BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	pSIJ663_pHT315_araE_xy	5,467	T→G	5.9%	intergenic (+736/–)	ampR → / –	–/–

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	pSIJ663_pHT315_araE_xy	5,467	0	T	G	5.9%	26141.4 / 301.3	12049	intergenic (+736/–)	ampR/–	–/–
Reads supporting (aligned to +/- strand): ref base T (5246/5835); new base G (364/565); total (5624/6425)
Fisher's exact test for biased strand distribution p-value = 1.60e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 12141 total aligned reads displayed.

GCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAG  >  pSIJ663_pHT315_araE_xy/5329‑5604

gCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGt                                                                                                                                           <  5:72103/139‑1 (MQ=255)

    aCAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAgg                                                                                                                                       <  8:351084/139‑1 (MQ=255)

    aCAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAgg                                                                                                                                       <  8:163050/139‑1 (MQ=255)

           tCCGGTATGCGGCAGGGGCGGAACAGGAGAGCGCACGATGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTGCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCg                                                                                                                                 <  8:217584/138‑1 (MQ=255)

           tCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCg                                                                                                                                 <  2:90178/138‑1 (MQ=255)

             cGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGa                                                                                                                               <  2:352963/138‑1 (MQ=255)

                  aGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTa                                                                                                                          >  4:105421/1‑138 (MQ=255)

                   gCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATg                                                                                                                        >  8:384959/1‑139 (MQ=255)

                       cAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAgg                                                                                                                                       <  7:176001/120‑1 (MQ=255)

                          gttCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTATGCCTTGAGCGTCGATTTTTGGGAGGCTCGGCAGGGGGGCGGAGCCTAAGGAAAAAc                                                                                                                 >  4:316657/3‑139 (MQ=255)

                                    ggAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAAcg                                                                                                       <  3:285104/139‑1 (MQ=255)

                                    ggAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTAGATTTTTGTGGTGCTCGTCCGGGGGGCGGAGCCTATTGAAAAACGCCAGCAAcg                                                                                                       >  1:133691/1‑139 (MQ=255)

                                     gagaGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAAcgc                                                                                                      >  7:393815/1‑139 (MQ=255)

                                       gcgcgcACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGGCGGGTGTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGGCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCgg                                                                                                    <  3:194157/137‑1 (MQ=255)

                                       gagCGCACGTGGGAGCTTCCAGGGGGAAACGCATGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTTACTTGAGGGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCgg                                                                                                    <  7:305631/139‑1 (MQ=255)

                                         gcgcACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCg                                                                                                                                 <  6:403541/108‑1 (MQ=255)

                                         gcgcACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGAGTTTTTTTATGGTCGTCAGGGGGGCGGAGCCGATGGGAAAACCCCCGCCACGCgggc                                                                                                  >  5:32181/1‑137 (MQ=255)

                                          cgcACGAGGGAGCTTCCAGGGGGAACCGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGCCTTGAGCGTCGAGTTTTGTGAGGCTGATCAGGGGGGGGGCGCCTTTGGCAAAACCCCAGCAACCCGGcga                                                                                                 >  2:137937/1‑137 (MQ=255)

                                          cgcACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTTACTTGAGCGTCGATTTTTGTGATTATCGTCAGGGGGGCGGAGCCTATGGTAAAACGCCAGCAACGCGGCCt                                                                                                 >  3:312871/1‑139 (MQ=255)

                                          cgcACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGCCTTGAGCGTCGATGTTTGTGATGCTGGTCAGGGGGGCGGCGCCTATGGAAAAACGCCCGCAACGCGGCCt                                                                                                 >  7:228520/1‑139 (MQ=255)

                                          cgcACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTGAGGGGGGCGGAGCCGTTGGAAAAACGCCAGCCACGCGGccg                                                                                                 >  6:129140/1‑138 (MQ=255)

                                          cgcACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGccg                                                                                                 >  7:101599/1‑138 (MQ=255)

                                          cgcACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGccg                                                                                                 >  7:125526/1‑138 (MQ=255)

                                          cgcACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCt                                                                                                 >  6:376340/1‑139 (MQ=255)

                                          cgcACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGAGTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCCACGCGGCCt                                                                                                 >  8:13279/1‑139 (MQ=255)

                                             aCGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCtttt                                                                                              >  6:187439/1‑139 (MQ=255)

