BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	pSIJ663_pHT315_araE_xy	121	G→C	5.3%	intergenic (–/‑2857)	– / → eryR	–/–

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	pSIJ663_pHT315_araE_xy	121	0	G	C	5.3%	38321.5 / 264.0	13391	intergenic (–/‑2857)	–/eryR	–/–
Reads supporting (aligned to +/- strand): ref base G (5680/6337); new base C (2/942); total (5702/7689)
Fisher's exact test for biased strand distribution p-value = 0.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 13586 total aligned reads displayed.

‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑TCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCTTGTTTTCGC  >  pSIJ663_pHT315_araE_xy/1‑259

tgataaatgcttcaataaTCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGAGTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAg                                                                                                                                            <  8:36097‑M1/121‑1 (MQ=255)

  ataaatgcttcaataaTCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCTGTAAAATTAGCAGTAACTTAATAGaa                                                                                                                                          <  4:19374‑M1/123‑1 (MQ=255)

  ataaatgcttcaataaTCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCTGTAACTTAATAGaa                                                                                                                                          <  2:36068‑M1/123‑1 (MQ=255)

  ataaatgcttcaataaTCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGaa                                                                                                                                          <  2:257454‑M1/123‑1 (MQ=255)

  ataaatgcttcaataaTCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGaa                                                                                                                                          <  5:2266‑M1/123‑1 (MQ=255)

  ataaatgcttcaataaTCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGaa                                                                                                                                          <  2:149783‑M1/123‑1 (MQ=255)

  ataaatgcttcaataaTCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGaa                                                                                                                                          <  5:316546‑M1/123‑1 (MQ=255)

  ataaatgcttcaataaTCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGaa                                                                                                                                          <  3:270997‑M1/123‑1 (MQ=255)

  ataaatgcttcaataaTCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGaa                                                                                                                                          <  5:346611‑M1/123‑1 (MQ=255)

  ataaatgcttcaataaTCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGAGGTAAAATTAGCAGTAACTTAATAGaa                                                                                                                                          <  4:326839‑M1/123‑1 (MQ=255)

   taaatgcttcaataaTCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGaa                                                                                                                                          >  5:259808‑M1/16‑138 (MQ=255)

       tgcttcaataaTCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGaa                                                                                                                                     >  1:311318‑M1/12‑139 (MQ=255)

       tgcttcaataaTCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGa                                                                                                                                      <  2:57736‑M1/127‑1 (MQ=255)

        gcttcaataaTCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATATAACGGaaa                                                                                                                                    <  7:224200‑M1/129‑1 (MQ=255)

        gcttcaataaTCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGaaa                                                                                                                                    <  8:102584‑M1/129‑1 (MQ=255)

        gcttcaataaTCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGaaa                                                                                                                                    <  7:301781‑M1/129‑1 (MQ=255)

            caataaTCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAATTGa                                                                                                                                 <  3:242484‑M1/132‑1 (MQ=255)

            caataaTCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGa                                                                                                                                 <  6:283122‑M1/132‑1 (MQ=255)

            caataaTCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGa                                                                                                                                 <  4:164537‑M1/132‑1 (MQ=255)

            caataaTCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGa                                                                                                                                 <  7:121221‑M1/132‑1 (MQ=255)

            caataaTCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGAGTTAAAATTAGCAGTAACTTAATAGAACGGAAATGa                                                                                                                                 <  1:154386‑M1/132‑1 (MQ=255)

                 atCCATCCTCCAACGTTGGAGATTTAGTTTTATGGCGCAAATATTAATGTTTCTTGTGAACCTTATAAAATTTTCGTTTATTTAATAGAAACATAGCGGTAAAATTAGCAGAAACTTAATAGAACGTAAATCAAAAAAg                                                                                                                           <  2:92097/138‑1 (MQ=255)

                  tCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGaa                                                                                                                                     >  8:165276/1‑128 (MQ=255)

                  tCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGc                                                                                                                          >  6:342505/1‑139 (MQ=255)

