New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	BS168_reference_genome	= 2653333	46 (0.960)	3 (0.070)	3/222	NT	9.7%	pseudogene (341/471 nt)	sigKn	RNA polymerase sporulation‑specific sigma‑K factor precursor (Sigma‑27) (N‑terminal half)
	?	BS168_reference_genome	2701367 =	12 (0.260)					pseudogene (30/417 nt)	sigKc	RNA polymerase sporulation‑specific sigma‑K factor precursor (Sigma‑27) (C‑terminal fragment)

AAAGTTCGAAAATACAGGTGAGGATGCAGAGGACTTAATCTCCATCGGAACGATCGGGCTTATTAAAGGAATTGAAAGCTATTCCGCTGGAAAAGGGACAAAGCTGGCGACGTATGCAGCGAGGTGTATTGAAAATGAGATTGTAATTACAAAAGGGGGGTGCATACACCCCTCTTTAATACGTTTCAATATATATGGTGTCAGAATCCACAATGGTAACTTCTTTCACGATAAAGTTAACAATTGTTTTTTTATCTTC  >  BS168_reference_genome/2653196‑2653454                                                                                                                                          |                                                                                                                          aaaGTTCGAAAATACAGGTGAGGATGCAGAGGACTTAATCTCCATCGGAACGATCGGGCTTATTAAAGGAATTGAAAGCTATTCCGCTGGAAAAGGGACAAAGCTGGCGACGTATGCAGCGAGGTGTATTGAAAATgag                                                                                                                          <  1:189111/139‑1 (MQ=255)       cgtaaaTACAGGTGATGATGCAGAGGACTTAATCTACATCGGAACGATCGGGCTTATTAAAGGAATTGTAAGCTATTCCGCTGGAATAGGGACAAAGCTTGCGACGTATGCAGCGAGGTGTATTGAAAATGAGATTGt                                                                                                                     <  7:2565/135‑1 (MQ=255)       cGAAAATACAGGTGAGGATGCAGAGGACTTAATCTCCATCGGAACGATCGGGCTTATTAAAGGAATTGAAAGCTATTCCGCTGGAAAAGGGACAAAGCTGGCGACGTATGCAGCGAGGTGTATTGAAAATGAGATTGt                                                                                                                     >  6:217533/1‑138 (MQ=255)                  gTGAGGATGCAGAGGACTTAATCTCCATCGGAACGATCGGGCTTATTAAAGGAATTGAAAGCTATTCCGCTGGAAAAGGGACAAAGCTGGCGACGTATGCAGCGAGGTGTATTGAAAATGAGATTGTAATTACAAAAgg                                                                                                         >  2:7243/1‑139 (MQ=255)                     aGGATGCCGAGGACTTAATCTCCATCGGAACGATCGGGCTTATTAAAGGAATTGAAAGCTATTCCGCTGGAAAAGGGACAAAGCTGGCGACGTATGCAGCGAGGTGTATTGAAAATGAGATTGTAATTACAAAAggggg                                                                                                      <  5:180033/139‑1 (MQ=255)                            agagGACTTAATCTCCATCGGAACGATCGGGCTTATTAAAGGAATTGAAAGCTATTCCGCTGGAAAAGGGACAAAGCGGGCGACGTATGCAGCGAGGTGTATTGAAAATGAGATTGTAATTACAAAAGGGGGGTGCATa                                                                                               <  8:126488/139‑1 (MQ=255)                               ggACTTAATCTCCATCGGAACGATCGGGCTTATTAAAGGAATTGAAAGCTATTCCGCTGGAAAAGGGACAAAGCTGGCGACGTATGCAGCGAGGTGTATTGAAAATGAGATTGTAATTACAAAAGGGGGGTGCATacac                                                                                            <  1:87164/139‑1 (MQ=255)                                gACTTAATCTCCATCGGAACGATCGGGCTTATTAAAGGAATTGAAAGCTATTCCGCTGGAAAAGGGACAAAGCTGGCGACGTATGCAGCGAGGTGTATTGAAAATGAGATTGTAATTACAAAAGGGGGGTGCATACAcc                                                                                           >  3:215471/1‑139 (MQ=255)                                 acatAATCCCCATCGGAACGATCGGGCTTATTAAAGGAATTGAAAGCTATTCCGCTGGAAAAGGGACACAGCTGGCGACGTATGCAGCGAGGTGAATTGAAAATGAGATTGTAATTACAAAAGGGGGGTGCATACAccc                                                                                          <  5:101158/136‑1 (MQ=255)                                 aCTTAATGTCCTTCGGAACGATCGGGCTTATTAAAGGAATTGAAAGCTATTCCGCTGGAAAAGGGACATAGCTGGCGACGTATGCAGCGAGGTGTATTGAAAATGAGATTGTAATTACAAAAGGGGGGTGCATACAccc                                                                                          <  8:66349/139‑1 (MQ=255)                                 