Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	BS168_reference_genome	1,690,758	C→G	74.0%	R214G (CGT→GGT)	codY →	transcriptional regulator, GTP and BCAA‑dependent

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	BS168_reference_genome	1,690,758	0	C	G	74.0%	86.6 / 31.1	50	R214G (CGT→GGT)	codY	transcriptional regulator, GTP and BCAA‑dependent
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base G (17/20);  minor base A (6/7);  total (23/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.03e-01

AAAGCTGTCGTACAAATGGCTATCAGCTCGCTTTCTTACAGTGAGCTTGAAGCAATTGAGCACATTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCCGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATCGAGTCTAGATCATTAGGAATGAAAGGTACTTATATCAAGGTACTAAACAACAAATTCCTAATTGAATTAGAA  >  BS168_reference_genome/1690623‑1690880                                                                                                                                        |                                                                                                                           aaaGCTGTCGTACAAATGGCTATCAGCTCGCTTTCTTACAGTGAGCTTGAAGCAATTGAGCACATTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCGGtt                                                                                                                         <  2:76626/139‑1 (MQ=255)    gCTGTCGTACAAATGGCTATCAGCTCGCTTTCTTACAGTGAGCTTGAAGCAATTGAGCACATTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCGGTTCTg                                                                                                                      <  3:27347/139‑1 (MQ=255)             cAAATGGCTATCAGCTCGCTTTCTTACAGTGAGCTTGAAGCAATTGAGCACATTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCAGTTCTGTTATtgtg                                                                                                              <  6:6454/138‑1 (MQ=255)               aagtggtATCAGCTCGCTTTCTTACAGTGAGGTTGAAGCAATTGAGCACAGTTTTGAGCAGCTTGAGGGTTATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCGGTTCTGTTATTGTGaa                                                                                                            <  4:348572/132‑1 (MQ=255)                  ggCTATCAGCTCGCTTTCTTACAGTGAGCTTGAAGCAATTGAGCACATTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCGGTTCTGTTATTGTGAACGCa                                                                                                        >  6:109161/1‑139 (MQ=255)                     tATCAGCTCGCTTTCTTACAGTGAGCTTGAAGCAATTGAGCACATTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCAGTTCTGTTATTGTGAACGCACt                                                                                                      >  6:145572/1‑138 (MQ=255)                       tCAGCTCGCTTTCTTACAGTGAGCTTGAAGCAATTGAGCACATTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCGGTTCTGTTATTGTGAACGCACTCa                                                                                                    <  4:110108/138‑1 (MQ=255)                        cAGCTCGCGTTCTTACAGTGAGCTTGAAGCAATTGAGCACATTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCGGTTCTGTTATTGTGAACGCACTCAGa                                                                                                  <  6:343021/139‑1 (MQ=255)                           cTCGATTTCTTACAGTGTGCTTGAAGCAATTTAGCACATTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCGGTTCTGTTATTGTGAACGCACTCAGAAAg                                                                                               <  1:373491/139‑1 (MQ=255)                                  tCTTACAGTGAGCTTGAAGCAATTGAGCACATTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCAGTTCTGTTATTGTGAACGCACTCAGAAAGCTGga                                                                                          <  1:17152/137‑1 (MQ=255)                                     tACAGTGAGCTTGAAGCAATTGAGCACATTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCAGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAgcg                                                                                     >  3:401337/1‑139 (MQ=255)                                       cAGTGATCTTGAATCAATTGAGCACATTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACGGTGTCGGCATTACCAGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAgcgc                                                                                    <  6:161415/138‑1 (MQ=255)                                       cAGTGAGCTTGAAGCAATTGAGCACATTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCGGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAgcgc                                                                                    <  6:329393/138‑1 (MQ=255)                                          tGAGCTTGAAGCAATTGAGCACATTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCGGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGTGCGCCGgt                                                                                >  5:21149/1‑139 (MQ=255)                                            atcTTGAAGCAATTGAGCACATTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCGGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGgtg                                                                               <  2:292662/136‑1 (MQ=255)                                            atcTTGAAGCAATTGAGCACATTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGATGACCTTGTCGGCATTACCGGTTCTGTTATTGTTAACTAACTCAGAAAGCTGTAGAGCGCCGgtgt                                                                              >  4:5109/3‑139 (MQ=255)                                            aGCTTGAAGCAATTGAGCACATTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGCCCGTGTCGGCTTTACCGGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGgtgt                                                                              >  5:215522/1‑139 (MQ=255)                                            aGCTTGAAGCAATTGAGCACATTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCGGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGCGCGCCGgtgt                                                                              >  2:369384/1‑139 (MQ=255)                                            aGCTTGAAGCAATTGAGCACATTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCGGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGgtgt                                                                              >  2:364144/1‑139 (MQ=255)                                            aGCTTGAAGCAATTGAGCACATTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCGGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGgtgt                                                                              >  4:262996/1‑139 (MQ=255)                                            aGCTTGAAGCAATTGAGCACATTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCGGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGgtgt                                                                              >  5:62279/1‑139 (MQ=255)                                            aGCTTGAAGCAATTGAGCACATTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCGGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGgtg                                                                               <  6:62279/138‑1 (MQ=255)                                            aGCTTGAAGCAATTGAGCACATTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCAGTTCTGTTATTGTGAACGCACTCAGAACGCTGGAGAGCGCCGgtgt                                                                              >  8:181815/1‑139 (MQ=255)                                                 gAAGCAATTGAGCACATTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCGGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTAt                                                                           <  3:5109/137‑1 (MQ=255)                                                 gAAGCAATTGAGCACATTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCGGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATc                                                                          <  7:238486/138‑1 (MQ=255)                                                 gAAGCAATTGAGCACATTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCGGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATc                                                                          <  3:262996/138‑1 (MQ=255)                                                  aaGCAATTGAGCACATTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCGGtt                                                                                                                         >  4:158365/1‑90 (MQ=255)                                                  aaGCAATTGAGCACATTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCGGtt                                                                                                                         <  3:158365/90‑1 (MQ=255)                                                  aaGCAATTGAGCACATTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCGGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATc                                                                          >  1:408349/1‑137 (MQ=255)                                                        ttGAGCACATTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCAGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATCGAGTc                                                                     >  4:69170/1‑136 (MQ=255)                                                        ttGAGCACATTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCAGTTCTGTTATTGTGAACGAACTCAGAAAGCTGGAGAGCTCCGGTGTTATCTAGTc                                                                     >  5:232975/1‑136 (MQ=255)                                                        ttGAGAACATTTTTGAGGAGCTTGACGCAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCGGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATCGAGTCTAg                                                                  <  8:105569/139‑1 (MQ=255)                                                             cacaTTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCGGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATCGAGTCTAGATCAt                                                             >  4:296952/1‑139 (MQ=255)                                                                       aggagCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCAGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATCGAGTCTAGATCATTAGGAATGaa                                                   <  4:401337/139‑1 (MQ=255)                                                                         gagCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCGGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATCGAGTCTAGATCATTAGGAATGAAAg                                                 <  1:304303/139‑1 (MQ=255)                                                                          agCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCGGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATc                                                                          <  4:46151/113‑1 (MQ=255)                                                                          agCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCGGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATc                                                                          >  3:46151/1‑113 (MQ=255)                                                                            cTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCGGtt                                                                                                                         <  7:396870/64‑1 (MQ=255)                                                                            cTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCGGtt                                                                                                                         >  8:396870/1‑64 (MQ=255)                                                                            cTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCGGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATCGAGTCTAGATCATTAGGAATGAAAGGTa                                              >  7:11035/1‑139 (MQ=255)                                                                               gACGGAACTGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCGGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCTGTGTTATCGAGTCTAGATCATAAGGAATGAAAGGTACt                                            <  6:324147/138‑1 (MQ=255)                                                                                     aaTGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCGGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATCGAGTCTAGATCATTAGGAATGAAAGGTACTTATATCa                                     <  5:109161/139‑1 (MQ=255)                                                                                     aaTGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCAGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATCGAGTCTAGATCATTAGGAATGAAAGGTACTTATATCa                                     <  3:69170/139‑1 (MQ=255)                                                                                      aTGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCAGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATCGAGTCTAGATCATTAGGAATGaa                                                   >  4:189116/1‑124 (MQ=255)                                                                                      aTGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCAGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATCGAGTCTAGATCATTAGGAATGaa                                                   <  3:189116/124‑1 (MQ=255)                                                                                         aaGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCGGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATCGAGTCTAGATCATTAGGAATGAAAGGTACTTATATCAAgg                                  <  7:110964/138‑1 (MQ=255)                                                                                                               aaTTGCTGACCGTGTCGGCATTACCGGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATCGAGTCTAGATCATTAGGAATGAAAGGTACTTATATCAAGGTACTAAACAACAAATTCCTAAtt           >  6:351311/1‑139 (MQ=255)                                                                                                                   gCTGACCGTGTCGGCATTACCGGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATCGAGTCTAGATCATTAGGAATGAAAGGTACTTATATCAAGGTACTAAACAACAAATTCCTAATTGAAt       >  1:153305/1‑139 (MQ=255)                                                                                                                   gCTGACCGTGTCGGCATTACCGGTTCTGTTATTGTCAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATCGAGTCTAGATCATTAGGAATGAAAGGTACTTATATCAAGGTACTAAACAACAAATTCCTAATTGAAt       >  3:254432/1‑139 (MQ=255)                                                                                                                        ccGTGTCGGCATTACCAGTTCTGTTATTGGGACCGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATCGAGTCTAGATCATTAGGAATGAAAGGTACTTATATCAAGGTACTAAACAACAAATTCCTAATTGAATTAGaa  <  7:181815/139‑1 (MQ=255)                                                                                                                                        |                                                                                                                           AAAGCTGTCGTACAAATGGCTATCAGCTCGCTTTCTTACAGTGAGCTTGAAGCAATTGAGCACATTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCCGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATCGAGTCTAGATCATTAGGAATGAAAGGTACTTATATCAAGGTACTAAACAACAAATTCCTAATTGAATTAGAA  >  BS168_reference_genome/1690623‑1690880

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