BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	pSIJ663_pHT315_araE_xy	2,206	C→A	9.6%	intergenic (–/‑772)	– / → eryR	–/–

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	pSIJ663_pHT315_araE_xy	2,206	0	C	A	9.6%	42296.1 / inf	13992	intergenic (–/‑772)	–/eryR	–/–
Reads supporting (aligned to +/- strand): ref base C (5329/7065); new base A (598/775); total (6028/7964)
Fisher's exact test for biased strand distribution p-value = 7.09e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.47e-02

Only 100 of 14345 total aligned reads displayed.

GTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATC‑TTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGAATAATTGATGGTATAAGGGACTTTCATGCTTGGAAAGTGGGGATTATG  >  pSIJ663_pHT315_araE_xy/2067‑2344

   cTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTTTCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCCT                                                                                                                                           >  6:71076‑M1/1‑139 (MQ=255)

          aaTCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑‑TTTTTTTATTAGCCGTCAAATTTCTCCCTCAtctctt                                                                                                                                   <  3:195751‑M1/139‑7 (MQ=255)

          aaTCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑‑TTTTTTTATTAGCCGTCAAATTTCTCCCTCAtctcgt                                                                                                                                   <  8:76479‑M1/139‑7 (MQ=255)

          aaTCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑‑TTTTTTTATTAGCCGTCAAATTTCTCCCTCAtctcgt                                                                                                                                   <  8:311767‑M1/139‑7 (MQ=255)

          aaTCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑‑TTTTTTTATTAGCCGTCAAATTTCTCCCTCAtctcgt                                                                                                                                   <  4:188562‑M1/139‑7 (MQ=255)

           aTCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCAtctcgt                                                                                                                                   <  5:216513‑M1/139‑7 (MQ=255)

           aTCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCATCCTCAtctcgt                                                                                                                                   <  1:93749‑M1/139‑7 (MQ=255)

            tCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTGTATTTACAATCTTTTTTTTTATTAGCCGTCAAATTTCTCCCTCAtctcgt                                                                                                                                   <  5:125745‑M1/139‑7 (MQ=255)

                ggAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCtt                                                                                                                              >  2:66140/1‑139 (MQ=255)

                ggAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCtt                                                                                                                              >  2:256915/1‑139 (MQ=255)

                ggAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑‑TTTTTTTATTAGACGTCAAATTTCTCCCTCATCTCGTCTCttt                                                                                                                             <  6:128022/139‑1 (MQ=255)

                  aaaCATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTTCCTCATCTCGTCTCTTta                                                                                                                            <  1:70321/139‑1 (MQ=255)

                              aTCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGCAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTT‑ATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATC‑TTTAtttta                                                                                                              >  6:49261‑M2/1‑134 (MQ=255)

                               tCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAATAGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATC‑TTTAtttta                                                                                                              >  3:31722‑M2/1‑134 (MQ=255)

                                cGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATC‑TTTAttttat                                                                                                             <  5:149766‑M2/139‑7 (MQ=255)

                                cGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCGTTATAGC‑TTTAgttta                                                                                                              <  8:108877‑M2/138‑6 (MQ=255)

                                  ttAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATA‑gttattttatgg                                                                                                           >  2:303530/1‑137 (MQ=255)

                                  ttAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCACTCATATCGTCTCTTTATATA‑TTTATTTTATCa                                                                                                           >  2:277769/1‑139 (MQ=255)

                                  ttAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑‑TTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATC‑TTTATTTTATCa                                                                                                           >  2:115327/1‑138 (MQ=255)

                                  ttAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTCGAAGTTTTGCCACAACAGTTTGTTTATTTACAATC‑‑TTTTTGTATTAGCCGTCAAATTTCTCCCTCATCTCGTGTCTTTATATG‑TTTATTTTATCa                                                                                                           >  6:173108/1‑138 (MQ=255)

                                   tAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATC‑TTTATTTTATCa                                                                                                           >  5:196109/1‑138 (MQ=255)

                                     gAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATC‑TTTAttttatcgta                                                                                                         >  1:21771‑M2/1‑128 (MQ=255)

                                       aGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑‑TTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATC‑TTTATTTTATCATAAAgg                                                                                                     <  1:169754/139‑1 (MQ=255)

                                        gTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATC‑TTTATTTTATCATGAAgg                                                                                                     <  2:139511/139‑1 (MQ=255)

