Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	BS168_reference_genome	165,825:1	+C	100%	noncoding (72/72 nt)	trnI‑Asn →	tRNA‑Asn

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	BS168_reference_genome	165,825	1	.	C	100.0%	161.1 / NA	48	noncoding (72/72 nt)	trnI‑Asn	tRNA‑Asn
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (27/21);  total (27/21)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

GTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATATCTCATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAG‑CATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTAAATGTATTATTACCAAGGTTTCTCATAAGGAGAAAGCTTTTTTTATTGCG  >  BS168_reference_genome/165690‑165954                                                                                                                                         |                                                                                                                                  gTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAG‑ccatt                                                                                                                              <  5:75419/139‑5 (MQ=21)    ggACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGc                                                                                                                             <  5:287267/138‑1 (MQ=21)        gccgccAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCa                                                                                                                        <  2:93025/139‑1 (MQ=21)                    aTTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAgg                                                                                                            <  6:279030/139‑1 (MQ=21)                     tttcataAATCTACCATGCTTTCTTCTGAAGCGTAATAT‑T‑ATTCCGCAGTAGCGCAGTGGAAGAGCTATCGGCTGTTCACCGATCGGTCGTCGGCTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCCGCAgg                                                                                                            <  4:76028/133‑1 (MQ=9)                        attaATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑AATCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTCGGGGCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTag                                                                                                         <  7:295777/138‑1 (MQ=18)                         ttaATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTagag                                                                                                       <  7:140177/139‑1 (MQ=21)                          taATGCTACCATGTTTGTTGATGCAGCTCTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCGCAGCAGGTagag                                                                                                       <  5:13678/138‑1 (MQ=17)                                  ccATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGAAGTCTCTCCGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCa                                                                                               <  6:45886/138‑1 (MQ=21)                                                                ttCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCTGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGcc                                                                  >  7:212424/1‑139 (MQ=255)                                                                ttCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGcc                                                                  >  6:223660/1‑139 (MQ=255)                                                                ttCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGcc                                                                  >  1:274135/1‑139 (MQ=255)                                                                ttCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGcc                                                                  >  5:154538/1‑139 (MQ=255)                                                                ttCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCGCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGcc                                                                  >  3:174347/1‑139 (MQ=255)                                                                     cAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCa                                                                                                                        <  8:46378/80‑1 (MQ=9)                                                                     cAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCa                                                                                                                        >  7:46378/1‑80 (MQ=9)                                                                     cAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCtt                                                             >  3:219322/1‑139 (MQ=255)                                                                       gTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTgg                                                           <  2:211212/139‑1 (MQ=255)                                                                       gTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGGTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTgg                                                           <  6:186203/139‑1 (MQ=255)                                                                       gTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGGCGTAGGTTCGACTCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTgg                                                           <  3:91967/139‑1 (MQ=255)                                                                        tAGGTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTTGGTTCGAATCCTACCTGCGGAGCCATTGCTTCAATAGCTCAGCAGGTAGAGCACTTCAATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTgg                                                           <  6:123282/138‑1 (MQ=25)                                                                        tAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGa                                                          <  8:212424/139‑1 (MQ=255)                                                                           cTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAGGc                                                       >  5:166967/1‑139 (MQ=255)                                                                                tGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTaaa                                                  >  3:246270/1‑139 (MQ=255)                                                                                  gTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTagag                                                                                                       <  6:91253/84‑1 (MQ=255)                                                                                  gTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTagag                                                                                                       >  5:91253/1‑84 (MQ=255)                                                                                       gCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTAAATGtatt                                            <  7:122877/138‑1 (MQ=255)                                                                                          aTCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTAAATGTAttatta                                        >  7:281627/1‑139 (MQ=255)                                                                                          aTCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTAAATGTAttatta                                        >  5:75570/1‑139 (MQ=255)                                                                                          aTCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTAAATGTAttatta                                        >  2:24681/1‑139 (MQ=255)                                                                                          aTCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTAAATGTAttatta                                        >  4:44228/1‑139 (MQ=255)                                                                                          aTCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTAAATGTAttatta                                        >  4:49297/1‑139 (MQ=255)                                                                                                  ttAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCCCTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTAAATGTATTATTACCAAGGtt                                >  7:154807/1‑139 (MQ=255)                                                                                                  ttAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTAAATGTATTATTACCAAGGtt                                >  5:260329/1‑139 (MQ=255)                                                                                                  ttAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTAAATGTATTATTACCAAGGt                                 >  8:296838/1‑138 (MQ=255)                                                                                                  ttAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTAAATGTATTATTACCAAGGt                                 <  6:260329/138‑1 (MQ=255)                                                                                                  ttAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTAAATGTATTATTACCAAGGt                                 <  7:296838/138‑1 (MQ=255)                                                                                                    aaCCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTACATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTTGAAGCTTAAATGTATTATTACCAAGGttt                               <  5:292816/138‑1 (MQ=255)                                                                                                     aCCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTAAATGTATTATTACCAAGGTTtct                             >  2:48634/1‑139 (MQ=255)                                                                                                       cGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTAAATGTATTATTACCAAGGTTTCTCa                           >  6:146987/1‑139 (MQ=255)                                                                                                       cGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTACATTGTAAGGAAGACGTCAGAGGTTCGAGCCCGCTTGTAAGCTTAAATGTATTATTACCAAGCTTTCTCa                           >  2:110180/1‑139 (MQ=38)                                                                                                        gATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTGCGAGCCCGCTTGGAAGCTTAAATGTATTATTACCAAGGTTTCTCa                           >  8:109722/1‑138 (MQ=255)                                                                                                         aTCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTAAATGTATTATTACCAAGGTTTCTCAt                          >  5:239897/1‑138 (MQ=255)                                                                                                         aTCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTAAATGTATTATTACCAAGGTTTCTCAt                          >  6:29922/1‑138 (MQ=255)                                                                                                                 tAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTAAATGTATTATTACCAAGGTTTCTCATAAGgaga                   >  4:293795/1‑137 (MQ=255)                                                                                                                        gAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGAACTTCCATGGTAAGGAAGAGGTAAGCGGGTCGCGACCGCTTGGAAGCTGAAATTTATTATTACAAAGGTTTCTCATAAGGAGAAAGCgtttt          >  5:269382/1‑139 (MQ=255)                                                                                                                           tCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTAAATGTATTATTACCAAGGTTTCTCATAAGGAGAAAGCTTTTTTTa       <  3:44228/139‑1 (MQ=255)                                                                                                                               aCCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTAAATGTATTATTACCAAGGTTTCTCATAAGGAGAAAGCTTTTTTTATTGc   >  6:66152/1‑139 (MQ=255)                                                                                                                                ccTGCGGAGCCATTGCATCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGGCAGCGGTTCGAGCCCGCTTGGAAGCTTAAATGTATTATTACCAAGGTTTCTCATAAGGAGAAAGCTTTTTTTATTGCg  <  6:150062/139‑1 (MQ=255)                                                                                                                                         |                                                                                                                                  GTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATATCTCATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAG‑CATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTAAATGTATTATTACCAAGGTTTCTCATAAGGAGAAAGCTTTTTTTATTGCG  >  BS168_reference_genome/165690‑165954

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