BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	pSIJ663_pHT315_araE_xy	5,049	C→T	100%	intergenic (+318/–)	ampR → / –	–/–

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	pSIJ663_pHT315_araE_xy	5,049	0	C	T	100.0%	26750.4 / ‑6.6	7681	intergenic (+318/–)	ampR/–	–/–
Reads supporting (aligned to +/- strand): ref base C (1/1); major base T (2549/5097); minor base G (6/14); total (2568/5113)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 7846 total aligned reads displayed.

TTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAA‑ACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGA  >  pSIJ663_pHT315_araE_xy/4912‑5184

   tttCTGCGCGTAATCTGCTGCTTGCAAAAAAAAAA‑ACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCtt                                                                                                                                      <  5:275105/138‑1 (MQ=255)

    ttCTGCGCGTAATCTGCTGCTTGCAAACAAAAAA‑ACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGttcttc                                                                                                                                     <  7:253893/138‑1 (MQ=255)

       tGCGCGTAATCTGCTGCTTGCAAACAAAAAA‑ACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAgt                                                                                                                                 >  8:163874/1‑139 (MQ=255)

        gcgcGTAATCTGCTGCTTGCAAACAAAAAA‑ACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCtt                                                                                                                                      >  2:72003/1‑133 (MQ=255)

        gcgcGTAATCTGCTGCTTGCAAACAAAAAA‑ACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAgtg                                                                                                                                >  4:5625/1‑139 (MQ=255)

        gcgcGTAAGCTGCTGCTTGCAAACAAAAAA‑ACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAgtg                                                                                                                                <  2:128594/139‑1 (MQ=255)

         cgcgTAATCTGCTGCTTGCAAACAAAAAA‑ACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAgtgt                                                                                                                               >  3:133312/1‑139 (MQ=255)

         cgcgTAATCTGCTGCTTGCAAACAAAAAA‑ACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAgtg                                                                                                                                <  5:253555/138‑1 (MQ=255)

         cgcgTAATCTGCTGCTTGCAAACAAAAAA‑ACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAgtg                                                                                                                                <  2:1553/138‑1 (MQ=255)

         cgcgTAATCTGCTGCTTGCAAACAAAAAA‑ACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAgtg                                                                                                                                <  3:116043/138‑1 (MQ=255)

         cgcgTAATCTGCTGCTTGCAAACAAAAAA‑ACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAg                                                                                                                                  <  7:130050/136‑1 (MQ=255)

            gTAATCTGCTGCTTGCAAACAAAAAA‑ACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGc                                                                                                                            <  2:206252/139‑1 (MQ=255)

                   gctgctTGCAAACAAAAAA‑ACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATAAAGAGCTACCAACTGTTTTTCATAAGCTAACTGGCTTCAGCAGACCGCAGATACCAAAAACTGTTCATCTAGTTTAGCCGTAGt                                                                                                                      >  8:205831/1‑138 (MQ=255)

                    ctgctTGCAAAAAAAAAA‑ACCACCGCTACCAGGGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGt                                                                                                                      <  7:300753/137‑1 (MQ=255)

                      gctTGCAAACAAAAAA‑ACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAgg                                                                                                                  >  4:130469/1‑139 (MQ=255)

                      gctTGCAAACAAAAAA‑ACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAgg                                                                                                                  >  3:66087/1‑139 (MQ=255)

                      gctTGCAAACAAAAAA‑ACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACGCTTTTTACGCAGGTAACAGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTTgg                                                                                                                  >  5:5597/1‑139 (MQ=255)

                      gctTGCAAACAAAAAA‑ACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCGACCAACTCTTTTTACGCAGGTAACTGGCTTCACCAGAGCGCAGCTACCAAAAAATGTTGTTATAGTGTATCCGGAGTTAgg                                                                                                                  >  6:44440/1‑139 (MQ=255)

                         tgaaaacaaacaa‑acCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCACGAGCTACCAACTCTTTTTCCGCAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGcca                                                                                                               <  5:95301/136‑1 (MQ=255)

                         tGCAAACAAAAAA‑ACCACCGCTACCAGCGGTGGTTTGTTTGGCGGATCAAGAGCGACCAACGCGTTTTACGAAGGTAACTGGCTTCAGCAGCGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTTGGccc                                                                                                               >  4:174432/1‑138 (MQ=255)

                          ccaaacaaaAAA‑ACCACCGCTACCAGCGGTGGATTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGccac                                                                                                              <  1:293765/138‑1 (MQ=255)

                           caaacaaaAAACACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGccac                                                                                                              <  2:50695/139‑1 (MQ=255)

