BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	BS168_reference_genome	4,155,390:1	+A	100%	intergenic (‑181/+43)	trnY‑Lys ← / ← purA	tRNA‑Lys/adenylosuccinate synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	BS168_reference_genome	4,155,390	1	.	A	97.9%	327.8 / ‑1.1	93	intergenic (‑181/+43)	trnY‑Lys/purA	tRNA‑Lys/adenylosuccinate synthetase
Reads supporting (aligned to +/- strand): ref base . (0/2); new base A (37/54); total (37/56)
Fisher's exact test for biased strand distribution p-value = 5.16e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.77e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.
Rejected as polymorphism: Polymorphic indel expands or contracts a homopolymer stretch.

Only 100 of 101 total aligned reads displayed.

GTTCTCATCTTGTACTCTATTGAAGCAGTTTCCGTAACAACGAGATTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTT‑CAAAC‑AAAAA‑CAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGCACGGTACACACTGCGAAGGACATTTGTTTGTGAGCGGTCTGGACCGACAGAGAAAATAGAAAGCGGAATGCCTGTCAG  >  BS168_reference_genome/4155253‑4155519

ggtctcATCTTGTACGCTATTGAAGCAGTTTCCGTAACAACGAGATTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTT‑caaac‑a                                                                                                                                   <  4:111701/137‑1 (MQ=255)

      aTCTTGTACTCTATTGAAGCAGTTTCCGTAACAACGAGATTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTT‑Caaac‑aaaaaa                                                                                                                              >  5:272317/1‑137 (MQ=255)

           gTACTCTATTGAAGCAGTTTCCGTAACAACGAGATTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTT‑Caaac‑aaaaaacaa                                                                                                                           <  1:259902/136‑5 (MQ=255)

           gTACTCTATTGAAGCAGTTTCCGTAACAACGAGATTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTT‑Caaac‑aaaaaacaa                                                                                                                           <  8:112661/136‑5 (MQ=255)

           gTACTCTATTGAAGCAGTTTCCGTAACAACGAGATTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTT‑Caaac‑aaaaaacaa                                                                                                                           <  7:262748/136‑5 (MQ=255)

           gTACTCTATTGAAGCAGTTTCCGTAACAACGAGATTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTT‑Caaac‑aaaaaacaa                                                                                                                           <  2:46445/136‑5 (MQ=255)

           gTACTCTATTGAAGCAGTTTCCGTAACAACGAGATTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGGTTTTTTTTAATATTTTTTTATAAGACCAACTTATATGCGGCTTT‑AAAACAAAAAA‑CAAGcc                                                                                                                        >  4:120590/1‑139 (MQ=255)

              ctctATTGAAGCAGTTTCCGTAACAACGAGATTTATAATATCACGCACAAATAGAAGTGGCAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTT‑CAAACAAAAAA‑CAAGcccc                                                                                                                      <  1:14843/138‑1 (MQ=255)

              ctctATTGAAGCAGTTTCCGTAACAACGAGATTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTT‑CAAACAAAAAA‑CAAGcccc                                                                                                                      <  5:120281/138‑1 (MQ=255)

              ctctATTGAAGCAGTTTCCGTAACAACGAGATTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTT‑CAAACAAAAAA‑CAAGcccc                                                                                                                      <  3:36066/138‑1 (MQ=255)

                 tATTGAAGCAGTTTCCGTAACAACGAGATTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTT‑CATACAAAAAA‑CAAGCCCCTTa                                                                                                                   >  8:283180/1‑138 (MQ=255)

                 tATTGAAGCAGTTTCCGTAACAACGAGATTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTT‑CAAACAAAAAA‑CAAGcccc                                                                                                                      <  2:67322/135‑1 (MQ=255)

                 tATTGAAGCAGTTTCCGTAACAACGAGATTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTT‑CAAACAAAAAA‑CAAGcccc                                                                                                                      <  3:220196/135‑1 (MQ=255)

                 tATTGAAGCAGTTTCCGTAACAACGAGATTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTT‑CAAACAAAAAA‑CAAGcccc                                                                                                                      >  1:67322/1‑135 (MQ=255)

                 tATTGAAGCAGTTTCCGTAACAACGAGATTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTT‑CAAACAAAAAA‑CAAGcccc                                                                                                                      >  4:220196/1‑135 (MQ=255)

                 tATTGAAGCAGTTTCCGTAACAACGAGATTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTT‑CAAACAAAAAA‑CAAGc                                                                                                                         <  8:222416/132‑1 (MQ=255)

