BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	pSIJ663_pHT315_araE_xy	2,239	A→G	7.4%	intergenic (–/‑739)	– / → eryR	–/–

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	pSIJ663_pHT315_araE_xy	2,239	0	A	G	7.4%	39140.9 / inf	13047	intergenic (–/‑739)	–/eryR	–/–
Reads supporting (aligned to +/- strand): ref base A (5011/6980); new base G (392/570); total (5471/7576)
Fisher's exact test for biased strand distribution p-value = 5.41e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 13182 total aligned reads displayed.

GTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGAATAATTGATGGTATAAGGGACTTTCATGCTTGGAAAGTGGGGATTATGAATTAGATGCTTGTCCACAATATGTTCCAATG  >  pSIJ663_pHT315_araE_xy/2100‑2376

 ttAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTGTCGTTATTTCTTTATTTTATCa                                                                                                                                           >  3:170096/1‑139 (MQ=255)

 ttAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCACTCATCTCGTCTCTTTATATCTTTATTTTATCa                                                                                                                                           >  4:66231/1‑139 (MQ=255)

      aGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAgg                                                                                                                                     <  5:184386/139‑1 (MQ=255)

       gTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCACTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAgg                                                                                                                                     <  4:217985/139‑1 (MQ=255)

       gTGCAAGTCAAGGTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAACATTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAgg                                                                                                                                     <  7:103115/139‑1 (MQ=255)

          caagtcaagtTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATGAAgg                                                                                                                                     >  8:293344/1‑136 (MQ=255)

          caagtcaagtTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAg                                                                                                                                   >  6:100108/1‑138 (MQ=255)

          caagtcaagtTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATa                                                                                                                                         >  8:155476/1‑131 (MQ=255)

                  gtTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAAc                                                                                                                          <  3:94318/139‑1 (MQ=255)

                       atGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTcg                                                                                                                     >  5:29499/1‑139 (MQ=255)

                       atGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCACTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTcg                                                                                                                     >  8:179524/1‑139 (MQ=255)

                        tGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTcgcg                                                                                                                   <  1:100020/139‑1 (MQ=255)

                          tATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTcgcg                                                                                                                   <  1:257137/138‑1 (MQ=255)

                            tCTATAATGATTTGTGGAAGTTTTGCCACAATAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCgg                                                                                                                >  1:276575/1‑139 (MQ=255)

                            tCTATAATGATTTGTGGAAGTTTTGCCACAATAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCGTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCgg                                                                                                                >  6:22053/1‑139 (MQ=255)

                            tCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCGTCTAGTCTCTTTATATATTTATTTTATAATAAACGAGTATTTTATCCGTCGCGCgg                                                                                                                >  8:270638/1‑139 (MQ=255)

                            tCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTTTTTTTTCATAAAGGAGTATTTGAACCGTAGCGCgg                                                                                                                >  6:36092/1‑139 (MQ=255)

                            tCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCGTAAAGGAGTATTTGAACCGTCGCGCgg                                                                                                                >  6:80702/1‑139 (MQ=255)

                            tCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCGTAAAGCTGTATTTTAACCGTCGCGAgg                                                                                                                >  5:161000/1‑139 (MQ=255)

                            tCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCACTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGTAGTATTTGAACCGACGCGCgg                                                                                                                >  8:201369/1‑139 (MQ=255)

                            tCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCggg                                                                                                               >  2:20620/1‑139 (MQ=255)

                              tataATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGa                                                                                                              <  4:242366/139‑1 (MQ=255)

                                tctataatTGTGGAAGTTTTGCGACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTcgcg                                                                                                                   <  3:213109‑M2/124‑1 (MQ=255)

                                taATGCTTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTGTCATAAAGGAGTATTTGAACCGTCGCGCGGGa                                                                                                              <  2:131354/137‑1 (MQ=255)

                                taATGATTTGTGGAAGTTTTGCCACATCAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCCTCTCTTTATATCTTTATTTTATCGTAAAGGAGTATTTGAACCGTCGCGCGGGa                                                                                                              <  8:56585/137‑1 (MQ=255)

                                taATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCGTCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGa                                                                                                              <  7:85875/137‑1 (MQ=255)

