| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | BS168_reference_genome | 4,153,115 | G→T | 100% | L192I (CTT→ATT) | walK ← | two‑component sensor histidine kinase [YycG] |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | BS168_reference_genome | 4,153,115 | 0 | G | T | 96.9% | 194.7 / ‑0.2 | 64 | L192I (CTT→ATT) | walK | two‑component sensor histidine kinase [YycG] |
| Reads supporting (aligned to +/- strand): ref base G (1/1); new base T (25/36); total (27/37) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.04e-01 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
TCATGTCCATACTTTTTAACCTTCCTCGAGAAATTCCCTTTCGCAAGCTCCATCGCCTGCTTTCGCATATCTGAAAGCGGGTGGGTAATGGTTCTTGCCAGAAAAATACCGAGAAGAGCCGTCAAAACAAGTGCCAAACCTGTGCCGGACGCCAGTATCGTGTTGATCGTCTTCATTTGATTAAAGACATCTTCCATACTCGCGACAACGTAGATCGCGCCCACAACCTCTTGGTTTTCAGTCATGACGGGCTTCG > BS168_reference_genome/4152985‑4153240 | tcatGTCCATACTTTTTAACCTTCCTCGAGAAATTCCCTTTCGCAAGCTCCATCGCCTGCTTTCGCATATCTGAAAGCGGGTGGGTAATGGTTCTTGCCAGAAAAATACCGAGAAGAGCCGTCAAAACAATTGCCAAAc > 8:84873/1‑139 (MQ=255) attcTTTTTAACCTTCCTCGAGAAATTCCCTTTCGCAAGCTCCATAGACTGGTTTCTCATATCTGAAAGCGGGTGGGTAATGGTTCTTGCCAGAAAAATACCGAGAAGAGCCGTCAAAACAATTGCCAAACCTGTGCCg < 5:330600/136‑1 (MQ=255) aTACTTTTTAACCTTCCTCGAGAAATTCCCTTTCGCAAGCCCCATCGCCTGCTTTCGCATATCTGAAAGCGGGTGGGTAATGGGTCTTGCCAGAAAAATACCGAGAAGAGCCGTCAAAACAATTGCCAAACCTGTGCCg < 5:43901/139‑1 (MQ=255) cTTTTTAACCTTCCTCGAGAAATTCCCTTTCGCAAGCTCCATCGCCTGCTTTCGCATATCTGAAAGCGGGTGGGTAATGGTTCTTGCCAGAAAAATACCGAGAAGAGCCGTCAAAACAATTGCCAAACCTGTGCCGGa < 6:175220/138‑1 (MQ=255) cTTTTTAACCTTCCTCGAGAAATTCCCTTTCGCAAGCTCCATCGCCTGCTTTCGCATATCTGAAAGCGGGTGGGTAATGGTTCTTGCCAGAAAAATACCGAGAAGAGCCGTCAAAACAATTGCCAAACCTGTGCCGGa < 2:282401/138‑1 (MQ=255) tttAACCTTCCTCGAGAAATTCCCTTTCGCAAGCTCCATCGCCTGCTTTCGCATATCTGAAAGCGGGTGGGTAATGGTTCTTGCCAGAAAAATACCGAGAAGAGCCGTCAAAACAATTGCCAAACCTGTGCCGGACg > 3:90155/1‑137 (MQ=255) tttAACCTTCCTCGAGAAATTCCCTTTCGCAAGCTCCATCGCCTGCTTTCGCATATCTGAAAGCGGGTGGGTAATGGTTCTTGCCAGAAAAATACCGAGAAGAGCCGTCAAAACAATTGCCAAACCTGTGCCGGACg < 4:90155/137‑1 (MQ=255) tttAACCTTCCTCGAGAAATTCCCTTTCGCAAGCTCCATCGCCTGCTTTCGCATATCTGAAAGCGGGTGGGTAATGGTTCTTGCCAGAAAAATACCGAGAAGAGCCGTCAAAACAATTGCCAAACCTGTGCCGGACGc < 5:369573/138‑1 (MQ=255) tttAACCTTCCTCGAGAAATTCCCTTTCGCAAGCTCCATCGCCTGCTTTCGCATATCTGAAAGCGGGTGGGTAATGGTTCTTGCCAGAAAAATACCGAGAAGAGCCGTCAAAACAATTGCCAAACCTGTGCCGGACGc < 2:394979/138‑1 (MQ=255) tttAACCTTCCTCGAGAAATTCCCTTTCGCAAGCTCCATCGCCTGCTTTCGCATATCTGAAAGCGGGTGGGTAATGGTTCTTGCCAGAAAAATACCGAGAAGAGCCGTCAAAACAATTGCCAAACCTGTGCCGGACGc > 6:369573/1‑138 (MQ=255) tttAACCTTCCTCGAGAAATTCCCTTTCGCAAGCTCCATCGCCTGCTTTCGCATATCTGAAAGCGGGTGGGTAATGGTTCTTGCCAGAAAAAAACCGAGAAGAGCCGTCAAAACAATTGCCAAAACTGTgccggccggc > 2:404733/1‑137 (MQ=255) tttAACCTTCATCGAGAAATTCCCTTTCGCAAGCTCCATCGCCTGCTTTAGCATATCTGAAAGCGGGTGGGTAATGGTTCTTGCCAGAAAAATACCGAGAAGAGCCGTCAAAACAATTGCCAAACCTGTGCAGGACGc > 1:6784/1‑138 (MQ=255) aCCTTCCTCGAGAAATTCCCTTTCGCAAGCTCCATCGCCTGCTTTCGCATTTCTGAAAGCGGGTGGGTAATGGTTATTGCCAGAAAAATACCGAGAAGAGCCGTCAAAACAATTGCCAAACCTGTGCCGGACGCCAGTa < 4:124736/139‑1 (MQ=255) aCCTTCCTCGAGAAATTCCCTTTCGCAAGCTCCATCGCCTGCTTTCGCATATCTGAAAGCGGGTGGGTAATGGTTCTTGCCAGAAAAATACCGAGAAGAGCCGTCAAAACAATTGCCAAACCTGTGCCGGACGCCAGTa < 7:243676/139‑1 (MQ=255) ccTTCCTCGAGAAATTCCCTTTCGCAAGCTCCATCGCCTGCTTTCGCATATCTGAAAGCGGGTGGGTAATGGTTCTTGCCAGAAAAATACCGAGAAGAGCCGTCAAAACAATTGCCAAACCTGTGCCGGACGCCAGTAt < 1:319294/139‑1 (MQ=255) ccTTCCTCGAGAAATTCCCTTTCGCAAGCTCCATCGCCTGCTTTCGCATATCTGAAAGCGGGTGGGTAATGGTTCTTGCCAGAAAAATACCGAGAAGAGCCGTCAAAACAATTGCCAAACCTGTGCCGGACGCCAGTAt < 6:195946/139‑1 (MQ=255) tCCTCGAGAAATTCCCTTTCGCAAGCTCCATCGCCTGCTTTCGCATATCTGAAAGCGGGTGGGTAATGGTTCTTGCCAGATAAATACCGAGAAGAGCCGTCAAAACAATTGCCAAACCTGTGCCGGACGCCAGTTTCgt > 6:315636/1‑139 (MQ=255) tCCTCGAGAAATTCCCTTTCGCAAGCTCCATCGCCTGCTTTCGCATATCTGAAAGCGGGTGGGTAATGGTTCTTGCCAGAAAAATACCGAGAAGAGCCGTCAAAACAATTGCCAAACCTGTGCCg > 3:76048/1‑125 (MQ=255) tCCTCGAGAAATTCCCTTTCGCAAGCTCCATCGCCTGCTTTCGCATATCTGAAAGCGGGTGGGTAATGGTTCTTGCCAGAAAAATACCGAGAAGAGCCGTCAAAACAATTGCCAAACCTGTGCCg < 4:76048/125‑1 (MQ=255) tCCTCGAGAAATTCCCTTTCGCAAGCTCCATCGCCTGCTTTCGCATATCTGAAAGCGGGTGGGTAATGGTTCTTGCCAGAAAAATACCGAGAAGAGCCGTCAAAACAATTGCCAAACCTGTGCCGGACGCCAGTATCgt > 7:202697/1‑139 (MQ=255) tCCTCGAGAAATTCCCTTTCGCAAGCTCCATCGCCTGCTTTCGCATATCTGAAAGCGGGTGGGTAATGGTTCTTGCCAGAAAAATACCGAGAAGAGCCGTCAAAACAATTGCCAAACCTGTGCCGGACGCCAGTATCgt > 3:127192/1‑139 (MQ=255) cTCGAGAAATTCCCTTTCGCAAGCTCCATCGCCTGCTTTCGCATATCTGAAAGCGGGGGGGTAATGGTTCTTGCCAGAAAAATACCGAGAAGAGCCGTGCAAACCATTCCAAAACCTGTGCCGGCCGCCACTAACgggg > 2:190427/1‑136 (MQ=255) gagaAATTCCCTTTCGCAAGCTCCATCGCCTGCTTTCGCATATCTGAAAGCGGGTGGGTAATGGTTCTTGCCAGAAAAATACCGAGAAGAGCCGTCAAAACAATTGCCAAACCTGTGCCGGACGCCAGTATCGTGTTGa < 4:195736/139‑1 (MQ=255) tCGCAAGCTCCATCGCCTGCTTTCGCATATCTGAAAGCGGGTGGGTAATGGTTCTTGCCAGAAAAATACCGAGAAGAGCCGTCAAAACAATTGCCAAACCTGTGCCGGACGCCAGTATCGTGTTGATCGTCTTCATTTg > 4:330223/1‑139 (MQ=255) aaGCTCCATCGCCTGCGTTCGCATATCTGAAAGCGGGTGGGTAATGGTTCTTGCCAGAAAAATACCGAGAAGAGCCGTCAAAACAATTGCCAAACCTGTGCCGGACGCCAGTATCGTGTTGATCGTCTTCATTTGATTa < 6:28388/139‑1 (MQ=255) cATCGCCTGCTTTCGCATATCTGAAAGCGGGTGGGTAATGGTTCTTGCCAGAAAAATACCGAGAAGAGCCGTCAAAACAATTGCCAAACCTGTGCCGGACGCCAGTATCGTGTTGATCGTCTTCATTTGATTAAAGa < 7:9710/137‑1 (MQ=255) cATCGCCTGCTTTCCCATATCTGAAAGCGGGTGGGTAATGGTTCTTGCCAGAAAAATACCGAGAAGAGCCGTCAAAACAATTGCCAAACCTGTGCCGGACGCCAGTATCGTGTTGATCGTCTTCATTTGATTAAAGAc < 6:207400/138‑1 (MQ=255) aTCGCCTGCTTTCGCATATCTGAAAGCGGGTGGGTAATGGTTCTTGCCAGAAAAATACCGAGAAGAGCCGTCAAAACAATTGCCAAACCTGTGCCGGACGCCAGTATCGTGTTGATCGTCTTCATTTGATTAAAGACAt < 7:281221/139‑1 (MQ=255) aTCGCCTGCTTTCGCATATCTGAAAGCGGGTGGGTAATGGTTCTTGCCAGAAAAATACCGAGAAGAGCCGTCAAAACAATTGCCAAACCTGTGCCGGACGCCAGTATCGTGTTGATCGTCTTCATTTGATTAAAGACAt < 2:252589/139‑1 (MQ=255) tCGCCTGCTTTCGCATATCTGAAAGCGGGTGGGTAATGGTTCTTGCCAGAAAAATACCGAGAAGAGCCGTCAAAACAATTGCCAAACCTGTGCCGGACGCCAGTATCGTGTTGATCGTCTTCATTTGATTAAAGACATc > 3:160477/1‑139 (MQ=255) cTTTCGCATATCTGAAAGCGGGTGGGTAATGGTTCTTGCCAGAAAAATACCGAGAAGAGCCGTCAAAACAATTGCCAAACCTGTGCCGGACGCCAGTATCGTGTTGATCGTCTTCATTTGATTAAAGACa < 8:195994/130‑1 (MQ=255) cTTTCGCATATCTGAAAGCGGGTGGGTAATGGTTCTTGCCAGAAAAATACCGAGAAGAGCCGTCAAAACAATTGCCAAACCTGTGCCGGACGCCAGTATCGTGTTGATCGTCTTCATTTGATTAAAGACa > 7:195994/1‑130 (MQ=255) tCGCATATCTGAAAGCGGGTGGGTAATGGTTCTTGCCAGAAAAATACCGAGAAGAGCCGTCAAAACAATTGCCAAACCTGTGCCGGACGCCAGTATCGTGTTGATCGTCTTCATTTGATTAAAGACATCTTCCATACt < 7:84873/138‑1 (MQ=255) tCGCATATCTGAAAGCGGGTGGGTAATGGTTCTTGCCAGAAAAATACCGAGAAGAGCCGTCAAAACAATTGCCAAACCTGTGCCGGACGCCAGTATCGTGTTGATCGTCTTCATTTGATTAAAGACATCTTCCATACt < 8:264589/138‑1 (MQ=255) caatatCTGATAGCGGGTGGGTATTGGTTCTTGCCAGAAAATTACCGAGAAGAGCCGTCAAAACAATTGCCAAACCTGTGCCGGACGCCAGTAGCGTGTTGATCGTCTTCATTTGATTAAAGACATCTTCCATACTcg < 1:190427/136‑1 (MQ=255) cATATCTGAAAGCGGGTGGGTAATGGTTCTTGCCAGAAAAATACCGAGAAGAGCCGTCAAAACAATTGCCAAACCTGTGCCGGACGCCAGTATCGTGTTGATCGTCTTCATTTGATTAAAGACATCTTCCATACTcgcg < 4:256959/139‑1 (MQ=255) cATATCTGAAAGCGGGTGGGTAATGGTTCTTGCCAGAAAAATACCGAGAAGAGCCGTCAAAACAATTGCCAAACCTGTGCCGGACGCCAGTATCGTGTTGATCGTCTTCATTTGATTAAAGACATCTTCCATACTcgcg < 4:160477/139‑1 (MQ=255) cATATCTGAAAGCGGGTGGGTAATGGTTCTTGCCAGAAAAATACCGAGAAGAGCCGTCAAAACAATTGCCAAACCTGTGCCGGACGCCAGTATCGTGTTGATCGTCTTCATTTGATTAAAGACATCTTCCATACTcgcg < 4:127192/139‑1 (MQ=255) atatCTGAATGCGGGTGGGTAATGGTTCTTGCCAGAAAAATACCGAGAAGAGCCGTCAAAACAATTGCCAAACCTGTGCCGGACGCCAGTATCGTGTTGATCGTCTTCATTTGATTAAAGACATCTTCCATACTCGCGa < 6:296451/139‑1 (MQ=255) ggtgggtAATGGTTCTTGCCAGAAAAATACCGAGAAGAGCCGTCAAAACAAATGCCAAACCACTGCCGGCCGCCACTATCGTGGTGATCGTCGTGAATTGATTAAAGACCTCTTCCAAACTCGCGACAAAGTAAAAcgc > 3:212106/1‑139 (MQ=255) gtgggtAATGGTTCTTGCCAGAAAAATACCGAGAAGAGCCGTCAAAACAATTGCCAAACCTGTGCCGGACGCCAGTa > 2:216939/1‑77 (MQ=255) gtgggtAATGGTTCTTGCCAGAAAAATACCGAGAAGAGCCGTCAAAACAATTGCCAAACCTGTGCCGGACGCCAGTa < 1:216939/77‑1 (MQ=255) gtgggtAATGGTTCTTGCCAGAAAAATACCGAGAAGAGCCGTCAAAACAATTGCCAAACCTGTGCCGGACGCCAGTATCGTGTTGATCGTCTTCATTTGATTAAAGACa > 6:404590/1‑109 (MQ=255) gtgggtAATGGTTCTTGCCAGAAAAATACCGAGAAGAGCCGTCAAAACAATTGCCAAACCTGTGCCGGACGCCAGTATCGTGTTGATCGTCTTCATTTGATTAAAGACa < 5:404590/109‑1 (MQ=255) gtgggtAATGGTTCTTGCCAGAAAAATACCGAGAAGAGCCGTCAAAACAATTGCCAAACCTGTGCCGGACGCCAGTATCGTGTTGATCGTCTTCATTTGATTAAAGACATCTTCCATACTCGCGACAACGTAGATc < 8:314581/136‑1 (MQ=255) gtgggtAATGGTTCTTGCCAGAAAAATACCGAGAAGAGCCGTCAAAACAATTGCCAAACCTGTGCCGGACGCCAGTATCGTGTTGATCGTCTTCATTTGATTAAAGACATCTTCCATACTCGCGACAACGTAGATc > 7:314581/1‑136 (MQ=255) tgggtAATGGTTCTTGCCAGAAAAATACCGAGAAGAGCCGTCAAAACAAGTACCAAACCTGTGCCGGACGCCAGTATCGTGTTGATCGTCTTCATTTGATTAAAGAc < 4:35166/107‑1 (MQ=255) tgggtAATGGTTCTTGCCAGAAAAATACCGAGAAGAGCCGTCAAAACAAGTACCAAACCTGTGCCGGACGCCAGTATCGTGTTGATCGTCTTCATTTGATTAAAGAc > 3:35166/1‑107 (MQ=255) gggtAATGGTTCTTGCCAGAAAAATCCCGTGAAGAGCCGTCAAAACAATTGCCAAACCTGTGCCGGACGCCAGTATCGTGTTGATCGTCTTCATTTGATTAAAGACATCTTCCATACTCGCGACAACGTAGATCGCGcc < 3:333099/139‑1 (MQ=255) aTGGTTCTTGCCAGAAAAATACCGAGAAGAGCCGTCAAAACAATTGCCAAACCTGTGCCGGACGCCAGTATCGTGTTGATCGTCTTCATTTGATTAAAGACATCTTCCATACTCGCGACAACGTAGATCGCGCCcaca < 3:182599/138‑1 (MQ=255) aTGGTTATTGCCATAAAAATACCTATAAGAGACTTCAAAAAAATTGCAAAACCTGGGCCGGACGCCAGTATCGTGTTGATCGTCTTCATTTGATTAAAGAAATCTTCCATACTCGCGACAACGTAGATCGCGCCcaca < 4:212106/138‑1 (MQ=255) tGGTTCTTGCCAGAAAAATACCGAGAAGAGCCGTCAAAACAATTGCCAAACCTGTGCCCGACGCCAGTATCGTGTTTATCGTCTTGATTTGATTAAAGACCAGTTCCATACTAGCGACCACGTAGAACGCGCCCCCAAc > 6:63346/1‑139 (MQ=255) cTTGCCAGAAAAATACCGAGAAGAGCCGTCAAAACAATTGCCAAACCTGTGCCGGACGCCAGTATCGTGTTGATCGTCTTCATTTGATTAAAGACATCTTCCATACTCGCGACAACGTAGATCGCGCCCACAACCTCtt > 1:21845/1‑139 (MQ=255) cTTGCCAGAAAAATACCGAGAAGAGCCGTCAAAACAATTGCCAAACCTGTGCCGGACGCCAGTATCGTGTTGATCGTCTTCATTTGATTAAAGACATCTTCCATACTCGCGACAACGTAGATCGCGCCCACAACCGCtt > 5:39634/1‑139 (MQ=255) tGCCAGAAAAATACCGAGAAGAGCCGTCAAAACAATTGCCAAACCTGTGCCGGACGCCAGTATCGTGTTGATCGTCTTCATTTGATTAAAGACATCTTCCATACTCGCGACAACGTAGATCGCGCCCCCAACCTCTTgg > 2:264088/1‑139 (MQ=255) aGAAAAATACCGAGAAGAGCCGTCAAAACAATTGCCAAACCTGTGCCGGACGCCAGTATCGTGTTGATCGTCTTCATTTGATTAAAGACATCTTCCATACTCGCGACAACGTAGATCGCGCCCACAACCTCTTGGtttt < 3:406083/139‑1 (MQ=255) aGAAAAATACCGAGAAGAGCCGTCAAAACAATTGCCAAACCTGTGCCGGACGCCAGTATCGTGTTGATCGTCTTCATTTGATTAAAGACATCTTCCATACTCGCGACAACGTAGATCGCGCCCACAACCTCTTGGtttt < 7:188028/139‑1 (MQ=255) gAAAAATACCGAGAAGAGCCGTCAAAACAATTGCCAAACCTGTGCCGGACGCCAGTATCGGGTTGGTCGTCTTCATTTGATTAAATACATCTTCCATACTCGCGACAACGTAGATCGCGCCCACAACCGAGTGGtttt > 1:257518/1‑138 (MQ=255) aaaaaTACCGAGAAGAGCCGTCAAAACAATTGCCAAACCTGTGCCGGACGCCAGTATCGTGTTGATCGTCTTCATTTGATTAAAGACATCTTCCATACTCGCGACCACGTAGATCGCGCCCACAACCTCTTGGTTTTcc > 5:45599/1‑138 (MQ=255) aaaaaTACCGAGAAGAGCCGTCAAAACAATTGCCAAACCTGTGCCGGACCCCAGTATCGGGTTGATCGTCTTCATTTGATTAAAGACCTCTTCCATACTCGCGCCAACGTAGATCGCGCCCACACCCTCTTGGtttt > 8:403606/1‑137 (MQ=255) agaagaGCCGTCAAAACAATTGCCAAACCAGTGCCGGCCGCCAGTATCGTGTTGATCGTCGTCATTTGATTAAAGACATCTTCCATACTCGCCACAACGTAGCTCGCGCCCACAACCTCTTGGGTTTCAGTCATGACgg > 5:118078/1‑139 (MQ=255) agCCGTCAAAACAATTGCCAAACCTGTGCCGGACGCCAGTATCGTGTTGATCGTCTTCATTTGATTAAAGACATCTTCCATACTCGCGACAACGTAGATCGCGCCCACAACCTCTTGGTTTTCAGTCATGCCGGGCTTc > 1:215345/1‑139 (MQ=255) agCCGCCAAAACAATTGCCAAACCTGCGCCGGACGCCAGTATCGTGTTGATCGTCTTCATTTGATTAAAGACATCTTCCATACTCGCGACAACGTAGATCGCGCCCACAACCTCTTGGTTTTCAGTCATGACGGGCTTc < 1:404733/139‑1 (MQ=255) gCCGTCAAAACAATTGCCAAACCTGTGCCGGACGCCAGTATCGTGTTGATCGTCTTCATTTGATTAAAGACATCTTCCATACTCGCGACAACGTAGATCGCGCCCACAACCTCTTGGTTTTCAGTCATGACGGGCTTcg < 1:49646/139‑1 (MQ=255) | TCATGTCCATACTTTTTAACCTTCCTCGAGAAATTCCCTTTCGCAAGCTCCATCGCCTGCTTTCGCATATCTGAAAGCGGGTGGGTAATGGTTCTTGCCAGAAAAATACCGAGAAGAGCCGTCAAAACAAGTGCCAAACCTGTGCCGGACGCCAGTATCGTGTTGATCGTCTTCATTTGATTAAAGACATCTTCCATACTCGCGACAACGTAGATCGCGCCCACAACCTCTTGGTTTTCAGTCATGACGGGCTTCG > BS168_reference_genome/4152985‑4153240 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |