| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | BS168_reference_genome | 2,387,885 | (A)7→8 | coding (704/735 nt) | yphF ← | putative lipoprotein of unknown function |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | BS168_reference_genome | 2,387,878 | 1 | . | A | 97.8% | 152.7 / ‑3.9 | 46 | coding (711/735 nt) | yphF | putative lipoprotein of unknown function |
| Reads supporting (aligned to +/- strand): ref base . (1/0); new base A (25/20); total (26/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ATGTGTATGACGTTGTCCTATTTGAATATGACATCTTTAGAAAATTTCTCATCCTCAATCTATTAATATAAAAAAACCTTTCTCCCTATTTGTATGCAAGGGAGAAAGGTTCATTCCTTTTTCATATCATTTTCT‑‑AA‑AAAAACAGGTTCCTGTTTGTCATTTACCGTATATTTTACAGAAAACGCGGGGACAAAGGGGGTTTCATGCCAAATCATATCCTGGATTTCAGTCCCGGGTTTAAACGTTTTTTTGCTTTTTTTGAGGCAA > BS168_reference_genome/2387744‑2388010 | atGTGTATGACGTTGTCCTATTTGAATATGACATCTTTAGAAAATTTCTCATCCTCAATCTATTAATATAAAAAAACCTTTCTCCCTATTTGTAGGCAAGGGAGAAAGGTTCATTCCTTTTTCATATCATTTTCT‑‑aa‑a > 3:154775/1‑138 (MQ=255) tATGACGTTGTCCTATTTGAATATGACATCTTTAGAAAATTTCTCATCCTCAATCTATTAATATAAAAAAACCTTTCTCCCTATTTGTATGCAAGGGAGAAAGGTTCATTCCTTTTTCATATCATTTTCT‑‑aa‑aaaaaa > 1:171180/1‑137 (MQ=255) tATGACGTTGTCCTATTTGAATATGACATCTTTAGAAAATTTCTCATCCTCAATCTATTAATATAAAAAAACCTTTCTCCCTATTTGTATGCAAGGGAGAAAGGTTCATTCCTTTTTCATATCATTTTCT‑‑aa‑aaaaaa > 5:197267/1‑137 (MQ=255) tATGACGTTGTCCTATTTGAATATGACATCTTTAGAAAATTTCTCATCCTCAATCTATTAATATAAAAAAACCTTTCTCCCTATTTGTATGCAAGGGAGAAAGGTTCATTCCTTTTTCATATCATTTTCT‑‑aa‑aaaaaa > 4:136807/1‑137 (MQ=255) tATGACGTTGTCCTATTTGAATATGACATCTTTAGAAAATTTCTCATCCTCAATCTATTAATATAAAAAAACCTTTCTCCCTATTTGTATGCAAGGGAGAAAGGTTCATTCCTTTTTCATATCATTTTCT‑‑aa‑aaaaaa > 3:106035/1‑137 (MQ=255) tGACGTTGTCCTATTTGAATATGACATCTTTAGAAAATTTCTCATCCTCAATCTATTAATATAAAAAAACCTTTCTCCCTATTTGTATGCTAGGGAGAAAGGTTCTTTCCTTTTTCTTATCATTTTCT‑‑AACAAAAACAg > 2:367403/1‑139 (MQ=255) tGACGTTGTCCTATTTGAATATGACATCTTTAGAAAATTTCTCATCCTCAATCTATTAATATAAAAAAACCTTTCTCCCTATTTGTATGCAAGGGAGAAAGGTTCATTCCTTTTTCATATCATTTTCT‑‑aa‑aaaaaacag > 3:247415/1‑135 (MQ=255) tGACGTTGTCCTATTTGAATATGACATCTTTAGAAAATTTCTCATCCTCAATCTATTAATATAAAAAAACCTTTCTCCCTATTTGTATGCAAGGGAGAAAGGTTCATTCCTTTTTCATATCATTTTCT‑‑aa‑aaaaaacag > 5:73977/1‑135 (MQ=255) tGACGTTGTCCTATTTGAATATGACATCTTTAGAAAATTTCTCATCCTCAATCTATTAATATAAAAAAACCTTTCTCCCTATTTGTATGCAAGGGAGAAAGGTTCATTCCTTTTTCATATCATTTTCT‑‑aa‑aaaaaacag > 5:313628/1‑135 (MQ=255) tGACGTTGTCCTATTTGAATATGACATCTTTAGAAAATTTCTCATCCTCAATCTATTAATATAAAAAAACCTTTCTCCCTATTTGTATGCAAGGGAGAAAGGTTCATTCCTTTTTCATATCATTTTCT‑‑aa‑aaaaaacag > 6:357400/1‑135 (MQ=255) tGACGTTGTCCTATTTGAATATGACATCTTTAGAAAATTTCTCATCCTCAATCTATTAATATAAAAAAACCTTTCTCCCTATTTGTATGCAAGGGAGAAAGGTTCATTCCTTTTTCATATCATTTTCT‑‑aa‑aaaaaacag > 3:15891/1‑135 (MQ=255) tGACGTTGTCCTATTTGAATATGACATCTTTAGAAAATTTCTCATACTCATTCTATTAATATAAAAAAACCTTTCTCCCTATATGTATGCAAGGGAGAAAGGTTCATTCCTTTTTCATATCATTTTCT‑‑aa‑aaaaaacag > 1:223653/1‑135 (MQ=255) gTTGTCCTATTTGAATATGACATCTTTAGAAAATTTCTCATCCTCAATCTATTAATATAAAAAAACCTTTCTCCCTATTTGTATGCAAGGGAGAAAGGTTCATTCCTTGTTCATATCATTTTCTA‑AA‑AAAAACAGGTTc > 8:24023/1‑139 (MQ=255) tCCTATTTGAATATGACATCTTTAGAAAATTTCTCATCCTCAATCTATTAATATAAAAAAACCTTTCTCCCTATTTGTATGCAAGGGAGAAAGGTTCATTCCTTTTTCATATCATTTTCTA‑AA‑AAAAACAGGTTCCTGt < 7:98991/139‑1 (MQ=255) tCCTATTTGAATATGACATCTTTAGAAAATTTCTCATCATCAATCTATTAATATAAAAAAACCTTTCTCCCTATTTGTATGCAAGGGAGAAAGGTTCATTCCTTTTTCATATCATTTTCTA‑AA‑AAAAACAGGTTCCTGt < 2:186862/139‑1 (MQ=255) tttGAATATGACATCTTTAGAAAATTTCTCATCCTCAATCTATTAATATAAAAAAACCTTTCTCCCTATTTGTATGCAAGGGAGAAAGGTTCATTCCTTTTTCATATCATTTTCTA‑AA‑AAAAACAGGTTCCTGTTTGt > 8:230360/1‑138 (MQ=255) tttGAATATGACATCTTTAGAAAATTTCTCATCCTCAATCTATTAATATAAAAAAACCTTTCTCCCTATTTGTATGCAAGGGAGAAAGGTTCATTCCTTTTTCATATCATTTTCTA‑AA‑AAAAACAGGTTCCTGTTTGTc > 3:190845/1‑139 (MQ=255) tttGAATATGACATCTTTAGAAAATTTCTCATCCTCAATCTATTAATATAAAAAAACCTTTCTCCCTATTTGTATGCAAGGGAGAAAGGTTCATTCCTTTTTCATATCATTTTCTA‑AA‑AAAAACAGGTTCATGTTTGt > 2:95790/1‑138 (MQ=255) gAATATGACATCATTAGAAAATATCTCATCCTCAATCTATTAATATAAAAAAACCTTTCTCCCTATTTGTATGCAAGGGAGAAAGGTTCATTCCTTTTTCATATCATTTTCTA‑AA‑AAAAACAGGTTCCTGTTTGTCAt < 6:274538/138‑1 (MQ=255) tGACATCTTTAGAAAATTTCTCATCCTCAATCTATTAATATAAAAAAACCTTTCTCCCTATTTGTATGCAAGGGAGAAAGGTTCATTCCTTTTTCATATCATTTTCTA‑AA‑AAAAACAGGTTCCTGTTTGTCATTTAcc < 8:122397/138‑1 (MQ=255) tGACATCTTTAGAAAATTTCTCATCCTCAATCTATTAATATAAAAAAACCTTTCTCCCTATTTGTATGCAAGGGAGAAAGGTTCATTCCTTTTTCATATCATTTTCTA‑AA‑AAAAACAGGTTCCTGTTTGTCATTTAcc < 8:367358/138‑1 (MQ=255) aCATCTTTAGAAAATTTCTCATCCTCAATCTATTAATATAAAAAAACCTTTCTCCCTATTTGTATGCAAGGGAGAAAGGTTCATTCCTTTTTCATATCATTTTCTAAAA‑AAAAACAGGTTCCTGTTTGTCATTTACCGt < 4:106035/139‑1 (MQ=255) cTTTAGAAAATTTCTCATCCTCAATCTATTAATATAAAAAAACCTTTCTCCCTATTTGTATGCAAGGGAGAAAGGTTCATTCCTTTTTCATATCATTTTCTA‑AA‑AAAAACAGGTTCCTGTTTGTCATTTACCGTATAtt > 3:204220/1‑139 (MQ=255) aTCCTCAATCTATTACTATAAAAAAACCTTTCTCCCTATTTGTATGCAAGGGAGAAAGGTTCATTCCTTTTTCATATCATTTTCTA‑AA‑AAAAACAGGTTCCTGTTTGTCATTTACCGTATATTTTACAGAAAAcgc < 8:230988/136‑1 (MQ=255) aTCCTCAATCTATTAATATAAAAAAACCTTTCTCCCTATTTGTATGCAAGGGAGAAAGGTTCATTCCTTTTTCATATCATTTTCTA‑AA‑AAAAACAGGTTCCTGTTTGTCATTTACCGTATATTTTACAGAAAAcgcg < 6:313628/137‑1 (MQ=255) aTCCTCAATCTATTAATATAAAAAAACCTTTCTCCCTATTTGTATGCAAGGGAGAAAGGTTCATTCCTTTTTCATATCATTTTCTA‑AA‑AAAAACAGGTTCCTGTTTGTCATTTACCGTATATTTTACAGAAAAcgc < 7:230360/136‑1 (MQ=255) aTCCTCAATCTATTAATATAAAAAAACCTTTCTCCCTATTTGTATGCAAGGGAGAAAGGTTCATTCCTTTTTCATATCATTTTCTA‑AA‑AAAAACAGGTTCCTGTTTGTCATTTACCGTATATTTTACAGAAAAcgc < 6:29717/136‑1 (MQ=255) tAATATAAAAAAACCTTTCTCCCTATTTGTATGCAAGGGAGAAAGGTTCATTCCTTTTTCATATCATTTTCTA‑AA‑AAAAACAGGTTCCTGTTTGTCATTTACAGTATATTTTACAGAAAACGCGGGGACAAAGGGGGtt > 4:178708/1‑139 (MQ=255) tataAAAAAACCTTTCTCCCTATTTGTATGCAAGGGAGAAAGGTTCATTCCTTTTTCATATCATTTTCTA‑AA‑AAAAACAGGTTCCTGttt > 2:115303/1‑90 (MQ=255) tataAAAAAACCTTTCTCCCTATTTGTATGCAAGGGAGAAAGGTTCATTCCTTTTTCATATCATTTTCTA‑AA‑AAAAACAGGTTCCTGttt < 1:115303/90‑1 (MQ=255) aaaaaaCCTTTCTCCCTATTTGTATGCAAGGGAGAAAGGTTCATTCCTTTTTCATATCATTTTCTA‑AA‑AAAAACAGGTTCCTGTTTGTCATTTACCGTATATTTTACAGAAAACGCGGGGACAAAGGGGGTTTCATGcc < 4:247415/139‑1 (MQ=255) aaaaaaCCTTTCTCCCTATTTGTATGCAAGGGAGAAAGGTTCATTCCTTTTTCATATCATTTTCTA‑AA‑AAAAACAGGTTCCTGTTTGTCATTTACCGTATATTTTACAGAAAACGCGGGGACAAAGGGGGTTTCATGcc < 3:178708/139‑1 (MQ=255) aaaaaaCCTTTCTCCCTATTTGTATGCAAGGGAGAAAGGTTCATTCCTTTTTCATATCATTTTCTA‑AA‑AAAAACAGGTTCCTGTTTGTCATTTACCGTATATTTTACAGAAAACGCGGGGACAAAGGGGGTTTCATGc > 3:352125/1‑138 (MQ=255) aaaaaaCCTTTCTCCCTATTTGTATGCAAGGGAGAAAGGTTCATTCCTTTTTCATATCATTTTCTA‑AA‑AAAAACAGGTTCCTATTAGTCATTTACCGTATATTTTAAAGAAAACACGATCACAAAGGGTATTTCAttc > 6:25010/1‑136 (MQ=255) aaaaCCTTTCTCCCTATTTGTATGCAAGGGAGAAAGGTTCATTCCTTTTTCATATCATTTTCTA‑AA‑AAAAACAGGTTCCTGTTTGTCATTTACCTTATATTTTAAAGAAAACGCGGTGACAATGTGGGTTTCATGCCa > 7:141642/1‑138 (MQ=255) ccTATTTGTATGCAAGGGAGAAAGGTTCATTCCTTTTTCATATCATTTTCTA‑AA‑AAAAACAGGTTCCTGTTTGTCATTTACCGTATATTTTACAGAAAACGCGGGGACAAAGGGGGTTTCATGCCAAATCATATCCTg < 4:76109/138‑1 (MQ=255) ccTATTTGTATGCAAGGGAGAAAGGTTCATTACTTTTTCATATCATTTTCTA‑AA‑AAAAACAGGTTCCTGTTTGTCATTTACCGTATATTTTACAGAAAACGCGGGGACAAAGGGGGTTTCATGCCAAATCATATCCTg < 2:171180/138‑1 (MQ=255) aTTTGTATGCAAGGGAGAAAGGTTCATTCCTTTTTCATATCATTTTCTA‑AA‑AAAAACAGGTTCCTGTTTGTCATTTACCGTATATTTTACAGAAAAcgcg < 1:362486/100‑1 (MQ=255) aTTTGTATGCAAGGGAGAAAGGTTCATTCCTTTTTCATATCATTTTCTA‑AA‑AAAAACAGGTTCCTGTTTGTCATTTACCGTATATTTTACAGAAAAcgcg > 2:362486/1‑100 (MQ=255) tATGCAAGGGAGAAAGGTTCATTCCTTTTTCATATCATTTTCTA‑AA‑AAAAACAGGTTCCTGTTTGTCATTTACCGTATATTTTACAGAAAACGCGGGGACAAAGGGGGTTTCTTGCCAAATCATATCCTGGATTTCAGt > 6:285723/1‑139 (MQ=255) tGCAAGGGAGAAAGGTTCATTCCTTTTTCATATCATTTTCTA‑AA‑AAAAACAGGTTCCTGTTTGTCATTTACCGTATATTTTACAGAAAACGCGGGGACAAAGGGGGTTTCATGCCAAATCATATCCTGGATTTCAGTcc > 8:88242/1‑139 (MQ=255) tGCAAGGGAGAAAGGTTCATTCCTTTTTCATATCATTTTCTA‑AA‑AAAAACAGGTTCCTGTTTGTCATTTACCGTATATTTTACAGAAAACGCGGGGACAAAGGGGGTTTCATGCCAAATCATATCCTGGATTTCAGTcc > 1:331541/1‑139 (MQ=255) tGCAAGGGAGAAAGGTTCATTCCTTTTTCATATCATTTTCTA‑AA‑AAAAACAGGTTCCTGTTTGTCATTTACCGTATATTTTACAGAAAACGCGGGGACAAAGGGGGTTTCATGCCAAATCATATCCTGGATTTCAGTcc > 8:275753/1‑139 (MQ=255) tGCAAGGGAGAAAGGTTCATTCCTTTTTCATATCATTTTCTA‑AA‑AAAAACAGGTTCCTGTTTGTCATTTACCGTATATTTTACAGAAAACGCGGGGACAAAGGGGGTTTCATGCCAAATCATATCCTGGATTTCAGTcc > 3:288996/1‑139 (MQ=255) tGCAAGGGAGAAAGGTTCATTCCTTTTTCATATCATTTTCTA‑AA‑AAAAACAGGTTCCTGTTTGTCATTTACCGTATATTTTACAGAAAACGCGGGGACAAAGGGGGTTTCATGCCAAATCATATCCTGGATTTCAGTcc > 7:154218/1‑139 (MQ=255) tGCAAGGGAGAAAGGTTCATTCCTTTTTCATATCATTTTCTA‑AA‑AAAAACAGGTTCCTGTTTGTCATTTACCGTATATTTTACAGAAAACGCGGGGACAAAGGGGGTTTCATGCCAAATCATATCCTGGATTTCAGTcc < 6:73977/139‑1 (MQ=255) tGCAAGGGAGAAAGGTTCATTCCTTTTTCATATCATTTTCTA‑AA‑AAAAACAGGTTCCTGTTTGTCATTTACAGTATATTTTACAGAAAACGCGGGGACAAAGGGGGTTTCATGCCAAATCATATCCTGGATTTCAGTca > 7:274421/1‑138 (MQ=255) agaAAGGTTCATTCCTTTTTCATATCATTTTCTA‑AA‑AAAAACAGGTTCCTGTTTGTCATTTACCTTATATTTTACAGAAAACGCGGGGACAAAGGGGGTTTCATGCCAAATCATATCCTGGATTTCAGTCCCGGGttt > 4:261409/1‑138 (MQ=255) ttCATTCCTTTTTCATATCATTTTCTA‑AA‑AAAAACAGGTTCCTGTTTGTCATTTACCGTATATTTTACTGAAAACGCGTGGACAAAGGGGGTTTCATGCCAAGTCATATCCTGGATTTCAGTCCAGGGTTTATCCGttt > 8:133269/1‑139 (MQ=255) ttCATTCCTTTTTCATATCATTTTCTA‑AA‑AAAAACAGGTTCCTGTTTGTCATTTACCGTATATTTTACAGAAAACGCGGGGACAAAGGGGGTTTCATGCCAAATCATATCCTGGATTTCAGTCCCGGGTTTAAACGttt > 4:94685/1‑139 (MQ=255) cATTCCTTTTTCATATCATTTTCTA‑AA‑AAAAACAGGTTCCTGTTTGTCATTTACCGTATATTTTACAGAAAACGCGGGGACAAAGGGGGttt > 5:57084/1‑92 (MQ=255) cATTCCTTTTTCATATCATTTTCTA‑AA‑AAAAACAGGTTCCTGTTTGTCATTTACCGTATATTTTACAGAAAACGCGGGGACAAAGGGGGttt < 6:57084/92‑1 (MQ=255) cATTCCTTTTTCATATCATTTTCTA‑AA‑AAAAACAGGTTCCTGTTTGTCATTTACCGTATATTTTACAGAAAACGCGGGGACAAAGGGGGTTTCATGCCAAATCATATCCTGGATTTCAGTCCCGGGTTTAAACGttttt > 5:277881/1‑139 (MQ=255) tttCATATCATTTTCTA‑AA‑AAAAACAGGTTCCTGTTTGTCATTTACCGTATATTTTACAGAAAACGCGGGGACAAAGGGGGTTTCATGCCAAATCATATCCTGGATTTCAGTCCCGGGTTTAAACGTTTTTTTGCtttt > 3:228866/1‑139 (MQ=255) tttCATATCATTTTCTA‑AA‑AAAAACAGGTTCCTGTTTGTCATTTACCGTATATTTTACAGAAAACGCGGGGACAAAGGGGGTTTCATGCCAAATCATATCCTGGATTTCAGTCCCGGGTTTAAACGTTTTTTTGCtttt > 4:296457/1‑139 (MQ=255) ttttCTA‑AA‑AAAAACAGGTTCCTGTTTGTCATTTACCGTATATTTTACAGAAAACGCGGGGACAAAGGGGGTTTCATGCCAAATCATATCCTGGATTTCAGTCCCGGGTTTAAACGTTTTTTTGCTTTTTTTGAGGCaa < 4:15891/139‑1 (MQ=255) ttttCTA‑AA‑AAAAACAGGTTCCTGTTTGTCATTTACCGTATATTTTACAGAAAACGCGGGGACAAAGGGGGTTTCATGCCAAATCATATCCTGGATTTCAGTCCCGGGTTTAAACGTTTTTTTGCTTTTTTTGAGGCaa < 6:277881/139‑1 (MQ=255) | ATGTGTATGACGTTGTCCTATTTGAATATGACATCTTTAGAAAATTTCTCATCCTCAATCTATTAATATAAAAAAACCTTTCTCCCTATTTGTATGCAAGGGAGAAAGGTTCATTCCTTTTTCATATCATTTTCT‑‑AA‑AAAAACAGGTTCCTGTTTGTCATTTACCGTATATTTTACAGAAAACGCGGGGACAAAGGGGGTTTCATGCCAAATCATATCCTGGATTTCAGTCCCGGGTTTAAACGTTTTTTTGCTTTTTTTGAGGCAA > BS168_reference_genome/2387744‑2388010 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |