BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	pSIJ663_pHT315_araE_xy	3,741	G→T	10.1%	intergenic (+26/‑130)	eryR → / → ampR	–/–

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	pSIJ663_pHT315_araE_xy	3,741	0	G	T	10.1%	19767.7 / inf	6694	intergenic (+26/‑130)	eryR/ampR	–/–
Reads supporting (aligned to +/- strand): ref base G (213/5618); new base T (691/118); total (944/5750)
Fisher's exact test for biased strand distribution p-value = 0.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 6868 total aligned reads displayed.

AATCAGTTTCATCAAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGTATCGGGGTACATTGAAAAAGGAAGAGTATGAGTATTCAA  >  pSIJ663_pHT315_araE_xy/3602‑3882

 aTCAGTTTCATCAAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTg                                                                                                                                               <  5:191351/139‑1 (MQ=255)

  tCAGTTTCATCAAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTgg                                                                                                                                              <  8:182227/139‑1 (MQ=255)

   cattttCAACAACAAATCAAAAAAGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGa                                                                                                                                             <  1:159870/136‑1 (MQ=255)

    aGTTTAATCAAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGa                                                                                                                                             <  1:81425/138‑1 (MQ=255)

           tcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTaca                                                                                                                                     <  7:2754/139‑1 (MQ=255)

           tcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTaca                                                                                                                                     <  4:159013/139‑1 (MQ=255)

           tcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTaca                                                                                                                                     <  3:145532/139‑1 (MQ=255)

           tcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTaca                                                                                                                                     <  6:162207/139‑1 (MQ=255)

           tcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTaca                                                                                                                                     <  3:166568/139‑1 (MQ=255)

           tcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTaca                                                                                                                                     <  5:126262/139‑1 (MQ=255)

           tcaACCAATGAAACAATCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGTGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTaca                                                                                                                                     <  2:309431/139‑1 (MQ=255)

             aaGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACg                                                                                                                                   <  4:292588/139‑1 (MQ=255)

             aaGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACg                                                                                                                                   <  7:175853/139‑1 (MQ=255)

             aaGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACg                                                                                                                                   <  8:19931/139‑1 (MQ=255)

             aaGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACg                                                                                                                                   <  4:266446/139‑1 (MQ=255)

             aaGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACg                                                                                                                                   <  3:314438/139‑1 (MQ=255)

             aaGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACg                                                                                                                                   <  5:98316/139‑1 (MQ=255)

             aaGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACg                                                                                                                                   <  3:4809/139‑1 (MQ=255)

             aaGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACg                                                                                                                                   <  3:68231/139‑1 (MQ=255)

             aaGCAATGAAACACGACAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACg                                                                                                                                   <  4:63186/139‑1 (MQ=255)

                cAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑GTGTTTTTATTTGGGATGTTTCACggt                                                                                                                                 >  3:88304/1‑136 (MQ=255)

                  aTGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACt                                                                                                                              <  7:29758/139‑1 (MQ=255)

                     aaaCACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTaaa                                                                                                                           <  7:156412/139‑1 (MQ=255)

                     aaaCACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTAAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTaaa                                                                                                                           <  1:142486/139‑1 (MQ=255)

                          cGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGaa                                                                                                                      <  2:274772/139‑1 (MQ=255)

                          cGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGaa                                                                                                                      <  5:279695/139‑1 (MQ=255)

                          cGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGaa                                                                                                                      <  8:77673/139‑1 (MQ=255)

                            ccAAAGGAAACAATTAAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATg                                                                                                                    <  8:243230/139‑1 (MQ=255)

                                aGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGgaga                                                                                                                <  3:206426/139‑1 (MQ=255)

                                aGTAAACAATTTAAGTACAATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGATGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGgaga                                                                                                                <  6:310685/139‑1 (MQ=255)

                                  tAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATa                                                                                                              <  8:58817/139‑1 (MQ=255)

                                  agacagaTTTAAGTAACATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATa                                                                                                              <  1:182572/133‑1 (MQ=255)

                                   aaaCAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATa                                                                                                              <  7:141196/138‑1 (MQ=255)

                                    aaCAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATaaa                                                                                                            <  4:210170/139‑1 (MQ=255)

                                      cAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAatt                                                                                                          <  8:35111/139‑1 (MQ=255)

                                         tttAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAattatt                                                                                                       <  3:227602/139‑1 (MQ=255)

                                         tttAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAattatt                                                                                                       <  1:205214/139‑1 (MQ=255)

                                         cagaaGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAattatt                                                                                                       <  5:151071/136‑1 (MQ=255)

                                         cagaaGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAattatt                                                                                                       <  7:75406/136‑1 (MQ=255)

                                           tAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTTGAAATTTAAACGTTAATAAATGGAATGGAAAAAAattat                                                                                                        >  2:164714/1‑136 (MQ=255)

                                            aaGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAg                                                                                                     <  7:96282/138‑1 (MQ=255)

