Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	BS168_reference_genome	143,659	C→T	100%	S100F (TCT→TTT)	rpsE →	ribosomal protein S5

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	BS168_reference_genome	143,659	0	C	T	100.0%	279.3 / NA	79	S100F (TCT→TTT)	rpsE	ribosomal protein S5
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (31/48);  total (31/48)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

AAGTACCAGAAGCGATTCGCAAAGCTGTTGAAGATGCGAAAAAGAATTTGATTGAAGTACCAATGGTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTCTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGATATCCTTTCTAAGTCTTTAGGTTCTAACACACCGATCAACATGATTCGTGCAACACTTCAAGGTTTAAG  >  BS168_reference_genome/143524‑143794                                                                                                                                        |                                                                                                                                        acttagcAGTAGCGATTCGCAAAGCTGTTGAAGATGCGAAAAAGAATTTGATTGAAGTACCAATGGTTGGAACTACAATTCCTCACGATATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGa                                                                                                                                      <  1:1637/133‑1 (MQ=255) acgTACCAGAAGCGATTCGCAAAGCTGTTGAAGATGCGAAAAAGAATTTGATTGAAGTACCAATGGTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGa                                                                                                                                      <  5:215715/137‑1 (MQ=255) aagTTCCAGAAGCGATTCGCAAAGCTGTTGAAGATGCTTAAAAGAATTTGATTGAAGTACCAATGGTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGa                                                                                                                                      <  8:46743/139‑1 (MQ=255) aagTACCAGAAGCGATTCGCAAAGCTGTTGAAGATGCGAAAAAGAATTTGATTGAAGTACCAATGGTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGa                                                                                                                                      <  7:167905/139‑1 (MQ=255) aagTACCAGAAGCGATTCGCAAAGCTGTTGAAGATGCGAAAAAGAATTTGATTGAAGTACCAATGGTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGa                                                                                                                                      <  1:308452/139‑1 (MQ=255) aagTACCAGAAGCGATTCGCAAAGCTGTTGAAGATGCGAAAAAGAATTTGATTGAAGTACCAATGGTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGa                                                                                                                                      <  5:101972/139‑1 (MQ=255) aagTACCAGAAGCGATTCGCAAAGCTGTTGAAGATGCGAAAAAGAATTTGATTGAAGTACCAATGGTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGa                                                                                                                                      <  4:327698/139‑1 (MQ=255) aagTACCAGAAGCGATTCGCAAAGCTGTTGAAGATGCGAAAAAGAATTTGATTGAAGTACCAATGGTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGa                                                                                                                                      <  4:277415/139‑1 (MQ=255) aagTACCAGAAGCGATTCGCAAAGCTGTTGAAGATGCGAAAAAGAATTTGATTGAAGTACCAATGGTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGa                                                                                                                                      <  4:191772/139‑1 (MQ=255) aagTACCAGAAGCGATTCGCAAAGCTGTTGAAGATGCGAAAAAGAATTTGATTGAAGTACCAATGGTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGa                                                                                                                                      <  4:101976/139‑1 (MQ=255) aagTACCAGAAGAGATTCGCAAAGCTGTTGAAGATGCGAAAAAGAATTTGATTGAAGTACCAATGGTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGa                                                                                                                                      <  3:297060/139‑1 (MQ=255)  agTACCAGAAGCGATTCGCAAAGCTGTTGAAGATGCGAAAAAGAATTTGATTGAAGTACCAATGGTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGa                                                                                                                                      <  6:306839/138‑1 (MQ=255)  agTACAAGAAGCGATTCGCAAAGGTGTTGAAGATGCGAAAAAGAATTTGATTGAAGTACCAATGGTTGGAACTACAATTCCACACGATATCATCGGACGTTTCGTTGAAGGTAACATCTTGTTAAAACATGCTTTTgt                                                                                                                                      <  8:49030/138‑2 (MQ=255)     aCCAGAAGCGATTCGCAAAGCTGTTGAAGATGCGAAAAAGAATTTGATTGAAGTACCAATGGTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAACCCTGCTTTTGAAgg                                                                                                                                   >  2:151194/1‑138 (MQ=255)     aCCAGAAGCGATTCGCAAAGCTGTTGAAGATGCGAAAAAGAATTTGATTGAAGTACCAATGGTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAgg                                                                                                                                   >  1:64905/1‑138 (MQ=255)     aCCAGAAGCGATTCGCAAAGCTGTTGAAGATGCGAAAAAGAATTTGATTGAAGTACCAATGGTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGt                                                                                                                                  >  5:45988/1‑139 (MQ=255)     aCCAGAAGCGATTCGCAAAGCTGATGAAGATGCGAAAAAGAATTTGATTGAAGTACCAATGGTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAgg                                                                                                                                   >  2:94994/1‑138 (MQ=255)           aGCGATTCGCAAAGCTGTTGAAGATGCGAAAAAGAATTTGATTGAAGTACCAATGGTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTg                                                                                                                              >  6:92919/1‑137 (MQ=255)               aTTCGCAAAGCTGTTGAAGATGCGAAAAAGAATTTGATTGAAGTACCAATGGTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGa                                                                                                                                      >  8:291471/1‑125 (MQ=255)               aTTCGCAAAGCTGTTGAAGATGCGAAAAAGAATTTGATTGAAGTACCAATGGTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGa                                                                                                                                      <  7:291471/125‑1 (MQ=255)               aTTCGCAAAGCTGTTGAAGATGCGAAAAAGAATTTGATTGAAGTACCAATGGTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTa                                                                                                                        >  5:249462/1‑139 (MQ=255)               aTTCGCAAAGCTGTTGAAGATGCGAAAAAGAATTTGATTGAAGTACCAATGGTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTa                                                                                                                        >  2:225736/1‑139 (MQ=255)                  cGCAAAGCTGTTGAAGATGCGAAAAAGAATTTGATTGAAGTACCAATGGTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATc                                                                                                                      <  7:181334/138‑1 (MQ=255)                  