Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	BS168_reference_genome	1,988,649	A→G	7.8%	P533P (CCT→CCC)	ppsB ←	nonribosomal plipastatin synthetase B involved in synthesis of plipastatin

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	BS168_reference_genome	1,988,649	0	A	G	7.8%	150.8 / 4.6	51	P533P (CCT→CCC)	ppsB	nonribosomal plipastatin synthetase B involved in synthesis of plipastatin
Reads supporting (aligned to +/- strand):  ref base A (19/28);  new base G (2/2);  total (21/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01

AAGGAAATAAGGTCTTCTTCTATCAGCAATTGAGCCGCTTGACTGTCCTTCAGAAGAAAAGAAAGACGTTCTGCTGGAAAATGTGGATCAAGCGGCATATAAGCGCCGCCTGCTTTCCAAATCCCAAGCAGAGCTGCAGGCATTTCCATTGACCGGCCCATCATTACAGCAACGATATCCCCAGATCCAACGCCTTTTTCTATCAGGGCGTGCGCAATCTGATTGGCTCTTACTTGTAACTGGCGATAGGTCCAGCACTCGTTTCCCATTTTCAA  >  BS168_reference_genome/1988512‑1988786                                                                                                                                          |                                                                                                                                          aaGGAAATAAGGTCTTCTTCTATCAGCAATTGAGCCGCTTGACTGTCCTTCAGAAGAAAAGAAAGACGTTCTGCTGGAAAATGTGGATCAAGCGGCATATAAGCGCCGCCTGCTTTCCAAATCCCAAGCAGAGCTGCAg                                                                                                                                          >  3:233482/1‑139 (MQ=255)    taaaaaaGGTCTTCTTCAAACAGCAATTGCGCCGGTTGCATGTCCTTCATAACAAAAGAAACACGTTCTGCGGGAAAATGTGGATCAAGCGGCATATAAGCGCCGCCTGCTTTCCAAATCCCAAGCAGAGCTGCAGGCa                                                                                                                                       <  6:63359/138‑1 (MQ=255)     aaaTACGGTCTGCTTCTAGCAGCAATTGAGCCGCTGGACTGTCCTGCAGAAGAAAAGAAAGCCGTTCTGCTGGAAAATGTGGATCAAGCGGCATATAAGCGCCGCCTGCTTTCCAAATCCCAAGCAGAGCTGCAGGCAt                                                                                                                                      <  6:405600/139‑1 (MQ=255)     aaaTAAGGTCTTCTTCTATCCGCAATTGAGCCGCTTGACTGTCCTACAGAACAAAAGAAAGACGTTCTGCTGGAAAATGTGGATCAAGCGGCATATAAGCGCCGCCTGCTTTCCAAATCCCAAGCAGAGCTGCAGGCAt                                                                                                                                      <  5:46151/139‑1 (MQ=255)     aaaTAAGGTCTTCTTCTATCAGCAATTGAGCCGCTTGACTGTCCTTCAGAAGAAAAGAAAGACGTTCTGCTGGAAAATGTGGATCAAGCGGCATATAAGCGCCGCCTGCTTTCCAAATCCCAAGCAGAGCTGCAGGCAt                                                                                                                                      <  1:269/139‑1 (MQ=255)     aaaTAAGGTCTTCTTCAATCAGCAATTGAGCCGCTTGACTGTCCTACACAACAAAAGAAACACGTTCTGCTGGAAAATGTGGATCAAGCGGCATATAAGCGCCGCCTGCTTTCCAAATCCCAAGCAGAGCTGCAGGCAt                                                                                                                                      <  4:107960/139‑1 (MQ=255)      aaTAAGGTCTTCTTCTATCAGCAATTGAGCCGCTTGACTGTCCTTCAGAAGAAAAGAAAGACGTTCTGCTGGAAAATGTGGATCAAGCGGCATATAAGCGCCGCCTGCTTTCCAAATCCCAAGCAGAGCTGCAGGCAt                                                                                                                                      <  4:233482/138‑1 (MQ=255)               ttcttctATCAGCAATTGAGCCGCTTGACTGTCCTTCAGAAGAAAAGAAAGACGTTCTGCTGGAAAATGTGGATCAAGCGGCATATAAGCGCCGCCTGCTTTCCAAATCCCAAGCAGAGCTGCAGGCATTTCCATTGAc                                                                                                                            >  7:164387/1‑139 (MQ=255)                 cttctATCAGCAATTGAGCCGCTTGACTGTCCTTCAGAAGAAAAGAAAGACGTTCTGCTGGAAAATGTGGATCAAGCGGCATATAAGCGCCGCCTGCTTTCCAAATCCCAAGCAGAGCTGCAGGCATTTCCATTGAc                                                                                                                            <  2:14910/137‑1 (MQ=255)                 cttctATCAGCAATTGAGCCGCTTGACTGTCCTTCAGAAGAAAAGAAAGACGTTCTGCTGGAAAATGTGGATCAAGCGGCATATAAGCGCCGCCTGCTTTCCAAATCCCAAGCAGAGCTGCAGGCATTTCCATTGACCg                                                                                                                          >  5:401357/1‑139 (MQ=255)                    ctATCAGCAATTGAGCCGCTTGACTGTCCTTCAGAAGAAAAGAAAGACGTTCTGCTGGAAAATGTGGATCAAGCGGCATATAAGCGCCGCCTGCTTTCCAAATCCCAAGCAGAGCTGCAGGCATTTCCATTGACCgg                                                                                                                         <  5:139581/137‑1 (MQ=255)                       tCAGCAATTGCGCCGCTTGACTGTCCTTCAGAAGAAAACAAAGACGTTCTGCTGGAAAATGTGGATCAAGCGGCATATAAGCGCCGCCTGCTTTCCAAATCCCAAGCAGAGCTGCAGGCATTTCCATTGACCGGcc                                                                                                                       <  7:265673/136‑1 (MQ=255)                       tCAGCAATTGAGCCGCTTGACTGTCCTTCAGAAGAAAAGAAAGACGTTCTGCTGGAAAATGTGGATCAAGCGGCATATAAGCGCCGCCTGCTTTCCAAATCCCAAGCAGAGCTGCAGGCa                                                                                                                                       <  5:160115/120‑1 (MQ=255)                       tCAGCAATTGAGCCGCTTGACTGTCCTTCAGAAGAAAAGAAAGACGTTCTGCTGGAAAATGTGGATCAAGCGGCATATAAGCGCCGCCTGCTTTCCAAATCCCAAGCAGAGCTGCAGGCa                                                                                                                                       >  6:160115/1‑120 (MQ=255)                       tCAGCAATTGAGCCGCTTGACTGTCCTTCAGAAGAAAAGAAAGACGTTCTGCTGGAAAATGTGGATCAAGCGGCATATAAGCGCCGCCTGCTTTCCAAATCCCAAGCAGAGCTGCAGGCATTTCCATTGACCGGCCcat                                                                                                                    >  5:141692/1‑139 (MQ=255)                              ttGAGCCGCTTGACTGTCCTTCAGAAGAAAAGAAAGACGTTCTGCTGGAAAATGTGGATCAAGCGGCATATAAGCGCCGCCTGCTTTCCAAATCCCAAGCAGAGCTGCAGGCATTTACATTGACCGGCCCATCATTa                                                                                                               >  1:61620/1‑137 (MQ=255)                                 aGCCGCTTGACTGTCCTTCAGAAGAAAAGAAAGACGTTCTGCTGGAAAATGTGGATCAAGCGGCATATAAGCGCCGCCTGCTTTCCAAATCCCAAGCAGAGCTGCAGGCATTTCCATTGACCGGCCCATCATTACAGc                                                                                                           >  3:78660/1‑138 (MQ=255)                                 aGCCGCTTGACTGTCCTTCAGAAGAAAAGAAAGACGTTCTGCTGGAAAATGTGGATCAAGCGGCATATAAGCGCCGCCTGCTTTCCAAATCCCAAGCAGAGCTGCAGGCATTTCCATTGACCGGCCCATCATTACAGCa                                                                                                          <  8:164387/139‑1 (MQ=255)                                  gCCGCTTGACTGTCCTTCAGAAGAAAAGAAAGACGTTCTGCTGGAAAATGTGGATCAAGCGGCATATAAGCGCCGCCTGCTTTCCAAATCCCAAGCAGAGCTGCAGGCATTTCCATTGACCGGCCCATCATTACAGCaa                                                                                                         <  7:94220/139‑1 (MQ=255)                                      cTTGACTGTCCTTCAGAAGAAAAGAAAGACGTTCTGCTGGAAAATGTGGATCAAGCGGCATATAAGCGCCGCCTGCTTTCCAAATCCCAAGCAGAGCTGCAGGCATTTCCATTGACCGGCCCATCATTACAGCAACGat                                                                                                     <  8:58960/139‑1 (MQ=255)                                              tCCTTCAGAAGAAAAGAAAGACGTTCTGCTGGAAAATGTGGATCAAGCGGCATATAAGCGCCGCCTGCTTTCCAAATCCCAAGCAGAGCTGCGGGCATTTCCATTGACCGGCCCATCATTACAGCAACGATATCCCCAg                                                                                             >  4:196737/1‑139 (MQ=255)                                              tCCTTCAGAAGAAAAGAAAGACGTTCTGCTGGAAAATGTGGATCAAGCGGCATATAAGCGCCGCCTGCTTTCCAAATCCCAAGCAGAGCTGCAGGCa                                                                                                                                       <  7:294833/97‑1 (MQ=255)                                              tCCTTCAGAAGAAAAGAAAGACGTTCTGCTGGAAAATGTGGATCAAGCGGCATATAAGCGCCGCCTGCTTTCCAAATCCCAAGCAGAGCTGCAGGCa                                                                                                                                       >  8:294833/1‑97 (MQ=255)                                              tCCTTCAGAAGAAAAGAAAGACGTTCTGCTGGAAAATGTGGATCAAGCGGCATATAAGCGCCGCCTGCTTTCCAAATCCCAAGCAGAGCTGCAGGCATTTCCATTGACCGGCCCATCATTACAGCAACGATATcccc                                                                                               <  8:340191/137‑1 (MQ=255)                                              tCCGTCAGTAGAAAAGAAAGACGTTCTGCTGGAAAAAGTGGATCAACCGGCATATAACCGCCGCCTGCTTTCCAAAACCCAACCAGAGCTGCGGGCATTTCCATTGCCCGGCCCATCAATTCAGCAACCCTATccc                                                                                                >  4:203207/1‑136 (MQ=255)                                                cTTCAGAAGAAAAGAAAGACGTTCTGCTGGAAAATGTGGATCAAGCGGCATATAAGCGCCGCCTGCTTTCCAAATCCCAAGCAGAGCTGCAgg                                                                                                                                         >  6:1518/1‑93 (MQ=255)                                                cTTCAGAAGAAAAGAAAGACGTTCTGCTGGAAAATGTGGATCAAGCGGCATATAAGCGCCGCCTGCTTTCCAAATCCCAAGCAGAGCTGCAgg                                                                                                                                         <  5:1518/93‑1 (MQ=255)                                                cTTCAGAAGAAAAGAAAGACGTTCTGCTGGAAAATGTGGATCAAGCGGCATATAAGCGCCGCCTGCTTTCCAAATCCCAAGCAGAGCTGCAGGCATTTCCATTGACCGGCCCATCATTACAGCAACGATATccc                                                                                                >  1:198799/1‑134 (MQ=255)                                                     gaataaAAGAAAGCCGTTCTGCTGGAAAATGGGGCTCAAGCGGCATATAAGCGCCGCCAGCTTTCCAAATCCCAAGCAGAGCTGCAGGCATTTCCATTGACCGGCCCATCATTACAGCAACGATATCCCCAGATCCAAc                                                                                      <  6:96339/139‑1 (MQ=255)                                                       agaaaagaaaGACGTTCTGCTGGAAAATGTGGATCAAGCGGCATATAAGCGCCGCCTGCTTTCCAAATCCCAAGCAGAGCTGCAGGCATTTCCATTGACCGGCCCATCATTACAGCAACGATATCCCCAGATCCAACGc                                                                                    <  6:56345/139‑1 (MQ=255)                                                        