                 tATTGAAGCAGTTTCCGTAACAACGAGATTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTT‑CAAACAAAAAA‑CAAGc                                                                                                                         >  7:222416/1‑132 (MQ=255)

                 tATTGAAGCAGTTTCCGTAACAACGAGATTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTT‑CAAACAAAAAA‑CAAGCCCCTTa                                                                                                                   >  3:141935/1‑138 (MQ=255)

                 tATTGAAGCAGTTTCCGTAACAACGAGATTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTT‑CAAACAAAAAA‑CAAGCCCCTTa                                                                                                                   >  3:173523/1‑138 (MQ=255)

                 tATTGAAGCAGTTTCCGTAACAACGAGATTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTT‑CAAACAAAAAA‑CAAGCCCCTTa                                                                                                                   >  3:208836/1‑138 (MQ=255)

                 tATTGAAGCAGTTTCCGTAACAACGAGATTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTT‑CAAACAAAAAA‑CAAGCCCCTTa                                                                                                                   >  6:35836/1‑138 (MQ=255)

                 tATTGAAGCAGTTTCCGTAACAACGAGATTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTT‑CAAACAAAAAA‑CAAGCCCCTTa                                                                                                                   >  5:145994/1‑138 (MQ=255)

                   ttGAAGCAGTTTCCGTAACAACGAGATTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTT‑CAAACAAAAAA‑CAAGCCCCTTaaa                                                                                                                 >  1:98061/1‑138 (MQ=255)

                         cAGTTTCCGTAACAACGAGATTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTT‑CAAACAAAAAA‑CAAGCCCCTTAAATAGGGGc                                                                                                          >  5:306026/1‑139 (MQ=255)

                           gTTTCCGTAACAACGAGATTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTT‑Caaac‑aaaaaacaag                                                                                                                          >  2:251161/1‑116 (MQ=255)

                           gTTTCCGTAACAACGAGATTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTT‑Caaac‑aaaaaacaag                                                                                                                          <  1:251161/121‑6 (MQ=255)

                           gTTTCCGTAACAACGAGATTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTT‑CAAACAAAAAA‑CAAGCCCCTTAAATAGGGGCtt                                                                                                        >  7:310604/1‑139 (MQ=255)

                           gTTTCCGTAACAACGAGATTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTT‑CAAACAAAAAA‑CAAGCCCCTTAAATAGGGGCtt                                                                                                        >  1:249543/1‑139 (MQ=255)

                            tttCCGTAACAACGAGATTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTT‑CAAACAAAAAA‑CAAGCCCCTTAAATAGGGGCTTg                                                                                                       <  1:116760/139‑1 (MQ=255)

                            tttCCGTAACAACGAGATTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTT‑CAAACAAAAAA‑CAAGCCCCTTAAATAGGGGCTTg                                                                                                       <  2:249543/139‑1 (MQ=255)

                            tttCCGTAACAACGAGATTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTT‑CAAACAAAAAA‑CAAGCCCCTTAAATAGGGGCTTg                                                                                                       <  8:63217/139‑1 (MQ=255)

                            tttCCGTAACAACGAGATTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTT‑CAAACAAAAAA‑CAAGCCCCTTAAATAGGGGCTTg                                                                                                       >  1:2914/1‑139 (MQ=255)

                                  tAACAACGAGATTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTT‑CAAACAAAAAA‑CAAGCCCCTTAAATAGGGGCTTGCAGACa                                                                                                 >  4:64751/1‑139 (MQ=255)

                                    acaacGAGATTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTT‑CAAACAAAAAA‑CAAGCCCCTTAAATAGGGGCTTGCAGACata                                                                                               <  5:93229/139‑1 (MQ=255)

                                    acaacGAGATTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTT‑CAAACAAAAAA‑CAAGCCCCTTAAATAGGGGCTTGCAGACata                                                                                               <  6:306026/139‑1 (MQ=255)

                                      aacGAGATTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTT‑CAAACAAAAAA‑CAAGCCCCTTAAATAGGGGCTTGCAGAc                                                                                                  >  4:63906/1‑134 (MQ=255)

                                      aacGAGATTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTT‑CAAACAAAAAA‑CAAGCCCCTTAAATAGGGGCTTGCAGAc                                                                                                  <  3:63906/134‑1 (MQ=255)

                                      aacGAGATTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACAAACTTATATGCGGCTTT‑CAAACAAAAAA‑CAAGCCCCTTAAATAGGGGCTTGCAGACATAtt                                                                                             <  3:146118/139‑1 (MQ=255)

