Predicted mutation
evidence seq id position mutation freq annotation gene description
RA BS168_reference_genome 335,553 T→A 8.3% L75L (CTT→CTA)  ycgG → conserved protein of unknown function (similar to phage protein)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*BS168_reference_genome335,5530TA 8.3% 139.5 / 3.1 48L75L (CTT→CTA) ycgGconserved protein of unknown function (similar to phage protein)
Reads supporting (aligned to +/- strand):  ref base T (16/28);  new base A (2/2);  total (18/30)
Fisher's exact test for biased strand distribution p-value = 6.24e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.41e-01

GCAGAAACTCTGGGGGACGACGCGGATACATTTCCGTGCATTCCCGGACGCCAGGCGTTTCTGACTGATCAGCTTCGGATTGCTTTTGCCGGAGATCCGCGGGAAAACCGCACAGCGGAGGAACTGGCTCCGTTGCTTGCGGAGTACGGCAAGATATCGCGGGACACGGGAAAATACGCATCCCTCGTTGTGTTGTTTGATACACCGGAAGATTTGGCGGAGCATTATTCGATTGAAGCGTATGAAGAGCTGTTTTGGCGCTTTTTAAATAGA  >  BS168_reference_genome/335416‑335688
                                                                                                                                         |                                                                                                                                       
gCAGAAACTCTGGGGGACGACGCGGATACATTTCCGTGCATTCCCGGACGCCAGGCGTTTCTGACTGATCAGCTTCGGATTGCTTTTGCCGGAGATCCGCGGGAAAACCGCACAGCGGAGGAACTGGCTCCGttgcttg                                                                                                                                        <  2:194970/139‑1 (MQ=255)
gCAGAAACTCTGGGGGACGACGCGGATACATTTCCGTGCATTCCCGGACGCCAGGCGGTTCTGACTGATCAGCTTCGGATTGCTTTTGCCGGAGATCCGCGGGAAAACCGCACAGCGGAGGAACTGGCTCCGttgcttg                                                                                                                                        <  5:248009/139‑1 (MQ=255)
     aaCTCTGGGGGACGACGCGGATACATTTCCGTGCATTCCCGGACGCCAGGCGTTTCTGACTGATCAGCTTCGGATTGCTTTTGCCGGAGATCCGCGGGAAAACCGCACAGCGGAGGAACTGGCTCCGTTGCTTGCGGAg                                                                                                                                   <  8:93473/139‑1 (MQ=255)
          tGGGGGACGACGCGGATACATTTCCGTGCATTCCCGGACGCCAGGCGTTTCTGTCTGATCAGCTTCGGATTGCTTTTGCCGGAGATCCGCGGGAAAACCGCACAGCGGAGGAACTGGCTCCGTTGCTTGCGGAGTACgg                                                                                                                              <  4:251247/139‑1 (MQ=255)
          tGGGGGACGACGCGGATACATTTCCGTGCATTCCCGGACGCCAGGCGTTTCTGACTGATCAGCTTCGGATTGCTTTTGCCGGAGATCCGCGGGAAAACCGCACAGCGGAGGAACTGGCTCCGTTGCTTGCg                                                                                                                                      >  8:267639/1‑131 (MQ=255)
          tGGGGGACGACGCGGATACATTTCCGTGCATTCCCGGACGCCAGGCGTTTCTGACTGATCAGCTTCGGATTGCTTTTGCCGGAGATCCGCGGGAAAACCGCACAGCGGAGGAACTGGCTCCGTTGCTTGCg                                                                                                                                      <  7:267639/131‑1 (MQ=255)
               gacgacGCGGATACATATCCGTGCATTCCCGGACGCCAGGCGTTTCTGACTGATCAGCTTCGGATTGCTTTTGCCGGAGATCCGCGGGAAAACCGCACAGCGGAGGAACTGGCTCCGTTGCTTGCGGAGTACGGCAAGa                                                                                                                         <  1:8409/139‑1 (MQ=255)