                                        gTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATC‑TTTATTTTATCATAAAgg                                                                                                     <  7:204449/139‑1 (MQ=255)

                                        gTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATC‑TTTATTTTATCATAAAgg                                                                                                     <  4:255593/139‑1 (MQ=255)

                                        gTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCACTCATCTCGTCTCTTTATATC‑TTTATTTTATCATAAAgg                                                                                                     <  6:286492/139‑1 (MQ=255)

                                        gTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCACTCATCTCGTCTCTTTATATC‑TTTATTTTATCATAAAgg                                                                                                     <  8:55060/139‑1 (MQ=255)

                                         tGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCACTCATCTCGTCTCTTTATATC‑TTTATTTTATCATAAAGGa                                                                                                    <  1:282024/139‑1 (MQ=255)

                                           caagtcaagtTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAATAGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATC‑TTTATTTTATCATAAAGGAGt                                                                                                  >  2:236575/1‑139 (MQ=255)

                                           caagtcaagtTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATC‑TTTATTTTATCATAAAGGAg                                                                                                   >  5:82925/1‑138 (MQ=255)

                                           caagtcaagtTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCACTCATCTCGTCTCTTTATATC‑TTTATTTTATCATAAAgg                                                                                                     <  3:268164/136‑1 (MQ=255)

                                             agtcaagtTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCCAATTTCTCCCTCATCTCGTCTCTTTATATC‑TTTATTTTATCATAAAGGAGTAt                                                                                                >  4:27747/1‑139 (MQ=255)

                                                   gtTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTTAAATTTCTCCCTCATCTCGTCTCTTTATATC‑TTTATTTTATCATAAAGGAGTATTTGAAc                                                                                          >  4:208489/1‑139 (MQ=255)

                                                        atGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCAtctcg                                                                                                                                    <  7:136058‑M1/93‑6 (MQ=255)

                                                         tGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑‑TTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATC‑TTTATTTTATCATAAAGGAGTATTTGAACCGTcgcg                                                                                   <  3:99085/139‑1 (MQ=255)

                                                         tGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑‑TTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATC‑TTTATTTTATCATAAAGGAGTATTTGAACCGTcgcg                                                                                   <  1:100020/139‑1 (MQ=255)

                                                           tATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGTTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCgtctctttatatctttattttatcataaaggagtatttgaaccgtcgcgcgggac                                                                                   <  7:181603‑M1/139‑56 (MQ=255)

                                                           tATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATC‑TTTATTTTATCGTAAAGGAGTATTTGAACCGTcgcg                                                                                   <  5:23549/138‑1 (MQ=255)

                                                           tATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATC‑TTTATTTTATCGTAAAGGAGTATTTGAACCGTcgcg                                                                                   <  4:167344/138‑1 (MQ=255)

                                                             tCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCGCTCATCTCGTCTCTTTATATC‑TTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCgg                                                                                >  5:301539/1‑139 (MQ=255)

                                                             tCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATC‑TTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCgg                                                                                >  7:113979/1‑139 (MQ=255)

                                                             tCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATC‑TTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCgg                                                                                >  6:261971/1‑139 (MQ=255)

                                                             tCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑‑TTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATC‑TTTATTTTATCATAAAGGAGTATTTGAACCg                                                                                        <  4:120319/130‑1 (MQ=255)

                                                             tCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑‑TTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATC‑TTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCgg                                                                                >  1:4627/1‑138 (MQ=255)

                                                              cTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑‑TTTTTTTATTAGCCGTCAAAATTCTCCCTCATCTCGTCTCTTTATATC‑GTTATTTTAAAATAAATAAGTATTTTAAACATAGCGCgggg                                                                              >  6:198950/1‑138 (MQ=255)

                                                                 tactGATTTGTGGAAGTTTTGCCACAACAGTTTGGTTATTTACAATC‑TTTTTTTTATTAGCCGTCTAATTTCTCCCTCATCTCGTCGCTTTATATC‑TTTATTTTATTATAAAGGAGTATTTGAACCGTCGCGCGGGa                                                                              <  6:91463/134‑1 (MQ=255)

                                                                 taATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTTAAATTTCTCCCTCATCTCGTCTCTTTATATC‑TTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGa                                                                              >  2:163241/1‑137 (MQ=255)

                                                                 taATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATC‑TTTAtttta                                                                                                              >  5:277469‑M2/1‑100 (MQ=255)

                                                                 taATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATC‑TTTATTTTGTCATAAAGGAGTATTTGAACCGTCGCGCGGGa                                                                              <  7:157273/137‑1 (MQ=255)

                                                                 taATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATC‑TTTATTTTATCGTAAAGGAGTATTTGAACCGTCGCGCGGGa                                                                              <  8:292412/137‑1 (MQ=255)

                                                                 taATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATC‑TTTATTTTATCATa                                                                                                         <  7:293866/110‑1 (MQ=255)

                                                                 taATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATC‑TTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGa                                                                              <  7:225212/137‑1 (MQ=255)

                                                                 taATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCACTCATCTCGTCTCTTTATATC‑TTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGa                                                                              <  8:247213/137‑1 (MQ=255)

                                                                 taATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATCTCTCCCTCATCTCGTCTCTTTATATC‑TTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGa                                                                              >  2:43729/1‑137 (MQ=255)

                                                                 taATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑‑TTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATC‑TTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGa                                                                              <  5:104302/136‑1 (MQ=255)

                                                                 taATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑‑TTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATC‑TTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGa                                                                              <  8:177304/136‑1 (MQ=255)

                                                                 taATGATTTGTGGAAGTTTTGCCACAACAGTTGGTGTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTTTTTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGa                                                                              <  7:32815/138‑1 (MQ=255)

                                                                 taATGATTAGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCACTCATCTCGTCTCTTTATATC‑TTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGa                                                                              <  1:146727/137‑1 (MQ=255)

                                                                   aTGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑‑TTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATC‑TTTATTTTATCATAAAgg                                                                                                     <  3:77508/111‑1 (MQ=255)

                                                                      aTTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCAtctcgt                                                                                                                                   <  5:255987‑M1/80‑7 (MQ=255)

                                                                      aTTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCAtctcgt                                                                                                                                   >  8:268653‑M1/1‑74 (MQ=255)

                                                                      aTTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCACTCATCTCGTCTCTTTATATC‑TTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGtt                                                                        >  6:240333/1‑138 (MQ=255)

                                                                      aTTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTT‑ATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATC‑TTTAttt                                                                                                                >  2:85497‑M2/1‑94 (MQ=255)

                                                                        tTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑‑TTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATC‑TTTATTTTATCATAAAGGAGTATTTGAAACGTCGCGCGGGACCGGTTTAt                                                                     >  5:39204‑M2/2‑138 (MQ=255)

                                                                         tgtgGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCAtctcgt                                                                                                                                   <  6:151402‑M1/77‑7 (MQ=255)

                                                                         tgtgGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATC‑TTTGTTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATg                                                                    >  8:129340/1‑139 (MQ=255)

                                                                         tgtgGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATC‑TTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATg                                                                    >  7:82241/1‑139 (MQ=255)

                                                                         tgtgGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑‑TTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATC‑TTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGa                                                                   >  3:56559/1‑139 (MQ=255)

                                                                           tgGAAGTTTTGCCACAATAGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATC‑TTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGa                                                                              >  1:195189/1‑127 (MQ=255)

                                                                           tgGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATC‑TTTTTTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGAt                                                                  >  2:210885/1‑139 (MQ=255)

                                                                           tgGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATC‑TTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGa                                                                              >  8:152289/1‑127 (MQ=255)

                                                                           tgGAAGTTTTGCCACAACAGTTGGTTTATATACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATA‑TTTATTATAAAATAAAGGAGTAATTGAACCTTCGCCCGGGACAGGTTTAGGa                                                                   >  5:62192/1‑138 (MQ=255)

                                                                           tgGAAGTTTTGCCACAACAGTTGGTTTAATTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATC‑TTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGAt                                                                  <  6:105138/139‑1 (MQ=255)

                                                                           tgGAAGTTTTGACAAAACAGTTTGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATC‑TTTATTTTGTCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGAt                                                                  <  6:219504/139‑1 (MQ=255)

                                                                             gAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑‑TTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATC‑TTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAg                                                                <  3:132206/138‑1 (MQ=255)

                                                                              aaGTTTTGCCACAACAGTTGGTTTATTTACAATC‑‑TTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATC‑TTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAggg                                                              <  4:143064/139‑1 (MQ=255)