                                            aaGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAg                                                                                                     <  5:111063/138‑1 (MQ=255)

                                             aGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGat                                                                                                   <  2:193005/139‑1 (MQ=255)

                                              gTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGata                                                                                                  <  7:104419/139‑1 (MQ=255)

                                               tACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGatat                                                                                                 <  5:216181/139‑1 (MQ=255)

                                                 ccATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCGTTTTTTTAATTT‑GTAAGTTTAAAGTTTAGAAAAGGTATTTATATAAATTATtatatata                                                                                                >  2:245186/1‑138 (MQ=255)

                                                        ttATGAGCAAGTTTTTTCTATTTTTAATAGTTATCTATTATTTAACTGGAGGAAATAATTCTATTAGTAGC‑TTTTTTAAATTTGGAAAGTTACTCGTTACTAAAGGGAATGGAGATAAAGTATTAGATATACTACTGAc                                                                                        <  3:254606/139‑1 (MQ=255)

                                                        ttATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGAc                                                                                        <  8:268215/139‑1 (MQ=255)

                                                            gAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTTAATTTTGTAAGTTAAACGTTTATAAATGGAATGGTGATAAATTATTTTATATACTACATACAGCt                                                                                    >  6:79254/1‑139 (MQ=255)

                                                             aGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑GGTTTTTTTTTTTGATTGTTAGACGGTACTAAAGGGAATGGGGATTAATTTTTTTATTTAATAATGACAGCtt                                                                                   >  1:100323/1‑139 (MQ=255)

                                                                aaGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCa                                                                                <  2:221649/139‑1 (MQ=255)

                                                                aaGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCa                                                                                <  3:124152/139‑1 (MQ=255)

                                                                 aGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCaa                                                                               <  7:219344/139‑1 (MQ=255)

                                                                 aGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCaa                                                                               <  8:114574/139‑1 (MQ=255)

                                                                 aGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCaa                                                                               <  2:134185/139‑1 (MQ=255)

                                                                  gTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCaag                                                                              <  6:116745/139‑1 (MQ=255)

                                                                  gTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑GTTTTTTAATTTTGTAATTTTAACGTTTATAAATGGTATGGTGATTAATTTTTTTATTTAATAATGTCAGCTTcc                                                                                 >  4:138913/1‑136 (MQ=255)

                                                                   tATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCaaga                                                                             <  6:141688/139‑1 (MQ=255)

                                                                    aTTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCaaga                                                                             <  5:238059/138‑1 (MQ=255)

                                                                              tttAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAagag                                                                   <  4:183045/138‑1 (MQ=255)

                                                                              tttAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAagag                                                                   <  2:53408/138‑1 (MQ=255)

                                                                              tttAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAagag                                                                   <  8:138523/138‑1 (MQ=255)

                                                                              tttAATAGTTATCTATAATTTAACGTGAGGAAATAATTATATTAGTCGC‑TTTTTTAAATTTGGAAATTTACACTTAAATAAAGCGAATGGAGATAAATAATTATATATACTACTTACAGCTTCCAAGAAGATAAagag                                                                   <  6:186434/138‑1 (MQ=255)

                                                                              tttAATAGTTATCTACTAATTAACGCGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACCCGTTACTAAAGGGAAGGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAagag                                                                   <  3:185694/138‑1 (MQ=255)

                                                                                   tAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCt                                                             <  3:29669/139‑1 (MQ=255)

                                                                                     gtaaTCTATATTTTAAGGGGAGGAAATAATTCTATGAGTCGC‑TTTTTAAAATTAGGAAAGTTACACGTAAGTAAAGCGAATGGAGATAAATTATTTGATATACTACTGACACCATCCAAGAAGCTAAAGAGGTCCCTAg                                                           <  5:32197/136‑1 (MQ=255)

                                                                                         tCTATTAGTTAACGGGTTCCAATAATTCTATGAGTCGC‑TTTTTTAAATTTGTAAAGGTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACCGCTTCCAAGACGCTTAACAGGGCCCTAg                                                           <  4:35164/135‑1 (MQ=255)

                                                                                               attTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACgg                                                  <  5:53745/138‑1 (MQ=255)

                                                                                                tttAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACggg                                                 <  5:75410/138‑1 (MQ=255)

                                                                                                tttAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACggg                                                 <  7:197546/138‑1 (MQ=255)

                                                                                                   aaCGGGAGGAAATAATTCTATGAGTCGC‑GTTTTTTAATTTTGTAAGTTTCCCGGTAAGAAAGGGTATGGTGATAAATTATTTGGTATTCTTCTGACCGCTTCCAACCAGCGAAAGCGGTGCCTAGCGCCGCCGGGGGAt                                             >  8:162390/1‑139 (MQ=255)

                                                                                                   aaCGGGAGGAAATAATTCTATGAGTCGC‑GTTTTTAAATTTTGTAATTTAAACGGTTCTAAAGTGAAAGGTGATAAAATATTATATATAAAAATGAAAACTTGCAAGAAACTAAAAATGTGCCTAGCGCCAACGGGGAAt                                             >  4:239507/1‑139 (MQ=255)

