Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	BS168_reference_genome	1,474,890	C→T	L45L (TTG→TTA)	kre ←	regulator of transcription factor ComK function via modulation of mRNA stability

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	BS168_reference_genome	1,474,890	0	C	T	100.0%	209.1 / NA	57	L45L (TTG→TTA)	kre	regulator of transcription factor ComK function via modulation of mRNA stability
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (26/31);  total (26/31)

AGGCATGTGAAAATAGTAGTCTCCGATTGAGATAAGCACGTCCGATTGCTGTTGGCTGAACCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGCAAAGCCCGTTTTTTCAGCAGATATAGGTATTTAGGGTTGGGGGCGGTTTTTGCATGGCGGTTCACAGTGTAAACTGCTTTTGAAAGATTTTCTACGGTTGGCTGCAGATCTTTCGTATATGCATGGTCGTC  >  BS168_reference_genome/1474767‑1475021                                                                                                                            |                                                                                                                                    aGGCATGTGAAAATAGTAGTCTCCGATTGATATAAGCACGTCCGATTGCTGTTGGCTGAACATTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGATGTTGTAAAGCGCGtatttt                                                                                                                       >  4:274362/1‑138 (MQ=255)   tcATGTGAAAATAGTAGTCTCCGATTGAGATAAGCACGTCCGATTGCTGTTGGCTGAACCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTcag                                                                                                                    <  5:31865/138‑1 (MQ=255)   gCATGTGAAAATAGTGGTCTCCGATTGAGATAAGCACGTCCGATTGCTGTTGGCTGAACCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTcag                                                                                                                    <  7:290467/139‑1 (MQ=255)   gCATGTGAAAATAGTAGTCTCCGATTGAGATAAGCACGTCCGATTGCTGTTGGCTGAACCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTcag                                                                                                                    <  1:172573/139‑1 (MQ=255)    cATGTGAAAATAGTAGTCTCCGATTGAGATAAGCACGTCCGATTGCTGTTGGCTGAACCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTcagc                                                                                                                   <  7:125328/139‑1 (MQ=255)    cATGTGAAAATAGTAGTCTCCGATTGAGATAAGCACGTCCGATTGCTGTTGGCTGAACCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTcagc                                                                                                                   <  3:164624/139‑1 (MQ=255)    cATGTGAAAATAGTAGTCTCCGATTGAGATAAGCACGTCCGATTGCTGTTGGCTGAACCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTcagc                                                                                                                   <  5:39971/139‑1 (MQ=255)      tgtgAAAATAGTAGTCTCCGATTGAGATAAGCACGTCCGATTGCTGTTGGCTGAACCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTcag                                                                                                                    >  4:38146/1‑136 (MQ=255)          aaaaTAGTAGTCTCCGATTGAGATAAGCACGTCCGATTGCTGTTGGCTGAACCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGatat                                                                                                             <  7:76967/139‑1 (MQ=255)           aaaTAGTAGTCTCCGATTGAGATAAGCACGTCCGATTGCTGTTGGCTGAACCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGAtata                                                                                                            <  3:38146/139‑1 (MQ=255)           aaaTAGTAGTCTCCGATTGAGATAAGCACGTCCGATTGCTGTTGGCTGAACCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGAtata                                                                                                            <  3:206878/139‑1 (MQ=255)           aaaTAGTAGTCTCCGATTGAGATAAGCACGTCCGATTGCTGTTGGCTGAACCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGAtata                                                                                                            <  8:194618/139‑1 (MQ=255)                 