                                      aacGAGATTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGGTTTTTTTTCATATTTTTATCTCCGACCAACTTATATGCGGCTTT‑CAAACAAAAAA‑CAAGCCCCTTAAATAGGGGCTTGCAGACATAtt                                                                                             <  8:38287/139‑1 (MQ=255)

                                          agaTTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTT‑CAAACAAAAAA‑CAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTAt                                                                                         <  3:64751/139‑1 (MQ=255)

                                                ttaataTCACGAACAAATTGAAGTTGAAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCTGACCAACTTATATGCGGCTTT‑CAAACAAAAAA‑CAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGt                                                                                    <  2:252283/137‑1 (MQ=255)

                                                ataataTCACGAACAAATAGAAGTGGCAATAAATTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTT‑CAAACAAAAAA‑CAAGCCCCTTATATAGGGTCTTGCAGACATATTCTATTTAGt                                                                                    >  3:148373/1‑138 (MQ=255)

                                                 taataTCACGAACAAATAGAAGTGGCAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTT‑CAAACAAAAAA‑CAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTc                                                                                  <  6:160679/139‑1 (MQ=255)

                                                   atatCACGAACAAATAGAAGTGGCAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTT‑CAAACAAAAAA‑CAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGc                                                                                <  4:141935/139‑1 (MQ=255)

                                                   atatCACGAACAAATAGAAGTGGCAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTT‑CAAACAAAAAA‑CAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGc                                                                                <  5:73662/139‑1 (MQ=255)

                                                   atatCACGAACAAATAGAAGTGGCAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTT‑CAAACAAAAAA‑CAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGc                                                                                <  4:208836/139‑1 (MQ=255)

                                                   atatCACGAACAAATAGAAGTGGCAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTT‑CAAACAAAAAA‑CAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGc                                                                                <  5:156031/139‑1 (MQ=255)

                                                   atatCACGAACAAATAGAAGTGGCAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTT‑CAAACAAAAAA‑CAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGc                                                                                <  6:40236/139‑1 (MQ=255)

                                                   atatCACGAACAAATAGAAGTGGCAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTT‑CAAACAAAAAA‑CAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGc                                                                                <  6:45422/139‑1 (MQ=255)

                                                   atatCACGAACAAATAGAAGTGGCAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTT‑CAAACAAAAAA‑CAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGc                                                                                <  3:153780/139‑1 (MQ=255)

                                                   atatCACGAACAAATAGAAGTGGCAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTT‑CAAACAAAAAA‑CAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGc                                                                                <  2:98061/139‑1 (MQ=255)

                                                   atatCACGAACAAATAGAAGTGGCAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTT‑CAAACAAAAAA‑CAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGc                                                                                <  2:2914/139‑1 (MQ=255)

                                                   atatCACGAACAAATAGAACTGGCAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTT‑CAAAC‑AAAAACCAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGc                                                                                <  7:157618/139‑1 (MQ=255)

                                                   atatCACGAACAAAAAGAAGTGGCAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTT‑CAAACAAAAAA‑CAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGc                                                                                <  1:289550/139‑1 (MQ=255)

                                                   atatCACCAACAAATAGACGTGGCAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTT‑CAAACAAAAAA‑CAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGc                                                                                <  5:172227/139‑1 (MQ=255)

                                                   atatCACCAACAAATAGAAGTGGCAATAACTTTTTTTGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTT‑CAAACAAAAAA‑CAAGCCCCTTAAAAAGGGGCTTGCAGACATATTCTATTTAGTTCGc                                                                                <  1:103416/139‑1 (MQ=255)

                                                   atatAACGAAAAAATAGAAGTGCCAATAACTTTTTTGTTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTT‑CAAACAAAAAA‑CAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGc                                                                                <  3:120590/139‑1 (MQ=255)

                                                     atCACGAACAAATAGAAGTGGCAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTT‑CAAACAAAAAA‑CAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGCAc                                                                              <  1:92405/139‑1 (MQ=255)

                                                      tCACGAACAAATAGAAGTGGCAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTT‑CAAACAAAAAA‑CAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGCACg                                                                             >  7:213804/1‑139 (MQ=255)

                                                      tCACGAACAAATAGAAGTGGCAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTT‑CAAACAAAAAA‑CAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGCACg                                                                             >  3:115162/1‑139 (MQ=255)

                                                      tCACGAACAAATAGAAGCGGCAATAACTTTTTTGGGTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTT‑CAAACAAAAAA‑CAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGCAc                                                                              <  1:86417/138‑1 (MQ=255)

                                                         cGAACAAATAGAAGTGGCAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTT‑CAAACAAAAAA‑CAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGc                                                                                >  7:79264/1‑133 (MQ=255)

                                                         cGAACAAATAGAAGTGGCAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTT‑CAAACAAAAAA‑CAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGc                                                                                <  8:79264/133‑1 (MQ=255)

                                                           aaCAAATAGAAGTGGCAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTT‑CAAACAAAAAA‑CAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGCACGGTaca                                                                        <  5:35836/139‑1 (MQ=255)

                                                           aaCAAATAGAAGTGGCAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTT‑CAAACAAAAAA‑CAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGCACGGTaca                                                                        >  6:199254/1‑139 (MQ=255)

                                                           aaCAAATAGAAGTGGCAATAACTTTTTTGGTATTTTTTCATATTTATTTCTCAGACCAACTTATATGCGGCTTT‑CAAACAAAAAA‑CAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGCACGGTaca                                                                        <  1:6281/139‑1 (MQ=255)

                                                             cAAATAGAAGTGGCAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTT‑CAAACAAAAAA‑CAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGCACGGTacaca                                                                      <  3:118564/139‑1 (MQ=255)

                                                                aTAGAAGTGGCAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTT‑CAAACAAAAAA‑CAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGCACGGTACACACTg                                                                   >  4:170603/1‑139 (MQ=255)

                                                                   gAAGTGGCAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTT‑CAAACAAAAAA‑CAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGCACGGTACACACTGCg                                                                 <  4:173523/138‑1 (MQ=255)

                                                                   gAAGTGGCAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTT‑CAAACAAAAAA‑CAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGCACGGTACACACTGCg                                                                 <  1:272313/138‑1 (MQ=255)

                                                                   gAAGTGGCAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTT‑CAAACAAAAAA‑CAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGCACGGTACACACTGCg                                                                 <  3:308870/138‑1 (MQ=255)

                                                                      gTGGCAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCACCTTATATGCGGCTTT‑CAAACAAAAAA‑CAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGCACGGTACACACTGCGAAg                                                              >  3:194595/1‑138 (MQ=255)

                                                                        ggCAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTT‑CAAACAAAAAA‑CAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGCACGGTACACACTGCGAAGGa                                                            >  6:110192/1‑138 (MQ=255)

                                                                        ggCAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTT‑CAAACAAAAAA‑CAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGCACGGTACACACTGCGAAGGa                                                            >  3:2589/1‑138 (MQ=255)

                                                                         gCAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTT‑CAAACAAAAAA‑CAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGCACGGt                                                                           >  7:223026/1‑122 (MQ=255)

                                                                         gCAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTT‑CAAACAAAAAA‑CAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGCACGGTACACACTGCGAAGGACa                                                          >  5:276423/1‑139 (MQ=255)

                                                                         gCAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACAAACTTATATGCGGCTTT‑CAAACAAAAAA‑CAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGCACGGt                                                                           <  8:223026/122‑1 (MQ=255)

                                                                          cAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTT‑CAAACAAAAAA‑CAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGCACGGTaca                                                                        >  8:40820/1‑124 (MQ=255)

                                                                          cAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTT‑CAAACAAAAAA‑CAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGCACGGTaca                                                                        <  7:40820/124‑1 (MQ=255)

                                                                          cAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTT‑CAAACAAAAAA‑CAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGCACGGTACACACTGCGAAGGACAt                                                         >  6:119841/1‑139 (MQ=255)

                                                                              aaCTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTT‑CAAACAAAAAA‑CAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGCACGGTACACACTGCGAAGGACAtttgt                                                     >  5:357/1‑139 (MQ=255)

                                                                                    tttGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTT‑CAAACAAAAAA‑CAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGc                                                                                <  8:63379/106‑1 (MQ=255)

                                                                                    tttGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTT‑CAAACAAAAAA‑CAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGc                                                                                >  7:63379/1‑106 (MQ=255)

                                                                                     ttGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTT‑CAAACAAAAAA‑CAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGCACGGTACACACTGCGAAGGACATTTGTTTGTGa                                               <  8:213804/138‑1 (MQ=255)

                                                                                      tGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTT‑CAAACAAAAAA‑CACGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGCACGGTACACACTGCGAAGGACATTTGTTTGTGAg                                              <  7:59444/138‑1 (MQ=255)

                                                                                      tGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTT‑CAAACAAAAAA‑CAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGCACGGTACACACTGCGAAGGACATTTGTTTGTGAg                                              <  6:272317/138‑1 (MQ=255)