                                taATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTGTCATAAAGGAGTATTTGAACCGTCGCGCGGGAc                                                                                                             <  4:44185/138‑1 (MQ=255)

                                taATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATGAAGGAGTATTTGAACCGTCGCGCGGGa                                                                                                              <  3:148599/137‑1 (MQ=255)

                                taATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTTAACCGTCGCGCGGGa                                                                                                              >  6:170600/1‑137 (MQ=255)

                                taATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGa                                                                                                              <  5:113933/137‑1 (MQ=255)

                                taATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGAc                                                                                                             <  5:59784/138‑1 (MQ=255)

                                taATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCACTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGa                                                                                                              <  7:38020/137‑1 (MQ=255)

                                taATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCACTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGAc                                                                                                             <  1:170086/138‑1 (MQ=255)

                                taATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGa                                                                                                              <  2:81588/136‑1 (MQ=255)

                                taATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACa                                                                                                            <  1:302457/138‑1 (MQ=255)

                                taATGATTAGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCACTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGa                                                                                                              <  1:146727/137‑1 (MQ=255)

                                 aaTGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCGTAAAGGAGTATTTGAACCGTcgcg                                                                                                                   >  4:284701/1‑131 (MQ=255)

                                    gATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTATTAGCCGTCAAATTTCTCCCTCATCACGTCTCTTTATATCTTTATTTTATCATAAATGAGTATTTGAACCGTCGCGCGGGACAGGttt                                                                                                       >  2:40598/1‑139 (MQ=255)

                                     aTTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCGAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAgg                                                                                                                                     >  4:176576/1‑109 (MQ=255)

                                     aTTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTTATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGtt                                                                                                        >  5:272197/1‑138 (MQ=255)

                                     aTTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTTGTCTATTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGtt                                                                                                        <  5:226508/138‑1 (MQ=255)

                                      tttGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTGTCATAAAgg                                                                                                                                     <  4:305330/108‑1 (MQ=255)

                                      tttGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGaa                                                                                                                           <  7:254433/118‑1 (MQ=255)

                                       tTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTGTCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTAt                                                                                                     >  2:230147‑M2/2‑139 (MQ=255)

                                        tgtgGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATGAAGGAGTATTTGAACCGTCGCGCGGGAc                                                                                                             <  7:165460/130‑1 (MQ=255)

                                        tgtgGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTCATCATAAAGGAGTATTTGAACCGTCGCGCGGGAc                                                                                                             <  6:126357/130‑1 (MQ=255)

                                        tgtgGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCACTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTAt                                                                                                     <  4:113943/138‑1 (MQ=255)

                                        tgtgGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTT‑TT‑GCCGTGTAATTTGTCGCTCAGCTCGGGTCTGTATATATTTTTTTTTTATTATATTTGTTTTTTATCAGGCGCGCGGGGCAGGGTTTTGAt                                                                                                  >  1:86831/1‑139 (MQ=255)

                                          tgGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCACTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGa                                                                                                              >  1:195924/1‑127 (MQ=255)

                                          tgGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGAc                                                                                                             <  6:61649/127‑1 (MQ=255)

                                          tgGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGAt                                                                                                  <  6:196603/138‑1 (MQ=255)

                                          tgGAAGTTTAGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCACTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGAt                                                                                                  <  3:28176/139‑1 (MQ=255)

                                              aGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCACTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAggg                                                                                              <  7:209314/139‑1 (MQ=255)

                                                    gCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGAGAGGTTTATGATAGGGATAttt                                                                                        >  3:12663/1‑139 (MQ=255)

                                                    gCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATAtttt                                                                                       >  3:47388/1‑139 (MQ=255)

                                                      cacaACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCACTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTa                                                                                      <  7:299397/139‑1 (MQ=255)

                                                          acaGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGa                                                                                  <  7:143353/139‑1 (MQ=255)

                                                              ttGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGaataa                                                                              >  2:272153/1‑139 (MQ=255)

                                                                ggTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCACTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGaataat                                                                             <  8:13257/138‑1 (MQ=255)

                                                                 gTTTATTTACAATC‑TTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGAATAATTGa                                                                          <  4:90143/139‑1 (MQ=255)

