Predicted mutation
evidence seq id position mutation freq annotation gene description
RA BS168_reference_genome 892,022 A→T 100% N196I (AAT→ATT)  malR → transcriptional activator of the Mal operon

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*BS168_reference_genome892,0220AT96.2% 176.2 / ‑1.5 52N196I (AAT→ATT) malRtranscriptional activator of the Mal operon
Reads supporting (aligned to +/- strand):  ref base A (1/1);  new base T (26/24);  total (27/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.62e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

AATTTGATGCGCGATGATTTTAAAGTGAACGATCTGTTTATCATCATTTCTCTTTCCGGAGAAACACCGGAGCTGATTCCTCAGGCAAGAATGCTTTCTGCAAAGGGTATTCCCTTTATTTCCATTACGAATTTAAAAAACAATGTCCTCGCCCAGCTGACTCCGCATAATCTTTATGCGACAAGCAAACCTGTGACATTAAGCGATAGAACGGAAATTGTCGCTTTTGCACCGTTTTTTCTCGTTGGCGAAGCATTGTTCCGCGC  >  BS168_reference_genome/891892‑892157
                                                                                                                                  |                                                                                                                                       
aatttGATGCGCGATGATTTTAAAGTGAACGATCTGTTTATCATCATTTCTCTTTCCGGAGAAACACCGGAGCTGATTCCTCAGGCAAGAATGCTTTCTGCAAAGGGTATTCCCTTTATTTCCATTACGATTTTaaaa                                                                                                                                  >  2:1170/1‑138 (MQ=255)
     gATGCGCGTTGATTTTAAAGTGAACGATCTGTTTATCATCATTTCTGTTTCCGGAGAAACACCGGAGCTGATTCCTCAGGCAAGAATGCTTTCTGCAAAGGGTATTCCCTTTATTTCCATTACGATTTTAAAAAACaa                                                                                                                             <  4:237075/138‑1 (MQ=255)
     gATGCGCGATGATTTTAAAGTGAACGATCTGTTTATCATCATTTCTCTTTCCGGAGAAACACCGGAGCTGATTCCTCAGGCAAGAATGCTTTCTGCAAAGGGTATTCCCTTTATTTCCATTACGATTTTAAAAAACAAt                                                                                                                            >  1:388649/1‑139 (MQ=255)
         cgcgATGATTTTAAAGTGAACGATCTGTTTATCATCATTTCTCTTTCCGGAGAAACACCGGAGCTGATTCCTCAGGCAAGAATGCTTTCTGCAAAGGGTATTCCCTTTATTTCCATTACGATTTTAAAAAACAATGTcc                                                                                                                        <  4:199910/139‑1 (MQ=255)
                      aaGTGAACGATCTGTTTATCATCATTTCTCTTTCCGGAGAAACACCGGAGCTGATTCCTCAGGCAAGAATGCTTTCTGCAAAGGGTATTCCCTTTATTTCCATTACGATTTTAAAAAACAATGTCCTCGCCCAGCTGAc                                                                                                           <  8:227691/139‑1 (MQ=255)
                       aGTGAACGATCTGTTTATCATCATTTCTCTTTCCGGAGAAACACCGGAGCTGATTCCTCAGGCAAGAATGCTTTCTGCAAAGGGTATTCCCTTTATTTCCATTACGATTTTAAAAAACAATGTCCTCGCCCAGCTGACt                                                                                                          <  1:384388/139‑1 (MQ=255)
                       aGTGAACGATCTGTTTATCATCATTTCTCTTTCCGGAGAAACACCGGAGCTGATTCCTCAGGCAAGAATGCTTTCTGCAAAGGGTATTCCCTTTATTTCCATTACGATTTTAAAAAACAATGTCCTCGCCCAGCTGACt                                                                                                          <  5:156139/139‑1 (MQ=255)
                          gAACGATCTGTTTATCATCATTTCACTTTCCTGAGAAACACCGGAGCTGATTCCTCAGGCAAGAATGCTTTCTGCAAAGGGTATTCCCTTTATTTCCATAACGATTTTAAAAAACAATGTCCTCGCCCAGCTGACTCCg                                                                                                       >  3:300986/1‑139 (MQ=255)
                           aaCGATCTGTTTATCATCATTTCTCTTTCCGGAGAAACACCGGAGCTGATTCCTCAGGCAAGAATGCTTTCTGCAAAGGGTATTCCCTTTATTTCCATTACGATTTTAAAAAACAATGTCCTCGCCCAGCTGACTCCg                                                                                                       >  3:404417/1‑138 (MQ=255)
                           aaCGATCTGTTTATCATCATTTCTCTTTCCGGAGAAACACCGGAGCTGATTCCTCAGGCAAGAATGCTTTCTGCAAAGGGTATTCCCTTTATTTCCATTACGATTTTAAAAAACAATGTCCTCGCCCAGCTGACTCCg                                                                                                       >  5:165970/1‑138 (MQ=255)
                           aaCGATCTGTTTATCATCATTTCTCTTTCCGGAGAAACACCGGAGCTGATTCCTCAGGCAAGAATGCTTTCTGCAAAGGGTATTCCCTTTATTTCCATTACGATTTTAAAAAACAATGTCCTCGCCCAGCTGACTCCg                                                                                                       >  7:298692/1‑138 (MQ=255)
                            aCGATCTGTTTATCATCATTTCTCTTTCCGGAGAAACACCGGAGCTGATTCCTCAGGCAAGAATGCTTTCTGCAAAGGGTATTCCCTTTATTTCCATTACGATTTTAAAAAACAATGTCCTCGCCCAGCTGACTCCGCa                                                                                                     <  8:303319/139‑1 (MQ=255)
                            aCGATCTGTTTATCATCATTTCTCTTTCCGGAGAAACACCGGAGCTGATTCCTCAGGCAAGAATGCTTTCTGCAAAGGGTATTCCCTTTATTTCCATTACGATTTTAAAAAACAATGTCCTCGCCCAGCTGACTCCGCa                                                                                                     <  2:388649/139‑1 (MQ=255)
                             cGATCTGTTTATCATCATTTCTCTTTCCGGAGAAACACCGGAGCTGATTCCTCAGGCAAGAATGCTTTCTGCAAAGTGTATTCCCTTTATTTCCATTACGATTTTAAAAAACAATGTCCTCGCCCAGCTGACTCCGCAt                                                                                                    <  4:390954/139‑1 (MQ=255)
                             cGATCTGTTTATCATCATTTCTCTTTCCGGAGAAACACCGGAGCTGATTCCTCAGGCAAGAATGCTTTCTGCAAAGGGTATTCCCTTTATTTCCATTACGATTTTAAAAAACAATGTCCTCGCCCAGCTGACTCCGCAt                                                                                                    <  1:326622/139‑1 (MQ=255)
                             cGATCTGTTTATCATCATTTCTCTTTCCGGAGAAACACCGGAGCTGATTCCTCAGGCAAGAATGCTTTCTGCAAAGGGTATTCCCTTTATTTCCATTACGATTTTAAAAAACAATGTCCTCGCCCAGCTGACTCCGCAt                                                                                                    <  5:318414/139‑1 (MQ=255)
                                     ttATCATCATTTCTCTTTCCGGAGAAACACCGGAGCTGATTCCTCAGGCAAGAATGCTTTCTGCAAAGGGTATTCCCTTTATTTCCATTACGATTTTAAAAAACAATGTCCTCGCCCAGCTGACTCCGCATAATCTTTa                                                                                            >  8:241791/1‑139 (MQ=255)
                                           tcatTTCTCTTTCCGGAGAAACACCGGAGCTGATTCCTCAGGCAAGAATGCTTTCTGCAAAGGGTATTCCCTTTATTTCCATTACGATTTTAAAAAACAATGTCCTCGCCCAGCTGACTCCGCATAATCTTTATGCGAc                                                                                      <  1:401608/139‑1 (MQ=255)
                                              tttCTCTTTCCGGAGAAACACCGGAGCTGATTCCTCAGGCAAGAATGCTTTCTGCAAAGGGTATTCCCTTTATTTCCATTACTATTTTAAAAAACAATGTCCTCGCCCAGCTGACTCCGCATAATCTTTATGCGACAAg                                                                                   >  3:175052/1‑139 (MQ=255)
