Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	BS168_reference_genome	390,006	A→C	19.2%	T755P (ACA→CCA)	srfAB →	surfactin synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	BS168_reference_genome	390,006	0	A	C	19.2%	89.5 / 7.0	60	T755P (ACA→CCA)	srfAB	surfactin synthetase
Reads supporting (aligned to +/- strand):  ref base A (17/31);  new base C (10/2);  total (27/33)
Fisher's exact test for biased strand distribution p-value = 3.76e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTTGCTGCAAAGGATGCGGGTATGTTCGGCACACTGCGCCATTTAATCATCGGCGGAGATGCCCTTGTCCCGCATATTGTCAGCAAAGTAAAACAGGCATCGCCGTCGCTTTCATTGTGGAACGGATACGG‑CCCGACAGAAAACACGA‑CGTTTTCAACCAGTTTTCTGATCGACCGCGAGTATGGCGGCTCTATCCCAATCGGGAAGCCGATCGGAAATTCCACTGCCTATATTATGGATGAGCAGCAATGCCTGCAGCCAATCGGCGCGCCTG  >  BS168_reference_genome/389871‑390144                                                                                                                                         |                                                                                                                                            cTTGCTGCAAAGGATGCGGGTATGTTCGGCACACTGCGCCATTTAATCATCGGCGGAGATGCCCTTGTCCCGCATATTGTCAGCAAAGTAAAACAGGCATCGCCGTCGCTTTCATTGTGGAACGGATACGG‑CCCGa                                                                                                                                             >  2:220213/1‑136 (MQ=255)                gCGGGTATGTTCGGCACACTGCGCCATTTAATCATCGGCGGAGATGCCCTTGTCCCGCATATTGTCAGCAAAGTAAAACAGGCATCGCCGTCGCTTTCATTGTGGCACGGCTACGG‑GCCGCCAGCAAACACGA‑CGtttt                                                                                                                          >  3:312254/1‑139 (MQ=255)                    gTATGTTGGTCACACTGCGCCATTTAATCATCGGCGGAGATGCCCTTGGCCCGATTATTGTCAGAAAAGTAAAACAGGCATCGCCGTCGCTTTCTTTGGGGACCGTATACGG‑CCCGACAGAAAACACGA‑CGTTTTCAAc                                                                                                                      <  5:285588/139‑1 (MQ=255)                       tGTTCGGCACACTGCGCCATTTAATCATCGGCGGAGATGCCCTTGTCCCGCATATTGTCAGCAAAGTAAAACAGGCATCGCCGTCGCGTTAATTGTGGAACGGATAAGG‑CCCCCCCGAAAACACGC‑CGGTTTGAACCAg                                                                                                                   >  4:316105/1‑139 (MQ=255)                              cacaCTGCGCCATTTAATCATCGGCGGAGATGCCCTTGTCCCGCATATTGTCAGCAAAGTAAAACAGGCATCGCCGTCGCTTTGATTGTGGTACGGTTAAGG‑CCCCACAGAAAACACCC‑CCTTTTAAACCAGTTTTATg                                                                                                            >  3:13404/1‑139 (MQ=255)                              cacaCTGCGCCATTTAATCATCGGCGGAGATGCCCTTGTCCCGCATATTGTCAGCAAAGTAAAACAGGCATCGCCGTCGCTTTCATTGTGGAACGGATACGG‑CCCGACAGAAAACACGA‑CGTTTTCAACCAGTTTTCt                                                                                                             >  7:268491/1‑138 (MQ=255)                              cacaCTGCGCCATTTAATCATCGGCGGAGATGCCCTTGTCCCGCATATTGTCAGCAAAGTAAAACAGGCATCGCCGTCGCGTTCATTGTGGTACGGGTACGG‑CCCGACAGAAAACACGA‑CGTTTTCAACCAGTTTTCTg                                                                                                            >  3:67270/1‑139 (MQ=255)                              cacaCTGCGCCATTTAATCATCGGCGGAGATGCCCTTGTCACGCATATTGTCAGCAAAGTAAAACAGGCATCGCCGTCGCTTTAATTGTGGAACGGATACGG‑CCCGACAGGAAAAACGA‑AGTGTTGCACCAGttttat                                                                                                             >  4:348307/1‑136 (MQ=255)                                   tGCGCCATTTAATCATCGGCGGAGATGCCCTTGTCCCGCATATTGTCAGCAAAGTAAAACAGGCATCGCCGTCGCTTTCATTGTGGAACGGATACGG‑CCCGACAGAAAACACCACCGTTTTC‑ACCAGTTTTGTGATCGa                                                                                                       >  2:219423/1‑139 (MQ=255)                                   tGCGCCATTTAATCATCGGCGGAGATGCCCTTGTCCCGCATATTGTCAGCAAAGTAAAACAGGCATCGCCGTCGCGTTGATTTTGGGACCGGTACGG‑CCCGCCCGCAAACACGC‑CGGTTTGACCCAGGTTTGTGGTCGa                                                                                                       >  3:269367/1‑139 (MQ=255)                                   tGCGCCATTTAATCATCGGCGGAGATGCCCTTGTCCCGCATATTGTCAGCAAAGTAAAACAGGCATCGCCGTCGCGTTGATTGTGGAACGGATACGG‑CCCGCCACAAAACCCGA‑CGTTTTCAACCAGTTTTCTGATCGa                                                                                                       >  4:150932/1‑139 (MQ=255)                                   tGCGCCATTTAATCATCGGCGGAGATGCCCTTGTCCCGCATATTGTCAGCAAAGTAAAACAGGCATCGCCGTCGCGTTCATTGTGGGAAGGATACGG‑CCCGCCCGCAAACACGA‑CCTTTTGAACCCGTTTTCTGATCgg                                                                                                       >  7:235014/1‑138 (MQ=255)                                      gcaaTTTAACCTGCGGCGGAGATGCCCTTGTCCCGAATATTGTCAGCAAAGAAAACCCGGCATCGCCGTCGCTTTCATTGTGTACCGGATACGG‑CCCGACAGAAAACACGA‑CGTTTTCAACCAGTTTTCTGATCGACcg                                                                                                    <  4:63836/136‑1 (MQ=255)                                          tttAATCATCGGCGGATATGCCCTTGCCCCGAATTTTGTCACCAAAGAAAAACCGGCATCGCCGTCGCTTTCATTGTGGAACGGAAACGG‑CCCGACACAAAACACGA‑CGTTTTCAACCAGTTTTCTGATCGACCGCGAg                                                                                                <  4:67270/139‑1 (MQ=255)                                               tcatcGGCGGAGATGCCCTTGTCCCGCATATTGTCAGCAAAGTAAAACCGCCATCGCCGGCGCTTTCATTGTGGAACGGATACGG‑CCCGACAGAAAACACGA‑CGTTTTCAACCAGTTTTCTGATCGACCGCGAGTATgg                                                                                           <  7:96256/139‑1 (MQ=255)                                               tcatcGGCGGAGATGCCCTTGTCCCGCATATTGTCAGCAAAGTAAAACAGGCATCGCCGTCGCTTTCATTGTGGAACGGATCCGG‑CCCGCCAGAAAACACGA‑CGTTTTCAACCAGTTTTCTGATCGACCGCGAGTATgg                                                                                           <  3:132334/139‑1 (MQ=255)                                                  tcGGCGGAGATGCCCTTGTCCCGCATATTGTCAGCAAAGTAAAACAGGCATCGCCGTCGCTTTCATTGTGGAACGGATACGG‑CCCGACAGAAAACACGA‑CGTTTTCAACCAGTTTTCTGATCGACCGCGAGTATgg                                                                                           <  7:227035/136‑1 (MQ=255)                                                  tcGGCGGAGATGCCCTTGTCCCGCATATTGTCAGCAAAGTAAAACAGGCATCGCCGTCGCTTTCATTGTGGAACGGATACGG‑CCCGACAGAAAACACGA‑CGTTTTCAACCAGTTTTCTGATCGACCGCGAGTATgg                                                                                           >  8:227035/1‑136 (MQ=255)                                                    ggcggAGATGCCCTTGTCCCGCATATTGTCAGCAAAGTAAAACAGGCATCGCCGTCGCTTTCATTGTTGAACGGATACGG‑CCCGACAGAAAACACGA‑CGTTTTGAACCAGTTTTCTGATCGACCGGGCGTATggcggcg                                                                                      >  8:213979/1‑138 (MQ=255)                                                    