ=255) aaaCACCAAATAAAACCGCTTTTGTCAAACATCCTCCCATACCTTCGGGCTTAGGGCTTCCGGACCCGCGGCCGCATCCTCATTCAGCCAATACAGGATAATCGCGTACGTGTACAAGGCCCGATAATGTCCATAAATg < 5:396354/139‑1 (MQ=255) aaCAGCCAATAAAACCGCTTTTGAGAAACAAGCTCCCATACCTTCTGGCTGATGGCTTCAGGAGACGCGGCCGCATCCTCATTCAGCCAATACAGGATAATCGCGTACGTGTCCAAGGCCCGATAATGTCCATAAATGt > 6:200699/1‑139 (MQ=255) gCCAATAAAACCGCTTTAGAGAAACACGCTCCCATCCCTTCTGGCTGATGGCTTCAGGAGACGCGGCCGCACCCTCATTCAGCCAATACAGGATAATCGCGTACGTGTACAAGGCCCGATAATGTCCATAAATGTCCCg < 8:368142/139‑1 (MQ=255) aaaaCCGCTTTTGAGAAACACGCCCCCAACCCTTCTGGCTGAGGGCTTCCGGAGCCGCGGCCGCATCCTCATTCAGCCAATACAGGATAATCGCGTACGTGTACAAGGCCCGATAATGTCCATAAATGTCCCGTTCAAt < 3:342623/139‑1 (MQ=255) cGCTTTTGAGAAACAAGCTCCCATACCTTCTGGCTGATGGCTTCAGGAGACGCGGCCGCATCCTCATTCAGCCAATACAGGATAATCGCGTACGTGTACAAGGCCCGATAATGTCCATAAATGTCCCGTTCAATCGGCg > 4:403257/1‑139 (MQ=255) cGCTTTTGAGAAACAAGCTCCCATACCTTCTGGCTGATGGCTTCAGGAGACGCGGCCGCATCCTCATTCAGCCAATACAGGATAATCGCGTACGTGTACAAGGCCCGATAATGTCCATAAATGTCCCGTTCAATCGGCg > 3:351214/1‑139 (MQ=255) cTTTTGAGAAACAAGCTCCCATACCTTCTGGCTGATGGCTTCAGGAGACGCGGCCGCATCCTCATTCAGCCAATACAGGATAATCGCGTACGTGTACa < 7:58707/98‑1 (MQ=255) cTTTTGAGAAACAAGCTCCCATACCTTCTGGCTGATGGCTTCAGGAGACGCGGCCGCATCCTCATTCAGCCAATACAGGATAATCGCGTACGTGTACa > 8:58707/1‑98 (MQ=255) aaCAAGCTCCCATACCTTCTGGCTGATGGCTTCCGGAGACGCGGCCGCATCCTCATTCAGCCAATACAGGATAATCGCGTACGTGTACAAGGCCCGATAATGTCCATAAATGTCCCGTTCAATCGGCGTTAAATTcaca < 8:351984/139‑1 (MQ=255) acacGCCCCAATCCCTTCGGCCTCATCGCTTAGGGCGCCGCGGCCGCATCCTCATTCAGCCAATACAGGATTATCGCGTACGAGTACAAGGCCCGATAATGTCCATAAATGTCCCGTTCAATCGGCGTTAAATTCACAt < 6:61128/139‑1 (MQ=255) ccATACCTTCTGGCTGATGGCTTCAGGAGCCGCGGCCGCATCCTCATTCAGCCAATACAGGATAATCGCGTCCGTGTACAAGGCCCGCTAATGTCCATAAATGTCCCGTTCAATCGGCGTTAAATTCACATGGATCg > 4:297783/1‑137 (MQ=255) gaCCTTCTGGCTCCTGGCTGCCGCACACGCGGCCGCATCCTCAATCAGCCAATACACGATAATCGCGAACGTCTACAAGGCCCGATAATGTCCATAAATGTCCCGTTCAATCGGCGTTAAAATCACATGGATCGGAGcc < 3:252306/138‑1 (MQ=255) tCTGGCTGATGGCTTCAGGAGACGCGGCCGCATCCTCATTCAGCCAATACAGGATAATCGCGTACGTGTACAAGGCCCGATAATGTCCATAAATGTCCCGTTCAATCGTCGTTAAATTCACATGGATCGGAGCcagcag > 8:28475/1‑139 (MQ=255) tCTGGCTGATGGCTTCAGGAGACGCGGCCGCATCCTCATTCAGCCAATACAGGATAATCGCGTACGTGTACAAGGCCCGATAATGTCCATAAATGTCCCGTTCAATCGGCGTTAAATTCACATGGATCGGAGCcagcag > 3:301032/1‑139 (MQ=255) tCTGGCTGATGGCTTCAGGAGACGCGGCCGCATCCTCATTCAGCCAATACAGGATAATCGCGTACGTGTACAAGGCCCGATAATGTCCATAAATGTCCCGTTCAATCGGCGTTAAATTCACATGGATCGGAGCcagcag > 4:323746/1‑139 (MQ=255) tCTGGCTGATGGCTTCAGGAGACGCGGCCGCATCCTCATTCAGCCAATACAGGATAATCGCGTACGTGTACAAGGCCCGATAATGTCCATAAATGTCCCGTTCAATCGGCGTTAAATTCACATGGATCGGAGCcagcag > 8:104605/1‑139 (MQ=255) tCTGGCTGATGGCTTCAGGAGACGCGGCCGCATCCTCATTCAGCCAATACAGGATAATCGCGTACGTGTACAAGGCCCGATAATGTCCATAAATGTCCCGTTCAATCGGCGTTAAATTCACATGGATCGGAGCcagcag > 1:293217/1‑139 (MQ=255) tCTGGCTGATGGCTTCAGGAGACGCGGCCGCATCCTCATTCAGCCAATACAGGATAATCGCGTACGTGTACAAGGCCCGATAATGTCCATAAATGTCCCGTTCAATCGGCGTTAAATTCACATGGATCGGAGCca < 3:390248/135‑1 (MQ=255) tCTGGCTGATGGCTTCAGGAGACGCGGCCGCATCCTCATTCAGCCAATACAGGATAATCGCGTACGTGTACAAGGCCCGATAATGTCCATAAATGTCCCGTTCAATCGGCGTTAAATTCACATGGATCGGAGCca > 4:390248/1‑135 (MQ=255) tGGCTGATGGCTTCAGGAGACGCGGCCGCATCCTCATTCAGCCAATACAGGATAATCGCGTACGTGTACAAGGCCCGATAATGTCCATAAATGTCCCGTTCAATCGGCGTTAAATTAACATGGATCGGAGCCAccagca > 7:196641/1‑139 (MQ=255) tGGCTGATGGCTTCAGGAGACGCGGCCGCATCCTCATTCAGCCAATACAGGATAATCGCGTACGTGTACAAGGCCCGATAATGTCCATAAATGTCCCGTTCAATCGGCGTGAAATTCACATGGATCGGCGCCAgccgca > 3:216518/1‑139 (MQ=255) tGGCTGATGGCTTCAGGAGACGCGGCCGCATCCTCATTCAGCCAATACAGGATAATCGCGTACGTGTACAAGGCCCGAGAATGTCCATAAATGTACCGTTCAATCGGCGTTAAATTAACATGGATCGGAGCCAgcagca > 3:131634/1‑139 (MQ=255) tGGCTGATGGCTTCAGGAGACACGGCCGCATCCTCATTCAGCCAATACAGGATAATCGCGTACGTGTACAAGGCCCGATa > 1:370925/1‑80 (MQ=255) tGGCTGATGGCTTCAGGAGACACGGCCGCATCCTCATTCAGCCAATACAGGATAATCGCGTACGTGTACAAGGCCCGATa < 2:370925/80‑1 (MQ=255) gCTGATGGCTTCAGGAGACGCGGCCGCATCCTCATTCAGCCAATACAGGATAATCGCGTACGTGTACAAGGCCCGATAATGTCCATAAATGTCCCGTTCAATCGGCGTTAAATTCACATGGATCGGAGCCagcagc < 7:104605/136‑1 (MQ=255) gCTGATGGCTTCAGGAGACGCGGCCGCATCCTCATTCAGCCAATACAGGATAATCGCGTACGTGTACAAGGCCCGATAATGTCCATAAATGTCCCGTTCAATCGGCGTTAAATTCACATGGATCGGAGCCAGCAGcac > 1:66505/1‑138 (MQ=255) gCTGAGGGGTTCAGTAGACGCGGCCGCATCCTCATTCAGCCAATACAGGATAATCGCGTACGTGTACAAGGCCCGATAATGTCCATAAATGTCCCGTTCAATCGGCGTTAAATTCACATGGATCGGAGCCAGCAGcaca < 8:248701/139‑1 (MQ=255) ctGGCTCCAGGAGACGCGGCCGCATCCTCATTCAGCCAATACAGGATAATCGCGTACGTGTACAAGGCCCGATAATGTCCATAAATGTCCCGTTCAATCGGCGTTAAATTCACATGGATCGGAGCCAGCAGCACATCct < 3:373184/138‑1 (MQ=255) attgCTTCAGGAGACGCGGCCGCATCATCATTCATCCAATACAGGATAATCGCGTACGTGTAGAAGGCCCGATAATGTCCATAAATGTACCGTTCAGTCGGCGTTAAATTCACATGGATCGGAGACAGCAGCAGATCct > 8:232720/4‑139 (MQ=255) aTGGCTTCAGGAGACGCGGCCGCATCCTCTTTCAGCCAATACAGGATAAACGCGTACGTGTACAAGGCCGGATAATGTCCATAAATGTCCCGTTCAATCGGCGTTAAATTCACATGGATCGGAGCCAGCAGCACATc > 2:217838/1‑137 (MQ=255) aTGGCTTCAGGAGACGCGGCCGCATCCTCATTCAGCCAATACAGGATAATCGCGTACGTGTACAAGGCCCGATAATGTCCATAAATGTCCCGTTCAATCGGCGTTAAATTCACATGGATCGGAGCCAGCAGCACATCct > 5:197178/1‑139 (MQ=255) aTGGCTTCAGGAGACGCGGCCGCATCCTCATTCAGCCAATACAGGATAATCGCGTACGTGTACAAGGCCCGATAATGTCCATAAATGTCCCGTTCAATCGGCGTTAAATTCACATGGATCGGAGCCAGCAGCACATCct > 5:354036/1‑139 (MQ=255) aTGGCTTCAGGAGACGCGGCCGCATCCTCATTCAGCCAATACAGGATAATCGCGTACGTGTACAAGGCCCGATAATGTCCATAAATGTCCCGTTCAATCGGCGTTAAATTCACATGGATCCGAGCCAGCAGCCCAACct > 8:139338/1‑139 (MQ=255) aCGCGGCCGCATCCTCATTCAGCCAATACAGGATAATCGCGTACGTGTACAAGGCCCGATa < 4:318308/61‑1 (MQ=255) aCGCGGCCGCATCCTCATTCAGCCAATACAGGATAATCGCGTACGTGTACAAGGCCCGATa > 3:318308/1‑61 (MQ=255) aCGCGGCCGCATCCTCATTCAGCCAATACAGGATAATCGCGTACGTGTACAAGGCCCGATAATGTCCATAAATGTCCCGTTCAATCGGCGTTAAATTCACATGGATCGGAGCCAGCAGCACATCCTGCTGAAACACATc > 7:258493/1‑139 (MQ=255) aCGCGGCCGCATCCTCATTCAGCCAATACAGGATAATCGCGTACGTGTACAAGGCCCGATAATGTCCATAAATGTCCCGTTCAATCGGCGTTAAATTCACATGGATCGGAGCCAGCAGCACATCCTGCTGAAACACATc > 5:157504/1‑139 (MQ=255) aCGCGGCCGCATCCTCATTCAGCCAATACAGGATAATCGCGTACGTGTACAAGGCCCGATAATGTCCATAAATGTCCCGTTCAATCGGCGTTAAATTCAAAAGGATCGGAGCCAGCAGCACATCCTGCTGAAACACCTc > 2:32268/1‑139 (MQ=255) | GCCTCTCTCGTTCGAACCACCCGCCTGTCGATTTGCTTCTCCTTCTCTCGCTCTCCTGGCACAGCTTTTCCAAACAGCCAATAAAACCGCTTTTGAGAAACAAGCTCCCATACCTTCTGGCTGATGGCTTCAGGAGACGCGGCCGCATCCTCATTCAGCCAATACAGGATAATCGCGTACGTGTACAAGGCCCGATAATGTCCATAAATGTCCCGTTCAATCGGCGTTAAATTCACATGGATCGGAGCCAGCAGCACATCCTGCTGAAACACATC > BS168_reference_genome/4093221‑4093495 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |