BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	pSIJ663_pHT315_araE_xy	3,749	A→T	5.8%	intergenic (+34/‑122)	eryR → / → ampR	–/–

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	pSIJ663_pHT315_araE_xy	3,749	0	A	T	5.8%	18701.3 / 182.0	5893	intergenic (+34/‑122)	eryR/ampR	–/–
Reads supporting (aligned to +/- strand): ref base A (517/4934); new base T (407/11); total (934/4959)
Fisher's exact test for biased strand distribution p-value = 0.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 6582 total aligned reads displayed.

CATCAAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGTATCGGGGTACATTGAAAAAGGAAGAGTATGAGTATTCAACATTT  >  pSIJ663_pHT315_araE_xy/3611‑3887

  tcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTaca                                                                                                                                          <  8:57889/139‑1 (MQ=255)

  tcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTaca                                                                                                                                          <  5:152454/139‑1 (MQ=255)

  tcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTaca                                                                                                                                          <  6:240402/139‑1 (MQ=255)

  tcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTaca                                                                                                                                          <  7:157369/139‑1 (MQ=255)

  tcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTaca                                                                                                                                          <  1:263733/139‑1 (MQ=255)

  tcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTaca                                                                                                                                          <  7:256828/139‑1 (MQ=255)

  tcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTaca                                                                                                                                          <  7:2754/139‑1 (MQ=255)

  tcaAGCAATGAAACACGCCAAACTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTaca                                                                                                                                          <  3:65035/139‑1 (MQ=255)

  tcaAGCAATCAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTaca                                                                                                                                          <  7:220166/139‑1 (MQ=255)

    aatcAATGAAACAAGCCAAAGTAAACAATTTAATTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACg                                                                                                                                        <  2:271714/136‑1 (MQ=255)

    aaGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACg                                                                                                                                        <  3:31188/139‑1 (MQ=255)

    aaGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACg                                                                                                                                        <  5:7842/139‑1 (MQ=255)

    aaGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACg                                                                                                                                        <  6:191759/139‑1 (MQ=255)

    aaGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACg                                                                                                                                        <  7:58147/139‑1 (MQ=255)

       cAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTa                                                                                                                                     <  5:172883/139‑1 (MQ=255)

        aaTGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTAc                                                                                                                                    <  4:136528/139‑1 (MQ=255)

         aTGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACt                                                                                                                                   <  4:61621/139‑1 (MQ=255)

            aaaCACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTaaa                                                                                                                                <  8:34339/139‑1 (MQ=255)

             aaCACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTaaa                                                                                                                                <  6:59144/138‑1 (MQ=255)

             aaCACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTaaa                                                                                                                                <  1:179245/138‑1 (MQ=255)

                acGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGa                                                                                                                            <  2:30659/139‑1 (MQ=255)

                 cGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGaa                                                                                                                           <  8:77673/139‑1 (MQ=255)

                 cGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGaa                                                                                                                           <  4:1099/139‑1 (MQ=255)

                 cGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGaa                                                                                                                           <  1:77358/139‑1 (MQ=255)

                 cGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCGTTTTTTAATTTTGAAATTTACACGTTAATAAAGGGaa                                                                                                                           >  4:266070/1‑139 (MQ=255)

                 cGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATAATTTAACGGGAGGAAATAATTCTATGAGTCGCGTTTTTAAATTTTGAAATTTACACCTTAATAAAGTGa                                                                                                                            >  2:189874/1‑138 (MQ=255)

                   ccAAAGTAAAGAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATg                                                                                                                         <  8:115976/139‑1 (MQ=255)

                   ccAAAGTAAACAATTTCAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATg                                                                                                                         <  7:241874/139‑1 (MQ=255)

                         tAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATa                                                                                                                   <  6:1444/139‑1 (MQ=255)

                         tAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATa                                                                                                                   <  4:157504/139‑1 (MQ=255)

                          aaaCAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATa                                                                                                                   <  1:31720/138‑1 (MQ=255)

                             cAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGCTAAatt                                                                                                               <  8:269288/139‑1 (MQ=255)

                               aTTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAattat                                                                                                             <  6:217481/139‑1 (MQ=255)

                                tttCAGTACCATTAGTTATGAGCAAGTTTTGTCTATATTTAATAGTTATCTATTATTTAACGTCATGAACTATTTCTATGAGTCGCTTTTTTAAATTAGGAAAGTTACACGGTACTAAAGGGAATTGAGATAAattatt                                                                                                            <  2:215802/139‑1 (MQ=255)

                                tttAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAattatt                                                                                                            <  5:34229/139‑1 (MQ=255)

                                tttAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAattatt                                                                                                            <  6:293534/139‑1 (MQ=255)

                                tttAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAattatt                                                                                                            <  2:50384/139‑1 (MQ=255)