                           caaacaaaAAA‑ACGACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGccacc                                                                                                             <  4:240030/139‑1 (MQ=255)

                           caaacaaaAAA‑ACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGccacc                                                                                                             <  6:284105/139‑1 (MQ=255)

                           caaacaaaAAA‑ACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGccacc                                                                                                             <  1:251071/139‑1 (MQ=255)

                           caaacaaaAAA‑ACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGccacc                                                                                                             <  1:168673/139‑1 (MQ=255)

                           caaacaaaAAA‑ACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGccacc                                                                                                             <  4:77764/139‑1 (MQ=255)

                           caaacaaaAAA‑ACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGccac                                                                                                              <  2:180527/138‑1 (MQ=255)

                            aaacaaaAAA‑ACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGccacca                                                                                                            <  7:235064/139‑1 (MQ=255)

                             aacaaaAAA‑ACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCcaccac                                                                                                           <  3:284045/139‑1 (MQ=255)

                                   aaa‑aCCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAg                                                                                                     <  2:28323/139‑1 (MQ=255)

                                   aaa‑aCCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAg                                                                                                     <  3:86961/139‑1 (MQ=255)

                                     a‑aCCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCGCCACTTCAAGaa                                                                                                   <  5:18747/139‑1 (MQ=255)

                                     a‑aCCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGaa                                                                                                   <  5:73549/139‑1 (MQ=255)

                                     a‑aCCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGaa                                                                                                   <  7:198413/139‑1 (MQ=255)

                                     a‑aCCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGaa                                                                                                   <  1:100024/139‑1 (MQ=255)

                                       accaccGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAAc                                                                                                  <  5:127672/139‑1 (MQ=255)

                                                 ccAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAATGTAACTGGCTTCAGCAGAGCGCATATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCAc                                                                                        <  3:152657/139‑1 (MQ=255)

                                                 ccAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCAc                                                                                        <  4:150667/139‑1 (MQ=255)

                                                 ccAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCAc                                                                                        <  2:258075/139‑1 (MQ=255)

                                                 ccAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCAc                                                                                        <  5:145675/139‑1 (MQ=255)

                                                 ccAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCAc                                                                                        <  1:230008/139‑1 (MQ=255)

                                                 ccAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCAc                                                                                        <  8:281585/139‑1 (MQ=255)

                                                 ccAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCAc                                                                                        <  6:189101/139‑1 (MQ=255)

                                                 ccAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGGGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCAc                                                                                        <  8:184159/139‑1 (MQ=255)

                                                 ccAGCGGTGGTTTGTTTGCCGGATCAACAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCAc                                                                                        <  5:32146/139‑1 (MQ=255)

                                                  cAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCAcc                                                                                       <  3:46737/139‑1 (MQ=255)

                                                   aGCGGTTGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCg                                                                                      <  5:294794/139‑1 (MQ=255)

                                                   aGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCg                                                                                      <  5:212382/139‑1 (MQ=255)

                                                   aGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCg                                                                                      <  2:98595/139‑1 (MQ=255)

                                                      ggtggtTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGcc                                                                                    >  1:77356/1‑138 (MQ=255)

                                                        tggtTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTAc                                                                                 <  6:162522/139‑1 (MQ=255)

                                                        tggtTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTAc                                                                                 <  1:53953/139‑1 (MQ=255)

                                                          gtttgtttGCCGGCTCAAGAGCTACCAACTATTTTTGCGAAGGTAACTGGCTTAAGCATCTCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTTGGCCACCACGTAAACAACTCAGTTGCACCGCCTACAt                                                                               >  4:195490/1‑139 (MQ=255)

                                                                 tgCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCg                                                                         <  3:255114/138‑1 (MQ=255)

                                                                 tgCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCg                                                                         <  7:84608/138‑1 (MQ=255)

                                                                 tgCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCg                                                                         <  1:209498/138‑1 (MQ=255)

                                                                  gCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGc                                                                        <  4:313279/138‑1 (MQ=255)

                                                                  gCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGc                                                                        <  7:106231/138‑1 (MQ=255)

                                                                  gCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGc                                                                        <  8:75793/138‑1 (MQ=255)

                                                                           agagCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATc                                                              <  3:19358/139‑1 (MQ=255)

                                                                                 aCCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGATCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACATCTCTCTGCGAATCCTGTTa                                                        >  8:110867/1‑139 (MQ=255)

                                                                                 aCCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAgtg                                                                                                                                <  1:273545/67‑1 (MQ=255)

                                                                                 aCCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGcca                                                                                                               >  5:231010/1‑84 (MQ=255)