                                                                 gTTTATTTACAATC‑TTTTATTATTAGCCGTCAAAATGATCCCTCATCTCGTCTATTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGAATAATTGa                                                                          <  2:156652/139‑1 (MQ=255)

                                                                  tttatttaCAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCGTAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGAATAATTGa                                                                          <  7:230813/139‑1 (MQ=255)

                                                                  tttatttaCAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGAATAATTGa                                                                          <  5:134214/139‑1 (MQ=255)

                                                                  tttatttaCAATCTTTTTTTTATTAGCCGTCAAATTTCTCACTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGAATAATTGa                                                                          <  4:198008/139‑1 (MQ=255)

                                                                  gttatttaCAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGAATAATTGa                                                                          <  2:184519/138‑1 (MQ=255)

                                                                  attatttaCAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGAATAATTGa                                                                          <  8:112309/138‑1 (MQ=255)

                                                                    tatttaCAATCTTTTTTTTATTAGCCGTCAAATTTCTCACTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGAATAATTGATg                                                                        >  1:63962/1‑139 (MQ=255)

                                                                          cAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGAATAATTGATGGtata                                                                   <  7:276750/138‑1 (MQ=255)

                                                                           aaTCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGAc                                                                                                             <  6:147561/95‑1 (MQ=255)

                                                                            aatcTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATATGGATATTTTATTGAATAATTGATGGTATAAgg                                                                <  2:25130‑M1/135‑1 (MQ=255)

                                                                            aatcTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGAATAATTGATGGTATAAgg                                                                <  6:294388‑M1/135‑1 (MQ=255)

                                                                            ATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCGTAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGTTATTTTATTGAATAATTGATGGTATAAgg                                                                <  3:260513‑M1/139‑1 (MQ=255)

                                                                             atcTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGAATAATTGATGGTATATggg                                                               <  2:110244‑M1/136‑1 (MQ=255)

                                                                                 TTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGAATAATTGATGGTATAAGGGACtt                                                           <  3:107539‑M1/139‑1 (MQ=255)

                                                                                   TTTTATTAGGCGTGAAATTTCTACCGCATCTCGTCTCTTTATATCTTTATTTTATAATTAAGGAGGATTTGAACCGTCGCGCGGGTCAGGTTTATGATAGGGATATTTTATTGAATAATTGATGGTATAAGGGACttt                                                          >  6:268800‑M1/1‑138 (MQ=255)

                                                                                     TTATTAGCCGTGAAATTTCTCACTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGAATATTTGATGGTATAAGGGACTTTCa                                                        >  5:79323‑M1/1‑138 (MQ=255)

                                                                                        TTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGa                                                                                                   <  5:207324‑M1/92‑1 (MQ=255)

                                                                                                 aaaTTTCTACTTCATCTCGTCTCTTTATATCTTTATTTTATCATAAATGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGAATAATTGATGGTATAAGGGACTTTCATGCTTGGAAAGt                                            <  6:243412/138‑1 (MQ=255)

                                                                                                  aaTTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATTAAGGAGTATTTTAACCGTCGCGCGGTACAGGTTTATGATAGGTATATTTTATTGAATAATTGATGGTATAAGGGACTTTCATGCTTGGAAAGTgg                                          <  3:236405/139‑1 (MQ=255)

                                                                                                              aTCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCTGGACAGGATTATGATAGGGATATTTTATTGAATAATTGATTGTATAAGGGACTTTCATGCTTGGAAAGTGGGGATTATGAAtt                              <  4:15663/139‑1 (MQ=255)

                                                                                                                    tctcTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGAATAATTGATGGTATAAGGGACTTTCATGCTTGGAAAGTGGGGATTATGAATTAGATGc                        <  5:36824/139‑1 (MQ=255)

                                                                                                                        tttATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGAATAATTGATGGTATAAGGGACTTTCATGCTTGGAAAGTGGGGATTATGAATTAGATGCtt                      <  8:223126/137‑1 (MQ=255)

                                                                                                                            tatcTTTATTTTATCATGAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGAATAATTGATGGTATAAGGGACTTTCATGCTTGGAAAGTGGGGATTATGAATTAGATGCTTGTCca                 <  7:124607‑M2/134‑1 (MQ=255)