tagtCTCCGATTGAGATAAGCACGTCCGATTGCTGTTGGCTGAACCTTGGATTTTTTGAAAAATGACGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGATATAGGTAtt                                                                                                      >  2:40319/1‑139 (MQ=255)                 tagtCTCCGATTGAGATAAGCACGTCCGATTGCTGTTGGCTGAACCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGATATAGGTAtt                                                                                                      >  1:139711/1‑139 (MQ=255)                    tctcCGATTGAGATAAGCACGTCCGATTGCTGTTGGCTGAACCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGATATAGGTATTTAg                                                                                                   >  7:198359/1‑139 (MQ=255)                      tcCGATTGAGATAAGCACGTCCGATTGCTGTTGGCTGAACCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGATATAg                                                                                                           >  1:62716/1‑129 (MQ=255)                      tcCGATTGAGATAAGCACGTCCGATTGCTGTTGGCTGAACCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGATATAg                                                                                                           <  2:62716/129‑1 (MQ=255)                      tcCGATTGAGATAAGCACGTCCGATTGCTGTTGGCTGAACCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGATATAGGTATTTAggg                                                                                                 >  5:285683/1‑139 (MQ=255)                              agaTAAGCACGTCCGATTGCTGTTGGCTGAACCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGATATAGGTATTTAGGGTTGGGGGc                                                                                         >  7:40305/1‑139 (MQ=255)                                     cACGTCCGATTGCTGTTGGCTGAACCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGATATAGGTATTTAGGGTTGGGGGCGGttttt                                                                                  >  5:139655/1‑139 (MQ=255)                                        gTCCGATTGCTGTTGGCTGAACCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGAtata                                                                                                            >  3:203479/1‑110 (MQ=255)                                        gTCCGATTGCTGTTGGCTGAACCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGAtata                                                                                                            <  4:203479/110‑1 (MQ=255)                                        gTCCGATTGCTGTTGGCTGAACCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGATATAGGTATTTAGGGTTGGGGGCGGTTTTTGCa                                                                               <  5:208716/139‑1 (MQ=255)                                        gTCCGATTGCTGTTGGCTGAACCTTGGATTTTTTGAAAAATGAAGCCAATTTTTATTTCGTTTACATTATTTTCCAACATTTTGTAAAGCCCTTTTTTTCAGAATTTTTAGGTTTTTTGGGTTGGGGGCGGTTTTTGCa                                                                               >  7:246030/1‑139 (MQ=255)                                          ccGATTGCTGTTGGCTGAACCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGATATAGGTATTTAGGGTTGGGGGCGGTTTTTGCATg                                                                             >  3:200650/1‑139 (MQ=255)                                                    ttGGCTTAACCTTGGATTTTTTGAAAAATGAAGCACTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTATTCAGGAGATATAGGTATTTAGGGTTGGGGGCGGTTTTTTCATGGCTGTTcac                                                                    <  2:242332/138‑1 (MQ=255)                                                    ttGGCTGAACCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGATATAGGTATTTAGGGTTGGGGGCGGTTTTTGCATGGCGGTTca                                                                     <  5:193448/137‑1 (MQ=255)                                                      ggCTGAACCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGATATAGGTATTTATGGTTGGGGGCGGGTTTTGCATGGCGGTTCACAgt                                                                 >  5:41414/1‑139 (MQ=255)                                                         tGAACCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGATATAGGTATTTAGGGTTGGGGGCGGTTTTTGCATGGCGGTTCACAgtgt                                                               >  2:86244/1‑138 (MQ=255)                                                           aaCCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGATATAGGTATTTAGGGTTGGGGGCGGTTTTTGCATGGCGGTTCACAGTGTaaa                                                            <  7:337160/139‑1 (MQ=255)                                                           aaCCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGATATAGGTATTTAGGGTTGGGGGCGGTTTTTGCATGGCGGTTCACAGTGTaaa                                                            >  8:73438/1‑139 (MQ=255)                                                           aaCCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGATTTTTCAGCAGATATAGGTATTTAGGGTTGGGTGCGGTTTTTGCATGGCGGTTCACAGTGTaa                                                             >  2:267938/1‑138 (MQ=255)                                                                tGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTcag                                                                                                                    >  2:89921/1‑78 (MQ=255)                                                                tGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTcag                                                                                                                    <  1:89921/78‑1 (MQ=255)                                                                tGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGATATAGGTATTTAGGGTTGGGGGCGGTTTTTGCa                                                                               <  1:338178/115‑1 (MQ=255)                                                                tGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGATATAGGTATTTAGGGTTGGGGGCGGTTTTTGCa                                                                               >  2:338178/1‑115 (MQ=255)                                                                          gAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGATATAGGTATTTAGGGTTGGGGGCGGTTTTTGCATGGCGGTTCACAGTGTAAACTGCTTTTGAAAGa                                              <  4:10248/138‑1 (MQ=255)                                                                                               tttcCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGATATAGGTATTTAGGGTTGGGGGCGGTTTTTGCATGGCGGTTCACAGTGTAAACTGCTTTTGAAAGATTTTCTACGGTTGGCTg                             >  3:326655/1‑134 (MQ=255)                                                                                                 tcCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGATATAGGTATTTAGGGTTGGGGGCGGTTTTTGCATGGCGGTTCACAGTGTAAACTGCTTTTGAAAGATTTTCTACGGTTGGCTGCAGATc                       <  5:65062/138‑1 (MQ=255)                                                                                                   cTTTACCTTCTTTGACCAGGTTTTGTAAAGCCCGTTTTTTCAGCAGATTTATGTATTTAGGGTTGGGGGCGGTTTTTGCATGGCGGATCACAGTGTAAACTGCTTTTGAAAGATTTTCTACGGTTGGCTGCAGATCtt                     <  1:267938/138‑1 (MQ=255)                                                                                                   cTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGATATAGGTATTTAGGGTTGGGGGCGGTTTTTGCATGGCGGTTCACAGTGTAAACTGCTTTTGAAAGATTTTCTACGGTTGGCTGCAGATCtt                     <  4:326655/138‑1 (MQ=255)                                                                                                   cTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGATATAGGTATTTAGGGTTGGGGGCGGTTTTTGCATGGCGGTTCACAGTGTAAACTGCTTTTGAAAGATTTTCTACGGTTGGCTGCAGATCtt                     <  5:75716/138‑1 (MQ=255)                                                                                                   cTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGATATAGGTATTTAGGGTTGGGGGCGGTTTTTGCATGGCGGTTCACAGTGTAAACTGCTTTTGAAAGATTTTCTACGGTTGGCTGCAGATCtt                     <  7:73438/138‑1 (MQ=255)                                                                                                      tACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGATATAGGTATTTAGGGTTGGGGGCGGTTTTTTCATGGCGGTTCACAGTGTAAACTGCTTTTGAAAGATTTTCTACGGtt                                  >  3:199624/1‑122 (MQ=255)                                                                                                      tACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGATATAGGTATTTAGGGTTGGGGGCGGTTTTTGCATGGCGGTTCACAGTGTAAACTGCTTTTGAAAGATTTTCTACGGtt                                  <  4:199624/122‑1 (MQ=255)                                                                                                      gagcttcttTGACAATCTTTTGTAAAGCACGTTTTTTCATCAGATATAGGTATTTAGTGTTGGGGGCGGTTTTTGCATGGCGGTTCAGAGTGTAAACTGCTTTTGAAAGATTTTCTACGGTTGGCTGCAGATCTTTCGt                 <  2:286030/136‑1 (MQ=255)                                                                                                       aCCTTCTTTGACAAGCTTTTGTAAAGGCCGTTTTTTCAGCAGATATAGGTATTTAGGGTTGGGGGCGGTTTTTGCCTGGCGGTTCACAGTGTAAACTGCTTTTGAAAGATTTTCTACGGTTGGCTGCAGATCTTTCGt                 <  8:198359/138‑1 (MQ=255)                                                                                                       aCCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGATATAGGTATTTAGGGTTGGGGGCGGTTTTTGCATGGCGGTTCACAGTGTAAACTGCTTTTGAAAGATTTTCTACGGTTGGCTGCAGATCTTTCGt                 <  6:285683/138‑1 (MQ=255)                                                                                                       aCCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGATATAGGTATTTAGGGGTGGGGGCGGTTTTTGCATGGCGGTTCACAGTGTAAACTGCTTTTGAAAGATTTTCTACGGTTGGCTGCAGATCTTTCGt                 <  3:173364/138‑1 (MQ=255)                                                                                                             tttGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGATATAGGTATTTAGGGTTGGGGGCGGTTTTTGCATGGCGGTTCACAGTGTAAACTGCTTTTGAAAGATTTTCTACGGTTGGCTGCAGATCTTTCGTATATGCa          >  2:242803/1‑139 (MQ=255)                                                                                                                gACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGATATAGGTATTTAGGGTTGGGGGCGGTTGTTGCATGGCGGTTCACAGTGTAAACTGCTTTTGAAAGATTTTCTACGGTTGGCTGCAGATCTTTCGTATATGCATg        <  8:274066/138‑1 (MQ=255)                                                                                                                  cAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGATATAGGTATTTAGGGTTGGGGTCGGTTTTTGCAAGGCGGTTCACAGTGTAAACAGCATTTGAAAGATTTTCTACGGTTGGCTGCAGATCTTTCGTATATGCATGgt      >  1:286030/1‑138 (MQ=255)                                                                                                                   aaGCTTTTGTAAAGCCCGTTTTTTCAGCAGATATAGGTATTTAGGGTTGGGGGCGGTTTTTGCTTGGCGGTTCCCAGTGTAAACTGCTTTTGAAAGATTTTCTACGGTTGGCTGCAGATCTTTCGTATATGCATGttcg    >  8:333397/1‑139 (MQ=255)                                                                                                                     gCTTTTGTAACGCCCGTTTTTTCAGCAGATATAGGTATTTAGGGTTGGGGGCGGTTTTTGCATGGCGGTTCACAGTGTGAACTGCTTTTGAAAGATTTTCTAGGGTTGGCTGCAGATCTTTCGTATATGCATGgtcgtc  >  2:257740/1‑139 (MQ=255)                                                                                                                     gCTTTTGTAAAGCCCGTTTTTTCAGCAGATATAGGGATTTAGGGTTGGGGGCGGTTTTTGCATGGCGGTTCACAGTGTAAACTGCTTTTGAAAGATTTTCTACGGTTGGCTGCAGATCTTTCGTATATGCATGgtcgtc  <  3:189919/139‑1 (MQ=255)                                                                                                                      cTTTTGTAAAGCCCGTTTTTTCAGCAGATATAGGTATTTAGGGTTGGGGGCGGTTTTTGCATGGCGGTTCACAGTGTAAACTGCTTTTGAAAGATTTTCTACGGTTGGCTGCAGATCTTTCGTATATGCATGgtcgtc  >  7:12755/1‑138 (MQ=255)                                                                                                                      cTTTTGTAAAGCCCGTTTTTTCAGCAGATATAGGTATTTAGGGTTGGGGGCGGTTTTTGCATGGCGGTTCACAGTGTAAACTGCTTTTGAAAGATTTTCTACGGTTGGCTGCAGATCTTTCGTATATGCATGgtcgtc  >  1:73706/1‑138 (MQ=255)                                                                                                                            |                                                                                                                                    AGGCATGTGAAAATAGTAGTCTCCGATTGAGATAAGCACGTCCGATTGCTGTTGGCTGAACCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGCAAAGCCCGTTTTTTCAGCAGATATAGGTATTTAGGGTTGGGGGCGGTTTTTGCATGGCGGTTCACAGTGTAAACTGCTTTTGAAAGATTTTCTACGGTTGGCTGCAGATCTTTCGTATATGCATGGTCGTC  >  BS168_reference_genome/1474767‑1475021

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