BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	pSIJ663_pHT315_araE_xy	3,735	A→T	9.8%	intergenic (+20/‑136)	eryR → / → ampR	–/–

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	pSIJ663_pHT315_araE_xy	3,735	0	A	T	9.8%	28461.2 / inf	9921	intergenic (+20/‑136)	eryR/ampR	–/–
Reads supporting (aligned to +/- strand): ref base A (489/8350); new base T (1045/31); total (1538/8383)
Fisher's exact test for biased strand distribution p-value = 0.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 10125 total aligned reads displayed.

CTAAAAATCAGTTTCATCAAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGG‑AAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGTATCGGGGTACATTGAAAAAGGAAGAGTATGA  >  pSIJ663_pHT315_araE_xy/3597‑3874

      aTCAGTTTCATCAAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTg                                                                                                                                        <  6:222251/139‑1 (MQ=255)

      aTCAGTTTCATCAAGCAATGAAACACGCCAAACTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTg                                                                                                                                        <  6:288839/139‑1 (MQ=255)

       tCAGTTTCATCAAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTgg                                                                                                                                       <  8:103935/139‑1 (MQ=255)

       tCAGTTTCATCAAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTgg                                                                                                                                       <  5:395491/139‑1 (MQ=255)

       tCAGTTTCATCAAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTgg                                                                                                                                       <  1:310115/139‑1 (MQ=255)

        cAGTTTCATCAAGCAATCAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGG‑a                                                                                                                                     <  4:246721/139‑1 (MQ=255)

         aGTTTCATCAAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGG‑a                                                                                                                                     <  5:149674/138‑1 (MQ=255)

                tcaCGCAAAGAAACACGCCAAAGTAAACAATATAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTCATAGTTATCTATTATTTAACGGGATGAAATAATTCTATGAGTCGCTTTTTTAAATTTGG‑AAAGTTaca                                                                                                                             <  5:257783/139‑1 (MQ=255)

                tcaAGCAATGAAACACGCCATAGTAAACAATTTAAGTACCATTACTTCTGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATATAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGG‑AAAGTTaca                                                                                                                             <  8:69128/139‑1 (MQ=255)

                tcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGG‑AAAGTTaca                                                                                                                             <  1:274674/139‑1 (MQ=255)

                tcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGG‑AAAGTTaca                                                                                                                             <  4:273946/139‑1 (MQ=255)

                tcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGG‑AAAGTTaca                                                                                                                             <  4:2068/139‑1 (MQ=255)

                tcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGG‑AAAGTTaca                                                                                                                             <  3:358009/139‑1 (MQ=255)

                tcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGG‑AAAGTTaca                                                                                                                             <  2:147615/139‑1 (MQ=255)

                tcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGG‑AAAGTTaca                                                                                                                             <  7:110709/139‑1 (MQ=255)

                acaAGCAATCAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGG‑AAAGTTaca                                                                                                                             <  1:366784/138‑1 (MQ=255)

                  aatcAATCAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGG‑AAAGTTACACg                                                                                                                           <  3:15926/136‑1 (MQ=255)

                  aaccAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGG‑AAAGTTACACg                                                                                                                           <  8:407704/136‑1 (MQ=255)

                  aaGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGG‑AAAGTTACACg                                                                                                                           <  3:298517/139‑1 (MQ=255)

                  aaGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGG‑AAAGTTACACg                                                                                                                           <  2:70330/139‑1 (MQ=255)

                  aaGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGG‑AAAGTTACACg                                                                                                                           <  3:131593/139‑1 (MQ=255)

                     aaaTGAAACAAGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGTGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGG‑AAAGTTACACGTTa                                                                                                                        <  3:219290/138‑1 (MQ=255)

                       aTGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGG‑AAAGTTACACGTTACt                                                                                                                      <  5:226949/139‑1 (MQ=255)

                          aattacGCCAAAGTTTTCTATTTAAGTAAAATTACTTATGAGCAAGTATTTTTTATTTTTAATAGTTATCTATTATTTAAGGGGAGGATATAATTCTATGAGTCGCTTTTTTAAATTTGT‑AAATTTACACGTTACTaaa                                                                                                                   <  6:53868/135‑1 (MQ=255)

                              acGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCGTTTTTTAATTTTG‑ATAGTTACACGGTTCTAAAagg                                                                                                                >  8:132812/1‑138 (MQ=255)

                              acGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCGGTTTTAAATTTTT‑TAATTTTAACGTTAATaaaatg                                                                                                                >  4:110105/1‑135 (MQ=255)

                               cGCCAAAGTAAACAATTTAAGTACCATTACTTATGATGAAGTATTGTCTATTTTTAATAGTTATCTATTATTTTACGGGTGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGG‑ATAGTTACTCGTTACTAAAGGGaa                                                                                                              <  3:52108/139‑1 (MQ=255)

                               cGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTTG‑AAATTTTAACGTTAGTTAATGGat                                                                                                              >  8:342120/1‑138 (MQ=255)

                               cGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGG‑AAAGTTACACGTTACTAAAGGGaa                                                                                                              <  2:372973/139‑1 (MQ=255)

                               cGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGG‑AAAGTTACACGTTACTAAAGGGaa                                                                                                              <  2:214724/139‑1 (MQ=255)

                                gcaaaaGTAAACAATTTAAGTACCATTACTTATGAGCAAGAATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTTAAATTTT‑TAAGTTAAACGATAATAAATAGAAt                                                                                                             >  6:389751/4‑139 (MQ=255)

                                gCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTTAATTTTG‑TAAGTTTAACGTTACTaaatctttg                                                                                                             >  2:44333/1‑133 (MQ=255)

                                gCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGG‑AAAGTTACACGTTACTAAAGGGAAt                                                                                                             >  7:213291/1‑139 (MQ=255)

                                 ccAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGG‑AAAGTTACACGTTACTAAAGGGAATg                                                                                                            <  4:364743/139‑1 (MQ=255)

                                    aaGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGG‑AAAGTTACACGTTACTAAAGGGAATGgag                                                                                                         <  2:180044/139‑1 (MQ=255)

                                       tAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGG‑AAAGTTACACGTTACTAAAGGGAATGGAGATa                                                                                                      <  8:284981/139‑1 (MQ=255)

                                         taCAATTTAAGTTCCATTACTTATGAGCTAGTTTTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGTAAATAAATCTATGAGTCGCTTTTTTAAATTTGG‑AAAGTTACACGTTAGTTTAGGGAATGGAGATaaa                                                                                                    <  1:154089/138‑1 (MQ=255)

                                         aaCAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGG‑AAAGTTACACGTTACTAAAGGGAATGGAGATaaa                                                                                                    <  4:302694/139‑1 (MQ=255)

                                         aaCAATTTAAGAACCATTACTTATGAGCAAGGATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGG‑AAAGTTACACGTTACTAAAGGGAATGGAGATaaa                                                                                                    <  8:192478/139‑1 (MQ=255)

                                          aCAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGG‑AAAGTTACACGTTACTAAAGGGAATGGAGATAAat                                                                                                   <  7:243289/139‑1 (MQ=255)

                                            aaTTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGG‑AAAGTTACACGTTACTAAAGGGAATGGAGATAAatta                                                                                                 <  8:222658/139‑1 (MQ=255)

                                             atatAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGG‑AAAGTTACACGTTACTAAAGGGAATGGAGATAAattat                                                                                                <  5:7657/136‑1 (MQ=255)

                                              tttAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGG‑AAAGTTACACGTTACTAAAGGGAATGGAGATAAattatt                                                                                               <  4:169942/139‑1 (MQ=255)

                                              tttAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGG‑AAAGTTACACGTTACTAAAGGGAATGGAGATAAattatt                                                                                               <  1:333836/139‑1 (MQ=255)

                                              tttAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGG‑AAAGTTACACGTTACTAAAGGGAATGGAGATAAattatt                                                                                               <  3:390096/139‑1 (MQ=255)

                                              tttAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGG‑AAAGTTACACGTTACTAAAGGGAATGGAGATAAattatt                                                                                               <  4:60894/139‑1 (MQ=255)

                                              tttAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGG‑AAAGTTACACGTTACTAAAGGGAATGGAGATAAattatt                                                                                               <  5:203720/139‑1 (MQ=255)

                                                 aaGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGTGTCGCTTTTTTAAATTTGG‑AAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAg                                                                                             <  4:92140/138‑1 (MQ=255)

                                                 aaGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGG‑AAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAg                                                                                             <  8:379108/138‑1 (MQ=255)