                                                                                 aCCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGaa                                                                                                   <  4:108585/96‑1 (MQ=255)

                                                                                 aCCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTa                                                        >  5:104297/1‑139 (MQ=255)

                                                                                       tctTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCACATACCAAAAACTGTTCTTCTAGTCTAGCCGTAGTTAGGCCACCACTTCAAGACCTCTCTAGCACAGCCTACATACCTCGCTCTCCTAATACTGTTACCAGTg                                                  >  8:4979/1‑139 (MQ=255)

                                                                                          ttttCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGcc                                                                                                                >  1:31319/1‑74 (MQ=255)

                                                                                          ttttCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAg                                                                                                     <  6:215702/85‑1 (MQ=255)

                                                                                          ttttCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCAc                                                                                        >  5:163287/1‑98 (MQ=255)

                                                                                                 aaGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCtgctgc                                          >  3:233679/1‑137 (MQ=255)

                                                                                                 aaGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCa                                        <  6:138879/139‑1 (MQ=255)

                                                                                                  aGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAg                                       <  8:256520/139‑1 (MQ=255)

                                                                                                    gTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCTAGTg                                     <  6:87416/139‑1 (MQ=255)

                                                                                                       aCTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTCGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGc                                   >  6:239813/1‑138 (MQ=255)

                                                                                                        cTGGCTTCAGCAGAGCGCAGATAACAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGa                                 <  7:274069/139‑1 (MQ=255)

                                                                                                              tCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAg                                                                                                     <  1:119449/65‑1 (MQ=255)

                                                                                                              tCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAg                                       >  6:262586/1‑127 (MQ=255)

                                                                                                              tCAGCAGACCGCAGATACCAAAAACTGTTCTTCTAGTGTAGCCCTAGTTAGGCCACCACTGCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCACTGGCTGCTGCCAGTGGCGAGAAGTc                           >  2:231886/1‑139 (MQ=255)

                                                                                                               cagcagAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGc                                                                   <  7:173660/98‑1 (MQ=255)

                                                                                                               cagcagAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCTATAAGTc                           <  3:305731/138‑1 (MQ=255)

                                                                                                               cagcagAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGt                            <  2:101742/137‑1 (MQ=255)

                                                                                                                agcagAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCgt                         <  6:30280/139‑1 (MQ=255)

                                                                                                                 gcagAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCgtg                        <  7:65303/139‑1 (MQ=255)

                                                                                                                     agCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGc                                                                        <  3:214832/87‑1 (MQ=255)

                                                                                                                      gcgcAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGACACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTTCTGCCAGTGGCGATAAGTCGTGTCTTa                   <  7:150445/139‑1 (MQ=255)

                                                                                                                       cgcAGATCCCAAATACTCTTCTTCTAGTGTAGCCGTAGTTAGGCCACCAATTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGGCAGAGGCCATAAGTCGTGTcattc                  >  7:215343/1‑135 (MQ=255)

                                                                                                                       cgcAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGccac                                                                                                              <  7:44175/47‑1 (MQ=255)

                                                                                                                       cgcAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCTCCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCgt                         >  1:187807/1‑132 (MQ=255)

                                                                                                                       cgcAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTAc                  <  3:173789/139‑1 (MQ=255)

                                                                                                                       cgcAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTAc                  >  2:307517/1‑139 (MQ=255)

                                                                                                                       cgcAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTAc                  >  4:22076/1‑139 (MQ=255)

                                                                                                                       cgcAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTAc                  >  8:309174/1‑139 (MQ=255)

                                                                                                                       cgcAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTAc                  <  4:290010/139‑1 (MQ=255)

                                                                                                                       cgcAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTAc                  >  6:115560/1‑139 (MQ=255)

                                                                                                                       cgcAGATACCAAATACTGTTCGTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTAc                  <  4:265592/139‑1 (MQ=255)

                                                                                                                         cAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACAGCCTACATACCTCGCTCTGCAAATCCAGTTACAAGAGGCTGCTCCAAGTGGCGATAAGTCGTGGGTTAcag                >  1:142732/1‑137 (MQ=255)

                                                                                                                            aTACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGc                                                                        >  8:230531/1‑80 (MQ=255)

                                                                                                                                cAAATACTGTTCTTCTAGTGTAGCCGTAGGTAGGCCACCACTTCAAGAACTCTGTAGCCCCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGa         <  6:66438/139‑1 (MQ=255)

                                                                                                                                 aaaTACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGGCTTTCCGGGTTGGAc        >  8:138478/1‑139 (MQ=255)

TTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAA‑ACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGA  >  pSIJ663_pHT315_araE_xy/4912‑5184

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`