cGCAAAGCTGTTGAAGATGCGAAAAAGAATTTGATTGAAGTACCAATGGTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCg                                                                                                                     >  6:124680/1‑139 (MQ=255)                      aaGCTGTTGAAGATGCGAAAAAGAATTTGATTGAAGTACCAATGGTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTgg                                                                                                                 <  2:64905/139‑1 (MQ=255)                       aGCTGTTGAAGATGCGAAAAAGAATTTGATTGAAGTACCAATGGTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGa                                                                                                                <  3:393621/139‑1 (MQ=255)                            ttGAAGATGCGAAAAAGAATTTGATTGAAGTACCAATGGTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGccc                                                                                                           >  1:204720/1‑139 (MQ=255)                               aaGATGCGAAAAAGAATTTGATTGAAGTACCAATGGTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGt                                                                                                        <  3:206676/139‑1 (MQ=255)                               aaGATGCGAAAAAGAATTTGATTGAAGTACCAATGGTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGt                                                                                                        <  3:115475/139‑1 (MQ=255)                                   tGCGAAAAAGAATTTGATTGAAGTACCAATGGTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGt                                                                                                    >  4:97049/1‑139 (MQ=255)                                    gCGAAAAAGAATTTGATTGAAGTACCAATGGTTGGAACTACAATTCCACACGAAATCATCGGCCGTTTCGGTGCAGGTAACATCTTGTGAAAACCTGCTTTTGAAGGTACTGGAGTTATCTCTGGAGTCCCTGGACGTg                                                                                                   >  2:335751/1‑139 (MQ=255)                                    gCGAAAAAGAATTTGATTGAAGTACCAATGGTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTg                                                                                                   >  3:290900/1‑139 (MQ=255)                                     cGAAAAAGAATTTGATTGAAGTACCAATGGTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTg                                                                                                   <  5:92919/138‑1 (MQ=255)                                     cGAAAAAGAATTTGATTGAAGTACCAATGGTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTg                                                                                                   <  3:326899/138‑1 (MQ=255)                                     cGAAAAAGAATTTGATTGAAGTACCAATGGTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTg                                                                                                   <  2:204720/138‑1 (MQ=255)                                               tttGATTGAAGTACCAATGGTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGag                                                                                        <  2:175036/139‑1 (MQ=255)                                               tttGATTGAAGTACCAATGGTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGag                                                                                        <  1:73369/139‑1 (MQ=255)                                                   attgaAGTACCAATGGTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGagctag                                                                                    <  6:249462/139‑1 (MQ=255)                                                   attgaAGTAACAATGGGTGGAACTACAATTCCACACGAACTCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGagcta                                                                                     <  1:389297/138‑1 (MQ=255)                                                         gTACCAATGGTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGgtg                                                                              <  1:225736/139‑1 (MQ=255)                                                           aCCAATGGTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGgtgt                                                                             <  8:231568/138‑1 (MQ=255)                                                             cAATGGTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGGTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGc                                                                          <  3:60511/139‑1 (MQ=255)                                                             cAATGGTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGc                                                                          >  6:73265/1‑139 (MQ=255)                                                                  gTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGata                                                                     >  4:52301/1‑139 (MQ=255)                                                                    tGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGATATc                                                                   >  3:134028/1‑139 (MQ=255)                                                                    tGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGATATc                                                                   <  5:391518/139‑1 (MQ=255)                                                                     ggAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCAGTACTTGAGCTAGCTGGTGTAGCTGATATc                                                                   <  6:364554/138‑1 (MQ=255)                                                                      gAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACATGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGATATCCt                                                                 >  8:42188/1‑139 (MQ=255)                                                                       aaCTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGATATCCtt                                                                >  6:194795/1‑139 (MQ=255)                                                                           aCAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGATATCCTTTCt                                                             >  3:228839/1‑138 (MQ=255)                                                                            cAATTCCACACGAACTCCTCGGACGTTTCGGTTCAGGTAACATCTTGTTAACACCTGCTTTTGAAGGTACTGGAGTTCTCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGATATCCTTTCt                                                             <  1:335751/137‑1 (MQ=255)                                                                            cAATTCCACACGAAATCATCTGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGATATCCTTTCt                                                             <  1:383116/137‑1 (MQ=255)                                                                            cAATTCCACACGAAATCATCGGCCGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGCGGCCCTGTACGTGCGGTACTTGAGCTCGCTGGTGTAGCTGATATCCTTTCt                                                             >  8:103386/1‑137 (MQ=255)                                                                            cAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGATATCCTTTCt                                                             <  3:252780/137‑1 (MQ=255)                                                                                 ccACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGATATCCTTTCTAAGTCtt                                                      <  4:290900/139‑1 (MQ=255)                                                                                  cacaCGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGTGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGATATCCTTTCTAAGTCtt                                                      <  7:283233/138‑1 (MQ=255)                                                                                  cacaCGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGATATCCTTTCTAAGTCtt                                                      <  7:42188/138‑1 (MQ=255)                                                                                     acGAACTCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGATATCCTTTCTAAGTCTTTAgg                                                  <  6:45988/139‑1 (MQ=255)                                                                                     acGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGATATCCTTTCTAAGTCTTTAgg                                                  <  3:97049/139‑1 (MQ=255)                                                                                         aaTCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGATATCCTTTCTAAGTCTTTAGGTTc                                               >  8:323491/1‑138 (MQ=255)                                                                                                ggACGTTTCTGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGATATCCTTTCTAAGTCTTTAGGTTCTAacaca                                        <  7:325193/138‑1 (MQ=255)                                                                                                ggACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGATATCCTTTCTAAGTCTTTAGGTTCTAacaca                                        <  7:299927/138‑1 (MQ=255)                                                                                                ggACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGATATCCTTTCTAAGTCTTTAGGTTCTAacaca                                        <  1:151194/138‑1 (MQ=255)                                                                                                 gACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGgtg                                                                              >  6:64023/1‑99 (MQ=255)                                                                                                 gACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGgtg                                                                              <  5:64023/99‑1 (MQ=255)                                                                                                   cGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGATATCCTTTCTAAGTCTTTAGGTTCTAACACACCGa                                    <  3:203766/139‑1 (MQ=255)                                                                                                        cGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGATATCCTTTCTAAGTCTTTAGGTTCTAACACACCGATCAAc                               <  4:134028/139‑1 (MQ=255)                                                                                                                gTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTAt                                                                                                                       <  7:132264/43‑1 (MQ=255)                                                                                                                gTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTAt                                                                                                                       >  8:132264/1‑43 (MQ=255)                                                                                                                     aTCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGATATCCTTTCTAAGTCTTTAGGTTCTAACACACCGATCAACATGATTCGTGCaa                  >  6:248003/1‑139 (MQ=255)                                                                                                                     aTCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGATATCCTTTCTAAGTCTTTAGGTTCTAACACACCGATCAACATGATTCGTGCaa                  >  1:177063/1‑139 (MQ=255)                                                                                                                     aTCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGATATCCTTTCTAAGTCTTTAGGTTCTAACACACCGATCAACATGATTCGTGCaa                  <  2:177063/139‑1 (MQ=255)                                                                                                                       cTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGt                                                                                                        >  7:282491/1‑51 (MQ=255)                                                                                                                       cTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGt                                                                                                        <  8:282491/51‑1 (MQ=255)                                                                                                                          gTTAAAACCTTCTTTTGAAGGTACTGGAGTTATCGCTGGAGGACCTGTACGTTCGGTACTTGAGCTAGCTGGTGTAGCTCATATCCTTTCTAAGTCTTTAGGTTCTAACACACCGATCAACTTGATTCGTGCAACACtt             >  3:198216/1‑139 (MQ=255)                                                                                                                          gTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGATATCCTTTCTAAGTCTTTAGGTTCTAACCCACCGATCAACATGATTCGTGCAACACtt             >  8:135328/1‑139 (MQ=255)                                                                                                                          gTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGATATCCTTTCTAAGTCTTTAGGTTCTAACACACCGATCAACATGATTCGTGCAACACtt             >  1:2018/1‑139 (MQ=255)                                                                                                                                  cTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGATATCCTTTCTAAGTCTTTAGGTTCTAACACACCGATCAACATGATTCGTGCAACACTTCAAGGtt      >  2:407751/1‑138 (MQ=255)                                                                                                                                     cTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGATATCCTTTCTAAGTCTTTAGGTTCTAACACACCGATCAACATGATTCGTGCAACACTTCAAGGTTTAAg  >  4:158629/1‑139 (MQ=255)                                                                                                                                        |                                                                                                                                        AAGTACCAGAAGCGATTCGCAAAGCTGTTGAAGATGCGAAAAAGAATTTGATTGAAGTACCAATGGTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTCTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGATATCCTTTCTAAGTCTTTAGGTTCTAACACACCGATCAACATGATTCGTGCAACACTTCAAGGTTTAAG  >  BS168_reference_genome/143524‑143794

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