BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	pSIJ663_pHT315_araE_xy	3,729	T→G	7.2%	intergenic (+14/‑142)	eryR → / → ampR	–/–

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	pSIJ663_pHT315_araE_xy	3,729	0	T	G	7.2%	18688.7 / inf	6408	intergenic (+14/‑142)	eryR/ampR	–/–
Reads supporting (aligned to +/- strand): ref base T (494/5316); new base G (563/19); total (1058/5350)
Fisher's exact test for biased strand distribution p-value = 0.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 6737 total aligned reads displayed.

TGTTTACTAAAAATCAGTTTCATCAAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGTATCGGGGTACATTGAAAAAGGAAGAGTAT  >  pSIJ663_pHT315_araE_xy/3591‑3872

  tttACTAAAAATCAGTTTCATCAAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCttt                                                                                                                                               >  4:146341/1‑139 (MQ=255)

  tttACTAAAAATCAGTTTCATCAAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCttt                                                                                                                                               >  4:270052/1‑139 (MQ=255)

  tttACTAAAAATCAGTTTCATCAAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCttt                                                                                                                                               >  1:42711/1‑139 (MQ=255)

  tttACTAAAAATCAGTTTCATCAAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCtgt                                                                                                                                               >  1:228956/1‑137 (MQ=255)

  tttACTAAAAATCAGTTTCATCAAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCgtt                                                                                                                                               >  8:298330/1‑139 (MQ=255)

  tttACTAAAAATCAGTTTCATCAAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTAAACGGGAGGAAATAATTCTATGAGTCGCgtt                                                                                                                                               >  1:300863/1‑139 (MQ=255)

    tACTAAAAATCAGTTTCATCAAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCttttt                                                                                                                                             >  3:30037/1‑139 (MQ=255)

    tACTAAAAATCAGTTTCATCAAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTATATGAGTCGCgtttt                                                                                                                                             >  8:163650/1‑139 (MQ=255)

     aCTAAAAATCAGTTTCATCAAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCtttttt                                                                                                                                            >  6:289740/1‑139 (MQ=255)

            aTCAGTTTCATCAAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCGTTTTTTAAtttt                                                                                                                                     >  4:217093/1‑138 (MQ=255)

             tCAGTTTCATCAAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTgg                                                                                                                                    <  5:282660/139‑1 (MQ=255)

             tCAGTTTCATCAAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTgg                                                                                                                                    <  8:201621/139‑1 (MQ=255)

              cAGTTTCATCAAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGa                                                                                                                                   <  8:141119/139‑1 (MQ=255)

               aGTTTCATCAAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGa                                                                                                                                   <  3:210086/138‑1 (MQ=255)

               aGTTTCATCAAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGa                                                                                                                                   <  7:241659/138‑1 (MQ=255)

               aGTTTAATCAAGCAATGAAACACGGCTAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATTATTCTATGAGTCGGTTTTTTAAATTTGGa                                                                                                                                   <  7:98964/138‑1 (MQ=255)

                 tttCATCAAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAg                                                                                                                                <  1:185235/139‑1 (MQ=255)

                      tcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTaca                                                                                                                           <  4:169666/139‑1 (MQ=255)

                      tcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTaca                                                                                                                           <  7:157369/139‑1 (MQ=255)

                      tcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTaca                                                                                                                           <  6:238395/139‑1 (MQ=255)

                      tcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTaca                                                                                                                           <  2:277250/139‑1 (MQ=255)

                      tcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTaca                                                                                                                           <  1:163398/139‑1 (MQ=255)

                        aaGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGATCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACg                                                                                                                         <  7:200560/139‑1 (MQ=255)

                        aaGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACg                                                                                                                         <  2:224423/139‑1 (MQ=255)

                        aaGCAATGAAACACGCCAAAGTAAAAAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACg                                                                                                                         <  8:271793/139‑1 (MQ=255)

                             aTGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACt                                                                                                                    <  3:146341/139‑1 (MQ=255)

                             aTGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACt                                                                                                                    <  3:53234/139‑1 (MQ=255)

                             aTGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACt                                                                                                                    <  1:86014/139‑1 (MQ=255)

                                aaaCACGCCAAATTATAGAATTTAACTACCTTTACTTATGAGCTAGTATTGTCTATTTTTAATTGTTATCTATTATTTAACGTTATGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAATTTACACGTTACTaa                                                                                                                  <  8:117238/138‑1 (MQ=255)

                                aaaCACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTaaa                                                                                                                 <  7:58120/139‑1 (MQ=255)

                                aaaCACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTaaa                                                                                                                 <  1:274025/139‑1 (MQ=255)

                                     cGCCAAAGTAAACTTTTTAAGTTCCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTTACGGGAGGAAATAATTGTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGaa                                                                                                            <  5:198727/139‑1 (MQ=255)

                                           aGTAAACAATTTAAGTACAATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGATGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGgaga                                                                                                      <  6:310685/139‑1 (MQ=255)