                                               gAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCCGCAACGCGGCCTTTTTa                                                                                            >  8:323355/1‑139 (MQ=255)

                                               gAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTa                                                                                            <  2:60174/139‑1 (MQ=255)

                                               gAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGAGTTTTGTGATGCTCGGCAGGGGGGCGGAGCCTATGGAAAAACCCCCGCAACGCGGCCTTGTTa                                                                                            >  4:254556/1‑139 (MQ=255)

                                                aGGGAGCTTCCTGGGGTAAACGCCTGGTATCTTTAAAGTCCTGTCGGGTTTCGCCACCTCTTACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTAc                                                                                           <  1:290524/139‑1 (MQ=255)

                                                       ttCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGGTCGTCAGGGGGGCGGCGCCTATGGAAAAACCCCACCCACGCGGCCGTTTTACGGTTCct                                                                                    >  6:245602/1‑139 (MQ=255)

                                                           cgggggAAACGCCTGGTATCTTTATAGTCCGGTGGGGTTTCGCCACCGCTGACTTGAGCGTCGATTTTTGGGATGCGCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCctggcc                                                                                <  5:260234/138‑1 (MQ=255)

                                                           aGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCctggcc                                                                                <  1:52332/139‑1 (MQ=255)

                                                                 aaaCGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTTTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTTCGGTTCctggccttttgc                                                                          >  5:291429/1‑139 (MQ=255)

                                                                 aaaCGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCctggccttttgc                                                                          <  6:209734/139‑1 (MQ=255)

                                                                   aCGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCATATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCctggccttttgctg                                                                        <  3:395288/139‑1 (MQ=255)

                                                                        tGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGGCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCctggccttttgctggcctt                                                                   <  5:226290/139‑1 (MQ=255)

                                                                          gTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCctggccttttgctggcctt                                                                   <  1:160736/137‑1 (MQ=255)

                                                                             gcTTTATCGGCCTGTGGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGGCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTggccttttgctggccttttgct                                                              <  3:344525/138‑1 (MQ=255)

                                                                              ggttATAGTGATGGGGGGTTTCGCCACCGCTGAGTTGAGCGTCGATTTTGGGTCTGGTCGTCTGGGTGGCGGTGACTATGGAAACTCGGCAGCTAAGCGGCCTTTTTACGGTGCctttccttttgctggcctttttgt                                                              <  4:162853/136‑4 (MQ=255)

                                                                              cTTTATCGTCCTGGCGGGTTTCGCCACCTCTGACTTGCGCGTCGATTTTTGTGATGCTCGTCTGGGGGGCGGAGCCTAGGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTggccttttgctggccttttgct                                                              <  1:196597/138‑1 (MQ=255)

                                                                               tttATAGTCCTGTCGGGTTTCGCAACCTCTTAATTTAGCGTCGATTTTTGTTATTCAAGTCAGTGGGTACGAACCAAAGTAAAAACCCCAACAACCCGGCCGTTTTAAGGAAAcaggcctttttcaggccgtttgata                                                             >  4:55185/1‑135 (MQ=255)

                                                                                ttATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGGGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTcc                                                                                     <  5:120314/113‑1 (MQ=255)

                                                                                 tatcggccTGGCGGGGTTCGCCACCTCTGACTTGCGCGGCGATTTTTGTGATGCGCGGCCGGGGGGCGGAGCCTATGTAAACACGCCAGCAACGCGGCCTTTTTACGGTTCCTggccttttgctggccttttgct                                                              <  3:222004/129‑1 (MQ=255)

                                                                                 tataGTCCTGTCGGGTGTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTggccttttgctggccttttgct                                                              <  8:46093/135‑1 (MQ=255)

                                                                                 tataGCCCTGTCGGGTTTCGCCACATCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAACACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCAC                                                           <  5:149215‑M1/138‑1 (MQ=255)

                                                                                        cTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTc                                                             <  5:351628/129‑1 (MQ=255)

                                                                                        cTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTgcgg                                                   <  2:220920‑M1/139‑5 (MQ=255)

                                                                                        cTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTgcgg                                                   >  1:74330‑M1/1‑135 (MQ=255)

                                                                                        cTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTgcgg                                                   >  4:401733‑M1/1‑135 (MQ=255)