                  gacgCGGATACATTTCCGTGCATTCCCGGACGCCAGGCGTTTCTGACTGATCAGCTTCGGATTGCTTTTGCCGGAGATCCGCGGGAAAACCGCACAGCGGAGGAACTGGCTCCGTTGCTTGCGGAGTACGGCAAGatat                                                                                                                      >  4:142525/1‑139 (MQ=255)
                         aTACATTTCCGTGCATTCCCGGACGCCAGGCGTTTCTGACTGATCAGGTTAGGATTGCTTTTGCCGGAGATACGAGGGAAAACCCGACAGCGGAGGAACTGGCTCAGTTGCTTGCGGAGTACGGCACGATATCTCgggt                                                                                                               >  8:9564/1‑138 (MQ=255)
                         aTACATTTCCGTGCATTCCCGGACGCCAGGCGTTTCTGACTGATCAGCTTCGGATTGCTTTTGCCGGAGATCCGCGGGAAAACCGCACAGCGGAGGAACTGGCTCCGTTGCTTGCGGAGTACGGCAAGATATCGCGGGa                                                                                                               <  1:118282/139‑1 (MQ=255)
                         aTACATTTCCGTGCATTCCCGGACGCCAGGCGTTTCTGACTGATCAGCTTCGGATTGCTTTTGCCGGAGATCCGCGGGAAAACCGCACAGCGGAGGAACTGGCTCCGTTGCTTGCGGAGTACGGCAAGATATCGCGGGa                                                                                                               <  5:71917/139‑1 (MQ=255)
                         aTACATTTCCGTGCATTCCCGGACGCCAGGCGTTTCTGACTGATCAGCATCGGATTGCTTTTGCCGGAGAACCGCGGGAAAACCGCACAGCGGAGGAACTGGCTCCGTTGCTTGCGGAGTACGGCAAGTTATCGCGGGa                                                                                                               <  7:9564/139‑1 (MQ=255)
                           aCATTTCCGTGCATTCCCGGACGCCAGGCGTTTCTGACTGATCAGCTTCGGATTGCTTTTGCCGGAGATCCGCGGGAAAACCGCACAGCGGAGGAACTGGCTCCGTTGCTTGCGGAGTACGGCAAGATATCGCGGGaca                                                                                                             <  7:293521/139‑1 (MQ=255)
                           aCATATCCGTGCATTCCCGGACGCCAGGCGTTTCTGACTGATCAGCTTCGGATTGCTTTTGCCGGAGATCCGCGGGAAAACCGCACAGCGGAGGAACTGGCTCCGTTGCTTGCGGAGTACGGCAAGATATCGCGGGaca                                                                                                             <  1:143717/139‑1 (MQ=255)
                            cATTTCCGTGCATTCCCGGACGCCAGGCGTTTCTGACTGATCAGCTTCGGATTGCTTTTGCCGGAGATCCGCGGGAAAACCGCACAGCGGAGGAACTGGCTCCGTTGCTTGCGGAGTACGGCAAGATATCGCGGGaca                                                                                                             <  1:162626/138‑1 (MQ=255)
                                 ccGTGCATTCCCGGACGCCAGGCGTTTCTGACTGATCAGCTTCGGATTGCTTTTGCCGGAGATCCGCGGGAAAACCGCACAGCGGAGGAACTGGCTCCGTTGCTTGCGGAGTACGGCAAGATATCGCGGGACACGGGa                                                                                                        <  7:58339/138‑1 (MQ=255)
                                 ccGTGCATTCCCGGACGCCAGGCGTTTCTGACTGATCAGCTTCGGATTGCTTTTGCCGGAGATCCGCGGGAAAACCGCACAGCGGAGGAACTGGCTCCGTTGCTTGCGGAGTACGGCAAGATATCGCGGGACACGGGa                                                                                                        >  4:157488/1‑138 (MQ=255)
                                      cATTCCCGGACGCCAGGCGTTTCTGACTGATCAGCTTCGGATTGCTTTTGCCGGAGATCCGCGGGAAAACCGCACAGCGGAGGAACTGGCTCCGTTGCTTGCGGAGTACGGCAAGATATCGCGGGACACGGGAAAATAc                                                                                                  >  7:94296/1‑139 (MQ=255)
                                        ttCCCGGACGCCAGGCGTTTCTGACTGATCAGCTTCGGATTGCTTTTGCCGGAGATCCGCGGGAAAACCGCACAGCGGAGGAACTGGCTCCGTTGCTTGCGGAGTACGGCAAGATATCGCGGGACACGGGAAAATACGc                                                                                                <  3:21240/139‑1 (MQ=255)
                                             ggACGCCAGGCGTTTCTGACTGATCAGCTTCGGATTGCTTTTGCCGGAGATCCGCGGGAAAACCGCACAGCGGAGGAACTGGCTCCGTTGCTTGCGGAGTACGGCAAGATATCGCGGGACACGGGAAAATACGCATccc                                                                                           <  1:282220/139‑1 (MQ=255)
                                                cGCCAGGCGTTTCTGACTGATCAGCTTCGGATTGCTTTTGCCGGAGATCCGCGGGAAAACCGCACAGCGGAGGAACTGGCTCCGTTGCTTGCGGAGTACGGCAAGATATCGCGGGACACGGGAAAATACGCATCCCTCg                                                                                        <  4:307534/139‑1 (MQ=255)
                                                cGCCAGGCGTTTCTGACTGATCAGCTTCGGATTGCTTTTGCCGGAGATCCGCGGGAAAACCGCACAGCGGAGGAACTGGCTCCGTTGCTTGCGGAGTACGGCAAGATATCGCGGGACACGGGAAAATACGCATCCCTCg                                                                                        <  6:287939/139‑1 (MQ=255)
                                                  ccAGGCGTTTCTGACTGATCAGCTTCGGATTGCTTTTGCCGGAGATCCGCGGGAACACCGCACAGCGGAGGAACTGGCTCCGTTGCTTGCGGAGTACGGCAAGATATCGCGGGACACGGGAAAATACGCATCCCTCgtt                                                                                      <  2:272773/139‑1 (MQ=255)
                                                     ggCGTTTCTGACTTATCAGCTTCGGATTGCTTTTGCCGGAGATCCGCGGGAAAACCGCTCAGCGGAGGAACTGGCTCCGTTGCTTGCGGAGTACGGCAAGATATCGCGGGACACGGGAAAATACGCATCCCTCgttgtg                                                                                   <  7:300053/139‑1 (MQ=255)
                                                     ggCGTTTCTGACTGATCAGCTTCGGATTGCTTTTGCCGGAGATCCGCGGGAAAACCGCACAGCGGAGGAACTGGCTCCGTTGCTTGCGGAGTACGGCAAGATATCGCGGGACACGGGAAAATACGCATCCCTCgttgtg                                                                                   <  2:298111/139‑1 (MQ=255)
                                                               actgaTCAGCTTCGGATTGCTTTTGCCGGAGATCCGCGGGAAAACCGCACAGCGGAGGAACTGGCTCCGTTGCTTGCGGAGTACGGCAAGATATCGCGGGACACGGGAAAATACGCATCCCTCGTTGTGTTGTTTGATa                                                                         >  7:55102/1‑139 (MQ=255)
                                                               actgaTCAGCTTCGGATTGCTTTTGCCGGAGATCCGCGGGAAAACCGCACAGCGGAGGAACTGGCTCCGTTGCTTGCGGAGTACGGCAAGATATCGCGGGACACGGGAAAATACGCATCCCTCGTTGTGTTGTTTGATa                                                                         <  8:300196/139‑1 (MQ=255)
                                                                                tGCTTTTGCCGGAGCTCCGCGGGAAACCCGCACCGCGGAGGAACTGGCTCCGTTGCTTGCGGAGTACGGCAAGATATCGCGGGACACGTGAAAATACGCATCCCTCGTTGTGTTGTTTGATACACCGGAAGATTTGGCg                                                        <  3:159291/139‑1 (MQ=255)
                                                                                 gCTTTTGCCGGAGATCCGCGGGAAAACCGCACAGCGGAGGAACTGGCTCCGTTGCTTGCGGAGTACGGCAAGATATCGCGGGACACGGGAAAATACGCATCCCTCGTTGTGTTGTTTGATACACCGGAAGATTTGGCg                                                        <  8:94296/138‑1 (MQ=255)
                                                                                 gCTTTTGCCGGAGATCCGCGGGAAAACCGCACAGCGGAGGAACTGGCTCCGTTGCTTGCGGAGTACGGCAAGATATCGCGGGACACGGGAAAATACGCATCCCTCGTTGTGTTGTTTGATACACCGGAAGATTTGGCg                                                        <  7:249175/138‑1 (MQ=255)