                                              tttCTCTTTCCGGAGAAACACCGGAGCTGATTCCTCAGGCAAGAATGCTTTCTGCAAAGGGTATTCCCTTTATTTCCATTACGATTTTAAAAAACAATGTCCTCGCCCAGCTGACTCCGCATAATCTTTATGCGACAAg                                                                                   >  1:136071/1‑139 (MQ=255)
                                                 ctctTTCCGTAGAAACACCGGAGCTGATTCCTCAGGCAAGAATGCTTTCTGCAAAGGGTATTCACTTTATTTCCATTACGATTTTAAAAAACAATGTCCTCGCCCAGCTGACTCCGCATAATCTTTATGCGACAAGCa                                                                                 <  4:300986/138‑1 (MQ=255)
                                                      tCCGGAGAAACACCGGAGCTGATTCCTCAGGCAAGAATGCTTTCTGCAAAGGGTATTCCCTTTATTTCCATTACGATTTTAAAAAACAATGTCCTCGCCCAGCTGACTCCGCATAATCTTTATGCGACAAGCAAAc                                                                              <  6:342726/136‑1 (MQ=255)
                                                       ccGGAGAAACACCGGAGCTGATTCCTCAGGCAAGAATGCTTTCTGCAAAGGGTATTCCCTTTATTTCCATTACGATTTTAAAAAACAATGTCCTCGCCCAGCTGACTCCGCATAATCTTTATGCGACAAGCAAACCtg                                                                           <  8:298692/138‑1 (MQ=255)
                                                       ccGGAGAAACACCGGAGCTGATTCCTCAGGCAAGAATGCTTTCTGCAAAGGGTATTCCCTTTATTTCCATTACGATTTTAAAAAACAATGTCCTCGCCCAGCTGACTCCGCATAATCTTTATGCGACAAGCAAACCtg                                                                           <  2:133404/138‑1 (MQ=255)
                                                           agaAACACCGGAGCTGATTCCTCAGGCAAGAATGCTTTCTGCAAAGGGTATTCCCTTTATTTCCATTACGATTTTAAAAAACAATGTCCTCGCCCAGCTGACTCCGCATAATCTTTATGCGACAAGCAAACCTGTGACa                                                                      <  6:165970/139‑1 (MQ=255)
                                                                cacCGGAGCTGATTCCTCAGGCAAGAATGCTTTCTGCAAAGGGTATTCCCTTTATTTCCATTACGATTTTAAAAAACAATGTCCTCGCCCAGCTGACTCCGCATAATCTTTATGCGACAAGCAAACCTGTGACATTAAg                                                                 <  2:136071/139‑1 (MQ=255)
                                                                   cGGAGCTGATTCCTCAGGCAAGAATGCTTTCTGCAAAGGGTATTCCCTTTATTTCCATTACGATTTTAAAAAACAATGTCCTCGCCCAGCTGACTCCGCATAATCTTTATGCGACAAGCAAACCTGTGACATTAAGCGa                                                              <  7:241791/139‑1 (MQ=255)
                                                                   cGGAGCTGATTCCTCAGGCAAGAATGCTTTCTGCAAAGGGTATTCCCTTTATTTCCATTACGATTTTAAAAAACAATGTCCTCGCCCAGCTGACTCCGCATAATCTTTATGCGACAAGCAAACCTGTGACATTAAGCGa                                                              <  1:1170/139‑1 (MQ=255)
                                                                           aTTCCTCAGGCAAGAATGCTTTCTGCAAAGGGTATTCCCTTTATTTCCATTACGATTTTAAAAAACAATGTCCTCGCCCAGCTGACTCCGCATAATCTTTATGCGACAAGCAAACCTGTGACATTAATCGATAGAACg                                                       >  3:197935/1‑138 (MQ=255)
                                                                                  aGGCAAGAATGCTTTCTGCAAAGGGTATTCCCTTTATTTCCATTACTATTTTAAAAAACAATGTCCTCGCCCAGCTGACTCCGCATAATCTTTATGCGACAAGCAAACCTGTGACATTAAGCGATAGAACGGAAATGGt                                               >  5:328832/1‑139 (MQ=255)
                                                                                        gAATGCTTTCTGCAAAGGGTATTCCCTTTATTTCCATTACGATTTTAAAAAACAATGTCCTCGCCCAGCTGACTCCGCATAATCTTTATGCGACAAGCAAACCTGTGACATTAAGCGATAGAACGGAAATTGTCGCttt                                         >  6:35675/1‑139 (MQ=255)