                                                                                        cTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTAGTCAGGGGGGCGGAGCCTATGGAAAAACGCCCCCCACGCGGCCGTTTTACGGTTCCTGGCCGTTTGCTGGGCTTTTGGTCCCATGTgcgg                                                   >  6:282090‑M1/1‑135 (MQ=255)

                                                                                        cTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCGCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTgcgg                                                   <  5:409257‑M1/139‑5 (MQ=255)

                                                                                        cTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCGCGTAAGGGGGGCGGAGGCAATGGAAAAACGCCCGCAACGCCGCCGTTTTACGGTTCATGGCCTTTTGCTGGCAGTGTTCTAACATGTgcgg                                                   >  2:287268‑M1/1‑135 (MQ=255)

                                                                                         tGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTTTGATGGGAGTCAGGGGGGCGGAGCCTATTGAAAAACGCCCGCACCGCCGCCATTTTACGGTTCCTGGGCGTTTGCGGGGCGTTTGCTCACATGtgcggc                                                  >  4:286575/1‑134 (MQ=255)

                                                                                         tGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTa                                                                                                                          >  5:379761/1‑67 (MQ=255)

                                                                                         tGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTa                                                                                            <  7:73689/97‑1 (MQ=255)

                                                                                         tGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTggccttttgctggccttttgct                                                              >  7:334571/1‑127 (MQ=255)

                                                                                           tCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGGTAGTCATGGGGGCGGAGCCAATGGAAAAACGCCAGCAACCCGGCCTTTTTACGGTTACTGGCCTTTTTCTGGCCTTTTGGTCACATGTgcggcgg                                                >  8:24360‑M1/1‑132 (MQ=255)

                                                                                           tCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTgcggcgg                                                >  1:258934‑M1/1‑132 (MQ=255)

                                                                                           tCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTgcggcgg                                                >  7:253776‑M1/1‑132 (MQ=255)

                                                                                           tCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTgcggcgg                                                >  6:157480‑M1/1‑132 (MQ=255)

                                                                                            cGGGTTTCGCCACCTCTGATGTGTGCGGCGATTTTTGTGAGGCTCGGCTGGGGGGGGGAGCCTATGTAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTgcggcggg                                               <  4:215004‑M1/139‑9 (MQ=255)

                                                                                                tttCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGTGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTTCGCGCCTTTTGCAGACATGTtcggcggggcat                                           >  6:72519‑M1/1‑127 (MQ=255)

                                                                                                  tCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTgcggcggggcttgg                                         >  2:17342‑M1/1‑125 (MQ=255)

                                                                                                  tCGCCACCTCTGACGTGAGCGGCGATTTTTGTGTTGCTCGTCTGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTgcggcggggcttg                                          <  8:270018‑M1/138‑14 (MQ=255)

                                                                                                  gcgcgaCCTCTGAATTGAGCGTCGATTTTTGTGATGCTCGTCTGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACTCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTgcggcggggcttg                                          <  7:36062‑M1/137‑14 (MQ=255)

                                                                                                   cGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTgcggcggggcttgg                                         <  6:348551‑M1/138‑15 (MQ=255)

                                                                                                    gCCACCTCTGACTTTCGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTgcggcggggcttggcg                                       <  4:348872‑M1/139‑17 (MQ=255)

                                                                                                     ccACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTgcggcggggcttggcgg                                      <  3:371594‑M1/139‑18 (MQ=255)

                                                                                                      cACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTgcggcggggcttggcggc                                     <  1:231526‑M1/139‑19 (MQ=255)

                                                                                                      cACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTgcggcggggcttggcggc                                     <  2:318569‑M1/139‑19 (MQ=255)

                                                                                                      cACCTCTGACTTGAGCGTCGATTTTTGTGATGCGCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTgcggcggggcttggcggc                                     <  1:100580‑M1/139‑19 (MQ=255)

                                                                                                      cACCTCTGACTTGAGCGTCGATTTTTGTGATGCGCGGCTGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTgcggcggggcttggcggc                                     <  1:352936‑M1/139‑19 (MQ=255)

                                                                                                       aCCTCTTACTTTAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTTTGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTgcggcggggcttggcggct                                    <  3:76831‑M1/139‑20 (MQ=255)

