Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	BS168_reference_genome	770,241	G→T	7.7%	R3M (AGG→ATG)	rhgW →	rhamnogalacturonan endolyase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	BS168_reference_genome	770,241	0	G	T	7.7%	157.6 / 3.9	52	R3M (AGG→ATG)	rhgW	rhamnogalacturonan endolyase
Reads supporting (aligned to +/- strand):  ref base G (19/29);  new base T (2/2);  total (21/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.82e-01

CCGGGTAACAAAAAAATGACAAATAAGCAAAAAAGTGCAGTGTCCGAAATAGGCAGATGCAAAAAAGTGAAATTGAAGACAGCTCACAAAGCGCATAAAATCCAATTTAAGTAAGCGCTTACAGATAAATGAGAAGGAGCTGTCTGATGATTAGACGAAGGAAACGCATGTTTACCGCTGTTACGTTGCTGGTCTTGTTGGTGATGGGAACCTCTGTATGTCCTGTGAAAGCTGAAGGGGCAGCGCGGCAGATGGAAGCGCTGAACCG  >  BS168_reference_genome/770106‑770373                                                                                                                                        |                                                                                                                                     ccGGGTAACAAAAAAATGACAAATAAGCAAAAAAGTGCAGTGTCCGAAATAGGCAGATGCAAAAAAGTGAAATTGAAGACAGCTCACAAAGCGCATAAAATCCAATTTAAGTAAGCGCTTACAGATAAATGAGAAGGAg                                                                                                                                   <  7:257579/139‑1 (MQ=255) ccGGGTAACAAAAAAATGACAAATAAGCAAAAAAGTGCAGTGTCCGAAATAGGCAGATGCAAAAAAGTGAAATTGAAGACAGCTCACAAAGCGCATAAAATCCAATTTAAGTAAGCGCTTACAGATAAATGAGAAGGAg                                                                                                                                   <  7:275105/139‑1 (MQ=255) ccGGGTAACAAAAAAATGACAAATAAGCAAAAAAGTGCAGTGTCCGAAATAGGCAGATGCAAAAAAGTGAAATTGAAGACAGCTCACAAAGCGCATAAAATCCAATTTAAGTAAGCGCTTACAGATAAATGAGAAGGAg                                                                                                                                   <  7:52407/139‑1 (MQ=255) ccGGGTAACAAAAAAATGACAAATAAGCAAAAAAGTGCAGTGTCCGAAATAGGCAGATGCAAAAAAGTGAAATTGAAGACAGCTCACAAAGCGCATAAAATCCAATTTAAGTAAGCGCTTACAGATAAATGAGAAGGAg                                                                                                                                   <  4:112679/139‑1 (MQ=255)    ggTAACAAAAAAATGACAAATAAGCAAAAAAGTGCAGTGTCCGAAATAGGCAGATGCAAAAAAGTGAAATTGAAGACAGCTCACAAAGCGCATAAAATCCAATTTAAGTAAGCGCTTACAGATAAATGAGAAGGAGCTg                                                                                                                                >  6:8298/1‑139 (MQ=255)             aaaaTGACAAATAAGCAAAAAAGTGCAGTGTCCGAAATAGGCAGATGCAAAAAAGTGAAATTGAAGACAGCTCACAAAGCGCATAAAATCCAATTTAAGTAAGCGCTTACAGATAAATGAGAAGGAGCTGTCTgatgat                                                                                                                       <  1:27024/139‑1 (MQ=255)                          aGCAAAAAAGTGCAGTGTCCGAAATAGGCAGATGCAAAAAAGTGAAATTGAAGACAGCTCACAAAGCGCATAAAATCCAATTTAAGTAAGCGCTTACAGATAAATGAGAAGGAGCTGTCTGATGATTAGACGAAGGaaa                                                                                                          <  2:235936/139‑1 (MQ=255)                               aaaaGTGCAGTGTCCGAAATAGTCAGATGAAAAAAAGTGAAATTGAAGACATCTCACAAAGCGCATAAAATCCAATTTAAGTAAGCGCTTACTGATAAATGAGAAGGAGCTGTCTGATGATTAGACGAAGGAAACTCAt                                                                                                     <  5:264427/139‑1 (MQ=255)                                           tCCGAAATAGGCAGATGCAAAAAAGTGAAATTGAAGACAGCTCACAAAGCGCATAAAATCCAATTTAAGTAAGCGCTTACAGATAAATGAGAAGGAGCTGTCTGATGATTAGACGAAGGAAACGCATGTTTACCGCTGt                                                                                         >  8:89374/1‑139 (MQ=255)                                           tCCGAAATAGGCAGATGCAAAAAAGTGAAATTGAAGACAGCTCACAAAGCGCATAAAATCCAATTTAAGTAAGCGCTTACAGATAAATGAGAAGGAGCTGTCTGATGATTAGACGAAGGAAACGCATGTTTACCGCTGt                                                                                         >  3:290006/1‑139 (MQ=255)                                                    ggCAGATGCAAAAAAGTGAAATTGAAGACAGCTCACAAAGCTCATAAAATCCAATTTAAGTAAGCGCTTACAGATAAATGAGAAGGAGCTGTCTGATGATTAGACGAAGGAAACGCATGTTTACCGCTGTTACGTTGCt                                                                                <  5:219157/139‑1 (MQ=255)                                                    ggCAGATGCAAAAAAGTGAAATTGAAGACAGCTCACAAAGCGCATAAAATCCAATTTAAGTAAGCGCTTACAGATAAATGAGAAGGAGCTGTCTGATGATTAGACGAAGGAAACGCATGTTTACCGCTGTTACGTTGCt                                                                                <  1:288068/139‑1 (MQ=255)                                                     gCAGATGCAAAAAAGTGAAATTGAAGACAGCTCACAAAGCGCATAAAATCCAATTTAAGTAAGCGCTTACAGATAAATGAGAAGGAGCTGTCTGATGATTAGACGAAGGAAACGc                                                                                                       <  1:104760/115‑1 (MQ=255)                                                     gCAGATGCAAAAAAGTGAAATTGAAGACAGCTCACAAAGCGCATAAAATCCAATTTAAGTAAGCGCTTACAGATAAATGAGAAGGAGCTGTCTGATGATTAGACGAAGGAAACGc                                                                                                       <  7:79648/115‑1 (MQ=255)                                                     gCAGATGCAAAAAAGTGAAATTGAAGACAGCTCACAAAGCGCATAAAATCCAATTTAAGTAAGCGCTTACAGATAAATGAGAAGGAGCTGTCTGATGATTAGACGAAGGAAACGc                                                                                                       >  2:104760/1‑115 (MQ=255)                                                     gCAGATGCAAAAAAGTGAAATTGAAGACAGCTCACAAAGCGCATAAAATCCAATTTAAGTAAGCGCTTACAGATAAATGAGAAGGAGCTGTCTGATGATTAGACGAAGGAAACGc                                                                                                       >  8:79648/1‑115 (MQ=255)                                                     gCAGATGCAAAAAAGTGAAATTGAAGACAGCTCACAAAGCGCATAAAATCCAATTTAAGTAAGCGCTTACAGATAAATGAGAAGGAGCTGTCTGATGATTAGACGAAGGAAACGCATGTTTACCGCTGTTACGTTGCTg                                                                               >  8:258525/1‑139 (MQ=255)                                                                aaaGTGAAATTGAAGACATCTCACAAAGCGCATAAAATCCAATTTAAGTAAGCGCTTACAGATAAATGAGAAGGAGCTGTCTGATGATTAGACGAAGGAAACGCATGTTTACCGCTGTTACGTTGCTGGTCTTGTTGGt                                                                    <  7:258525/139‑1 (MQ=255)                                                                       aaTTGAAGACAGCTCACAAAGCGCATAAAATCCAATTTAAGTAAGCGCTTACAGATAAATGAGAAGGAGCTGTCTGATGATTAGACGAAGGAAACGCATGTTTACCGCTGTTACGTTGCTGGTCTTGTTGGTGATggg                                                              <  4:119178/138‑1 (MQ=255)                                                                       aaTTGAAGACAGCTCACAAAGCGCATAAAATCCAATTTAAGTAAGCGCTTACAGATAAATGAGAAGGAGCTGTCTGATGATTAGACGAAGGAAACGCATGTTTACCGCTGTTACGTTGCTGGTCTTGTTGGTGATggg                                                              <  5:163639/138‑1 (MQ=255)                                                                         ttGAAGACAGCTCACAAAGCGCATAAAATCCAATTTAAGTAAGCGCTTACAGATAAATGAGAAGGAGCTGTCTGATGATTAGACGAAGGAAACGCATGTTTACCGCTGTTACGTTGCTGGTCTTGTTGGTGATGGGAAc                                                           <  8:150996/139‑1 (MQ=255)                                                                         