                  tCCATCCTCCAAAGTGGGAGAGTGAGTTTTATGTCCCAAATATTAATGTTTCTGGTGAACCTAATCAAATTTTCGTTGATTAAATAGAAACATAGCGGTAAAATTAGCAGAAACTTAATAGAACGGAAATGAAAAAAGc                                                                                                                          <  6:205836/139‑1 (MQ=255)

                    cATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAATTGAAAAAAGCCa                                                                                                                        <  7:78887/139‑1 (MQ=255)

                    cATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCa                                                                                                                        >  8:389743/1‑139 (MQ=255)

                    cATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCa                                                                                                                        <  3:382295/139‑1 (MQ=255)

                    cATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTATGCTGAACCTTATCAAATTTTCGTTTATTTAATATAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCa                                                                                                                        <  8:306541/139‑1 (MQ=255)

                    cATCCTCCAAAGTGGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTCAACCTTATCAAATTTTCGTTGATTAAATACAAACATAGCGGTAAAATTAGCAGTAACTAAATAGAACGCAAAGGAAAAAAGCCa                                                                                                                        <  4:126221/139‑1 (MQ=255)

                    aaTCCTCCAACGTTGTATTGTGAGTTTTTTGTCGCAAATATTATTGTTTCTGGTGAACCTTATCAAATTTTCTTTTATTTAATAGAAACATTGGGGTAAATTTAGCAGTAACTTATTAGAACGTAAATCAAAAAAGCCa                                                                                                                        <  8:250213/138‑1 (MQ=255)

                      tcctccAAAGTTTGAGAGTGATTTTTATGTCGAAAATATTAATGTTTCTGGTGAACCTTATAAAATTTTCTTTGATTTAATAGAAACATAGCGTTAAAATTAGCATTAACTTAATAGAACGGAAATTAAAAAAGCCAct                                                                                                                      <  6:145300/139‑1 (MQ=255)

                      tcctccAAAGTTGGAGTGTGAGTTTTATGTCGAAAATATTATTGTTTCTGGTGAACCTTATAAAATTTTCGTTGATTTAATACAAACATAGCGGTAAAATTAGCATTAACTTAATATACCGGAAATGAAAAAAGCCAct                                                                                                                      <  1:100045/139‑1 (MQ=255)

                      tcctccAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCa                                                                                                                        <  1:126066/137‑1 (MQ=255)

                      tcctccAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCAct                                                                                                                      >  3:63316/1‑139 (MQ=255)

                      tcctccAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCAct                                                                                                                      >  3:182434/1‑139 (MQ=255)

                      tcctccAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCAct                                                                                                                      >  8:192537/1‑139 (MQ=255)

                      tcctccAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCAct                                                                                                                      >  4:355520/1‑139 (MQ=255)

                      tcctccAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACATTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGCAAATGAAAAAAGCCAct                                                                                                                      <  1:221082/139‑1 (MQ=255)

                       cctccAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGATACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCActc                                                                                                                     >  1:407137/1‑139 (MQ=255)

                       cctccAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCActc                                                                                                                     >  1:252430/1‑139 (MQ=255)

                           cAAAGTTGGAGAGTGATTTTTATGTCGCAAATATTATTGTTTCTGGTGAACATTATAAAATTTTCGTTTATTTAATAGAAACATAGCGGTAAAATTAGCTGTAAATTAATAGAACGCAAATTAAAAAAGCCACtctc                                                                                                                   <  8:71939/137‑1 (MQ=255)

                           cAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCa                                                                                                                  >  2:396912/1‑138 (MQ=255)

                              aGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATg                                                                                                              <  3:30112/139‑1 (MQ=255)

                              aGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATg                                                                                                              <  3:331606/139‑1 (MQ=255)

                                   tagagTGAGTTTTATGTAGAAAATATTAATGTTTCTGGTGAACCTTATAAAATTTTCGTTTATTTAATAGAAAAATAGCGTTAAAATTAGCTGTAACTAAATAGAACGTAAATCAAAAACGCCACTCTCATATGCTAtt                                                                                                         <  4:227865/138‑1 (MQ=255)

