Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	BS168_reference_genome	2,485,359	T→C	F150S (TTC→TCC)	yqjF →	conserved protein of unknown function

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	BS168_reference_genome	2,485,359	0	T	C	100.0%	226.0 / NA	59	F150S (TTC→TCC)	yqjF	conserved protein of unknown function
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (28/31);  total (28/31)

ATCATCGTCTGGCGGTCCTTGGCGCTCGCACATTCTTTCATCTGCCTTATTTTTACGCGGATATGAAGTCCGAAAAAAACGGTGATGCCATTGACTATGTCAGCAAGCGGAAAAATGACAAAGAAGCAGCATTCCACGCCGCATACCGGCCAATTTCAGCGCCTTTTACAGCAGAAAAGGATTCGCTCGATTACTGGCTGACAGAACGATACAGGCTCTACACCACTTATCGCAATAAGCTCTATTACGAAGACATCCATCAT  >  BS168_reference_genome/2485227‑2485489                                                                                                                                     |                                                                                                                                   ttcatcGTCGGGCGGTACTTGGCGCTCTCACATTCTTTCATCTGCATTATTTTTAAGAGTATATGACGTCCGAAAAAAACGGGGATGCCATTGACTATGTCAGAAAGCGGAAAAATGACAAAGACGCAGCAACCCAcgc                                                                                                                              <  2:55337/138‑1 (MQ=255)   catcGTCTGGCGGTCCTTGGCGCTCGCACATTCTTTCATCTGCCTTATTTTTACGCGGATATGAAGTCCGAAAAAAACGGTGATGCCATTGACTATGTCAGCAAGCGGAAAAATGACAAAGAAGCAGCATCCCAcgccg                                                                                                                            >  3:139841/1‑139 (MQ=255)      cGTCTGGCGGTCCTTGGCGCTCGCACATTCTTTCATCTGCCTTATTTTTACGCGGATATGAAGTCCGAAAAAAACGGTGATGCCATTGACTATGTCAGCAAGCGGAAAAATGACAAAGAAGCAGCATCCCACGCCGCa                                                                                                                          >  5:92444/1‑138 (MQ=255)                  cTTGGCGCTCGCACATTCTTTCATCTGCCTTATTTTTACGCGGATATGAAGTCCGAAAAAAACGGTGATGCCATTGACTATGTCAGCAAGCGGAAAAATGACAAAGAAGCAGCATCCCACGCCGCATACCGGCCAAttt                                                                                                             >  1:64775/1‑139 (MQ=255)                       cgcTCGCACATTCTTTCATCTGCCTTATTTTTACGCGGATATGAAGTCCGAAAAAAACGGGGATGCCATTGACTATGTCAGCAAGCGGAAAAATGACAAAGAAGCAGCATCCCACGCCGCATACCGGCCAATTTCAgcg                                                                                                        <  7:105849/139‑1 (MQ=255)                         cTCGCACATTCTTTCATCTGCCTTATTTTTACGCGGATATGAAGTCCGAAAAAAACGGAGATGCCATTGACTATGTCAGCAAGCGGAAAAATGACAAAGAAGCAGCATCCCACGCCGCATACCGGCCAATTTCAGCGcc                                                                                                      <  6:121629/139‑1 (MQ=255)                          tCGCACATTCTTTCATCTGCCTTATTTTTACGCGGATATGAAGTCCGAAAAAAACGGTGATGCCATTGACTATGTCAGCAAGCGGAAAAATGACAAAGAAGCAGCATCCCACGCCGCATACCGGCCAATTTCAGCGCCt                                                                                                     >  3:121806/1‑139 (MQ=255)                                 ttCTTTCATCTGCCTTATTTTTACGCGGATATGAAGTCCGAAAAAAACGGTGATGCCATTGACTATGTCAGCAAGCGGAAAAATGACAAAGAAGCAGCATCCCACGCCGCATACCGGCCAATTTCAGCGCCTTTTAcag                                                                                              <  6:92444/139‑1 (MQ=255)                                            gCCTTATTTTTACGCGGATATGAAGTCCGAAAAAAACGGTGATGCCATTGACTATGTCAGCAAGCGGAAAAATGACAAAGAAGCAGCATCCCAcg                                                                                                                               >  7:273239/1‑95 (MQ=255)                                            gCCTTATTTTTACGCGGATATGAAGTCCGAAAAAAACGGTGATGCCATTGACTATGTCAGCAAGCGGAAAAATGACAAAGAAGCAGCATCCCAcg                                                                                                                               <  8:273239/95‑1 (MQ=255)                                            gCCTTATTTTTACGCGGATATGAAGTCCGAAAAAAACGGTGATGCCATTGACTATGTCAGCAAGCGGAAAAATGACAAAGAAGCAGCATCCCACGCCGCATACCGGCCAATTTCAGCGCCTTTTACAGCAGAAAAGGAt                                                                                   >  4:338169/1‑139 (MQ=255)                                            gCCTTATTTTTACGCGGATATGAAGTCCGAAAAAAACGGTGATGCCATTGACTATGTCAGCAAGCGGAAAAATGACAAAGAAGCAGCATCCCACGCCGCATACCGGCCAATTTCAGCGCCTTTTACAGCAGAAAAGGAt                                                                                   >  3:172637/1‑139 (MQ=255)                                            gCCTTATTTTTACGCGGATATGAAGTCCGAAAAAAACGGTGATGCCATTGACTATGTCAGCAAGCGGAAAAATGACAAAGAAGCAGCATCCCACGCCGCATACCGGCCAATTTCAGCGCCTTTTACAGCAGAAAAGGAt                                                                                   >  1:319315/1‑139 (MQ=255)                                                  tttttACGCGGATATGAAGTCCGAAAAAAACGGTGATGCCATTGACTATGTCAGCAAGCGGAAAAATGACAAAGAAGCAGCATCCCACGCCGCATACCGGCCAATTTCAGCGCCTTTTACAGCAGAAAAGGATTCGCt                                                                              <  4:121806/138‑1 (MQ=255)                                                       aCGCGGATATGAAGTCCGAAAAAAACGGTGATGCCATTGACTATGTCAGCAAGCGGAAAAATGACAAAGAAGCAGCATCCCACGCCGCATACCGGCCAATTTCAGCGCCTTTTACAGCAGAAAAGGATTCGCt                                                                              <  8:32327/133‑1 (MQ=255)                                                       aCGCGGATATGAAGTCCGAAAAAAACGGTGATGCCATTGACTATGTCAGCAAGCGGAAAAATGACAAAGAAGCAGCATCCCACGCCGCATACCGGCCAATTTCAGCGCCTTTTACAGCAGAAAAGGATTCGCt                                                                              >  7:32327/1‑133 (MQ=255)                                                       aCGCGGATATGAAGTCCGAAAAAAACGGTGATGCCATTGACTATGTCAGCAAGCGGAAAAATGACAAAGAAGCAGCATCCCACGCCGCATACCGGCCAATTTCAGCGCCTTTTACAGCAGAAAAGGATTCGCTCGATTa                                                                        >  5:83359/1‑139 (MQ=255)                                                       aCGCGGATATGAAGTCCGAAAAAAACGGTGATGCCATTGACTATGTCAGCAAGCGGAAAAATGACAAAGAAGCAGCATCCCACGCCGCATACCGGCCAATTTCAGCGCCTTTTACAGCAGAAAAGGATTCGCTCGATTa                                                                        >  2:67334/1‑139 (MQ=255)                                                                tGAAGTCCGAAAAAAACGGTGATGCCATTGACTATGTCAGCAAGCGGAAAAATGACAAAGAAGCAGCATCCCACGCCGCATACCGGCCAATTTCAGCGCCTTTTACAGCAGAAAAGGATTCGCTCGATTACTGGCTGa                                                                <  3:181123/138‑1 (MQ=255)                                                                tGAAGTCCGAAAAAAACGGTGATGCCATTGACTATGTCAGCAAGCGGAAAAATGACAAAGAAGCAGCATCCCACGCCGCATACCGGCCAATTTCAGCGCCTTTTACAGCAGAAAAGGATTCGCTCGATTACTGGCTGa                                                                <  1:79634/138‑1 (MQ=255)                                                                  aaGTCCGAAAAAAACGGTGATGCCATTGACTATGTCAGCAAGCGGAAAAATGACAAAGAAGCAGCATCCCACGCCTCATACCGGCCAATTTCAGCGCCTTTTACAGCAGAAAAGGATTCGCTCGATTACTGGCTGACa                                                              <  4:172637/138‑1 (MQ=255)                                                                    gTCCGAAAAAAACGGTGATGCCATTGACTATGTCAGCAAGCGGAAAAATGACAAAGAAGCAGCATCCCACGCCGCATACCGGCCAATTTCAGCGCCTTTTACAGCAGAAAAGGATTCGCTCGATTACTGGCTGACAGaa                                                           <  1:67334/139‑1 (MQ=255)                                                                       