aCTTAATCTCCATCGTAACGAGCGGGCTTATTAAAGGAAGTGAAAGCTAATCCGCTGGAAAAGGGACACCGCTGGCGACGTATGCAGCGAGGTGTATTGAAAATGAGATTGTAATTACAAAAGGGGGGTGCATacacgc                                                                                          <  6:211381/139‑3 (MQ=255)                                 aCTTAATCTCCATCGGAACGATCGGGCTTATTAAAGGAATTGAAAGCTATTCCGCTGGAAAAGGGACAAAGCTGGCGACGTATGCAGCGAGGTGTATTGAAAATGAGATTGTAATTACAAAAGGGGGGTGCATACAccc                                                                                          <  6:212446/139‑1 (MQ=255)                                 aCTTAATCTCCATCGGAACGAGCGGGCGTATTAAAGGAATTGAAAGCTATTCCGCTGGAAAAGGGACAAAGCTGGCGACGTATGCAGCGAGGTGTATTGAAAATGAGATTGTAATTACAAAAGGGGGGTGCATACAccc                                                                                          <  7:27919/139‑1 (MQ=255)                                   ttAATCTCCATCGGAACGATCGGGCTTATTAAAGGAATTGAAAGCTATTCCGCTGGAAAAGGGACAAAGCTGGCGACGTATGCAGCGAGGTGTATTGAAAATGAGATTGTAATTACAAAAGGGGGGTGCATACACCCct                                                                                        >  2:300485/1‑139 (MQ=255)                                     aaTCTCCATCGGAACGATCGGGCTTATTAAAGGAATTGAAAGCTATTCCGCTGGAAAAGGGACAAAGCTGGCGACGTATGCAGCGAGGTGTATTGAAAATGAGATTGTAATTACAAAAGGGGGGTGCATACACCCctct                                                                                      >  1:241948/1‑139 (MQ=255)                                           cATCGGAACGATCGTGCTTATTAAAGGAATTGAAAGCTATTCCGCTGGAAAAGGGACAAAGCTGGCGACGTATGCAGCGAGGTGTATTGAAAATGAGATTGTAATTa                                                                                                                <  6:288635/107‑1 (MQ=255)                                           cATCGGAACGATCGGGCTTATTAAAGGAATTGAAAGCTATTCCGCTGGAAAAGGGACAAAGCTGGCGACGTATGCAGCGAGGTGTATTGAAAATGAaatcctcatgcatttgcgcgcattgaaaaaaacaaaaaaagac                                                                                >  3:5624‑M1/1‑96 (MQ=255)                                           cATCGGAACGATCGGGCTTATTAAAGGAATTGAAAGCTATTCCGCTGGAAAAGGGACAAAGCTGGCGACGTATGCAGCGAGGTGTATTGAAAATGAGATTGTaa                                                                                                                   <  6:145832/104‑1 (MQ=255)                                           cATCGGAACGATCGGGCTTATTAAAGGAATTGAAAGCTATTCCGCTGGAAAAGGGACAAAGCTGGCGACGTATGCAGCGAGGTGTATTGAAAATGAGATTGTaa                                                                                                                   >  5:145832/1‑104 (MQ=255)                                           cATCGGAACGATCGGGCTTATTAAAGGAATTGAAAGCTATTCCGCTGGAAAAGGGACAAAGCTGGCGACGTATGCAGCGAGGTGTATTGAAAATGAGATTGTAATTa                                                                                                                >  5:288635/1‑107 (MQ=255)                                           cATCGGAACGATCGGGCTTATTAAAGGAATTGAAAGCTATTCCGCTGGAAAAGGGACAAAGCTGGCGACGTATGCAGCGAGGTGTATTGAAAATGAGATTGTAATTACAAAAGGGGGGTGCATACAccc                                                                                          <  8:108416/129‑1 (MQ=255)                                           cATCGGAACGATCGGGCTTATTAAAGGAATTGAAAGCTATTCCGCTGGAAAAGGGACAAAGCTGGCGACGTATGCAGCGAGGTGTATTGAAAATGAGATTGTAATTACAAAAGGGGGGTGCATACAccc                                                                                          >  7:108416/1‑129 (MQ=255)                                             tCGGAACGATCGGGCTTATTAAAGGAATTGAAAGCTATTCCGCTGGAAAAGGGACAAAGCTGGCGACGTATGCAGCGAGGTGTATTGAAAATGAGATTGTAATTACAAAAggggg                                                                                                      >  4:127353/1‑115 (MQ=255)                                             tCGGAACGATCGGGCTTATTAAAGGAATTGAAAGCTATTCCGCTGGAAAAGGGACAAAGCTGGCGACGTATGCAGCGAGGTGTATTGAAAATGAGATTGTAATTACAAAAggggg                                                                                                      <  3:127353/115‑1 (MQ=255)                                                