                                   gagaGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTAtt                                                                                                         >  4:294773/1‑139 (MQ=255)

                                   gagaGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTAtt                                                                                                         >  4:387713/1‑139 (MQ=255)

                                   gagaGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTAtt                                                                                                         >  7:249604/1‑139 (MQ=255)

                                   gagaGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTAtt                                                                                                         >  6:176071/1‑139 (MQ=255)

                                        tgagtTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTa                                                                                                    >  7:19962/1‑139 (MQ=255)

                                          agtTTTATTTCGCAAATATTAATGTTTCTGGTTAACCTTATCAAATTTTCGTTTATTTAATAGAAACATAGCGGAAAAATTAGCAGTAACTTATTAGAACGCAAATGAAAAAAGCCACTCTCATATGCTATTGGCTAcc                                                                                                  <  7:144405/139‑1 (MQ=255)

                                          agtTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTAc                                                                                                   >  7:32450/1‑138 (MQ=255)

                                          agtTTTATGTAGAAAATATTAATGTTTGTGGTGAACCTTATAAAATTTTCTTTTATTTAATAGAAACATAGCGGTAAAATTAGCATTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTAcc                                                                                                  <  8:42860/139‑1 (MQ=255)

                                           gtTTTATGTCCAAAATATTATTGTTTCTGGTGAACCTAAACAAATTTTCGTTGATTAAATACAAACATAGCGTTAAAATTCCCAGTAACTAAAAACACCGGAAATTAAAAAAGCCACTCTCATATGCTATTGGCTAcc                                                                                                  <  4:44702/138‑1 (MQ=255)

                                             tttATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCTGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAATCCACTCTCATATGCTATTGGCTACCAAc                                                                                               >  6:406190/1‑139 (MQ=255)

                                                aTGTAGCAAATATTAATGTTTCTGTTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATCGAACGTAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCt                                                                                             <  5:402539/138‑1 (MQ=255)

                                                     gCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGc                                                                                        <  3:91568/138‑1 (MQ=255)

                                                      cAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCAc                                                                                                                       <  3:124903/106‑1 (MQ=255)

                                                         atatTAATTTTTCTGGTGAACCTTATCTATTTTTCGTTTATTTAATATAAACATAGGGGTAAAATTAGCATTAAATTAATAGAAGTGAAATTAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGaa                                                                                   <  5:5711/139‑1 (MQ=255)

                                                         atatTAATGTTTCTGGTGCACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGAAAAATTACACTTAACTAAATAGAACGGAAATCAAAAACGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGaa                                                                                   <  8:222820/139‑1 (MQ=255)

                                                         atatTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTTATTTAATAGAAACTTAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATTAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGaa                                                                                   <  4:261367/139‑1 (MQ=255)

                                                         atatTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTTATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATATAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGaa                                                                                   <  4:79433/139‑1 (MQ=255)

                                                         atatTAATCTTTCTGGTGAACCTTACAAATTTTTCGTGTTATTAATAGAAACATCCCGGTAAAATAACCAGTATCTTAATAGACCGCAACTCAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGaa                                                                                   <  6:244003/139‑1 (MQ=255)

                                                               aTGTTTCTGTTGAACATTATCAAATTTTCGTTGATTTAATAGAAAAAAAGCGGAAAAATTAGCAGAAAATAAATACAACGCAAATTAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACt                                                                              <  2:292951/138‑1 (MQ=255)

                                                               aTGTTTCTGGTTAACCTTACCAATTTTTCTTCGTTTTAATAGAAACATTGGGCTAAAATTACCAGTAAATTAATTTACATTAAATCTAAAACGCCACTCTGATATGCTATTGGCTACCAACCTTTAGCGAGAATGACt                                                                              <  2:224436/138‑1 (MQ=255)

                                                               aTGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACt                                                                              <  3:353891/138‑1 (MQ=255)

                                                                  tttCTGGCGAACATTATCAAATTTTCGTTTATTTAATACAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTaa                                                                           <  1:375152/138‑1 (MQ=255)