                                             tAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATa                                                                                                    <  6:1444/139‑1 (MQ=255)

                                             tAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGCAAAGTTACACGTTACTAAAGGGAATGGAGATa                                                                                                    <  6:21616/139‑1 (MQ=255)

                                              aaaCAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATa                                                                                                    <  1:118972/138‑1 (MQ=255)

                                               atcAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTATCGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACAAGTTACTAAAGGGAATGGAGATaaa                                                                                                  <  2:311424/137‑1 (MQ=255)

                                               aaCAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATa                                                                                                    <  7:278431/137‑1 (MQ=255)

                                                  aaTTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTTTTATTTAACGGGAGGAAATAATTCTATGAGTCGCGTTTTTTTTTTTGGTAAGTTTACCGTTTCGTAAGGGGATGGGGATTAattt                                                                                               >  5:127342/1‑138 (MQ=255)

                                                    tttAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAattatt                                                                                             <  7:104203/139‑1 (MQ=255)

                                                    tttAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAattatt                                                                                             <  3:188810/139‑1 (MQ=255)

                                                    tttAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAattatt                                                                                             <  4:190905/139‑1 (MQ=255)

                                                    agacaGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAattatt                                                                                             <  5:7929/135‑1 (MQ=255)

                                                       aaGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAg                                                                                           <  4:119822/138‑1 (MQ=255)

                                                       aaGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAg                                                                                           <  7:37731/138‑1 (MQ=255)

                                                       aaGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAg                                                                                           <  6:166745/138‑1 (MQ=255)

                                                        aGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGat                                                                                         <  5:111448/139‑1 (MQ=255)

                                                            ccATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGAtata                                                                                      <  2:108346/138‑1 (MQ=255)

                                                            ccATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGAtata                                                                                      <  4:3640/138‑1 (MQ=255)

                                                              aTTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCGTTTTTAAATTTTGAAAGTTAAACGTTAATAAATGGAATGGAGATAAATTATtatatataat                                                                                    >  8:246371/1‑136 (MQ=255)

                                                                    tATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTTAATTTTGTAAATTTAACGTTAATAAAGGGAATGGTGATTAAATTTTATATATACTACTGACa                                                                             >  8:77131/1‑139 (MQ=255)

                                                                     aTGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGAc                                                                              <  5:221089/137‑1 (MQ=255)

                                                                     aTGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGAc                                                                              <  5:301515/137‑1 (MQ=255)

                                                                     aTGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGAc                                                                              <  2:136971/137‑1 (MQ=255)

                                                                        aGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCGGTTTTTTTTTTTGATTGTTAGACGGTACTAAAGGGAATGGGGATTAATTTTTTTATTTAATAATGACAGCtt                                                                         >  1:100323/1‑139 (MQ=255)

                                                                           aaGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCGTTTTTAAATTTTGAAATTTAGACGTTTCTAAAAGGCATTGAGATAAATTTTTATATATACTACTGAAAGCTTcc                                                                       >  4:91063/1‑138 (MQ=255)

                                                                             gTATTGTCTATTTTTATTAGTTATCTATTATTTTTCTGGAGGAAATTATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTTCTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTACaag                                                                    <  8:183018/139‑1 (MQ=255)

                                                                             gTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCaag                                                                    <  4:64160/139‑1 (MQ=255)

                                                                             gTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCaag                                                                    <  4:245920/139‑1 (MQ=255)

                                                                              tATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGGCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCaaga                                                                   <  5:37935/139‑1 (MQ=255)

                                                                                ttGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCaagaag                                                                 <  7:7662/139‑1 (MQ=255)

                                                                                ttGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCaagaag                                                                 <  7:220464/139‑1 (MQ=255)

                                                                                  gTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGc                                                                <  3:25966/138‑1 (MQ=255)

                                                                                   tCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTTAATTTTGTAATTTTAACGTTAATAAAGTGTATGGTGATAAATTTTTATATATAATAAATACAACTTCCAAGAAACt                                                               >  8:36252/1‑138 (MQ=255)

                                                                                   tCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCGTTTTTTAATTTGGGAAGGTAAACGGTACAAAAGGGAAAGGGGGTAAATTTTTTTATATACTACTTACAGCATGCAAGAAGcg                                                               >  8:223368/1‑137 (MQ=255)

                                                                                       tttttAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTaaa                                                            <  5:153644/137‑1 (MQ=255)

                                                                                        ttttAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCGTTTTTTAATTTTGAAATTTACACCTTAATAAAGGGAATTGAGATAAAATATTTTATATAAAAATTACACCTTACAACAAGCGAAagag                                                         >  6:53341/1‑139 (MQ=255)

                                                                                        ttttAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCGTTTTTTAATTTTGAAAGTTTCCCGTTACTAAAGGGAATGGTGATTAATTATTTGATATAATACAGACACCTTCCCAGAAGCTAAagag                                                         >  2:169664/1‑139 (MQ=255)