                                                                                      tGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTT‑CAAACAAAAAA‑CAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGCACGGTACACACTGCGAAGGACATTTGTTTGTGAg                                              <  5:119841/138‑1 (MQ=255)

                                                                                      tGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTT‑CAAACAAAAAA‑CAAGCCCCTTAAATAGGGGATTGCAGACATATTCTATTTAGTTCGCACGGTACACACTGCGAAGGACATTTGTTTGTGAg                                              <  3:260900/138‑1 (MQ=255)

                                                                                        gTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTT‑CAAACAAAAAA‑CAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGCACGGTACACACTGCGAAGGACATTTGTTTGTGAGCgg                                           >  2:221910/1‑139 (MQ=255)

                                                                                                   tatTTTTTTCTCAGACCAACTTATATGCGGCTTT‑CAAACAAAAAA‑CAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGCACGGTACACACTGCGAAGGACATTTGTTTGTGAGCGGTCTGGACCGAc                                <  5:110192/139‑1 (MQ=255)

                                                                                                    ttttttttCTAAGACCAATTTATATGCGGATTTAAAAAA‑AAAAA‑CAAGCCCATTAATTCGGGGCTTTCAGACATATTCTATTTAGTTCGCACTGTACACACTGCGAAGGACATTTGTTTGTGAGCGGTCTGGACCGAc                                <  2:159832/137‑1 (MQ=255)

                                                                                                               aGACCAACTTATATGCGGCTTT‑CAAACAAAAAA‑CAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGCACGGTACACACTGCGAAGGACATTTGTTTGTGAGCGGTCTGGACCGACAGAGAAAATAGa                    <  1:73452/139‑1 (MQ=255)

                                                                                                               aGACCAACTTATATGCGGCTTT‑CAAACAAAAAA‑CAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGCACGGTACACACTGCGAAGGACATTTGTTTGTGAGCGGTCTGGACCGACAGAGAAAATAGa                    <  4:276212/139‑1 (MQ=255)

                                                                                                                gACCAACTTATATGCGGCTTT‑CAAACAAAAAA‑CAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGCACGGTACACACTGCGAAGGACATTTGTTTGTGAGCGGTCTGGACCGACAGAGAAAATAGa                    >  1:18847/1‑138 (MQ=255)

                                                                                                                          tatGCGGATTT‑CAAACAAAAAA‑CAAGCAGCTTAAATAGGGGCTTGCAGACATTTTCTATTTAGTTCGCACGGTACACACTGCGAAGGACTTTTGTTTGTGAGCGGTCTGGACCGACAGAGAAAATAGAAAGCGGAATg          <  4:148373/138‑1 (MQ=255)

                                                                                                                           atGCGGCTTT‑CAAACAAAAAA‑CAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGCACGGTACACACTGCGAAGGACATTTGTTTGTGAGCGGTCTGGACCGACAGAGAAAATAGAAAGCGGAATGcc        >  1:228169/1‑139 (MQ=255)

                                                                                                                              cGGCTTT‑CAAACAAAAAA‑CAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGCACGGTACACACTGCGAAGGACATTTGTTTGTGAGCGGTCTGGACCGACAGAGAAAATAGAAAGCGGAATGCCTGt     <  2:228169/139‑1 (MQ=255)

                                                                                                                              cGGCTTT‑CAAACAAAAAA‑CAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGCACGGTACACACTGCGAAGGACATTTGTTTGTGAGCGGTCTGGACCGACAGAGAAAATAGAAAGCGGAATGCCTGt     <  4:115162/139‑1 (MQ=255)

                                                                                                                                 cTTT‑CAAACAAAAAA‑CAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGCACGGTACACACTGCGAAGGACATTTGTTTGTGAGCGGTCTGGACCGACAGAGAAAATAGAAAGCGGAATGCCTGTCAg  >  2:213677/1‑139 (MQ=255)

                                                                                                                                 cTTT‑CAAACAAAAAA‑CAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGCACGGTACACACTGCGAAGGACATTTGTTTGTGAGCGGTCTGGACCGACAGAGAAAATAGAAAGCGGAATGCCTGTCAg  >  1:184887/1‑139 (MQ=255)

GTTCTCATCTTGTACTCTATTGAAGCAGTTTCCGTAACAACGAGATTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTT‑CAAAC‑AAAAA‑CAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGCACGGTACACACTGCGAAGGACATTTGTTTGTGAGCGGTCTGGACCGACAGAGAAAATAGAAAGCGGAATGCCTGTCAG  >  BS168_reference_genome/4155253‑4155519

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`