                                                                                                                            tatcTTTATTTCATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGAATAATTGATGGTATAAGGGACTTTCATGCTTGGAAAGTGGGGATTATGAATTAGATGCTTGTCca                 <  5:248402‑M2/134‑1 (MQ=255)

                                                                                                                             atcTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGAATAATTGATGGTATAAGGGACTTTCATGCTTGGAAAGTGGGGATTATGAATTAGATGCTTGTCcac                >  2:29738‑M2/4‑138 (MQ=255)

                                                                                                                                tttattttatCATTAAGGTGTTTTTGAACCGTCGCGCGGGACAGGTTTATGATATGGATATTTTTTTGAATAATTGATGGTATAAGGGACTTTCATGCTTGGAAAGTGGGGATTATGAATTAGATGCTTGTCCACAata            <  8:31530/139‑1 (MQ=255)

                                                                                                                                 ttgttttatCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGAATAATTGATGGTATAAGGGACTTTCATGCTTGGAAAGTGGGGATTATGAATTAGATGCTTGTCCACAatat           >  7:188219/4‑139 (MQ=255)

                                                                                                                                  tattttatTATAAAGGAGTATTTGAACCGTCGCGCGGGACATGTTTATGATAGGGATATTTTATTGAATAATTGATGGTATAAGGGACTTTCATGCTTGGAAAGTGGGGATTATGAATTAGATGCTTGTCCACAatat           <  3:190699/138‑1 (MQ=255)

                                                                                                                                  tattttatCATAAAGGAGTATTTGACCCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGAATAATTGATGGTATAAGGGACTTTCATGCTTGGAAAGTGGGGATTATGAATTAGATGCTTGTCCACAat             <  8:77013/136‑1 (MQ=255)

                                                                                                                                  tattttatCATAAAGGAGTATTTGAACCGTCGCGGGGTACAGGTTTATGATAGGGATATTTTATTGATTAATTGAGGGTATAAGGGACTTTCATGCTTGGAAAGTGGGGTTTATGAATTAGATGCTTGTACAGAATATg          >  7:63628/1‑139 (MQ=255)

                                                                                                                                  tattttatCATAAAGGAGTATTTGAACCGTCGCGCGGGAc                                                                                                             >  1:4346/1‑40 (MQ=255)

                                                                                                                                  tattttatCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGaa                                                                                 <  3:305892/68‑1 (MQ=255)

                                                                                                                                  tattttatCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGAATAATTGATGGTATAAGGGACTTTCa                                                        >  1:238732/1‑93 (MQ=255)

                                                                                                                                  tattttatCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGAATAATTGATGGTATAAGGGACTTTCATGCTTGGaa                                               <  8:248412/102‑1 (MQ=255)

                                                                                                                                  tattttatCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGAATAATTGATGGTATAAGGGACTTTCATGCTTGGAAAGTGGGGATTa                                    >  1:21739/1‑113 (MQ=255)

                                                                                                                                  tattttatCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGAATAATTGATGGTATAAGGGACTTTCATGCTTGGAAAGTGGGGATTATGAATTAGATGc                        >  4:135042/1‑125 (MQ=255)

                                                                                                                                  tattttatCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGAATAATTGATGGTATAAGGGACTTTCATGCTTGGAAAGTGGGGATTATGAATTAGATGCTTGTCCACAat             <  3:70074/136‑1 (MQ=255)

                                                                                                                                  tattttatCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGAATAATTGATGGTATAAGGGACTTTCATGCTTGGAAAGTGGGGATTATGAATTAGATGCTTGTCCACAATATg          >  4:26443/1‑139 (MQ=255)

                                                                                                                                  tattttatCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGAATAATTGATGGTATAAGGGACTTTCATGCTTGGAAAGTGGGGATTATGAATTAGATGCTTGTCCACAATATg          >  1:121464/1‑139 (MQ=255)

                                                                                                                                         tCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGAATAATTGATGGTATAAGGGACTTTCATGCTTGGAAAGGGGGGATTATGAATTAGATGCTTGTCCACAATATGTTCCAAt   <  2:60108/139‑1 (MQ=255)

GTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGAATAATTGATGGTATAAGGGACTTTCATGCTTGGAAAGTGGGGATTATGAATTAGATGCTTGTCCACAATATGTTCCAATG  >  pSIJ663_pHT315_araE_xy/2100‑2376

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`