BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	pSIJ663_pHT315_araE_xy	3,729	T→G	7.2%	intergenic (+14/‑142)	eryR → / → ampR	–/–

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	pSIJ663_pHT315_araE_xy	3,729	0	T	G	7.2%	27909.9 / inf	9422	intergenic (+14/‑142)	eryR/ampR	–/–
Reads supporting (aligned to +/- strand): ref base T (701/7822); new base G (847/24); total (1549/7873)
Fisher's exact test for biased strand distribution p-value = 0.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 9951 total aligned reads displayed.

TGTTTACTAAAAATCAGTTTCATCAAGCAATGAAACA‑CGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTAC‑TAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGTATCGGGGTACATTGAAAAAGGAAGAGTAT  >  pSIJ663_pHT315_araE_xy/3591‑3872

 gTTTACTAAAAATCTGTTTCATCAAGCAATGAAACA‑CGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑tt                                                                                                                                                 >  6:139339/1‑139 (MQ=255)

  tttACTAAAAATCAGTTTCATCAAGCAATGAAACA‑CGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑ttt                                                                                                                                                >  8:68827/1‑139 (MQ=255)

  tttACTAAAAATCAGTTTCATCAAGCAATGAAACA‑CGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑ttt                                                                                                                                                >  7:135921/1‑139 (MQ=255)

  tttACTAAAAATCAGTTTCATCAAGCAATGAAACA‑CGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑ttt                                                                                                                                                >  1:333089/1‑139 (MQ=255)

    tACTAAAAATCAGTTTCATCAAGCAATGAAACA‑CGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTTTTTAACGGGAGGAAATAATTCTATCAGTCGC‑tgttt                                                                                                                                              >  4:27932/1‑139 (MQ=255)

           aaTCAGTTTCATCAAGCAATGAAACA‑CGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCGTTTTTTTAAtt                                                                                                                                        >  6:263203/1‑139 (MQ=255)

            aTCAGTTTCATCAAGCAATGAAACA‑CGCCAAAGTGAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑GTTTTTTAATTTg                                                                                                                                      >  6:294932/1‑139 (MQ=255)

            aTCAGTTTCATCAAGCAATGAAACA‑CGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAAtttt                                                                                                                                      >  5:375012/1‑138 (MQ=255)

            aTCAGTTTCATCAAGCAATGAAACA‑CGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTg                                                                                                                                      <  2:43672/139‑1 (MQ=255)

             tCAGTTTCATCAAGCAATGAAACA‑CGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTgg                                                                                                                                     <  4:338618/139‑1 (MQ=255)

               aGTTTCATCAAGCAATGAAACA‑CGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGATGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGa                                                                                                                                    <  7:8844/138‑1 (MQ=255)

               aGTTTCATCAAGCAATGAAACA‑CGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGa                                                                                                                                    <  7:63895/138‑1 (MQ=255)

                 tttCATCAAGCAATGAAACA‑CGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAg                                                                                                                                 >  7:324701/1‑139 (MQ=255)

                     atcaAGAAATGAAAAA‑CGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTa                                                                                                                              <  4:148275/138‑1 (MQ=255)

                     atcaACCAATGAAACA‑CGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTa                                                                                                                              <  1:250953/138‑1 (MQ=255)

                      tcaAGCAATTAAACA‑CGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTaca                                                                                                                            <  1:191675/139‑1 (MQ=255)

                      tcaAGCAATGAAACA‑CGCCAAAGTAAAGAATTTAAGTTCCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTaca                                                                                                                            <  3:158868/139‑1 (MQ=255)

                      tcaAGCAATGAAACA‑CGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTaca                                                                                                                            <  7:348614/139‑1 (MQ=255)

                      tcaAGCAATGAAACA‑CGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTaca                                                                                                                            <  6:36081/139‑1 (MQ=255)

                      tcaAGCAATGAAACA‑CGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTaca                                                                                                                            <  1:125680/139‑1 (MQ=255)

                      tcaAGCAATGAAACA‑CGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTaca                                                                                                                            <  5:318962/139‑1 (MQ=255)

                      tcaAGCAATGAAACA‑CGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTaca                                                                                                                            <  4:181426/139‑1 (MQ=255)

                      tcaAGCAATGAAACA‑CGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTaca                                                                                                                            <  1:54804/139‑1 (MQ=255)

                       taaccAATGAAACACCGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTaca                                                                                                                            <  1:366083/135‑1 (MQ=255)