                                                                                                       aCCTCTGACTTGAGCGTCGATTTTTGTGAGGCTCGGCCGGGGGGCGGAGCCTATGGAAACACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTgcggcggggcttggcggct                                    <  1:386970‑M1/139‑20 (MQ=255)

                                                                                                         ctctGACTTGAGCGTCGATTTTTGTGTTGCTCGGCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTgcggcggggcttggcggctta                                  <  8:188724‑M1/139‑22 (MQ=255)

                                                                                                         ctctGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTgcggcggggcttggcggctta                                  <  8:73325‑M1/139‑22 (MQ=255)

                                                                                                         ctctGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTgcggcggggcttggcggctta                                  <  8:103009‑M1/139‑22 (MQ=255)

                                                                                                         ctctGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTgcggcggggcttggcggctta                                  >  5:173055‑M1/1‑118 (MQ=255)

                                                                                                         ctctGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTgcggcggggcttggcggctta                                  <  4:187343‑M1/139‑22 (MQ=255)

                                                                                                          tctGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCgg                                                                                                    >  5:197889/1‑72 (MQ=255)

                                                                                                            tGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGa                                                                                                                               <  2:256410/43‑1 (MQ=255)

                                                                                                              cctTGAGCGGCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAACACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTgcggcggggcttggcggcttattgta                             <  6:314656‑M1/138‑27 (MQ=255)

                                                                                                              aCTTGAGCTTTGATTTTGGTGTTGATCGTGCGGGGGGGGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTGTTTACGGTTCCTTGCCTTTTGCTGGCCTTTTGCTCACATGTgcggcggggcttggcggcttattgt                              <  7:152236‑M1/138‑26 (MQ=255)

                                                                                                              aCTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTgcggcggggcttggcggcttattgt                              <  2:40013‑M1/138‑26 (MQ=255)

                                                                                                              aCTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTgcggcggggcttggcggcttattgt                              <  1:320841‑M1/138‑26 (MQ=255)

                                                                                                              aCTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTgcggcggggcttggcggcttattgt                              <  7:275392‑M1/138‑26 (MQ=255)

                                                                                                                   aGCGTCGATTTTTTTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTTCGGTTACTGGCCTTTTGCTGGCCCTTTGCTCACATGTgcggcggggcttggcgggtgattttaagga                         >  4:87745‑M1/1‑108 (MQ=255)

                                                                                                                   aGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGaaa                                                                                                                    >  2:103684/1‑47 (MQ=255)

                                                                                                                   aGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTgcggcggggcttggcggcttattgtatggc                         >  7:20126‑M1/1‑108 (MQ=255)

                                                                                                                   aGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTgcggcggggcttggcggcttattgtatggc                         >  3:172600‑M1/1‑108 (MQ=255)

                                                                                                                   aGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCGTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCCCATGTgcggcggggcttggcggcttattgtatggc                         >  8:293353‑M1/1‑108 (MQ=255)

                                                                                                                   aGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCGTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTgcggcggggcttggcggcttattgtatggct                        >  4:136444‑M1/1‑108 (MQ=255)

                                                                                                                       tctatttTTGTGATGCTCGTCCGGGGGGCGGAGCCTATGGAAACACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTgcggcggggcttggcggcttattgtatggctatga                    <  3:254556‑M1/136‑36 (MQ=255)

                                                                                                                        cGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTgcggcggggcttg                                          <  4:374434‑M1/116‑14 (MQ=255)

                                                                                                                         gATTTTTGTGTTGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTgcggcggggcttggcggcttattgtatggctatgaca                  <  7:50436‑M1/139‑38 (MQ=255)

                                                                                                                         gATTTTTGTGATGGTCGGCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTgcgggggggcttggcggcttattgtatggctatgaca                  <  3:121108‑M1/139‑38 (MQ=255)

                                                                                                                         gATTTTTGTGATGCGCGTCCGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTgcggcggggcttggcggcttattgtatggctatgaca                  <  3:14957‑M1/139‑38 (MQ=255)

                                                                                                                               tgtgATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTgcggcggggcttggcggcttattgtatggctatgacacggca             <  5:95202‑M1/138‑43 (MQ=255)

                                                                                                                                         gTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTgcggcggggcttggcggcttattgtatggc                         <  3:417019‑M1/116‑31 (MQ=255)

GCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAG  >  pSIJ663_pHT315_araE_xy/5329‑5604

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`