                                                 aaGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGG‑AAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAg                                                                                             <  6:16220/138‑1 (MQ=255)

                                                 aaGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGG‑AAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGa                                                                                            <  1:78723/139‑1 (MQ=255)

                                                 aaGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGGCGCGTTTTTTAATTTGGTAAAATAGCACGTT‑CAAAATCGTATGGAGATTAAttattat                                                                                             >  7:81939/1‑137 (MQ=255)

                                                  aGTACCATTTCTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGG‑AAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGat                                                                                           <  3:272370/139‑1 (MQ=255)

                                                  aGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGG‑AAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGat                                                                                           <  5:49538/139‑1 (MQ=255)

                                                  aGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGG‑AAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGat                                                                                           <  5:316214/139‑1 (MQ=255)

                                                   gTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGG‑AAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGata                                                                                          <  4:401277/139‑1 (MQ=255)

                                                   gTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGG‑AAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGata                                                                                          <  7:269593/139‑1 (MQ=255)

                                                      ccATTACTTATGTGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTTACTGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGG‑AAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGAtata                                                                                        <  2:248492/138‑1 (MQ=255)

                                                         ttACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCGTTTTTAAATTTTG‑AAAGTTTAACGTTACTAAATGGAATTGTTATAAATTATTATATAtactac                                                                                    >  7:189300/1‑139 (MQ=255)

                                                            cTTATGAGCAAGTATTGTCTAATTTTAATAGTTATCTATTATTTAACGGGAGGAAAAAATTCTAAGAGTCGCGTTTTTAAATTTGG‑AAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGa                                                                                 <  2:308115/139‑1 (MQ=255)

                                                             atATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGG‑AAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGAc                                                                                <  2:339653/138‑1 (MQ=255)

                                                               aTGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGG‑AAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTATATATACTACTGAc                                                                                <  6:12942/137‑1 (MQ=255)

                                                                     aaGTATTGTCTATTTTTACTAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGG‑AAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCa                                                                        <  5:118696/139‑1 (MQ=255)

                                                                     aaGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGG‑AAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCa                                                                        <  5:370984/139‑1 (MQ=255)

                                                                     aaGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGG‑AAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCa                                                                        <  7:57787/139‑1 (MQ=255)

                                                                     aaGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGG‑AAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCa                                                                        <  8:31494/139‑1 (MQ=255)

                                                                     aaGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGG‑AAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCa                                                                        <  1:100154/139‑1 (MQ=255)

                                                                       gTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGG‑AAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCaag                                                                      <  3:325923/139‑1 (MQ=255)

                                                                       gTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGG‑AAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCaag                                                                      <  6:189577/139‑1 (MQ=255)

                                                                         aTTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGG‑AAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCaaga                                                                     <  6:257969/138‑1 (MQ=255)

                                                                          ttGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGG‑AAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCaagaag                                                                   <  5:99846/139‑1 (MQ=255)

                                                                                  ttttAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCGTTTTTTAATTTTG‑TAAGTTTAACGTTTCTAAATGGAATTGAGATAAATTATTTTATTTACTACTGACAACTTGCAATAAGCAAAagag                                                           >  4:334993/1‑139 (MQ=255)

                                                                                   tttAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGG‑AAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAagag                                                           <  7:162982/138‑1 (MQ=255)

                                                                                    ttAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGG‑AAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAgg                                                          <  3:82336/138‑1 (MQ=255)

                                                                                       aTAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTCTAAATTTGG‑AAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTccc                                                      <  3:104321/139‑1 (MQ=255)

                                                                                           ttATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTTG‑ATATTTAAACGTTTAAAAAAGGAATTGAGAATAATTATTATATATAATACTGACAGGATCCAAAAACCAAAATAGGTGCCAAga                                                  >  2:109139/1‑138 (MQ=255)

                                                                                           ttATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCGTTTTTTAATTTTG‑AAAGTTAAACGTTTCAAAAAGGTATTGGGATAAAATATTATATTAAATAATGACAACTTACAAGAATATAAAGAGGTACCAAgc                                                  >  4:138641/1‑139 (MQ=255)

                                                                                             atatattatTTAACGTGAGGAATAAATTATATGAGTCGCTTTTTTAAATTTGG‑AAAGTTACACGTTACTAATGGGAATGGATATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAgcg                                                 <  2:277074/135‑1 (MQ=255)