                                                                                         aaTGCTTTCTGCAAAGGGTATTCCCTTTATTTCCATTACGATTTTAAAAAACAATGTCCTCGCCCAGCTGACTCCGCATAATCTTTATGCGACAAGCAAACCTGTGACATTAAGCGATAGAACGGAAATTGTCGCtttt                                        >  6:237167/1‑139 (MQ=255)
                                                                                              tttCTGCAAAGGGTATTCCCTTTATTTCCATTACGATTTTAAAAAACAATGTCCTCGCCCAGCTGACTCCGCATAATCTTTATGCGACAAGCAAACCTGTGACATTAAGCGATAGAACGGAAATTGTCGCTTTTGCAcc                                   >  5:73197/1‑139 (MQ=255)
                                                                                                  tGCAAAGGGTATTCCCTTTATTTCCATTACGATTTTAAAAAACAATGTCCTCGCCCAGCTGACTCCGCATAATCTTTATGCGACAAGCAAACCTGTGACATTAAGCGATAGAACGGAAATTGTCGCTTTTGCACCGttt                               >  7:374276/1‑139 (MQ=255)
                                                                                                       aGGGTATTCCCTTTATTTCCATTACGATTTTAAAAAACAATGTCCTCGCCCAGCTGACTCCGCATAATCTTTATGCGACAAGCAAACCTGTGACATTAAGCGATAGAACGGAAATTGTCGCTTTTGCACCGTTTTTtct                          >  2:136271/1‑139 (MQ=255)
                                                                                                            aTTCCCTTTATTTCCATTACGATTTTAAAAAACAATGTCCTCGCCCAGCTGACTCCGCATAATCTTTATGCGACAAGCAAACCTGTGACATTAAGCGATAGAACGGAAATTGTCGCTTTTGCACCGTTTTTTCTCGTTg                     >  8:58397/1‑139 (MQ=255)
                                                                                                            aTTCCCTTTATTTCCATTACGATTTTAAAAAACAATGTCCTCGCCCAGCTGACTCCGCATAATCTTTATGCGACAAGCAAACCTGTGACATTAAGCGATAGAACGGAAATTGTCGCTTTTGCACCGTTTTTTCTCGTTg                     >  6:364888/1‑139 (MQ=255)
                                                                                                            aTTCCCTTTATTTCCATTACGATTTTAAAAAACAATGTCCTCGCCCAGCTGACTCCGCATAATCTTTATGCGACAAGCAAACCTGTGACATTAAGCGATAGAACGGAAATTGTCGCTTTTGCACCGTTTTTTCTCGTTg                     >  6:240463/1‑139 (MQ=255)
                                                                                                                ccTTTATTTCCATTACGATTTTAAAAAACAATGTCCTCGCCCAGCTGACTCCGCATAATCTTTATGCGACAAGCAAACCTGTGACATTAAGCGATAGAACGGAAATTGTCGCTTTTGCACCGTTTTTTCTCGTTGGCg                  <  5:35675/138‑1 (MQ=255)
                                                                                                                ccTTTATTTCCATTACGATTTTAAAAAACAATGTCCTCGCCCAGCTGACTCCGCATAATCTTTATGCGACAAGCAAACCTGTGACATTAAGCGATAGAACGGAAATTGTCGCTTTTGCACCGTTTTTTCTCGTTGGCg                  <  5:240463/138‑1 (MQ=255)
                                                                                                                   ttATTTCCATTACGATTTTAAAAAACAATGTCCTCGCCCAGCTGACTCCGCATAATCTTTATGCGACAAGCAAACCTGTGACATTAAGCGATAGAACGGAAATTGTCGCTTTTGCACCGTTTTTTCTCGTTGGCGAAg               >  5:226833/1‑138 (MQ=255)
                                                                                                                   ttATTTCCATTACGATTTTAAAAAACAATGTCCTCGCCCAGCTGACTCCGCATAATCTTTATGCGACAAGCAAACCTGTGACATTAAGCGATAGAACGGAAATTGTCGCTTTTGCACCGTTTTTTCTCGTTGGCGAAg               >  5:193413/1‑138 (MQ=255)
                                                                                                                   ttATTTCCATTACGAATTTAAAAAACAATGTCCTCGCCCAGCTGACTCCGCATAATCTTTATGCGACAAGCAAACCTGTGACATTAAGCGATAGAACGGAAATTGTCGCTTTTGCACCGTTTTTTCTCGTTGGCGAAg               >  2:95541/1‑138 (MQ=255)