gaaaagaaaGACGTTCTGCTGGAAAATGTGGATCAAGCGGCATATAAGCGCCGCCTGCTTTCCAAATCCCAAGCAGAGCTGCAGGCATTTCCATTGACCGGCCCATCATTACAGCAACGATATCCCCAGATCCAACGcc                                                                                   >  8:301445/1‑139 (MQ=255)                                                           aagaaagaCGTTCTGCTGGAAAATGTGGATCAAGCGGCATATAAGCGCCGCCTGCTTTCCAAATCCCAAGCAGAGCTGCAGGCATTTCCATTGACCGGCCCATCATTACAGCAACGATATCCCCAGATCCAACGCCttt                                                                                <  7:301445/139‑1 (MQ=255)                                                                  aCGTTCTGGTGTAAAATGTGCATCAAGCGGCTTATAAGCGCCGCCTGCTTTCCAAATCCCAAGCAGAGCTGCAGGCATTTCCATTGACCGGCCCATCATTACAGCAACGATATCCCCAGATCCAACGCCTTTTTCTATc                                                                         <  2:191216/139‑1 (MQ=255)                                                                     ttCTGCTGGAAAATGTGGATCAAGCGGCATATAAGCGCCGCCTGCTTTCCAAATCCCAAGCAGAGCTGCAGGCATTTCCATTGACCGGCCCATCATTACAGCAACGATATCCCCAGATCAAACTCCTTTTTCTATCAgg                                                                      >  2:235508/1‑139 (MQ=255)                                                                       ctgctgGAAAATGTGGATCAAGCGGCATATAAGCGCCGCCTGCTTTCCAAATCCCAAGCAGAGCTGCAGGCATTTCCATTGACCGGCCCATCATTACAGCAACGATATCCCCAGATCCAACGCCTTTTTCTATCAggg                                                                     <  2:52039/138‑1 (MQ=255)                                                                              aaacagtgGAGCAAGCGGCATATAAGCGCCGCCTGCTTTCCAAATCCCAAGCAGAGCTGCAGGCATTTCCATTGACCGGCCCATCATTACAGCAACGATATCCCCAGATCCAACGCCTTTTTCTATCAGGGCGTGCGCa                                                             <  1:149403/134‑1 (MQ=255)                                                                                  tgtgGATCAAGCGGCATATAAGCGCCGCCTGCTTTCCAAATCCCAAGCAGAGCTGCAGGCATTTCCATTGACCGGCCCATCATTACAGCAACGATATCCCCAGATCCAACGCCTTTTTCTATCAGGGCGTGCGCAATCt                                                         >  2:31025/1‑139 (MQ=255)                                                                                      gATCAAGCGGCATATAAGCGCCGCCTGCTTTCCAAATCCCAAGCAGAGCTGCAGGCATTTCCATTGACCGGCCCATCATTACAGCAACGATATCCCCAGATCCAACGCCTTTTTCTATCAGGGCGTGCGCAATCTGAt                                                      <  7:278290/138‑1 (MQ=255)                                                                                                      aGCGCCGCCTGCTTTCCAAATCCCAAGCAGAGCTGCGGGCATTTCCATTGACCGGCCCATCATTACAGCAACGATATCCCCAGATCCAACGCCTTTTTCTATCAGGGCGTGCGCAATCTGATTGGCTCTTACTTGTaa                                      <  3:196737/138‑1 (MQ=255)                                                                                                      aGCGCCGCCTGCTTTCCAAATCCCAAGCAGAGCTGCGGGCATTTCCATTGACCGGCCCATCATTACAGCAACGATATCCCCAGATCCAACGCCTTTTTCTATCAGGGCGTGCGCAATCTGATTGGCTCTTACTTGTaa                                      <  3:203207/138‑1 (MQ=255)                                                                                                      aGCGCCGCCTGCTTTCCAAATCCCAAGCAGAGCTGCAGGCATTTCCATTGACCGGCCCATCATTACAGCAACGATATCCCCAGATCCAACGCCTTTTTCTATCAGGGCGTGCGCAATCTGATTGGCTCTTACTTGTaa                                      <  6:401357/138‑1 (MQ=255)                                                                                                          ccgccTGCTTTCCAAATCCCAAGCAGAGCTGCAGGCATTTCCATTGAc                                                                                                                            >  3:11167/1‑48 (MQ=255)                                                                                                          ccgccTGCTTTCCAAATCCCAAGCAGAGCTGCAGGCATTTCCATTGAc                                                                                                                            <  4:11167/48‑1 (MQ=255)                                                                                                          ccgccTGCTTTCCAAATCCCAAGCAGAGCTGCAGGCATTTCCATTGACCGGCCCATCATTACAGCAACGATATCCCCAGATCCAACGCCTTTTTCTATCAGGGCGTGCGCAATCTGATTGGctc                                                <  7:103392/124‑1 (MQ=255)                                                                                                          ccgccTGCTTTCCAAATCCCAAGCAGAGCTGCAGGCATTTCCATTGACCGGCCCATCATTACAGCAACGATATCCCCAGATCCAACGCCTTTTTCTATCAGGGCGTGCGCAATCTGATTGGctc                                                >  8:103392/1‑124 (MQ=255)                                                                                                          ccgccTGCTTTCCAAATCCCAAGCAGAGCTGCAGGCATTTCCATTGACCGGCCCATCATTACAGCAACGATATCCCCAGATCCAACGCCTTTTTCTATCAGGGCGTGCGCAATCTGATTGGCTCTTACTTGTAACTgtc                                 >  1:351258/1‑137 (MQ=255)                                                                                                             ccTGCTTTCCAAATCCCAAGCAGAGCTGCAGGCATTTCCATTGACCGGCCCATCATTACAGCAACGATATCCCCAGATCCAACGCCTTTTTCTATCAGGGCGTGCGCAATCTGATTGGCTCTTACTTGTAACTGGCGAt                              <  5:172257/139‑1 (MQ=255)                                                                                                                               aGCAGAGCTGCAGGCATTTCCATTGACCGGCCCATTATTACAGCAACGATATCCCCAGATCCAACGCCTTTTTCTATCAGGGCGTGCGCAATCTGATTGGCTCTTACTTGTAACTg                                   >  3:311450/1‑116 (MQ=255)                                                                                                                               aGCAGAGCTGCAGGCATTTCCATTGACCGGCCCATTATTACAGCAACGATATCCCCAGATCCAACGCCTTTTTCTATCAGGGCGTGCGCAATCTGATTGGCTCTTACTTGTAACTg                                   <  4:311450/116‑1 (MQ=255)                                                                                                                                    agCTGCAGGCATTTCCATTGACCGGCCCATCATTACAGCAACGATATCCCCAGATCCAACGCCTTTTTCTATCAGGGCGTGCGCAATCTGATTGGCTCTTACTTGTAACTGGCGATAGGTCCAGCACTCGTTTCCCAtt       >  6:181414/1‑139 (MQ=255)                                                                                                                                         cAGGCATTTCCATTGACCGGCCCATCATTACAGCAACGATATCCCCAGATCCAACGCCTTTTTCTAGCAGGGCGTGCGCAAGCTGATGGGCTCTTACTTGTAACTGGCGATAGGTCCAGCACTCGTTTCCCATTTTCaa  >  2:290509/1‑139 (MQ=255)                                                                                                                                          |                                                                                                                                          AAGGAAATAAGGTCTTCTTCTATCAGCAATTGAGCCGCTTGACTGTCCTTCAGAAGAAAAGAAAGACGTTCTGCTGGAAAATGTGGATCAAGCGGCATATAAGCGCCGCCTGCTTTCCAAATCCCAAGCAGAGCTGCAGGCATTTCCATTGACCGGCCCATCATTACAGCAACGATATCCCCAGATCCAACGCCTTTTTCTATCAGGGCGTGCGCAATCTGATTGGCTCTTACTTGTAACTGGCGATAGGTCCAGCACTCGTTTCCCATTTTCAA  >  BS168_reference_genome/1988512‑1988786

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