cGAAAAAAACGGTGATGCCATTGACTATGTCAGCAAGCGGAAAAATGACAAAGACGCTGCATCCCACGCCGCATACCGGCCAATTTCAGCGCCTTATACAGCAGAAAAGGATTCGCTCGATTACTGGCTGACAGAACGa                                                        >  7:294280/1‑139 (MQ=255)                                                                       cGAAAAAAACGGTGATGCCATTGACTATGTCAGCAAGCGGAAAAATGACAAAGAAGCAGCATCCCACGCCGCATACCGGCCAATTTCAGCGCCTTTTACAGCAGAAAAGGATTCGCTCGATTACTGGCTGACAGAACGa                                                        >  3:237479/1‑139 (MQ=255)                                                                       cGAAAAAAACGGTGATGCCATTGACTATGTCAGCAAGCGGAAAAATGACAAAGAAGCAGCATCCCACGCCGCATACCGGCCAATTTCAGCGCCTTTTACAGCAGAAAAGGATTCGCTCGATTACTGGCTGACAGAACGa                                                        >  8:50578/1‑139 (MQ=255)                                                                       cGAAAAAAACGGTGATGCCATTGACTATGTCAGCAAGCGGAAAAATGACAAAGAAGCAGCATCCCACGCCGCATACCGGCCAATTTCAGCGCCTTTTACAGCAGAAAAGGATTCGCTCGATTACTGGCTGACAGAACGa                                                        >  8:55618/1‑139 (MQ=255)                                                                       cGAAAAAAACGGTGATGCCATTGACTATGTCAGCAAGCGGAAAAATGACAAAGAAGCAGCATCCCACGCCGCATACCGGCCAATTTCAGCGCCTTTTACAGCAGAAAAGGATTCGCTCGATTACTGGCTGACAGAACGa                                                        >  7:246186/1‑139 (MQ=255)                                                                       cGAAAAAAACGGTGATGCCATTGACTATGTCAGCAAGCGGAAAAATGACAAAGAAGCAGCATCCCACGCCGCATACCGGCCAATTTCAGCGCCTTTTACAGCAGAAAAGGATTCGCTCGATTACTGGCTGACAGAACGa                                                        >  7:308117/1‑139 (MQ=255)                                                                         aaaaaaaCGGTGATGCCATTGACTATGTCAGCAAGCGGAAAAATGACAAAGAAGCAGCATCCCACGCCGCATACCGGCCAATTTCAGCGCCTTTTACAGCAGAAAAGGATTCGCTCGATTACTGGCTGACAGAACGATa                                                      >  8:63947/1‑139 (MQ=255)                                                                          aaaaaaCGGTGATGCCATTGACTCTGGCAGCAACCCGAAAAATGACAAAGAAGCAGCACCCCACGCCGCATACCGGCCAATTTCAGCGCCTTTTACAGCAGAAAAGGATTCGCTCGATTACTGGCTGACAGAACGATAc                                                     <  4:231875/139‑1 (MQ=255)                                                                          aaaaaaCGGTGATGCCATTGACTATGTCAGCAAGCGGAAAAATGACAAAGAAGCAGCATCCCACGCCGCATACCGGCCAATTTCAGCGCCTTTTACAGCAGAAAAGGATTCGCTCGATTACTGGCTGACAGAACGATAc                                                     <  7:356513/139‑1 (MQ=255)                                                                          aaaaaaCGGTGATGCCATTGACTATGTCAGCAAGCGGAAAAATGACAAAGAAGCAGCATCCCACGCCGCATACCGGCCAATTTCAGCGCCTTTTACAGCAGAAAAGGATTCGCTCGATTACTGGCTGACAGAACGATAc                                                     <  5:356175/139‑1 (MQ=255)                                                                          aaaaaaCGGTGATGCCATTGACTATGTCAGCAAGCGGAAAAATGACAAAGAAGCAGCATCCCACGCCGCATACCGGCCAATTTCAGCGCCTTTTACAGCAGAAAAGGATTCGCTCGATTACTGGCTGACAGAACGATAc                                                     <  4:237479/139‑1 (MQ=255)                                                                          aaaaaaCGGTGATGCCATTGACTATGTCAGCAAGCGGAAAAATGACAAAGAAGCAGCATCCCACGCCGCATACCGGCCAATTTCAGCGCCTTTTACAGCAGAAAAGGATTCGCTCGATTACTGGCTGACAGAACGATAc                                                     <  3:338169/139‑1 (MQ=255)                                                                          aaaaaaCGGTGATGCCATTGACTATGTCAGCAAGCGGAAAAATGACAAAGAAGCAGCATCCCACGCCGCATACCGGCCAATTTCAGCGCCTTTTACAGCAGAAAAGGATTCGCTCGATTACTGGCTGACAGAACGATAc                                                     <  3:292546/139‑1 (MQ=255)                                                                             