gAACGATCGGGCTTATTAAAGGAATTGAAAGCTATTCCGCTGGAAAAGGGACAAAGCTGGCGACGTATGCAGCGAGGTGTATTGAAAATGAGATTGTAATTACAAAAGGGGGGTGCATACACCCCTCTTTAATACGttt                                                                           <  8:253516/139‑1 (MQ=255)                                                gAACGATCGGGCTTATTAAAGGAATTGAAAGCTATTCCGCTGGAAAAGGGACAAAGCTGGCGACGTATGCAGCGAGGTGTATTGAAAATGAGATTGTAATTACAAAAGGGGGGTGCATACACCCCTCTTTAATACGttt                                                                           <  7:224322/139‑1 (MQ=255)                                                 aaCGATCGGGCTTTTTAAAGGAATTGAAAGCTATTCCGCTGGAAAAGGGACAAAGCTGGCGACGTATGCAGCGAGGTGTATTGAAAATGAGATTGTAATTACAAAAGGGGGGTGCATACACCCCTCTTTAATACGTTTc                                                                          <  4:215471/139‑1 (MQ=255)                                                 aaCGATCGGGCTTATTAAAGGAATTGAAAGCTATTCCGCTGGAAAAGGGACAAAGCTGGCGACGTTTGCAGCGAGGTGTATTGAAAATGAaatcctcatgcatttgcgcgcatggaaaaaaacaaaaaaagacgtctcc                                                                          >  2:93668‑M1/1‑90 (MQ=255)                                                 aaCGATCGGGCTTATTAAAGGAATTGAAAGCTATTCCGCTGGAAAAGGGACAAAGCTGGCGACGTATGCAGCGAGGTGTATTGAAAATGAGATTGTAATTACAAAAGGGGGGTGCATACACCCCTCTTTAATACGTTTc                                                                          <  2:37591/139‑1 (MQ=255)                                                 aaCGATCGGGCATATTAAAGGAATTGAAAGCTATTACGCTGGAAACGGGACATAGCTGGCGACGTATGCAGCGTGGTGTATTGAAAATGAGATTGTAATTACAAAAGGGGGGTGCATACACCCCTCTTTAATACGTTTc                                                                          <  4:261313/139‑1 (MQ=255)                                                        gggCTTATTAAAGGAATTGAAAGCTATTCCGCTGGAAAAGGGACAAAGCTGGCGACGTATGCAGCGAGGTGTATTGAAAATGAGATTGTAATTACAAAAGGGGGGTGCATACACCCCTCTTTAATACGTTTCAatata                                                                    <  1:300485/138‑1 (MQ=255)                                                              attaAAGGAATTGAAAGCTATTCCGCTGGAAAAGGGACAAAGCTGGCGACGTATGCAGCGAGGTGTAGTGAACATGAGATTGTAATTACAAAAGGGGGGTGCATACACCCCTCTTTAATACGTTTCAATATATATGgtg                                                             >  7:174981/1‑139 (MQ=255)                                                                        ttGAAAGCTATTCCGCTGGATAAGGGACAAAGCTGGCGACGTATGCAGCGAGGTGTATTGAAAATGAGATTGTAATTACAAAAGGGGGGTGCATACACCCCTCTTTAATACGTTTCAATATATATGGTGTCAGAATCca                                                   >  1:307287/1‑139 (MQ=255)                                                                        ttGAAAGCTATTCCGCTGGAAAAGGGACAAAGCTGGCGACGTATGCAGCGAGGTATATTGAAAATGAGATTGTAATTACAAAAGGGGGGTGCATACACCCCTCTTTAATACGTTTCAatata                                                                    <  3:71142/122‑1 (MQ=255)                                                                        ttGAAAGCTATTCCGCTGGAAAAGGGACAAAGCTGGCGACGTATGCAGCGAGGTATATTGAAAATGAGATTGTAATTACAAAAGGGGGGTGCATACACCCCTCTTTAATACGTTTCAatata                                                                    >  4:71142/1‑122 (MQ=255)                                                                           aaaGCTATTCCGCAGGAAACGGGACAAACCTGGCGACGTATGCAGCGAGGTGTATTGAAAATGAGATTGTAATTACAAAAGGGGGGTGCATACACCCCTCTTTAATACGTTTCAATATATATGGTGTCAGAATCcaca                                                 <  1:74675/138‑1 (MQ=255)                                                                                  ttCCGCTGGAAAAGGGACAAAGCTGGCGACGTATGCAGCGAGGTGTATTGAAAATGAGATTGTAATTACAAAAGGGGGGTGCATACACCCCTCTTTAATACGTTTCAATATATATGGTGTCAGAATCCACAATGGTAAc                                         >  6:257777/1‑139 (MQ=255)                                                                                          gAAAAGGGACAAAGCTGGCGACGTATGCAGCGAGGTGTATTGAAAATGAaaacctcatgcatttgcgcgcattgaaaaaaacaaaaaaagacgtctcccttcacgatccgatcgggcaggataaggaagggaatgaaa                                  >  7:272541‑M1/1‑49 (MQ=255)                                                                                            