ggcggAGATGCCCTTGTCCCGCATATTGTCAGCAAAGTAAAACAGGCATCGCCGTCGCGTTGATTGTGGGACGGATACGG‑CCCGCCAGAAAACACCA‑CGGTTTAAACCAGTTTTGTGATCGACCGCGAGTATggcggcg                                                                                      >  4:408824/1‑138 (MQ=255)                                                    ggcggAGATGCCCTTGTCCCGAATATTGTCAGCAAAGTAAAACCGGCAGCGCCGTCGCTTTCATTGGGGACCGGATACGG‑CCCGACAGAAAACACGA‑CGTTTTCAACCAGTTTTCTGATCGACCGCGAGTATGGCGGct                                                                                      <  6:241313/139‑1 (MQ=255)                                                    cagacagagGCCCTTGTCCCGCTTATTGGCAGAAAATTAAAACAGGCAGCGCGGTCGCTTTCTTTGTGCACCGGATACGG‑CCCGACATAAACCACGA‑CGTTTTCAACCAGTTTTCTGATCGACCGCGAGTATGGCGGct                                                                                      <  3:135887/134‑1 (MQ=255)                                                        acaccgCCCTGGCCCCGCATATTGTCAGCAAAGTAAAACAGGCATCGCCGTCGCTTTAATTGGGGACCGTATACGG‑CCCGACATAAAACACGA‑CGTTTTCAACCAGTTTTCTGATCGACCGCGAGTATGGCGGCTCTAt                                                                                  <  4:368247/134‑1 (MQ=255)                                                           aTGCCCTTGTCCCGCATATTGTCAGCAAAGTAAAACAGGCATCGCCGTCGCTTTGATTGTGGAACGGATACGG‑CCCGACAGAAAACACGA‑CCTTTTCAACCAGTTTTATGATCGACCCCCAGTATGGCGGCTCTATacc                                                                               >  2:100384/1‑139 (MQ=255)                                                           aTGCCCTTGTCCCGCATATTGTCAGCAAAGTAAAACAGGCATCGCCGTCGCTTTAATTTTGGGAACGTTTCGG‑CCCCCCAGCAAAAAAGA‑CCTTTTAAACCATGTTTGAGTTAGCCCCCGAGTATTGCCGGTCTaaacc                                                                               >  6:28295/1‑135 (MQ=255)                                                                cTTGTCCCGCATATTGTCAGCAAAGTAAAACAGGCATCGCCGTCGCGTTAAATGTGGGACGGATACGG‑CCCCCCACAAAAACCGA‑CGGTTTCAACCACTTTTCTGATAGACCGCGCCGATTGCCGCGCGTTCCCAATCg                                                                          >  1:306557/1‑139 (MQ=255)                                                                         cATATTGTCAGCAAAGTAAAACAGGCATCGCCGTCGCTTTCATTTTGGAACGGATACGG‑CCCGACAGAAAACACGC‑CCTTTTGAACCAGTTTTCTGATCGACCGCCAGTATGGCGGCTCTATACCCATCCGGactgtgg                                                                 >  3:368247/1‑133 (MQ=255)                                                                         cATATTGTCAGCAAAGTAAAACAGGCATCGCCGTCGCGGTCATTGTGGGTCGGCTACGG‑CCCGCCACAGAACACGC‑CGGTTTCAACCAGTTTTATGATAGCCCGCGAGTATGGCGGCGCTATCCCAAACGGGGCGCCgc                                                                 >  7:168170/1‑138 (MQ=255)                                                                           aatTGTCAGCAACTTAAACCAGCCATCGCCGCCGCTTTCATTGTGGAACGGATACGG‑CCCCACAGAAAACACGA‑CGTTTTCAACCAGTTTTCTGATCGACCGCGAGTATGGCGGCTCTATCCCAATCGGGAAGCCGATc                                                               <  1:219423/138‑1 (MQ=255)                                                                              tGTCAGCAAAGTAAAACAGGCATCGCCGTCGCTTTAATTGTGGGAAGGATACGG‑CCCGACAGAAAACACGC‑CGTTTTCAACCACTTTTATGATCGACCGCGAGTTTGGCGGCGCTATCCCAAACGGGAAGCCGATCgg                                                             >  4:185928/1‑138 (MQ=255)                                                                                 aatcAAAGTAAAACAGGATTGGCCGTCGCTTGATTTGTGGAACGTATACGG‑CCCGACATAAAACACGA‑CGTTTTCAACCAGTTTTCTGATCGACCGCGAGTATGGCGGCTCTATCCCAATCGGGAAGCCGATCGGaaa                                                          <  3:348307/135‑1 (MQ=255)                                                                                    aaacgTAAAACCGCCAGCCCCGGCGCTTTCATTGGGCACCGGATCCGC‑CCCCACACAAAACACGA‑CGTTTTCAACCAGTTTTCTGATCGACCGCGAGTATGGCGGCTCTATCCCAATCGGGAAGCCGATCGGAAATTcc                                                      <  4:269367/135‑1 (MQ=255)                                                                                     gaaGTAAAACAGGCATCGCCGTCGCTTTCATTGTGGAACGGATACGG‑CCCGACAGAAAACACGA‑CGTTTTCAACCAGTTTTCTGATCGACCGCGAGTATGGCGGCTCTATCCCAATCGGGAAGCCGATCGGAAATTCCa                                                     <  3:209236/138‑1 (MQ=255)                                                                                     aacgTAAACCAGGCATCGCCGTCGCTTTCATTGGGGAACGGATACGG‑CCCGACACAAACCACGA‑CGTTTTCAACCAGTTTTCTGATCGACCGCGAGTATGGCGGCTCTATCCCAATCGGGAAGCCGATCGGAAATTCCa                                                     <  4:312254/136‑1 (MQ=255)                                                                                     aaaTTAAAACCGGCAGCGCGGGCGTTTTCATTGTGCAACGTA‑ACGGCCCCGACACAAAACACGA‑CGTTTTCAACCAGTTTTCTGATCGACCGCGAGTATGGCGGCTCTATCCCAATCGGGAAGCCGATCGGAAATTCCa                                                     <  4:13404/139‑1 (MQ=255)                                                                                     aaaGTAAAACAGGCTTCGCCGACGATTTCTTTTTGGACGGTAGCCGC‑CCCCACACAAAACACGA‑CGTTTTCAACCAGTTTTCTGATCGACCGCGAGTATGGCGGCTCTATCCCAATCGGGAAGCCGATCGGAAATTCCa                                                     <  5:28295/139‑1 (MQ=255)                                                                                     aaaGTAAAACAGGCATCGCCGTCGCTTTCATTGTGGAACGGATACGG‑CCCGACAGAAAACACGA‑CGTTTTCAACCAGTTTTCTGATCGACCGCGAGTATGGCGGCTCTATCCCAATCGGGAAGCCGATCGGAAATTCCa                                                     <  7:213979/139‑1 (MQ=255)                                                                                     aaaGAAAAACAGGCCTCGCCGTCGCTTTCATTGTGGACCGTTTACGG‑CCCCCCAGAAAACACGA‑CGTTTTCAACTAGTTTTCTGATCGACTGCGAGTATGGCGTCTCTATCCCAATCGGGAAGCCGATCGGAAATTCCa                                                     <  3:295049/139‑1 (MQ=255)                                                                                      aaGTAAAACAGGCATCGCCGTCGCTTTCATTGTGGAACGGATACGG‑CCCGACAGAAAACACGA‑CGTTTTCAACCAGTTTTCTGATCGACCGCGAGTATGGCGGCTCTATCCCAATCGGGAAGCCGATCGGAAATTCCAc                                                    <  1:220213/139‑1 (MQ=255)                                                                                       aGTAAAACAGGCATCGCCGTCGCGTTGATTTTGGGACGGATACGG‑CCCGCCCGAAAACACCC‑CGGTTTCAACCAGTTTTGTGGTCGACCGCGCGGATGGCGGGGCTATCCCAATCGGGGAGCCGCTCGGGAAttgcccg                                                   >  4:189050/1‑134 (MQ=255)                                                                                         tAAACCCGGCATCGCCGTCGCTTTCATTGGGCAACGTACAGGG‑CCCGACAGAAACCACGA‑CGTTTTCAACCAGTTTTCTGATCGACCGCGAGTATGGCGGCTCTATCCCAATCGGGAAGCCGATCGGAAATTCCACTGc                                                 <  2:228613/139‑1 (MQ=255)                                                                                          aaacccggCATCGCCGTCGCTTTCATTGTGGACCGGATACGG‑CCCGACATAAACCACGA‑CGTTTTCAACCAGTTTTCTGATCGACCGCGAGTATGGCGGCTCTATCCCAATCGGGAAGCCGATCGGAAATTCCACTGc                                                 <  3:316105/132‑1 (MQ=255)                                                                                          