                                                                                     ttGCCGGAGATCCGCGGGAAAACCGCACAGCGGAGGAACTGGCTCCGTTGCTTGCGGAGTACGGCAAGATATCGCGGGACACGGGAAAATACGCATCCCTCGTTGTGTTGTTTGATACACCGGAAGATTTGGCGGAGCa                                                   >  4:83053/1‑139 (MQ=255)
                                                                                        ccGGAGATGGGCGGGATTACCGAAGAGAGGAGGTTCTGGCTCGGTTGCTTGGTGAGTTCGGCAATATATGGCTTGACTCGGGAAAATAGGCATGCCTCGTAGTGTTGTTTGATACACCGGTATATTTGTAGGAgatttt                                                <  4:137123/139‑6 (MQ=255)
                                                                                        ccGGAGATCAGCGGGAAAACCGCACAGCGGAGGAACTGGCTCCGTTGCTTGCGGAGTACGGCAAGATATCGCGGGACACGGGAAAATACGCATCCCTCGTTGTGTTGTTTGATACACCGGAAGATTTGGCGGAGCatta                                                <  7:193060/139‑1 (MQ=255)
                                                                                            agaTCCGCGGGAAAACCGCACAGCGGAGGAACTGGCTCCGTTGATTGCGGAGTACGGCAAGATATCGCGGGACACGGGAAAATACGCATCCCTCGTTGTGTTGTTTGATACACCGGAAGATTAGGCGGAGCATTATTc                                             >  3:83270/1‑138 (MQ=255)
                                                                                                 cgcgTGAAAACCTCACAGCTGAGGCACTTGCTCCGTTGCGTGCGGAGTAAGGCAAGATCTAGCCTGACACGGGAAAATACGCATCCCACGTTGTGTCGTTTGATACACCGGAAGATTTTGCGGAGCATTATTCGATTGa                                       <  4:26420/139‑1 (MQ=255)
                                                                                                                     gaggaACTGGCTCCGTTGCTAGCGGAGTACGGCAAGATATCGCGGGACACGGGAAAATACGCATCCCTCGTTGTGTTGTTTGATACACCGGAAGATTTGGCGGAGCATTATTCGATTGAAGCGTATGAAGAGCTGtttt                   >  7:288438/1‑139 (MQ=255)
                                                                                                                     gaggaACTGGCTCCGTTGCTAGCGGAGTACGGCAAGATATCGCGGGACACGGGAAAATACGCATCCCTCGTTGTGTTGTTTGATACACCCGAAGATTTGGCGGAGCATTATTCGATTGAAGCGTATGAAGAGCTGtttt                   >  7:284391/1‑139 (MQ=255)
                                                                                                                      aggaACTGGCTCCGTTGCTTGCGGAGTACGGCAAGATATCGCGGGACACGGGAAAATACGCATCCCTCGTTGTGTTGTTTGATACACCGGAAGATTTGGCGGAGCATTATTCGATTGAAGCGTATGAAGAGCTGTTTTg                  >  7:6001/1‑139 (MQ=255)
                                                                                                                        gaACTGGCTCCGTTTCTAGCGGAGTACTGCAAGATATCGCGGGACACGTGAAAATACGCATCCCTCGTTGTGTTGTTTGATACACCGGAAGATTTGGCGGAGCATTATTCGATTGAAGCGTATGAAGAGCTGTTTTGgc                <  8:284391/139‑1 (MQ=255)
                                                                                                                        gaACTGGCTCCGTTGCTTGCGGAGTACGGCAAGATATCGCGGGACACGGGAAAATACGCATCCCTCGTTGTGTTGTTTGATACACCGGAAGATTTGGCGGAGCATTATTCGATTGAAGCGTATGAAGAGCTGTTTTGgc                <  3:310092/139‑1 (MQ=255)
                                                                                                                        gaACTGGCTCCGTTGCTTGCGGAGTACGGCAAGATATCGCGGGACACGGGAAAATACGCATCCCTCGTTGTGTTGTTTGATACACCGGAAGATTTGGCGGAGCATTATTCGATTGAAGCGTATGAAGAGCTGTTTTGgc                <  3:142525/139‑1 (MQ=255)