aaaGGGACAAAGCTGGCGACGTATGCAGCGAGGTGTATTGAAAATGAGATTGTAATTACAAAAGGGGGGTGCATACACCCCTCTTTAATACGTTTc                                                                          <  7:84658/96‑1 (MQ=255)                                                                                            aaaGGGACAAAGCTGGCGACGTATGCAGCGAGGTGTATTGAAAATGAGATTGTAATTACAAAAGGGGGGTGCATACACCCCTCTTTAATACGTTTc                                                                          >  8:84658/1‑96 (MQ=255)                                                                                               gggACAAAGCTGGCGACGTATGCAGCGAGGTGTATTGAAAATGAGATTGTAATTACAAAAGGGGGGTGCATACACCCCTCTTTAATACGTTTCAATATATATGGTGTCAGAATCCACAATGGTAACTTCTTTCACGATa                            <  5:217533/139‑1 (MQ=255)                                                                                                  aCAAAGCTGGCGACGTATGCAGCGAGGTGTATTGAAAATGAGATTGTAATTACAAAAggggg                                                                                                      <  8:194955/62‑1 (MQ=255)                                                                                                  aCAAAGCTGGCGACGTATGCAGCGAGGTGTATTGAAAATGAGATTGTAATTACAAAAggggg                                                                                                      >  7:194955/1‑62 (MQ=255)                                                                                                    aaaGCTGGCGACGTATGCAGCGAGGTGTATTGAAAATGAGATTGTAATTACAAAAGGGGGGTGCATACACCCCTCTTTAATACGTTTCAATATATATGGTGTCAGAATCCACAATGGTAACTTCTTTCACGATAAAGt                        >  8:56854/1‑138 (MQ=255)                                                                                                           gCGACGTATGCAGCGAGGTGTATTGAAAATGAGATTGTAATTACAAAAgg                                                                                                         >  4:102041/1‑50 (MQ=255)                                                                                                           gCGACGTATGCAGCGAGGTGTATTGAAAATGAGATTGTAATTACAAAAgg                                                                                                         <  3:102041/50‑1 (MQ=255)                                                                                                           gCGACGTATGCAGCGAGGTGTATTGAAAATGAGATTGTAATTACAAAAGGGGGGTGCATACACCCCTCTTTAATACGTTTCAATATATATGGTGTCAGAATCCACAATGGTAACTTCTTTCACGATAAAGTTAACAAtt                >  7:226699/1‑139 (MQ=255)                                                                                                           gCGACGTATGCAGCGAGGTGTATTGAAAATGAGATTGTAATTACAAAAGGGGGGTGCATACACCCCTCTTTAATACGTTTCAATATATATGGTGTCAGAATCCACAAGGGTAACTTCTTTCACGATAAAGTTAACAAtt                >  1:102958/1‑139 (MQ=255)                                                                                                              aCGTATGCAGCGAGGTGTATTGAAAATGAGATTGTAATTACAAAAGGGGGGTGCATACACCCCTCTTTAATACGTTTCAATATATATGGTGTCAGAATCCACAATGGTAACTTCTTTCACGATAAAGTTAACAATTGtt             >  8:204519/1‑139 (MQ=255)                                                                                                                         gAGGTGTATTGAAAATGAGATTGTAATTACAAAAGGGGGGTGCATACACCCCTCTTTAATACGTTTCAATATATATGGTGTCAGAATCCACAATGGTAACTTCTTTCACGATAAAGTTAACAATTGTTTTTTTATCTTc  <  8:226699/139‑1 (MQ=255)                                                                                                                                          |                                                                                                                          AAAGTTCGAAAATACAGGTGAGGATGCAGAGGACTTAATCTCCATCGGAACGATCGGGCTTATTAAAGGAATTGAAAGCTATTCCGCTGGAAAAGGGACAAAGCTGGCGACGTATGCAGCGAGGTGTATTGAAAATGAGATTGTAATTACAAAAGGGGGGTGCATACACCCCTCTTTAATACGTTTCAATATATATGGTGTCAGAATCCACAATGGTAACTTCTTTCACGATAAAGTTAACAATTGTTTTTTTATCTTC  >  BS168_reference_genome/2653196‑2653454

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.