BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	pSIJ663_pHT315_araE_xy	4,625	A→C	7.0%	K252T (AAG→ACG)	ampR →

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	pSIJ663_pHT315_araE_xy	4,625	0	A	C	7.0%	15296.3 / 191.6	5764	K252T (AAG→ACG)	ampR
Reads supporting (aligned to +/- strand): ref base A (3170/2032); new base C (52/476); total (3230/2534)
Fisher's exact test for biased strand distribution p-value = 0.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 5856 total aligned reads displayed.

TAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGT‑A‑AGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTA  >  pSIJ663_pHT315_araE_xy/4487‑4763

  gACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGT‑A‑AGc                                                                                                                                          >  5:5028/1‑139 (MQ=255)

   ccTGGATGGAGGCGGATAAAGTTGCCGGACCACTTCTGCGCTCGGCCCTTCCGGCGGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGT‑A‑AGcc                                                                                                                                         <  2:311871/138‑1 (MQ=255)

   aCTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGTGGCCAGATGGT‑A‑AGcc                                                                                                                                         >  5:173704/1‑139 (MQ=255)

   aCTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGT‑A‑AGcc                                                                                                                                         >  3:275788/1‑139 (MQ=255)

    cTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGT‑A‑AGccc                                                                                                                                        >  7:128382/1‑139 (MQ=255)

    cTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGT‑A‑AGccc                                                                                                                                        >  7:188738/1‑139 (MQ=255)

     tggatggaGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGT‑A‑AGCCCt                                                                                                                                       >  4:2779/1‑139 (MQ=255)

      ggatggaGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGT‑A‑AGCCCTc                                                                                                                                      <  4:253079/139‑1 (MQ=255)

      ggatggaGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGT‑A‑AGCCCTc                                                                                                                                      >  1:211196/1‑139 (MQ=255)

      ggatggaGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGT‑A‑AGCCCTc                                                                                                                                      >  3:155769/1‑139 (MQ=255)

      ggatggaGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCGGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGT‑A‑AGCCCTc                                                                                                                                      <  6:41482/139‑1 (MQ=255)

       gatggaGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGT‑A‑Ag                                                                                                                                           >  8:81605/1‑133 (MQ=255)

       gatggaGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGT‑A‑AGCCCTc                                                                                                                                      <  6:2943/138‑1 (MQ=255)

          ggaGTCGGATAAAGGTGCCGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGT‑A‑AGCCCTCCCg                                                                                                                                   <  2:281907/138‑1 (MQ=255)

           gaGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGT‑A‑AGCCCTCCCGTa                                                                                                                                 <  7:7335/139‑1 (MQ=255)

           gaGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGT‑A‑AGCCCTCCCGTa                                                                                                                                 <  5:249729/139‑1 (MQ=255)

           gaGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGT‑A‑AGCCCTCCCGTa                                                                                                                                 <  3:114598/139‑1 (MQ=255)

            aGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGT‑A‑AGCCCTCCCGTAt                                                                                                                                >  8:205276/1‑139 (MQ=255)

             ggCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGT‑A‑AGCCCTCCCGTATc                                                                                                                               >  3:136591/1‑139 (MQ=255)

              gCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTTGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGT‑A‑AGCCCTCCCGTATCg                                                                                                                              <  8:55573/139‑1 (MQ=255)

              gCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGT‑A‑AGCCCTCCCGTATCg                                                                                                                              <  2:25629/139‑1 (MQ=255)

              gCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGT‑A‑AGCCCTCCCGTATCg                                                                                                                              <  8:225129/139‑1 (MQ=255)

                ggATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGT‑A‑AGCCCTCCCGTATCGTa                                                                                                                            <  7:38746/139‑1 (MQ=255)

                ggATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGT‑A‑AGCCCTCCCGTATCGTa                                                                                                                            <  4:136591/139‑1 (MQ=255)

                 gATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCTGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGT‑A‑AGCCCTCCCGTATCGTAg                                                                                                                           >  7:30233/1‑139 (MQ=255)

                 gATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGT‑A‑Ag                                                                                                                                           <  3:231770/123‑1 (MQ=255)

                 gATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGT‑A‑AGCCCTc                                                                                                                                      >  3:47635/1‑128 (MQ=255)

                 gATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGT‑A‑AGCCCTCCCGTATCGTAg                                                                                                                           <  4:301348/139‑1 (MQ=255)

                 gATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGT‑A‑AGCCCTCCCGTATCGTAg                                                                                                                           >  1:298805/1‑139 (MQ=255)

                 gATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGT‑A‑AGCCCTCCCGTATCGTAg                                                                                                                           >  2:10618/1‑139 (MQ=255)

                 gATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGT‑A‑AGCCCTCCCGTATCGTAg                                                                                                                           >  3:181239/1‑139 (MQ=255)

                     aaGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGT‑A‑AGCCCTCCCGTATCGTAGTTAt                                                                                                                       <  1:234218/139‑1 (MQ=255)

                          ccagacccACTTCTGCGCGCGGCCCTGCGGGGGGGGTGTTTTATGGCTTAAAAATCTGGCGCCGGTGAGCGGGGGGCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGT‑A‑AGCCCTCCCGTATCGTAGTTATCAaca                                                                                                                  <  6:273078/133‑1 (MQ=255)

                           cAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTGTTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGT‑A‑AGCCCTCCCGTATCGTAGTTATCTacac                                                                                                                 >  1:171598/1‑139 (MQ=255)

                           cAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGT‑A‑AGcc                                                                                                                                         <  2:90024/115‑1 (MQ=255)

                           cAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGT‑A‑AGCCCTCCCGTATCGTAGTTATCTacac                                                                                                                 >  7:254949/1‑139 (MQ=255)

                            aGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGT‑A‑AGCCCTCCCGTa                                                                                                                                 >  2:231607/1‑122 (MQ=255)

                                         cgcTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGT‑A‑AGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAgg                                                                                                   >  1:57739/1‑139 (MQ=255)

                                           cTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGT‑A‑AGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCa                                                                                                 >  7:280727/1‑139 (MQ=255)

                                              ggCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGT‑A‑AGc                                                                                                                                          >  8:161470/1‑95 (MQ=255)

                                              ggCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGT‑A‑AGCCCTc                                                                                                                                      >  6:230354/1‑99 (MQ=255)

                                              ggCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGT‑A‑AGCCCTc                                                                                                                                      >  5:73386/1‑99 (MQ=255)

                                              ggCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGT‑A‑AGCCCTCCCGTa                                                                                                                                 <  6:15208/104‑1 (MQ=255)

                                              ggCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGT‑A‑AGCCCTCCCGTATCg                                                                                                                              <  3:25752/107‑1 (MQ=255)

                                              ggCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGT‑A‑AGCCCTCCCGTATCGTa                                                                                                                            <  5:98688/109‑1 (MQ=255)

                                              ggCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGT‑A‑AGCCCTCCCGTATCGTAGTTATCTACACTACGTGGAGTCAGGCAAc                                                                                               >  8:251831/1‑138 (MQ=255)

                                              ggCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGT‑A‑AGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCGGGCAAc                                                                                               >  3:68529/1‑138 (MQ=255)

                                              ggCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGT‑A‑AGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAAc                                                                                               >  6:114455/1‑138 (MQ=255)

                                              ggCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGT‑A‑AGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGGCAGGCAAc                                                                                               >  1:191807/1‑138 (MQ=255)

                                                     cgggcggggtGGTTTTTTGCTTATAAATGGGCAGCGGGGGAGGGGGCGGCTCGGGTTAGAATTGCCGCCCTGGGGCCTTATGGT‑A‑CCCCCTCCCTTATCGTAGTTATCAACACGACGGGGAGTCAGGCAACTATGGAt                                                                                        <  4:227998/136‑1 (MQ=255)

                                                      cGGCTGGCTGTTTTATGGCTCATAAATGTGGCGCCGGTGAGCGGGGGGCGCGCGGTATCATTGCCGCCCTGGGCCCCTTTGTA‑A‑AGCCCTCCCGTATCGTTGTATTCACCACGACGGGGAGTCAGGCAACTATGGATGa                                                                                      <  4:156179/139‑1 (MQ=255)

                                                          tggctggTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGT‑A‑AGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACg                                                                                   >  1:124159/1‑138 (MQ=255)

                                                            gctggTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGT‑A‑Ag                                                                                                                                           >  6:131618/1‑80 (MQ=255)

                                                                        atGATAAATCTGGCGCGGGTGAGCGTGGGTCGCGCTTTATCTTTGAAGCACTGGGGCCAGTTGGT‑A‑ACCCCTCCCTTATCGTTTTTATATACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGc                                                                    <  8:41904/138‑1 (MQ=255)

                                                                          gATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGT‑A‑AGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACTGATCGCt                                                                   >  7:112350/1‑138 (MQ=255)

                                                                          gATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGT‑A‑AGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCt                                                                   >  1:147653/1‑138 (MQ=255)

                                                                          gATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGT‑A‑AGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTg                                                                  >  2:49324/1‑139 (MQ=255)

                                                                           aTAACTCTGGAGCCGGTGCGCGGGGGTCGGGCGGTATCATTCCCCCCCTGGGGCCAGATGGA‑A‑CGCCCTCCCGTATCGTAGTTAACACCACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCt                                                                   <  8:305729/137‑1 (MQ=255)

                                                                           aTAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGT‑A‑Ag                                                                                                                                           <  2:66413/65‑1 (MQ=255)

                                                                           aTAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGT‑A‑AGCCCTc                                                                                                                                      <  1:100164/70‑1 (MQ=255)

                                                                           aTAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGT‑A‑AGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCt                                                                   >  7:147930/1‑137 (MQ=255)

                                                                                   ggAGCCGGTGCGGGGGGGTCTGGCGGTATCATTCCAGCCCGGGGGCCATATGTA‑A‑CCCCCTCCCGTATCGTAGTTATATCCACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTg                                                         <  3:202858/139‑1 (MQ=255)

                                                                                     aGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGT‑A‑AGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGcc                                                       >  7:52275/1‑139 (MQ=255)

                                                                                     aGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGT‑A‑AGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGcc                                                       >  2:184380/1‑139 (MQ=255)

                                                                                     aGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGT‑A‑AGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGcc                                                       >  1:78050/1‑139 (MQ=255)

                                                                                     aGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGT‑A‑AGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGcc                                                       >  5:156538/1‑139 (MQ=255)

                                                                                        gggggAGCGGGGGTCTCGCGTTATCATTGCCGCACGGGGCCCAGAGGGT‑A‑CGCCCCCCCGTAGCGTAGTTATCACCACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCa                                                    <  6:254269/135‑1 (MQ=255)

                                                                                        gggTGTGGGTGGGGCGGGCGTTATCATTGCCGCAGGGGGGCCAGATGTT‑A‑AGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCa                                                    <  4:95357/138‑1 (MQ=255)

                                                                                        cGGTGTGGGGGGGTCTCGGGTTATAATTGCAGCACTGGGGCCAGA‑GGT‑ACAGCCCTCCCGTACCGTATTTAACTACACGTCGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCa                                                    <  6:172534/139‑1 (MQ=255)

                                                                                          gttcgCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGT‑A‑AGCCATCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACt                                                  >  8:141927/5‑139 (MQ=255)

                                                                                          gTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGT‑A‑AGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACt                                                  >  5:114730/1‑139 (MQ=255)

                                                                                          gTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGT‑A‑AGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACt                                                  >  5:269429/1‑139 (MQ=255)

                                                                                              gCGTGGGTCCCGCGTTATCCTGGCACCAGGGGGGCCAG‑TGGTGA‑CGCCCACCCGTATCTTTGTTATCACCACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGAtt                                              <  1:38564/139‑1 (MQ=255)

                                                                                                 tGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGT‑A‑Ag                                                                                                                                           <  8:183368/43‑1 (MQ=255)

                                                                                                   ggTGTCGCGGTATAATTGCAGCCCGGGGGCCAGTTGGT‑A‑AGCCCTCCCGTATCGTATTTAAATACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCa                                         <  5:224728/139‑1 (MQ=255)