                                                                                                tattatTTAACGGGAGGAAATAATTCTATGAGTCGCGTTTTTAAATTTGG‑AAAGTTTCACGGTACTTAATGGTATGGAGTTTTTTTTTTAGTTTTAGGTCTGACAGCTTACAAGAAGCTAAAGAGGTCACGAgcgcgg                                              >  6:83839/1‑136 (MQ=255)

                                                                                                  ttattTAACGGGAGGAAATAATTCTATGAGTCGCTTTTGTAAATTTGG‑AAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGcc                                               <  7:318036/135‑1 (MQ=255)

                                                                                                    attTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGG‑AAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACgg                                          <  1:220890/138‑1 (MQ=255)

                                                                                                     tttAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGG‑AAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACgggg                                        <  5:344503/139‑1 (MQ=255)

                                                                                                     tttAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGG‑AAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACggg                                         <  1:395292/138‑1 (MQ=255)

                                                                                                       tAACGGGAGGAAATAATTCTATGAGTCGCGTTTTTTTATTTTG‑TAATTTTAACGTTTATAAATGGAATGGAGATAAATTTTTATATATAATAATGACAGCTTCCAACAATCTAAAGATGTCCCACTCGCCGACGGGGaa                                      >  6:321239/1‑139 (MQ=255)

                                                                                                          cGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTTG‑AAAGTTACACGTTTCTAAATGGAATGGATTTAAATTATTATATAAACTACTGACAGCTTGCAAGAAGCTAAAGAGGTACCTAGCGCCGACGGGGTAttt                                   >  5:175374/1‑139 (MQ=255)

                                                                                                                 aaaTAATTCTATGAGTCGCTTTTTTAAATTTGG‑AAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtac                            <  3:187870‑M1/139‑7 (MQ=255)

                                                                                                                  aataatTCTATGAGTCGCTTTTTTAAATTTGG‑AAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtaca                           <  7:13245‑M1/139‑8 (MQ=255)

                                                                                                                  aataatTCAATTAGTCGCTTTTTTAAATTTGG‑AAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtac                            <  1:189673‑M1/138‑7 (MQ=255)

                                                                                                                    taatTCTATGAGTCGCTTTTTTAAATTTGG‑AAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacatt                         <  7:295125‑M1/139‑10 (MQ=255)

                                                                                                                    taatTCTATGAGTCGCTTTTTTAAATTTGG‑AAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacatt                         <  6:354278‑M1/139‑10 (MQ=255)

                                                                                                                            tGAGTCGCTTTTTTAAATTTTG‑AAAGTTACAAGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                 <  1:250295‑M1/139‑18 (MQ=255)

                                                                                                                            tGAGTCGCTTTTTTAAATTTGG‑AAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                 <  7:372494‑M1/139‑18 (MQ=255)

                                                                                                                            tGAGTCGCTTTTTTAAATTTGG‑AAAGTTACACGTTACTAAAGGGAAGGTAGATAAATTCTAAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGGCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                 <  1:125492‑M1/139‑18 (MQ=255)

                                                                                                                             tagTCGCTTTTTTAAATTTTG‑AAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTATATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                 <  5:285926‑M1/137‑18 (MQ=255)

                                                                                                                             gAGTCTCTTTTTTAAATTTTT‑AAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                 <  3:245170‑M1/138‑18 (MQ=255)

                                                                                                                              aGTCGCTTTTTTAAATTTGG‑AAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaa               <  8:25454‑M1/139‑20 (MQ=255)

                                                                                                                              aGTCGCTTTTTTAAATTTGG‑AAAGTTACAAGTAACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaa               <  8:164158‑M1/139‑20 (MQ=255)

                                                                                                                               gTCGCTTTTTTAAATTTGG‑AAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTATATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaag              <  7:342120‑M1/139‑21 (MQ=255)

                                                                                                                                    ttttttAAATTTGG‑AAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtat         <  7:403109‑M1/139‑26 (MQ=255)

                                                                                                                                          aaaTTTGG‑AAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagta    <  1:53075‑M1/138‑31 (MQ=255)

CTAAAAATCAGTTTCATCAAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGG‑AAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGTATCGGGGTACATTGAAAAAGGAAGAGTATGA  >  pSIJ663_pHT315_araE_xy/3597‑3874

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`