                        aaGCAATGCAACA‑CGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACg                                                                                                                          <  6:61488/139‑1 (MQ=255)

                        aaGCAATGAAACA‑CGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACg                                                                                                                          <  2:70330/139‑1 (MQ=255)

                        aaGCAATGAAACA‑CGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACg                                                                                                                          <  6:326172/139‑1 (MQ=255)

                        aaGCAATGAAACA‑CGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACg                                                                                                                          <  7:275339/139‑1 (MQ=255)

                        aaGCAATGAAACA‑CGCCAAACTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACg                                                                                                                          <  3:105103/139‑1 (MQ=255)

                               gAAACA‑CGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑ttttt                                                                                                                                              <  4:367306/112‑1 (MQ=255)

                                 aaCA‑CGACAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATTAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTAC‑ttat                                                                                                                 <  2:37294/138‑4 (MQ=255)

                                       gCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCGTTTTTTAATTTTGTAATTTTAAACTTTAA‑TAAAGGaaa                                                                                                            >  7:22092/1‑139 (MQ=255)

                                       gCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑GTTTTTTAATTTTGAAAGTTTCTCGTTTC‑TATAGGGAAt                                                                                                           >  4:218122/1‑139 (MQ=255)

                                               aaaCAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTAC‑TAAAGGGAATGGAGATa                                                                                                    <  6:108612/138‑1 (MQ=255)

                                               aaaCAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTAC‑TAAAGGGAATGGAGATa                                                                                                    <  7:168206/138‑1 (MQ=255)

                                                aagaaTTTAAGTACCATTACTTAAGAGCAAGTATTGTCTAATTTTAATAGTTATCTATTATTTAACGGGAGGAAATAAATCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTAC‑TAAAGGGAATGGAGATaaa                                                                                                  <  2:340734/136‑1 (MQ=255)

                                                 aCAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTTAATTTTTTAAATTACACGTTTC‑TAAATGGAAATGAGATAAat                                                                                                 >  6:227868/1‑139 (MQ=255)

                                                 aCAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGa                                                                                                                                    <  8:112485/105‑1 (MQ=255)

                                                  cAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTAC‑TAAAGGGAATGGAGATAAatt                                                                                                <  8:196563/139‑1 (MQ=255)

                                                     tttAAGTACCATTTCTTATGATCAAGTATTGTCTATTTTTAATAGTTATCTATTATATAACGGGAGGAAATAATTCTATGCGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTAC‑TAAAGGGAAGGGAGATAAattatt                                                                                             <  3:39679/139‑1 (MQ=255)

                                                     tttAAGTACCATTACTTATGATCAAGTATTGTATATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGGCGC‑TTTTTTAAATTTGGAAAGTTACACGTTAC‑TAAAGGGAATGGAGATAAattatt                                                                                             <  8:289623/139‑1 (MQ=255)

                                                     tttAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTAC‑TAAAGGGAATGGAGATAAattatt                                                                                             <  1:155515/139‑1 (MQ=255)

                                                     tttAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTAC‑TAAAGGGAATGGAGATAAattatt                                                                                             >  8:226881/1‑139 (MQ=255)

                                                     tttAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTAC‑TAAAGGGAATGGAGATAAattatt                                                                                             <  6:393759/139‑1 (MQ=255)

                                                     tttAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTAC‑TAAAGGGAATGGAGATAAattatt                                                                                             <  5:289065/139‑1 (MQ=255)

                                                     tttAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTAC‑TAAAGGGAATGGAGATAAattatt                                                                                             <  3:27747/139‑1 (MQ=255)

                                                     tttAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTAC‑TAAAGGGAATGGAGATAAattatt                                                                                             <  1:80892/139‑1 (MQ=255)

                                                     tttAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTAC‑TAAAGGGAATGGAGATAAattatt                                                                                             <  1:275192/139‑1 (MQ=255)

                                                     tttAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTAC‑TAAAGGGAATGGAGATAAattatt                                                                                             <  7:407542/139‑1 (MQ=255)

                                                     tttAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTAC‑TAAAGGGAATGGAGATAAattatt                                                                                             <  1:220943/139‑1 (MQ=255)

                                                       tAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTAC‑TAAAGGGAATGGAGATAAAttatta                                                                                            <  5:399735/138‑1 (MQ=255)

                                                        aaGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTAC‑TAAAGGGAATGGAGATAAATTATTAg                                                                                           <  6:195093/138‑1 (MQ=255)