                                                                                                     cGGGAGGAAATAATTCTATGAGTCGC‑GGTTTTTTATTTTGTAAGTTTAAAGGGACTAAAGGGAATGGGGATAAATTATTTTTTTTAAATCTGACAGCGTCCAACAAGCAAAATAGGTGCCTAGCGCCGACGGGGGAttt                                           >  4:9044/1‑139 (MQ=255)

                                                                                                      gggAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTG                                          <  7:56748‑M1/139‑1 (MQ=255)

                                                                                                       ggaggaAATAATTCTATGAGTCGC‑TTTTTTAAAATTTGAAAGTTTAACCTTACTAAACGGAATTGATATAAATTAATATATTTTATAATCACCACTTCCAAGAAGCTAAATAGCTCCCGACCGCCGACGGGGTAttttg                                         >  6:289717/1‑137 (MQ=255)

                                                                                                        gaggaAATAATTCTATGAGTCGC‑TTTTTTAAAATTGGAAAGTTACACGTTACTAAAGGGAAAGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgg                                        <  7:256473‑M1/139‑3 (MQ=255)

                                                                                                         aggaAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGggg                                       <  6:53690‑M1/139‑4 (MQ=255)

                                                                                                         aggaAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGggg                                       <  5:257722‑M1/139‑4 (MQ=255)

                                                                                                             aataatTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtaca                                   <  3:275177‑M1/139‑8 (MQ=255)

                                                                                                             aataatTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtaca                                   <  2:82332‑M1/139‑8 (MQ=255)

                                                                                                             aataatTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtaca                                   <  7:123856‑M1/139‑8 (MQ=255)

                                                                                                                 atTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattga                               <  6:215344‑M1/139‑12 (MQ=255)

                                                                                                                   tCTATGAGTCGC‑TTTTTTAAATTTGGAAATTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTTACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaa                              <  1:58149‑M1/138‑13 (MQ=255)

                                                                                                                    cTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaa                             <  1:29457‑M1/138‑14 (MQ=255)

                                                                                                                       tGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                         <  5:170453‑M1/139‑18 (MQ=255)

                                                                                                                       agAGTCGC‑TTTTATAAATTTGGAAAGTTAGCCGCTAATAATGGGAATGGAGATTAATTATTATATATACTAGTGCACGCATCAAAGACGCTAAAGTGGTCCCTAGCGCCTACGGGGAATTT‑‑‑‑‑GGGGTACATTGAAAAAgg                    <  2:102851/138‑1 (MQ=255)

                                                                                                                         aGTCGC‑TTTTTTAAATTTGGTAAGTTACTCGTTACTAAAGTGTATTTATATAAATTATTATATATACTAATGACAGCTTCCATGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaa                       <  5:299178‑M1/139‑20 (MQ=255)

                                                                                                                         aGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAATAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaa                       <  3:106713‑M1/139‑20 (MQ=255)

                                                                                                                         aGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaa                       <  7:241561‑M1/139‑20 (MQ=255)

                                                                                                                         aGTCGA‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGTAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagga                        <  8:221497‑M1/138‑19 (MQ=255)

                                                                                                                          gTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGATATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaa                       <  1:227050‑M1/138‑20 (MQ=255)

                                                                                                                          gTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaa                       <  1:35406‑M1/138‑20 (MQ=255)

                                                                                                                          gTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaa                       <  8:296099‑M1/138‑20 (MQ=255)

                                                                                                                           gtctattttttAAATTTTGAAAGTTACACGTTACTAAAGTTAATGGAGATAAATTATTATATATACTACTGACAGCTTCCAAGAAGATAAAGAGGTCCCTAGCGCCTACGGGTAATTT‑‑‑‑‑GGGGTACATTGAAAAAGGaa                  <  4:111651/133‑1 (MQ=255)

                                                                                                                             gC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagt                   <  6:237578‑M1/139‑24 (MQ=255)

                                                                                                                                ttttttAAATTTTGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtat                 <  6:260438‑M1/139‑26 (MQ=255)

                                                                                                                                 tttttAAATTTGGAAAGTTACACTTTACTAAAGGTAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtat                 <  1:119738‑M1/138‑26 (MQ=255)

                                                                                                                                  ttttAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtat                 <  1:247183‑M1/137‑26 (MQ=255)

                                                                                                                                  ttttAAATTTGGAAAGATACACGTTACTAAAGTGAATGGATATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACTGGGAATTTGggttacattgaaaaaggaagagtat                 <  7:35570‑M1/137‑26 (MQ=255)

                                                                                                                                           tGGAAAGTTACACGTTACTAAAGGTAATGGAGATAAATTATTAGATATACTACTGACAGCATCCAAGAAGCTAATGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaa       <  1:270663‑M1/138‑36 (MQ=255)

AATCAGTTTCATCAAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGTATCGGGGTACATTGAAAAAGGAAGAGTATGAGTATTCAA  >  pSIJ663_pHT315_araE_xy/3602‑3882

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`