                                                                                         tttAATTGTTATCTATAATTTAACGGGAGGAAATATTTCTATGAGTCGATTTTTTAAATTTGTAAAGTTAAACGTTAAAAAAGTGAATGGATATAAATTTTAAGATATACTACTGACAGCTTCCAAGAAGCTAAacag                                                         <  1:142591/138‑1 (MQ=255)

                                                                                         tttAATAGTTATCTATTATTTAACGTTATGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAATTTAAACGTTAATAAAGTTAAGGGATATAAATTATTAGATATACTACTTACAGCTTCCACGAAGATAAagag                                                         <  6:190547/138‑1 (MQ=255)

                                                                                         tttAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAagag                                                         <  3:7569/138‑1 (MQ=255)

                                                                                         tttAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAagag                                                         <  2:53408/138‑1 (MQ=255)

                                                                                         tttAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAagag                                                         <  3:23271/138‑1 (MQ=255)

                                                                                         tttAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAagag                                                         <  3:94506/138‑1 (MQ=255)

                                                                                         tttAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAagag                                                         <  7:258861/138‑1 (MQ=255)

                                                                                         tttAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAagag                                                         <  3:12464/138‑1 (MQ=255)

                                                                                          ttAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTTAATTTTGAAAGTTTCACGGTACGAAATGTAATTGTTATAAATTATTATATATAATAATGACAGCGTACAACAACATAAAGAGGt                                                       >  6:79183/1‑139 (MQ=255)

                                                                                            aaTAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTTGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTACAAGAAGCTAAAGAGGTcc                                                     >  2:8373/1‑139 (MQ=255)

                                                                                                   aTCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCGTTTTTAAATTTTGAAATTTTCACGTTAATAAAGGGAATGGAGATAAATTATTATATATAATAATGACAGCTTCCAAAAAGCTAAAGAGGTCCCTAgcgc                                              >  7:14133/1‑139 (MQ=255)

                                                                                                          attTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGATATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACgg                                        <  2:246918/138‑1 (MQ=255)

                                                                                                            ttAACGGGAGGAAATAATTCTTTGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACgggg                                      <  4:29553/138‑1 (MQ=255)

                                                                                                                   gaggaAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgg                              <  6:120644‑M1/139‑3 (MQ=255)

                                                                                                                    aggaAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAACTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGggg                             <  6:100962‑M1/139‑4 (MQ=255)

                                                                                                                      gaAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggta                           <  1:206348‑M1/139‑6 (MQ=255)

                                                                                                                        aataatTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCGAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtaca                         <  1:63791‑M1/139‑8 (MQ=255)

                                                                                                                            atTCTATGATTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattga                     <  7:177252‑M1/139‑12 (MQ=255)

                                                                                                                            atTATATGAGTCGCTTTTTTAAATTTGGAAAGTTACACTTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattga                     <  3:28155‑M1/139‑12 (MQ=255)

                                                                                                                            atTATATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGATTGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGTGAATTTGgggtacattga                     <  5:262619‑M1/139‑12 (MQ=255)

                                                                                                                                  tGAGTCTCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg               <  7:124219‑M1/139‑18 (MQ=255)

                                                                                                                                  tGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg               <  5:242126‑M1/139‑18 (MQ=255)

                                                                                                                                   tagTCGTTTTTTTAAATTTGGAAAGTTAAACGTTACTAAAGGTAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg               <  3:107093‑M1/137‑18 (MQ=255)

                                                                                                                                   tagTCGCTTTTTTAAATTTTGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg               <  3:36783‑M1/137‑18 (MQ=255)

                                                                                                                                   tagTCGCTTTTTTAAATTTGGAAATTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg               <  7:299968‑M1/137‑18 (MQ=255)

                                                                                                                                   gAGTCTCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGATATAAATTATTAGATATACTACTGACAGCTTCCAAGAATCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg               <  5:172941‑M1/138‑18 (MQ=255)

                                                                                                                                    aGTCTCTTTTTTAAATTTGGAAAGTTACACTTTAAAAAAGGGAATGCAGATAAATTATTAGATATACTACTGACAGCTTCAAAGAAGATAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaa             <  3:168332‑M1/139‑20 (MQ=255)

                                                                                                                                    aGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGTAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaa             <  1:249683‑M1/139‑20 (MQ=255)

                                                                                                                                    aGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagga              <  2:198776‑M1/138‑19 (MQ=255)

                                                                                                                                      tCGCTTTTTTAAATTTGGAAACTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGAAATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGcggtacattgaaaaaggaag            <  8:59008‑M1/138‑21 (MQ=255)

                                                                                                                                          ttttttAAATTTTGAAAGTTACACGTTACTAAAGGTAATGTAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtat       <  5:56174‑M1/139‑26 (MQ=255)

                                                                                                                                          ttttttAAATTTTGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtat       <  6:260438‑M1/139‑26 (MQ=255)

TGTTTACTAAAAATCAGTTTCATCAAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGTATCGGGGTACATTGAAAAAGGAAGAGTAT  >  pSIJ663_pHT315_araE_xy/3591‑3872

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`