                                                                                                                    tATTTCCATTACGATTTTAAAAAACAATGTCCTCGCCCAGCTGACTCCGCATAATCTTTATGCGACAAGCAAACCTGTGACATTAAGCGATAGAACGGAAATTGTCGCTTTTGCACCGTTTTTTCTCGTTGGCGAAGCa             >  3:70518/1‑139 (MQ=255)
                                                                                                                          cATTACGATTTTAAAAAACAATGTCCTCGCCCAGCTGACTCCGCATAATCTTTATGCGACAAGCAAACCTGTGACATTAAGCGATAGAACGGAAATTGTCGCTTTTGCACCGTTTTTTCTCGTTGGCGAAGCATTGTTc       >  2:54791/1‑139 (MQ=255)
                                                                                                                            ttACGATTTTAAAAAACAATGTCCTCGCCCAGCTGACTCCGCATAATCTTTATGCGACAAGCAAACCTGTGACATTAAGCGATAGAACGGAAATTGTCGCTTTTGCACCGTTTTTTCTCGTTGGCGAAGCATTGTTCcg     <  4:46662/139‑1 (MQ=255)
                                                                                                                            ttACGATTTTAAAAAACAATGTCCTCGCCCAGCTGACTCCGCATAATCTTTATGCGACAAGCAAACCTGTGACATTAAGCGATAGAACGGAAATTGTCGCTTTTGCACCGTTTTTTCTCGTTGGCGAAGCATTGTTCcg     <  4:404417/139‑1 (MQ=255)
                                                                                                                            ttACGATTTTAAAAAACAATGTCCTCGCCCAGCTGACTCCGCATAATCTTTATGCGACAAGCAAACCTGTGACATTAAGCGATAGAACGGAAATTGTCGCTTTTGCACCGTTTTTTCTCGTTGGCGAAGCATTGTTCcg     >  7:348497/1‑139 (MQ=255)
                                                                                                                            ttACGATTTTAAAAAACAATGTCCTCGCCCAGCTGACTCCGCATAATCTTTATGCGACAAGCAAACCTGTGACATTAAGCGATAGAACGGAAATTGTCGCTTTTGCACCGTTTTTTCTCGTTGGCGAAGCATTGTTCcg     >  3:197107/1‑139 (MQ=255)
                                                                                                                            ttACGATTTTAAAAAACAATGTCCTCGCCCAGCTGACTCCGCATAATCTTTATGCGACAAGCAAACCTGTGACATTAAGCGATAGAACGGAAATTGTCGCTTTTGCACCGTTTTTTCTCGTTGGCGAAGCATTGTTCcg     >  2:354887/1‑139 (MQ=255)
                                                                                                                            ttACGAATTTAAAAAACAATGTCCTCGCCCAGCTGACTCCGCATAATCTTTATGCGACAAGCAAACCTGTGACATTAAGCGATAGAACGGAAATTGTCGCTTTTGCACCGTTTTTTCTCGTTGGCGAAGCATTGTTCcg     <  1:95541/139‑1 (MQ=255)
                                                                                                                               cGATTTTAAAAAACAATGTCCTCGCCCAGCTGACTCCGCATAATCTTTATGCGACAAGCAAACCTGTGACATTAAGCGATAGAACGGAAATTGTCGCTTTTGCACCGTTTTTTCTCGTTGGCGAAGCATTGTTCCgcgc  <  7:58397/139‑1 (MQ=255)
                                                                                                                                  |                                                                                                                                       
AATTTGATGCGCGATGATTTTAAAGTGAACGATCTGTTTATCATCATTTCTCTTTCCGGAGAAACACCGGAGCTGATTCCTCAGGCAAGAATGCTTTCTGCAAAGGGTATTCCCTTTATTTCCATTACGAATTTAAAAAACAATGTCCTCGCCCAGCTGACTCCGCATAATCTTTATGCGACAAGCAAACCTGTGACATTAAGCGATAGAACGGAAATTGTCGCTTTTGCACCGTTTTTTCTCGTTGGCGAAGCATTGTTCCGCGC  >  BS168_reference_genome/891892‑892157

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