                                tttAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAattatt                                                                                                            <  7:298330/139‑1 (MQ=255)

                                tttAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAattatt                                                                                                            <  8:295620/139‑1 (MQ=255)

                                tttAAGTACCATTACTTATGAGCAAGTATTGTCTAATTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAattatt                                                                                                            <  6:26424/139‑1 (MQ=255)

                                attAAGTAACATTACTTATGATCAAGTATTGTCTATTATTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAattatt                                                                                                            <  1:140404/138‑1 (MQ=255)

                                   aaGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAg                                                                                                          <  6:166745/138‑1 (MQ=255)

                                   aaGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAg                                                                                                          <  2:159512/138‑1 (MQ=255)

                                    aGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGat                                                                                                        <  1:118846/139‑1 (MQ=255)

                                    aGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGat                                                                                                        <  5:111448/139‑1 (MQ=255)

                                    aGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGat                                                                                                        <  7:107210/139‑1 (MQ=255)

                                     gTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGata                                                                                                       <  6:82664/139‑1 (MQ=255)

                                     acagcATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGata                                                                                                       <  7:199691/135‑1 (MQ=255)

                                              cTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGa                                                                                              <  8:138911/139‑1 (MQ=255)

                                              cTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGa                                                                                              <  4:237199/139‑1 (MQ=255)

                                              cTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGa                                                                                              <  2:129982/139‑1 (MQ=255)

                                                 aTGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGAc                                                                                             <  7:125111/137‑1 (MQ=255)

                                                   gAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCGTTTTTTAATTTTGTAATTTTAACCTTACTAAAGGGAATTGAGATAAATTATTATATTTAATACTTACAGCt                                                                                         >  5:261248/1‑139 (MQ=255)

                                                    aGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCGGTTTTTTTTTTTGATTGTTAGACGGTACTAAAGGGAATGGGGATTAATTTTTTTATTTAATAATGACAGCtt                                                                                        >  1:100323/1‑139 (MQ=255)

                                                         gTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCaag                                                                                   <  8:221298/139‑1 (MQ=255)

                                                         gTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCaag                                                                                   <  5:99611/139‑1 (MQ=255)

                                                            ttGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCaagaag                                                                                <  7:7662/139‑1 (MQ=255)

                                                               tCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTa                                                                             <  5:300586/139‑1 (MQ=255)

                                                                 tATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCGTTTTTTTATTTTGAAAGTTTCACGTTAGTTAAGGGTATTGTGTTTAATTTTTTTTTTTAGTACTGACAGCATCCAAGAAGCTaaa                                                                           >  3:122955/1‑139 (MQ=255)

                                                                 tATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCGTTTTTTAATTTTGTAATTTGGACGGTTCTATAGGGATTGGTGATTAATTATTTGTGATAAAAATGTCACCTTCCCAGAAACTaaa                                                                           >  4:292427/1‑139 (MQ=255)

                                                                 tATTTTTAATAGTTATCTATTATTTAAAGGGAGGAAATAATTCTATTAGTATCTTTTTTAAATTTGGAAAGTTACACTTTTCTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTaaa                                                                           <  3:271071/139‑1 (MQ=255)

                                                                 tATTTTTAATAGTTATATATTATTTAACGGGATTAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGCCAGCTTCCAAGAAGCTaaa                                                                           <  7:142046/139‑1 (MQ=255)

                                                                   tttttAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAaga                                                                         <  3:222481/139‑1 (MQ=255)

                                                                   tttttAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAag                                                                          <  5:241052/138‑1 (MQ=255)

                                                                     tttAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAagag                                                                        <  2:100922/138‑1 (MQ=255)

                                                                     tttAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAagag                                                                        <  1:29133/138‑1 (MQ=255)

                                                                     tttAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAagag                                                                        <  5:55880/138‑1 (MQ=255)

                                                                         aTAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCGTTTTTTTATTTTGATATTTTAACGTTTATAAAGGGAATGGAGTTAAATTATTATATATAATAATGACAGCATCCAAGAAGCTTAAGAGGTccc                                                                   >  4:34220/1‑139 (MQ=255)

                                                                               aTCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCGTTTTTTAATTTTGAAAGTTTAACGTTAATAAATGGAATTGAGATAAATTATTATATATACTAATGACAGCTTCCAAAAAGATAAAAACGTACCTAgcgc                                                             >  2:8023/1‑139 (MQ=255)

                                                                                      attTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACgg                                                       <  7:36252/138‑1 (MQ=255)

                                                                                       tttAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTTGAAATTTAAAAGTTACTAAATGGAATGGAGATAAATTATTATATATAATACTGACAGCTTACAAGAAACTAAAGATGTCCCTAGCGCCGACgggg                                                     >  6:119653/1‑139 (MQ=255)

                                                                                       tttAACGGGAGGAAATAATTCTATGAGTCGCGTTTTTAAATTTTGAAAATTACAACTTAATAAAGGGTATGGAGATAAAATATTATATATAATACTGACACCTTACAAAAAGCTAAAGAGGTACCTAGCGCCTACgggg                                                     >  1:53855/1‑139 (MQ=255)

                                                                                          aaCGGGAGGAAATAATTCTATGAGTCGCGTTTTTTAATTTTGTAATTTACACCTTAATAAAAGGAATGGAGATAAATTATTATATTTAATACTGACAGCATCCAATAAGCAAAAGAGGTCCCAAGCGCCGACCGGGAAt                                                  >  3:86402/1‑139 (MQ=255)

                                                                                          aaCGGGAGGAAATAATTCTATGAGTCGCGTTTTTTAATTTTGTAAGTTTCCCGGTAAGAAAGGGTATGGTGATAAATTATTTGGTATTCTTCTGACCGCTTCCAACCAGCGAAAGCGGTGCCTAGCGCCGCCGGGGGAt                                                  >  8:162390/1‑139 (MQ=255)

                                                                                               gaggaAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgg                                             <  2:240650‑M1/139‑3 (MQ=255)

                                                                                                    aataatTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtac                                         <  1:245186‑M1/138‑7 (MQ=255)

                                                                                                      taatTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacatt                                      <  3:292003‑M1/139‑10 (MQ=255)

                                                                                                        atTCTATGATTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattga                                    <  7:177252‑M1/139‑12 (MQ=255)

                                                                                                           cTATGATTCTCTTTTTTAAATTTGTAAAGTTACACGTTACTAAAGGTAATGGATATAAATTATTATATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGggttacattgaaa                                  <  4:182171‑M1/138‑14 (MQ=255)

                                                                                                              tcagtCGCTGATTTAAATTTGCAAAGTTACCCGTTACTAAAGGGAATGGATCTAAATTATTAGAAATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                              <  8:18213‑M1/137‑18 (MQ=255)

                                                                                                              tGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATTGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                              <  3:202944‑M1/139‑18 (MQ=255)

                                                                                                              tGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                              <  3:104319‑M1/139‑18 (MQ=255)

                                                                                                              tGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                              <  4:90567‑M1/139‑18 (MQ=255)

                                                                                                              tGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                              <  5:127926‑M1/139‑18 (MQ=255)

                                                                                                              tGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCAAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                              <  5:219726‑M1/139‑18 (MQ=255)

                                                                                                               tagTCTCTTTTTTAAATTTGGAAAGTTACAAGTTACTATAGTGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                              <  3:25188‑M1/137‑18 (MQ=255)

                                                                                                               tagTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGATATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                              <  3:45175‑M1/137‑18 (MQ=255)

                                                                                                               tagTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTTACAGCTTCCAAGAAGATAAAGAGGTCCCTAGCGCCTACGGTGAATTTGgggtacattgaaaaagg                              <  5:281667‑M1/137‑18 (MQ=255)

                                                                                                               gAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGTAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                              <  3:184234‑M1/138‑18 (MQ=255)

                                                                                                               gAGTCCCTTTTTTAAATTTGGAAAGTTACACGTTACTAATGGTAATGTAGATAAATTATTAGATATACTACTGACAGCTTCCAACAAGCTAACGAGGTCCCTAGCGCCTACGGTGAATTTGgggtacattgaaaaagg                              <  1:222187‑M1/138‑18 (MQ=255)

                                                                                                               gAGGCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                              <  3:163757‑M1/138‑18 (MQ=255)

                                                                                                                aGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaa                            <  7:97732‑M1/139‑20 (MQ=255)

                                                                                                                aGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaa                            <  8:198733‑M1/139‑20 (MQ=255)

                                                                                                                aGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagga                             <  3:143586‑M1/138‑19 (MQ=255)

                                                                                                                aGTCGCTTTTTTAAATTTGGAAAGTTACACGTTAATAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaa                            <  1:157614‑M1/139‑20 (MQ=255)

                                                                                                                  tCGATTTTTTAAATTTGGAAAGTTAAACTTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGATGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaag                           <  6:31390‑M1/138‑21 (MQ=255)

                                                                                                                      ttttttAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtat                      <  1:202715‑M1/139‑26 (MQ=255)

                                                                                                                        ttttAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtat                      <  8:24217‑M1/137‑26 (MQ=255)

                                                                                                                             aaTTTGGAAAGTTACACGTTACTAAAGGGAATGGATATAAATTATAAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtatt               <  5:195677‑M1/139‑33 (MQ=255)

                                                                                                                                        ttACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagga                             <  4:208832‑M1/114‑19 (MQ=255)

CATCAAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGTATCGGGGTACATTGAAAAAGGAAGAGTATGAGTATTCAACATTT  >  pSIJ663_pHT315_araE_xy/3611‑3887

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`