                                                                                                    gTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGT‑A‑AGCCCTCCCGTAt                                                                                                                                <  6:67045/51‑1 (MQ=255)

                                                                                                        cgcgGTACAATGGCAGCCCGGGGGCAAGTTGGT‑A‑CCCCCGCCCTTATCGTATTTATCAACACGACGGGGAGTCAGGGAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGt                                    <  8:118270/139‑1 (MQ=255)

                                                                                                         gcgGTATCATTGCAGCACTGGGGCCAGATGGT‑A‑AGCCCTc                                                                                                                                      >  5:28770/1‑40 (MQ=255)

                                                                                                             tATCATTGCAGCACTGGGGCCAGATGGT‑A‑AGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGATCGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTg                               >  7:230082/1‑139 (MQ=255)

                                                                                                               tCATTGCAGCACTGGGGCCAGATGGT‑A‑AGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGt                              >  6:88361/1‑138 (MQ=255)

                                                                                                                   tGCAGCACTGGGGCCAGATGGT‑A‑AGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGAc                         >  1:278121/1‑139 (MQ=255)

                                                                                                                   tGCAGCACTGGGGCCAGATGGT‑A‑AGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGAGTAAGCATTGGTAACTGTCAGAc                         >  2:164708/1‑139 (MQ=255)

                                                                                                                    gcagcaCGGGGGCCAGATGGT‑A‑CGCCCTCCGGTTTCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGAc                         <  4:200887/138‑1 (MQ=255)

                                                                                                                     cagcaCTGGGGCCAGATGGT‑A‑AGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGAcc                        >  5:135212/1‑138 (MQ=255)

                                                                                                                       gcaCTGGGGCCAGATGGT‑A‑AGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAg                     >  4:68640/1‑139 (MQ=255)

                                                                                                                         ccgggggCCAGTTTGT‑A‑CGCCCTCCTGTATCGTATTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGtt                   <  7:92651/136‑1 (MQ=255)

                                                                                                                           ggggcCCAGATGGA‑A‑CGCCCTCCGGTATCTTAGTTACCACCACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTa                 <  5:199145/138‑1 (MQ=255)

                                                                                                                             gggCCATATTGT‑A‑AGCCCTCCCGTATAGTATTTATCAACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTa                 <  3:90909/137‑1 (MQ=255)

                                                                                                                             gggCCAGATGGT‑A‑AGCCCTCCCGTATCGTAGTTATCTACACGTCGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTa                 <  8:280381/137‑1 (MQ=255)

                                                                                                                             gggCCAGATGGT‑A‑AGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACt               >  3:300234/1‑139 (MQ=255)

                                                                                                                             gggCCAGATGGT‑A‑AGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACt               >  4:39506/1‑139 (MQ=255)

                                                                                                                              ggCCCGCTGGT‑A‑ACCCCCCCGGTAGCGCTCTCATCCCCACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTc              <  6:200286/139‑1 (MQ=255)

                                                                                                                                  aGATGGT‑A‑AGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCactg          >  7:169880/1‑136 (MQ=255)

                                                                                                                                  aGATGGT‑A‑AGCCCGCCCGTATGGTTGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCatat          <  2:207434/139‑1 (MQ=255)

                                                                                                                                    aTGGT‑A‑AGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCatata         >  1:256953/1‑138 (MQ=255)

                                                                                                                                    aTGGT‑A‑AGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGACAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCCCTGATTAAGCATAGGTAACTGTCAGACCAAGTTTACTCatata         >  2:139281/1‑138 (MQ=255)

                                                                                                                                       gT‑A‑AGCCCTCCCGTATCGTAGTTATCTACACGCCGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACt     >  4:112606/1‑139 (MQ=255)

                                                                                                                                       gT‑A‑AGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCatat          <  5:307180/134‑1 (MQ=255)

                                                                                                                                          a‑aGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCt                                                                   >  7:207274/1‑75 (MQ=255)

                                                                                                                                          a‑aGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACtt    >  4:176708/1‑138 (MQ=255)

TAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGT‑A‑AGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTA  >  pSIJ663_pHT315_araE_xy/4487‑4763

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`