aaaCGGTGATGCCATTGACTATGTCAGCAAGCGGAAAAATGACAAAGAAGCAGCATCCCACGCCGCATACCGGCCAATTTCAGCGCCTTTTACAGCAGAAAAGGATTCGCTCGATTACTGGCTGACAGAACGATACAgg                                                  <  1:154957/139‑1 (MQ=255)                                                                             aaaCGGTGATGCCATTGACTATGTCAGCAAGCGGAAAAATGACAAAGAAGCAGCATCCCACGCCGCATACCGGCCAATTTCAGCGCCTTTTACAGCAGAAAAGGATTCGCTCGATTACTGGCTGACAGAACGATACAgg                                                  <  2:319315/139‑1 (MQ=255)                                                                                    gATGCCATTGACTATGTCAGCAAGCGGAAAAATGACAAAGAAGCAGCATCCCACGCCGCATACCGGCCAATTTCAGCGCCTTTTACAGCAGAAAAGGATTCGCTCGATTACTGGCTGACAGAACGATACAGGCTCTaca                                           >  1:211621/1‑139 (MQ=255)                                                                                    gATGCCATTGACTATGTCAGCAAGCGGAAAAATGACAAAGAAGCAGCATCCCACGCCGCATACCGGCCAATTTCAGCGCCTTTTACAGCAGAAAAGGATTCGCTCGATTACTGGCTGACAGAACGATACAGGCTCTaca                                           >  6:89685/1‑139 (MQ=255)                                                                                    gATGCCATTGACTATGTCAGCAAGCGGAAAAATGACAAAGAAGCAGCATCCCACGCCGCATACCGGCCAATTTCAGCGCCTTTTACAGCAGAAAAGGATTCGCTCGATTACTGGCTGACAGAACGATACAGGCTCTaca                                           >  1:35522/1‑139 (MQ=255)                                                                                             gACTATGTCAGCAAGCGGAAAAATGACAAAGAAGCAGCATCCCACGCCGCATACCGGCCAATTTCAGCGCCTTTTACAGCAGAAAAGGATTCGCTCGATTACTGGCTGACAGAACGATACAGGCTCTACACCACTTAt                                   <  6:83359/138‑1 (MQ=255)                                                                                             gACTATGTCAGCAAGCGGAAAAATGACAAAGAAGCAGCATCCCACGCCGCATACCGGCCAATTTCAGCGCCTTTTACAGCAGAAAAGGATTCGCTCGATTACTGGCTGACAGAACGATACAGGCTCTACACCACTTAt                                   <  7:55618/138‑1 (MQ=255)                                                                                                 aTGTCAGCAAGCGGAAAAATGACAAAGAAGCAGCATCCCACGCCGCATACCGGCCAATTTCAGCGCCTTTTACAGCAGAAAAGGATTCGCTCGATTACTGGCTGACAGAACGATACAGGCTCTACACCACTTATCGCaa                              >  3:362588/1‑139 (MQ=255)                                                                                                  tGTCAGCAAGCGGAAAAATGACAAAGAAGCAGCATCCCACGCCGCATACCGGCCAATTTCAGCGCCTTTTACAGCAGAAAAGGATTCGCTCGATTACTGGCTGa                                                                <  6:222184/104‑1 (MQ=255)                                                                                                  tGTCAGCAAGCGGAAAAATGACAAAGAAGCAGCATCCCACGCCGCATACCGGCCAATTTCAGCGCCTTTTACAGCAGAAAAGGATTCGCTCGATTACTGGCTGa                                                                >  5:222184/1‑104 (MQ=255)                                                                                                  tGTCAGCAAGCGGAAAAATGACAAAGAAGCAGCATCCCACGCCGCATACCGGCCAATTTCAGCGCCTTTTACAGCAGAAAAGGATTCGCTCGATTACTGGCTGACAGAACGATACAGGCTCTACACCACTTATCGCaa                              >  7:162084/1‑138 (MQ=255)                                                                                                  tGTCAGCAAGCGGAAAAATGACAAAGAAGCAGCATCCCACGCCGCATACCGGCCAATTTCAGCGCCTTTTACAGCAGAAAAGGATTCGCTCGATTACTGGCTGACAGAACGATACAGGCTCTACACCACTTATCGCaa                              >  4:247057/1‑138 (MQ=255)                                                                                                   gTCAGCAAGCGGAAAAATGACAAAGAAGCAGCATCCCACGCCGCATACCGGCCAATTTCAGCGCCTTTTACAGCAGAAAAGGATTCGCTCGATTACTGGCTGACAGAACGATACAGGCTCTACACCACTTATCGCAAt                             >  3:376233/1‑138 (MQ=255)                                                                                                      aGCAAGCGGAAAAATGACAAAGAAGCAGCATCCCACGCCGCATACCGGCCAATTTCAGCGCCTTTTACAGCAGAAAAGGATTCGCTCGATTACTGGCTGACAGAACGATACAGGCTCTACACCACTTATCGCAATAAg                          <  4:139841/138‑1 (MQ=255)                                                                                                      aGCAAGCGGAAAAATGACAAAGAAGCAGCAGCCCACGCCGCATACCGGCCAATTTCAGCGCCTTTTACAGCAGAAAAGGATTCGCTCGATTACTGGCTGACAGAACGATACAGGCTCTACACCACTTATCGCAATAAg                          <  7:221661/138‑1 (MQ=255)                                                                                                       gCAAGCGGAAAAATGACAAAGAAGCAGCATCCCACGGCGCATACCGGCCAATTTCAGCGCCTTTTACAGGAGAAAAGGATTCGCTCGATTACTGGCTGACAGAACGATACTGGCTCTACACCACTTATCGCAATAAGct                        <  6:336994/139‑1 (MQ=255)                                                                                                                      cccaagaagCAGCATCCCACGCCGCATACCGGCCAATTTCAGCGCCTTTTACAGCAGAAAAGGATTCGCTCGATTACTGGCAGACAGAACGATACAGGCTCTACACCACTTATCGCAATAAGCTCTATTACGAAGAcat         <  8:294280/136‑1 (MQ=255)                                                                                                                      aCAAAGAAGCAGCATCCCACGCCGCATACCGGCCAATTTCAGCGCCTTTTACAGCAGAAAAGGATTCGCTCGATTACTTGCTGACAGAACGATACAgg                                                  >  3:312801/1‑98 (MQ=255)                                                                                                                      aCAAAGAAGCAGCATCCCACGCCGCATACCGGCCAATTTCAGCGCCTTTTACAGCAGAAAAGGATTCGCTCGATTACTAGCTGACAGAACGATACAgg                                                  <  4:312801/98‑1 (MQ=255)                                                                                                                       cAAAGAAGCAGCATCCCACGCCGCATACCGGCCAATTTCAGCGCCTTTTACAGCAGAAAAGGATTCGCTCGATTACTGGCTGACAGAACGATACAGGCTCTACACCACTTATCGCAATAAGCTCTATTACGAAGAcatc        <  5:63478/139‑1 (MQ=255)                                                                                                                            aagCAGCATCCCACGCCGCATACCGGCCAATTTCAGCGCCTTTTACAGCAGAAAAGGATTCGCTCGATTACTGGCTGACAGAACGATACAGGCTCTACACCACTTATCGCAATAAGct                        <  4:206440/118‑1 (MQ=255)                                                                                                                            aagCAGCATCCCACGCCGCATACCGGCCAATTTCAGCGCCTTTTACAGCAGAAAAGGATTCGCTCGATTACTGGCTGACAGAACGATACAGGCTCTACACCACTTATCGCAATAAGct                        >  3:206440/1‑118 (MQ=255)                                                                                                                            aagCAGCATCCCACGCCGCATACCGGCCAATTTCAGCGCCTTTTACAGCAGAAAAGGATTCGCTCGATTACTGGCTGACAGAACGATACAGGCTCTACACCACTTATCGCAATAAGCTCTATTACGAAGACATCcatca   <  8:308117/139‑1 (MQ=255)                                                                                                                             agcagcATCCCACGCCGCATACCGGCCAATTTCAGCGCCTTTTACAGCAGAATAGGATTCGCTCGATTAATGGCTGACAGAACGATAGAGGCTCTTCACCACTTATCGCAATAAGCTCTATTAGGAAGAGATCcatcat  <  8:66597/139‑1 (MQ=255)                                                                                                                                     |                                                                                                                                   ATCATCGTCTGGCGGTCCTTGGCGCTCGCACATTCTTTCATCTGCCTTATTTTTACGCGGATATGAAGTCCGAAAAAAACGGTGATGCCATTGACTATGTCAGCAAGCGGAAAAATGACAAAGAAGCAGCATTCCACGCCGCATACCGGCCAATTTCAGCGCCTTTTACAGCAGAAAAGGATTCGCTCGATTACTGGCTGACAGAACGATACAGGCTCTACACCACTTATCGCAATAAGCTCTATTACGAAGACATCCATCAT  >  BS168_reference_genome/2485227‑2485489

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