                                                        aaGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTAC‑TAAAGGGAATGGAGATAAATTATTAg                                                                                           <  4:62885/138‑1 (MQ=255)

                                                          gTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTAC‑TAAAGGGAATGGAGATAAATTATTAGata                                                                                        <  5:177664/139‑1 (MQ=255)

                                                          gTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTAC‑TAAAGGGAATGGAGATAAATTATTAGata                                                                                        <  1:396771/139‑1 (MQ=255)

                                                             ccATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTAC‑TAAAGGGAATGGAGATAAATTATTAGAtata                                                                                      <  7:298789/138‑1 (MQ=255)

                                                             ccATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑GTTTTTTAATTTTTTAATTTTAAAGTTAC‑TAAAGGGGATGGATATAAATTATtatatata                                                                                      >  5:149696/1‑138 (MQ=255)

                                                               aTTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑GTTTTTTAATTTTTTTATTTTAACGTTACTTAAAGGGAA‑GGATATTAAATATTatatattat                                                                                    >  5:120084/1‑135 (MQ=255)

                                                                ttACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑GTTTTTTAATTTTGTAAGTTTAACGTTAA‑TAAATGGAATTGATCTAAATTATTATTtataaaa                                                                                   >  4:121411/1‑135 (MQ=255)

                                                                      aTGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTAC‑TAAAGGGAATGGAGATAAATTATTAGATATACTACTGAc                                                                              <  3:249367/137‑1 (MQ=255)

                                                                      aTGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTAC‑TAAAGGGAATGGAGATAAATTATTAGATATACTACTGAc                                                                              <  4:251487/137‑1 (MQ=255)

                                                                           cAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑GTTTTTTATTTTTTTAAGTTTAAAGTTTA‑TAAATGGAATTGTTATATATTATTTTTTTTAATAATGACAGCttg                                                                        >  3:92536/1‑137 (MQ=255)

                                                                            aaGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTAC‑TAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCa                                                                      <  1:100154/139‑1 (MQ=255)

                                                                            aaGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTAC‑TAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCa                                                                      <  3:220751/139‑1 (MQ=255)

                                                                            aaGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTAC‑TAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCa                                                                      <  5:204233/139‑1 (MQ=255)

                                                                            aaGTATTGTCTATTATTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGGCGC‑TTTTTTAAATTTGGAAAGTTACACGTTAC‑TAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCa                                                                      <  4:93272/139‑1 (MQ=255)

                                                                             aGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑GTTTTTTAATTTTGTAAGTTAAAAGTTAA‑TAAAGGGAATGGATATAAATTTTTTTATATAATAATTAAAGCTTGCa                                                                      >  2:250345/1‑138 (MQ=255)

                                                                              gttttGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATTATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTAC‑TAAAGGGAATGGAGATAAATTATTAGATATACTTCTGACAGCTTCCa                                                                      <  2:181229/134‑1 (MQ=255)

                                                                              gTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTAC‑TAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCaag                                                                    <  6:86985/139‑1 (MQ=255)

                                                                              gTATTGTCTATTTTTAAAAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTAC‑TAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCaag                                                                    <  4:309228/139‑1 (MQ=255)

                                                                              gTATTGTCTATTATTACTAGTTATCTATTAATTAACCTGACGAAATAATTCTATGAGTCGC‑TTTTTTAAAATTGGAAAGTTACACGTTAC‑TAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCaag                                                                    <  2:149037/139‑1 (MQ=255)

                                                                               tATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTAC‑TAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCaaga                                                                   <  3:332772/139‑1 (MQ=255)

                                                                                atattCTATTATTAATACATATCTATTAATTAACGTGATGAAATAATTCTATGAGACGC‑TTTTATAAATTTGGAAAGTTACACGTTAC‑TTAAGGGAATGGATATAAATTATTAGATATACTACTGACAGCTTCCaaga                                                                   <  8:380193/134‑1 (MQ=255)

                                                                                 ttGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTAC‑TAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCaagaag                                                                 <  2:30981/139‑1 (MQ=255)

                                                                                    tCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTTAAATTTTAAATTTAAACGTTTA‑AAAATGGAATTGAGATAAAATTTTATATATACTAAAGAAAGCATCCAAAAAGca                                                               >  6:169735/1‑137 (MQ=255)

                                                                                    tCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑GTTTTTTAATTTTGTGATTTTCGAGGTAA‑TAAAGGGGATGGAGATAAATTTTTAGATATACTACTGGCAGGGTCCaagcaccg                                                               >  8:317250/1‑133 (MQ=255)

                                                                                      tATTTTTAATATTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTAC‑TAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTaaa                                                            <  3:62897/139‑1 (MQ=255)

                                                                                        tttttAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGTAAAGTTACACGTTAC‑TAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTaaa                                                            <  5:56758/137‑1 (MQ=255)

                                                                                         ttttAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑GTTTTTTAATTTTGTAAGTTTAAAGTTAC‑TTAATGGAATTGAGATAAATTATTATATATAATACTGACAGCTTGCAAGAAGCGAAagag                                                         >  7:381696/1‑139 (MQ=255)

                                                                                         ttttAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑GTTTTTTAATTTGGAAAGTTACACGTTAC‑TAAATGGTATGGAGTAAAATTATTATATATAATACTTAAAGCGTAAAAGAAACTaaaa                                                           >  3:305769/1‑136 (MQ=255)

                                                                                          tttAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTAC‑TAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAagag                                                         <  8:137082/138‑1 (MQ=255)

                                                                                           ttAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTAC‑TAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAgg                                                        <  3:365698/138‑1 (MQ=255)

                                                                                                           attAAACGGGACGAAATAATTCTATGAGTCGC‑TTTTTTAAAGATGGCAAGGTACCCGGAAC‑AAACGGGAATGCAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACgg                                        <  4:402968/138‑1 (MQ=255)

                                                                                                            tttAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTAC‑TAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACgggg                                      <  5:83281/139‑1 (MQ=255)

                                                                                                            tttAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTAC‑TAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACggg                                       <  1:308429/138‑1 (MQ=255)

                                                                                                            tttAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTAC‑TAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACggg                                       <  8:258032/138‑1 (MQ=255)

                                                                                                            tttAACGGGAGGAAATAATTCTATGAGTCGC‑GTTTTTTATTTTTGTTATTTTAACGTTAC‑TAAAAGGTTTTGATATTAATTATTTAATATAAAAATTAAACCGTACAAGAAGCAGAATAGTTTCCCAGCGCCAAAgggg                                      >  2:11064/1‑139 (MQ=255)

                                                                                                              tAACGGGAGTAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTAC‑TAAAGGGATTGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGa                                     <  8:167876/138‑1 (MQ=255)

                                                                                                                       gaAATAATTCTATGAGTCGC‑GTTTTTTAATTTTGTAAGTTAAACGGTAA‑TAAATGGAATTGAGATAAAATATTATATATAATACTGACAACTTACAAGAAACATAAAATGTCCCTAGCGCCTACGGGGAATTTgggggt                           >  2:215383/1‑134 (MQ=255)

                                                                                                                           taatTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTAC‑TAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacatt                       <  2:278992‑M1/139‑10 (MQ=255)

                                                                                                                                   tGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTAC‑TAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg               <  5:348586‑M1/139‑18 (MQ=255)

                                                                                                                                   tGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTAC‑TAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg               <  7:253261‑M1/139‑18 (MQ=255)

                                                                                                                                   tGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTAC‑TAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg               <  3:190258‑M1/139‑18 (MQ=255)

                                                                                                                                   tGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTAC‑TAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg               <  7:113227‑M1/139‑18 (MQ=255)

                                                                                                                                   tGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTAC‑TAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg               <  8:344088‑M1/139‑18 (MQ=255)

                                                                                                                                   tGAGTATC‑TTTTTTAAATTTGGAAAGTTACACGTTAC‑TAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaa                  <  5:262617‑M1/136‑15 (MQ=255)

                                                                                                                                     aGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTAC‑TAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaa             <  7:192807‑M1/139‑20 (MQ=255)

                                                                                                                                     aGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTAC‑TAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaa             <  8:408020‑M1/139‑20 (MQ=255)

                                                                                                                                      gTCGC‑TTTTTTAAATTTGTAAAGTTACACGTTAC‑TAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaa             <  5:227433‑M1/138‑20 (MQ=255)

                                                                                                                                            ttttttAAATTTGGAAAGTTACACGTTAC‑TAAAGGGAATGGAGATAAATTATTACATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGCCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtat       <  3:131859‑M1/139‑26 (MQ=255)

TGTTTACTAAAAATCAGTTTCATCAAGCAATGAAACA‑CGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTAC‑TAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGTATCGGGGTACATTGAAAAAGGAAGAGTAT  >  pSIJ663_pHT315_araE_xy/3591‑3872

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`