                                                                                                                        gaACTGGCTCCGTTGCTAGCGGAGTACGGCAAGATATCGCGGGACACGGGAAAATACGCATCCCTCGTTGTGTTGTTTGATACACCGGAAGATTTGGCGGAGCATTATTCGATTGAAGCGTATGAAGAGCTGTTTTGgc                <  8:288438/139‑1 (MQ=255)
                                                                                                                             ggCTCCGTTGCTTGCGGAGTACGGCAAGATATCGCGGGACACGGGAAAATACGCATCCCTCGTTGTGTTGTTTGATa                                                                         >  1:139421/1‑77 (MQ=255)
                                                                                                                             ggCTCCGTTGCTTGCGGAGTACGGCAAGATATCGCGGGACACGGGAAAATACGCATCCCTCGTTGTGTTGTTTGATa                                                                         <  2:139421/77‑1 (MQ=255)
                                                                                                                             ggCTCCGTTGCTTGCGGAGTACGGCAAGATATCGCGGGACACGGGAAAATACGCATCCCTCGTTGTGTTGTTTGATACACCGGAAGATTTGGCGGAGCATTATTCGATTGAAGCGTATGAAGAGCTGTTTTGGCGCttt           >  6:260956/1‑139 (MQ=255)
                                                                                                                             ggCTCCGTTGCTTGCGGAGTACGGCAAGATATCGCGGGACACGGGAAAATACGCATCCCTCGTTGTGTTGTTTGATACACCGGAAGATTTGGCGGAGCATTATTCGATTGAAGCGTATGAAGAGCTGTTTTGGCGCttt           >  5:40743/1‑139 (MQ=255)
                                                                                                                             ggCTCCGTTGCTTGCGGAGTACGGCAAGATATCGCGGGACACGGGAAAATACGCATCCCTCGTTGTGTTGTTTGATACACCGGAAGATTTGGCGGAGCATTATTCGATTGAAGCGTATGAAGAGCTGTTTTGGCGCttt           >  3:272494/1‑139 (MQ=255)
                                                                                                                             ggCTCCGTTGCTTGCGGAGTACGGAAAGATATAGCGGGACACGGGAAAATACTCATCCCTCGTTGTGTGGTTTGATACACCGGAAGATTTGGCGGAGCATTATTCGATTGAAGCTTATGAAGAGCTGTTTTGGCGCttt           >  7:158024/1‑139 (MQ=255)
                                                                                                                                tCCGTTGCTTGCGGAGTACGGCAAGATATCGCGGGACACGGGAAAATACGCATCCCTCGTTGTGTTGTTTGATACACCGGAAGATTTGGCGGAGCATTATTCGATTGAAGCGTATGAAGAGCTGTTTTGGCGCttttt         >  2:123773/1‑138 (MQ=255)
                                                                                                                                   gTTGCTTGCGGAGTACGCCAAGAAATCGCGGGACACGGGAAAATACGCATCCCTCGATCTGTTGATTGATACACAGGAAGATTTGGCGGAGCATTATTCGATAGAAGCGTATGAAGAGCTGTTTTGGCGCTTTTTaaa      >  7:28465/1‑138 (MQ=255)
                                                                                                                                       cttgcGGAGTACGGCAAGATATCGCGGGACACGGGAAAATACGCATCCCTCGTTGTGTTGTTTGATACACCGGAAGATTTGGCGGAGCATTATTCGATTGAAGCGTATGAAGAGCTGTTTTGGCGCTTTTTAAATAGa  <  8:55102/138‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                       
GCAGAAACTCTGGGGGACGACGCGGATACATTTCCGTGCATTCCCGGACGCCAGGCGTTTCTGACTGATCAGCTTCGGATTGCTTTTGCCGGAGATCCGCGGGAAAACCGCACAGCGGAGGAACTGGCTCCGTTGCTTGCGGAGTACGGCAAGATATCGCGGGACACGGGAAAATACGCATCCCTCGTTGTGTTGTTTGATACACCGGAAGATTTGGCGGAGCATTATTCGATTGAAGCGTATGAAGAGCTGTTTTGGCGCTTTTTAAATAGA  >  BS168_reference_genome/335416‑335688

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