aaaaCAGGCATCGCCGGCGCTTTCATTGTGGAACGGATACGG‑CCCGACAGAAAACACGA‑CGTTTTCAACCAGTTTTCTGATCGACCGCGAGTATGGCGGCTCTATCCCAATCGGGAAGCCGATCGGAAATTCCACTGc                                                 <  3:150932/138‑1 (MQ=255)                                                                                                 gCATCGCCGTCGCTTTGATTTTGGAACGGATACGG‑CCCGACAGAAAACACGA‑CCTTTTAAACCAGTTTTCTGATAGACCGCGAGTATTGCGGCTCTAACCCAAACGGGAAGCCGATCGGAAATTACACTGCCAAtatta                                         >  4:209236/1‑139 (MQ=255)                                                                                                          gcGCTTTCATTGTGGAACGGATACGG‑CCCGACAGAAAACACGA‑CGTTTTCAACCAGTTTTCTGATCGACCGCGAGTATGGCGGCTCTATCCCAATCGGGAAGCCGATCGGAAATTCCACTGCCTATATTATGGATGagc                                <  8:260966/138‑1 (MQ=255)                                                                                                               agagttGTGTAATGTATACGG‑CCGGAAACAAAACACGA‑CGTTTTCAACCAGTTTTCTGATCGACCGCTAGTATGGCGGCTCTATCCCAATCGGGAAGCCGATCGGAAATTCCACTGCCTATATTATGGATGAgcagca                            <  4:321217/134‑1 (MQ=255)                                                                                                                tCATTGTGCAACGGATACGG‑CCCCACAGAAAACACGA‑CGTTTTAAACCAGTTTTCTGATCGACCGCGAGTATGGCGGCTCTATCCCAATCGGGAAGCCGATCGGAAATTCCACTGCCTATATTATGGATGAgcagca                            <  7:132693/137‑1 (MQ=255)                                                                                                                  aTTGTGGAACGGATACGG‑CCCGACAGAAAACACGA‑CGTTTTCAACCAGTTTTCTGATCGACCGCGAGTATGGCGGCTCTATCCCAATCGGGAAGCCGCTCGGAAATTACACTGCCTATATTATGGATGAGCAGCAATGc                        >  7:29529/1‑139 (MQ=255)                                                                                                                           cGGATACGG‑CCCGACAGAAAACACGA‑CGTTTTCAACCAGTTTTCTGATCGACCGCGAGTATGGCGGCTCTATCCCAATCGGGAAGCCGATCGGAAATTCCACTGCCTATATTATGGATGAGCAGCAATGCCTGCAGcc                >  3:82652/1‑138 (MQ=255)                                                                                                                              aTACGG‑CCCGACAGAAAACACGA‑CGTTTTCAACCAGTTTTCTGATCGACCGCGAGAATGGCGGCTCTATCCCAATCGGGAAGCCGATCGGAATTTCAACTGCCTATATAATGGATGAGCAGCAATGCCTGCAGCCAATc            >  3:35655/1‑139 (MQ=255)                                                                                                                              aTACGG‑CCCGACAGAAAACACGA‑CGTTTTCAACCAGTATTCTGATCGACAGCGAGTATGGCGGCTCTATCCCAATCGGGAAGCCGATCGGAAATTCCACTGCCTATATTATGGATGAGCAGCAATGCCTGCAGcctgtc            >  3:321217/1‑135 (MQ=255)                                                                                                                                aCGG‑CCCGACAGAAACCACGA‑CGTTTTCAACCAGTTTTCTGATCGACCGCGAGTATGGCGGCTCTATCCCAATCGGGAAGCCGATCGGAAATTCCACTGCCTATATTATGGATGAGCAGCAATGCCTGCAGCCAATCgg          <  1:100384/139‑1 (MQ=255)                                                                                                                                aCGG‑CCCGACAGAAAACACGA‑CGTTTTCAACCAGTTTTCTGATCGACCGCGAGTATGGCGGCTCTATCCCAATCGGGAAGCCGATCGGAAATTCCACTGCCTATATTATGGATGAGCAGCaa                           >  4:156392/1‑122 (MQ=255)                                                                                                                                aCGG‑CCCGACAGAAAACACGA‑CGTTTTCAACCAGTTTTCTGATCGACCGCGAGTATGGCGGCTCTATCCCAATCGGGAAGCCGATCGGAAATTCCACTGCCTATATTATGGATGAGCAGCaa                           <  3:156392/122‑1 (MQ=255)                                                                                                                                aCGG‑CCCGACAGAAAACACGA‑CGTTTTCAACCAGTTTTCTGATCGACCGCGAGTATGGCGGCTCTATCCCAATCGGGAAGCCGATCGGAAATTCCACTGCCTATATTATGGATGAGCAGCAATGCCTGCAGCCAATCgg          <  7:312760/139‑1 (MQ=255)                                                                                                                                   g‑CCCTACATAAAACAGGA‑CGTTTTCAACGAGTTTTGTGATCGACCGCGAGTATGGCGGCTCTATCCCACTCGGGAAGCCGATCGGAAAATCCACTGCCTTTATTATGGATGAGCAGCAATGGCTGCAGCCAATCGgcgc       <  3:364678/139‑1 (MQ=255)                                                                                                                                   g‑CCCGACACAAACCACGA‑CGTTTTCAACCAGTTTTCTGATCGACCGCGAGTATGGCGGCTCTATCCCAATCGGGAAGCCGATCGGAAATTCCACTGCCTATATTATGGATGAGCAGCAATGCCTGCAGCCAATCGgcgc       <  3:185928/139‑1 (MQ=255)                                                                                                                                   g‑CCCGACACAAAACACGA‑CGTTTTCAACCAGTTTTCTGATCGACCGCGAGTATGGCGGCTCTATCCCAATCGGGAAGCCGATCGGAAATTCCACTGCCTATATTATGGATGAGCAGCAATGGCTGCAGCCAATCGgcgc       <  3:370877/139‑1 (MQ=255)                                                                                                                                    cccccACAGAAACCACGA‑CGTTTTCAACCAGTTTTCTGATCGACCGCGAGTATGGCGGCTCTATCCCAATCGGGAAGCCGATCGGAAATTCCACTGCCTATATTATGGATGAGCAGCAATGCCTGCAGCCAATCGgcgc       <  5:296940/138‑1 (MQ=255)                                                                                                                                        gACAGAAAACACGA‑CGTTTTCAACCAGTTTTCTGATCGACCGCGAGTATGGCGGCTCTATCCCAATCGGGAAGCCGATCGGAAATTcc                                                      <  2:274588/88‑1 (MQ=255)                                                                                                                                        gACAGAAAACACGA‑CGTTTTCAACCAGTTTTCTGATCGACCGCGAGTATGGCGGCTCTATCCCAATCGGGAAGCCGATCGGAAATTcc                                                      >  1:274588/1‑88 (MQ=255)                                                                                                                                         aCAGAAAACACGA‑CGTTTTCAACCAGTTTTCTGATCGACCGCGAGTATGGCGGCTCTATCCCAATCGGGAAGCCGATCGGAAATTCCACTGCCTATATTATGGATGAGCAGCAATGCCTGCCGCCAATCGGCGCGCCTg  >  8:187424/1‑139 (MQ=255)                                                                                                                                         aCAGAAAACACGA‑CGTTTTCAACCAGTTTTCTGATCGACCGCGAGTATGGCGGCTCTATCCCAATCGGGAAGCCGATCGGAAATTCCACTGCCTATATTATGGATGAGCAGCAATGCCTGCAGCCAATCGGCGCGCCTg  >  6:20439/1‑139 (MQ=255)                                                                                                                                         |                                                                                                                                            CTTGCTGCAAAGGATGCGGGTATGTTCGGCACACTGCGCCATTTAATCATCGGCGGAGATGCCCTTGTCCCGCATATTGTCAGCAAAGTAAAACAGGCATCGCCGTCGCTTTCATTGTGGAACGGATACGG‑CCCGACAGAAAACACGA‑CGTTTTCAACCAGTTTTCTGATCGACCGCGAGTATGGCGGCTCTATCCCAATCGGGAAGCCGATCGGAAATTCCACTGCCTATATTATGGATGAGCAGCAATGCCTGCAGCCAATCGGCGCGCCTG  >  BS168_reference_genome/389871‑390144

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