                                                                    tCTGGTGAACCTTATCAAATTTTCGTTTATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGaa                                                                                   <  1:342095/128‑1 (MQ=255)

                                                                    tCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATcc                                                                        >  7:402873/1‑139 (MQ=255)

                                                                    tCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATcc                                                                        >  7:275872/1‑139 (MQ=255)

                                                                    tCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATcc                                                                        >  1:60107/1‑139 (MQ=255)

                                                                    tCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATcc                                                                        >  6:68393/1‑139 (MQ=255)

                                                                        gTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTg                                                                      <  2:18570/137‑1 (MQ=255)

                                                                        gTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTg                                                                      <  7:348667/137‑1 (MQ=255)

                                                                        gTGAACCTTATAAAATTTTCGTTTATTTAATAGAAACATAGCGGTAAAATTAGCAGAAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTg                                                                      <  5:111967/137‑1 (MQ=255)

                                                                           aaCCTTATCAAATTTTCTTTTATTAAATACAAACATTGCGGTAAAATTATCAGTAACTTAATATAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAg                                                                 <  1:187038/139‑1 (MQ=255)

                                                                            acattATAAAATTTTGGTTTATTTAAAATAAACATAGCGGTAAAATTAGCTTTAACTTAATAGACCGTAAATCAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAg                                                                 <  6:113074/135‑1 (MQ=255)

                                                                            aCCTTATCAAATTTTCGTTGATTAAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAg                                                                 <  5:141067/138‑1 (MQ=255)

                                                                                tATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAg                                                                 <  1:283496/134‑1 (MQ=255)

                                                                                tATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCAt                                                             >  5:373616/1‑138 (MQ=255)

                                                                                       ttttCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACt                                                     >  3:209029/1‑139 (MQ=255)

                                                                                        tttCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATAc                                                           >  5:195990/1‑132 (MQ=255)

                                                                                         ttCGTTTATTTAATACAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGTAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACtt                                                    <  6:92384/138‑1 (MQ=255)

                                                                                         ttCGTTGATTTAATAGAAACATAGGTGTAAAATTAGCAGAAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACtt                                                    <  8:280139/138‑1 (MQ=255)

                                                                                         ttCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACtt                                                    <  7:52729/138‑1 (MQ=255)

                                                                                         tgcGTTGATTAAATAGAAAAATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACtt                                                    <  8:133666/136‑1 (MQ=255)

                                                                                                           aaaTACCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATCAAAAAAGACACTCAAATATACTATTCTATAACAACATTTAGAGAGAATGAATTAATCATTTAAAGACAAACAGAACTTAGAATTATAAGAGtcgca                                  >  8:332477/3‑137 (MQ=255)

                                                                                                           aCATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGcc                                  >  6:313001/1‑138 (MQ=255)

                                                                                                               aGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACtt                             >  3:407220/1‑139 (MQ=255)

                                                                                                                gCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACtt                             >  3:155514/1‑138 (MQ=255)

                                                                                                                 cGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTc                            >  2:121346/1‑138 (MQ=255)

                                                                                                                    tAAAATTAGCATTAACTTAATAGACCGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAAt                        <  1:90607/139‑1 (MQ=255)

                                                                                                                     aaaaTTAGCAGTAACTAAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAAt                        <  7:377196/138‑1 (MQ=255)

                                                                                                                           aGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTAt                 >  2:322731/1‑139 (MQ=255)

                                                                                                                                tAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGcc            <  5:172122/139‑1 (MQ=255)

                                                                                                                                  aCTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTTGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCtt          >  7:175795/1‑139 (MQ=255)

                                                                                                                                  aCTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGTCGCCAACTTCAAATAAGTTATTTTCCtt          >  5:286587/1‑139 (MQ=255)

                                                                                                                                  aCTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCtt          >  5:84704/1‑139 (MQ=255)

                                                                                                                                  aCTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCtt          >  6:372175/1‑139 (MQ=255)

‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑TCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCTTGTTTTCGC  >  pSIJ663_pHT315_araE_xy/1‑259

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`