Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	BS168_reference_genome	1,317,158	(T)5→6	coding (257/258 nt)	yjpA ←	putative enzyme

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	BS168_reference_genome	1,317,153	1	.	T	100.0%	216.9 / NA	63	intergenic (+24/+4)	phrA/yjpA	secreted inhibitor of the activity of phosphatase RapA (quorum sensing)/putative enzyme
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base T (30/33);  total (30/33)

GGGTTCAGCTTTACTCAGGTGATGGTTCATGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAGGG‑‑C‑TTTTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGATTTCCGCTGCCTCTTCCAGTGAATCTGCTTCGATGACATACGCT  >  BS168_reference_genome/1317037‑1317274                                                                                                                        |                                                                                                                          gggTTCAGCTTTACTCAGGTGATGGTTCATGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAGGGGTCTTTTTTATTTCTTCAGCTTc                                                                                                        <  6:220420/139‑1 (MQ=255)   gTTCAGCTTTACTCAGGTGATGGTTCATGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAGGGGTCTTTTTTATTTCTTCAGCTTCCa                                                                                                      <  6:121828/139‑1 (MQ=255)   gTTCAGCTTTACTCAGGTGATGGTTCATGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAGGGGTCTTTTTTATTTCTTCAGCTTCCa                                                                                                      <  8:215086/139‑1 (MQ=255)      cAGCTTTACTCAGGTGATGGTTCATGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTTCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAGGGGTCTTTTTTATTTCTTCAGCTTCCATTc                                                                                                   >  3:133396/1‑139 (MQ=255)            tACTCAGGTGATGGTTCATGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAGGGGTCTTTTTTATTTCTTCAGCTTCCATTCTTTTAt                                                                                             >  7:213467/1‑139 (MQ=255)             aCTCAGGTGATGGTTCATGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAGGGGTCTTTTTTATTTCTTCAGCTTCCATTCTTTTATc                                                                                            >  5:16902/1‑139 (MQ=255)                 aGGTGATGGTTCCTGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAGGGGTCTTTTTTATTTCTTCAGCTTCCATTCTTTTATCGt                                                                                          <  3:247339/137‑1 (MQ=255)                 aGGTGATGGTTCATGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAGGGGTCTTTTTTATTTCTTCAGCTTCCATTCTTTTATCGt                                                                                          <  4:133396/137‑1 (MQ=255)                 aGGTGATGGTTCATGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAGGGGTCTTTTTTATTTCTTCAGCTTCCATTCTTTTATCGt                                                                                          <  1:309316/137‑1 (MQ=255)                  ggTGATGGTTCATGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAGGGGTCTTTTTTATTTCTTCAGCTTCCATTCTTTTATCGTCAg                                                                                       <  5:314410/139‑1 (MQ=255)                   gTGATGGTTCATGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAGGGGTCTTTTTTATTTGTTCAGCTTCCATTGTTTTATCGTAAGc                                                                                      >  1:355392/1‑139 (MQ=255)                   gTGATGGTTCATGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAGGGGTCTTTTTTATTTCTTCAGCTTCCATTCTTTTATCGTCa                                                                                        >  2:348782/1‑137 (MQ=255)                   gTGATGGTTCATGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAGGGGTCTTTTTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGc                                                                                      >  5:264124/1‑139 (MQ=255)                   gTGATGGTTCATGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAGGGGTCTTTTTTATTTCTTCAGCTTCCATTCTTTTATCGTAAGc                                                                                      >  7:139339/1‑139 (MQ=255)                      aTGGTTCATGCAGGTGAAACATCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAGGGGTCTTTTTTATTTCTTCATCTTCCATTCTTTTATCGTCAGCTc                                                                                    <  7:186877/138‑1 (MQ=255)                      aTGGTTCATGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAGGGGTCTTTTTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTc                                                                                    >  5:16213/1‑138 (MQ=255)                      aTGGTTCATGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAGGGGTCTTTTTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTc                                                                                    <  3:104664/138‑1 (MQ=255)                       tGGTTCATGCAGGGGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAGGGGTCTTTTTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCag                                                                                  <  6:277540/139‑1 (MQ=255)                           tCATGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAGGGGTCTTTTTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCag                                                                                  >  6:327713/1‑135 (MQ=255)                             aTGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGTCACGCAATCAAACATGATGAATAAAAAAAGACCCTTAGGGGTCTTTTTTATTTCTTCAGCTTCCATTATTTTATCGTCAGCTAAGAAGAt                                                                             >  7:145673/1‑138 (MQ=255)                             aTGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAGGGGTCTTTTTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATc                                                                            >  6:84065/1‑139 (MQ=255)                             aTGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAGGGGTCTTTTTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATc                                                                            <  3:330982/139‑1 (MQ=255)                                cAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAGGGGTCTTTTTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCAc                                                                         <  8:97137/139‑1 (MQ=255)                                   gTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAGGGGTCTTTTTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTg                                                                      >  5:113561/1‑139 (MQ=255)                                     gAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAGGGGTCTTTTTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGcc                                                                    <  6:363765/139‑1 (MQ=255)                                      aaaCAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAGGGGTCTTTTTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGcc                                                                    <  4:134503/138‑1 (MQ=255)                                      aaaCAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAGGGGTCTTTTTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGcc                                                                    <  7:188482/138‑1 (MQ=255)                                        aCAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAGGGGTCTTTTTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGccac                                                                  >  8:367889/1‑138 (MQ=255)                                          aGCAAACACAGAATGGAAAACATTTCATATTGCGGCAAGCAATCAAACATGATGCATAAAAAAAGACCCTTAGGGGTCTTTTTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAg                                                               <  2:147714/139‑1 (MQ=255)                                             aaaCACAGAAGGGAACACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAGGGGTCTTTTTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCgg                                                            <  6:16213/139‑1 (MQ=255)                                             aaaCACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAGGGGTCTTTTTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCgg                                                            <  6:264124/139‑1 (MQ=255)                                                    gAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAGGGGTCTTTTTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCgg                                                            <  7:29962/132‑1 (MQ=255)                                                    gAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAGGGGTCTTTTTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCgg                                                            >  8:29962/1‑132 (MQ=255)                                                    gAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAGGGGTCTTTTTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCa                                                     >  1:219501/1‑139 (MQ=255)                                                    gAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAGGGGTCTTTTTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCa                                                     >  1:221055/1‑139 (MQ=255)                                                       gggAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAGGGGTCTTTTTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATgg                                                  <  6:16902/139‑1 (MQ=255)                                                           aaaCATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAGGGGTCTTTTTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGa                                              >  4:189686/1‑139 (MQ=255)                                                           aaaCATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCATTAGGGGTCTTTTTTATTTCTTCAGCTTCCATTCTTTTATCTTCCGCTCAGAAGATCCACTTTCCACCAGCTCATACGCATGCCCg                                               >  6:174943/1‑138 (MQ=255)                                                                  tCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAGGGGTCTTTTTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGAGTTCCGc                                       >  2:80717/1‑139 (MQ=255)                                                                    atatTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAGGGGTCTTTTTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGATTTCCGCt                                      <  1:348782/138‑1 (MQ=255)                                                                    atatTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAGGGGTCTTTTTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGATTTCCGCt                                      <  2:19929/138‑1 (MQ=255)                                                                        tGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAGGGGTCTTTTTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCCGAAGATCCACTTGCCACCAGCGGATCCGCAAGGGCGATTTGCGGTGGctc                                 >  5:8762/1‑139 (MQ=255)                                                                        tGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAGGGGTCTTTTTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGATTTCCGCTGCctc                                 >  3:17749/1‑139 (MQ=255)                                                                          cGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAGGGGTCTTTTTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCCCCAGCGGATCCGCATGGCCGATTTACGCTGCCACtt                               >  7:251235/1‑139 (MQ=255)                                                                          cGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAGGGGTCTTTTTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGATTTCCGCTGCctct                                <  6:47773/138‑1 (MQ=255)                                                                            gCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAGGGGTCTTTTTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGATTTCCGCTGCCTCTTc                              >  3:198840/1‑138 (MQ=255)                                                                               cGCAATCAAACATGATGCATAAAAAAAGACCCTTAGGGGTCTTTTTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGATTTCCGCTGCCTCTTCCAGt                          >  3:217237/1‑139 (MQ=255)                                                                                           tgatgCATAAAAAAAGACCCTTAGGGGTCTTTTTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGATTTCCGCTGCCTCTTCCAGTGAATCTGCTTCg              <  1:116519/139‑1 (MQ=255)                                                                                           tgatgCATAAAAAAAGACCCTTAGGGGTCTTTTTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGATTTCCGCTGCCTCTTCCAGTGAATCTGCTTCg              <  5:84065/139‑1 (MQ=255)                                                                                            gatgCATAAAAAAAGACCCTTAGGGGTCTTTTTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGATTTCCGCTGCCTCTTCCAGTGAAt                      >  2:238037/1‑130 (MQ=255)                                                                                            gatgCATAAAAAAAGACCCTTAGGGGTCTTTTTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGATTTCCGCTGCCTCTTCCAGTGAAt                      <  1:238037/130‑1 (MQ=255)                                                                                             atgCATAAAAAAAGACCCTTAGGGGTCTTTTTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGATTTCCGCTGCCTCTTCCAGTGAATCTGCTTCGAt            <  5:191056/139‑1 (MQ=255)                                                                                              tgCATAAAAAAAGACCCTTAGGGGTCTTTTTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGATTTCCGCTGCCTCTTCCAGTGAATCTGCTTCg              >  6:356719/1‑136 (MQ=255)                                                                                              tgCATAAAAAAAGACCCTTAGGGGTCTTTTTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGATTTCCGCTGCCTCTTCCAGTGAATCTGCTTCg              <  5:356719/136‑1 (MQ=255)                                                                                              tgCATAAAAAAAGACCCTTAGGGGTCTTTTTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGATTTCCGCTGCCTCTTCCAGTGAATCTGCTTCGAt            >  4:181188/1‑138 (MQ=255)                                                                                              tgCATAAAAAAAGACCCTTAGGGGTCTTTTTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGATTTCCGCTGCCTCTTCCAGTGAATCTGCTTCGAt            >  6:156486/1‑138 (MQ=255)                                                                                                 aTAAAAAAAGACCCTTAGGGGTCTTTTTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCGGTTTTCCGCTGCCTCTTCCAGTGAATCTGCTTCGATGACa        <  8:145673/139‑1 (MQ=255)                                                                                                  tAAAAAAAGACCCTTAGGGGTCTTTTTTTTTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGATTTCCGCTGCCTCTTCCAGTGAATCTGCTTCGATGACa        >  4:242132/1‑138 (MQ=255)                                                                                                  tAAAAAAAGACCCTTAGGGGTCTTTTTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGATTTCCGCTGCCTCTTCCAGTGAATCTGCTTCGTTGACa        >  8:263263/1‑138 (MQ=255)                                                                                                      aaaaGACCCTTAGGGGTCTTTTTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGATTTCCGCTGCCTCTTCCAGTGAATCTGCTTCGATGACATACGc   <  3:242132/139‑1 (MQ=255)                                                                                                      aaaaGACCCTTAGGGGTCTTTTTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGATTTCCGCTGCCTCTTCCAGTGAATCTGCTTCGATGACATACGc   <  2:3786/139‑1 (MQ=255)                                                                                                       aaaGACCCTTAGGGGTCTTTTTTATTTCTTCAGCTTCCATTCTTTTATCGTCCGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGATTTCCGCTGCCTCTTCCAGTGAATCTGCTTCGATGACATACGCt  <  3:346979/139‑1 (MQ=255)                                                                                                       aaaGACCCTTAGGGGTCTTTTTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGATTTCCGCTGCCTCTTCCAGTGAATCTGCTTCGATGACATACGCt  <  3:189686/139‑1 (MQ=255)                                                                                                                        |                                                                                                                          GGGTTCAGCTTTACTCAGGTGATGGTTCATGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAGGG‑‑C‑TTTTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGATTTCCGCTGCCTCTTCCAGTGAATCTGCTTCGATGACATACGCT  >  BS168_reference_genome/1317037‑1317274

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