                                                                               aGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATC‑TTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAggg                                                              <  1:240988/139‑1 (MQ=255)

                                                                               aGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATC‑TTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAggg                                                              <  1:306967/139‑1 (MQ=255)

                                                                                gTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATC‑TTTATTTTGTCATAAAGGAGTATTTGAACCGTCGCGCGGGAc                                                                             >  3:291246/1‑123 (MQ=255)

                                                                                gTTTTGCCACAACAGTTGGTTTATTTACAATC‑‑TTTTTTTATTAGCCGTCAAATTTCTTCCTCATCTCGTCTCTTTATATG‑TTTATTTTATCATAAAGGAGTATTTGAACAGTCGCGCGGGACAGGTTTATGATAGGGa                                                             >  4:298219/1‑138 (MQ=255)

                                                                                       cacaACAGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATC‑TTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTa                                                      <  8:220331/139‑1 (MQ=255)

                                                                                       cacaACAGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATC‑TTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTa                                                      <  1:260502/139‑1 (MQ=255)

                                                                                                  gTTTATTTACAATC‑TTTTTTTTATTAGCCGTCATATTTCTCACTCATCTCGTCTCTTTATATG‑TTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGAATAAtt                                            <  4:37902/138‑1 (MQ=255)

                                                                                                  gTTTATTTACAATC‑‑TTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATC‑TTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGAATAATTGa                                          <  7:249427/139‑1 (MQ=255)

                                                                                                   tttatttaCAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCGTCTCGTCTCTTTATATC‑TTTATTTTATCATAAAGGAGTATTTGTACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGAATAATTGa                                          <  1:12231/139‑1 (MQ=255)

                                                                                                   tttatttaCAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCGTCTCGTCTCTTTATATC‑TTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGAATAATTGa                                          <  3:217133/139‑1 (MQ=255)

                                                                                                   tttatttaCAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATC‑TTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGAATAATTGa                                          <  5:171866/139‑1 (MQ=255)

                                                                                                   tttatttaCAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATC‑TTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGAATAATTGa                                          <  3:172614/139‑1 (MQ=255)

                                                                                                   tttatttaCAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATC‑TTTATTTTATCATAAAGGAGTATTTGAACCGGCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGAATAATTGa                                          <  3:241591/139‑1 (MQ=255)

                                                                                                   tttatttaCAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCACTCATCTCGTCTCTTTATATC‑TTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGAATAATTGa                                          <  3:152566/139‑1 (MQ=255)

                                                                                                    ttatttaCAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCGCTCATCTCGTCTCTTTATATC‑TTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGAATAATTGAt                                         >  3:111074/1‑139 (MQ=255)

                                                                                                       tttaCAATC‑‑TTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATC‑TTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGAATAATTGATGGta                                     <  4:81186/139‑1 (MQ=255)

                                                                                                             caatcTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATC‑TTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGAATAATTGATGGTATAAg                                 >  6:307968‑M1/6‑139 (MQ=255)

                                                                                                             ATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTTGTCTCTTTATATC‑TTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGAATAATTGATGGTATAAgg                                >  7:270785‑M1/1‑139 (MQ=255)

                                                                                                             ATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATC‑TTTATTTTATTATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGAATAATTGATGGTATAAgg                                >  7:59054‑M1/1‑139 (MQ=255)

                                                                                                                     TTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATC‑TTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGAATAATTGATGGTATAAGGGACttt                          >  4:229139‑M1/1‑138 (MQ=255)

                                                                                                                     TTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATC‑TTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGAATAATTGATGGTATAAGGGACttt                          >  4:61062‑M1/1‑138 (MQ=255)

                                                                                                                                     aTTTCTCCCTCATCTCGTCTCTTTATATC‑TTTATTTTATCGTAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGAATAATTGATGGTATAAGGGACTTTCATGCTTGGAAAGTggg         <  2:190148/139‑1 (MQ=255)

                                                                                                                                            ccTCATCTCGTCTCTTTATATC‑TTTATTTTATCGTAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGAATAATTGATGGTATAAGGGACTTTCATGCTTGGAAAGTGGGGATTa    <  8:20003/137‑1 (MQ=255)

GTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATC‑TTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGAATAATTGATGGTATAAGGGACTTTCATGCTTGGAAAGTGGGGATTATG  >  pSIJ663_pHT315_araE_xy/2067‑2344

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`