ttGAAGACAGCTCACAAAGCGCATAAAATCCAATTTAAGTAAGCGCTTACAGATAAATGAGAAGGAGCTGTCTGATGATTAGACGAAGGAAACGCATGTTTACCGCTGTTACGTTGCTGGTCTTGTTGGTGATGGGAAc                                                           <  1:65995/139‑1 (MQ=255)                                                                         ttGAAGACAGCTCACAAAGCGCATAAAATACAATTTAAGTAAGCGCTTACAGATAAATGAGAAGGAGCTGTCTGATGATTAGACGAAGGAAACGCATGTTTACCGCTGTTACGTTGCTGGTCTTGTTGGTGATGGGAAc                                                           <  7:89374/139‑1 (MQ=255)                                                                         atGAACACAGCTCACAAAGCGCATAAAATCCAATTAAAGTAAGCGCTTACAGATAAATCAGAAGGAGCTGTCTGATGATTAGACGAAGGAAACGCATGTTTACCGCTGTTACGTTGCTGGTCTTGTTGGTGATGGGAAc                                                           <  3:229380/138‑1 (MQ=255)                                                                               aCAGCTCACAAAGCGCATAAAATCCAATTTAAGTAAGCGCTTACAGATAAATGAGACGGAGCTGTCTGATGATTAGACGAAGGAAACGCATGTTTACCGCTGTTACGTTGCTGGTCTTGTTGGTGATGGGAACCTCTGt                                                     <  6:13515/139‑1 (MQ=255)                                                                                   cTCACAAAGCGCATAAAATCCAATTTAAGTAAGCGCTTACAGATAAATGAGATGGAGCTGTCTGATGATTAGACGAAGGAAACGCATGTTTACCGCTGTTACGTGGCTGGTCTTGTTGGTGATGGGAGCCTCTGTATGt                                                 >  1:227168/1‑139 (MQ=255)                                                                                   cTCACAAAGCGCATAAAATCCAATTTAAGTAAGCGCTTACAGATAAATGAGAAGGAGCTGTCTGATGATTAGACGAAGGAAACGCATGTTTACCGCTGTTACGTTGCTGGTCTTGTTGGTGATGGGAACCTCTGTATGt                                                 >  3:83834/1‑139 (MQ=255)                                                                                   cTCACAAAGCGCATAAAATCCAATTTAAGTAAGCGCTTACAGATAAATGAGAAGGAGCTGTCTGATGATTAGACGAAGGAAACGCATGTTTACCGCTGTTACGTTGCTGGTCTTGTTGGTGATGGGAACCTCTGTAGGt                                                 >  1:222619/1‑139 (MQ=255)                                                                                      acaAAGCGCATAAAATCCAATTTAAGTAAGCGCTTACAGATAAATGAGAAGGAGCTGTCt                                                                                                                             <  8:6376/60‑1 (MQ=255)                                                                                      acaAAGCGCATAAAATCCAATTTAAGTAAGCGCTTACAGATAAATGAGAAGGAGCTGTCt                                                                                                                             >  7:6376/1‑60 (MQ=255)                                                                                        aaaGCGCATAAAATCCAATTTAAGTAAGCGCTTACAGATAAATGAGAATGAGCTGTCTGATGATTAGACGAAGGAAACGCATGTTTACCGCTGTTACGTTGCTGGTCTTGTTGGTGATGGGAAc                                                           >  2:294313/1‑124 (MQ=255)                                                                                        aaaGCGCATAAAATCCAATTTAAGTAAGCGCTTACAGATAAATGAGAATGAGCTGTCTGATGATTAGACGAAGGAAACGCATGTTTACCGCTGTTACGTTGCTGGTCTTGTTGGTGATGGGAAc                                                           <  1:298334/124‑1 (MQ=255)                                                                                        aaaGCGCATAAAATCCAATTTAAGTAAGCGCTTACAGATAAATGAGAATGAGCTGTCTGATGATTAGACGAAGGAAACGCATGTTTACCGCTGTTACGTTGCTGGTCTTGTTGGTGATGGGAAc                                                           <  1:294313/124‑1 (MQ=255)                                                                                        aaaGCGCATAAAATCCAATTTAAGTAAGCGCTTACAGATAAATGAGAATGAGCTGTCTGATGATTAGACGAAGGAAACGCATGTTTACCGCTGTTACGTTGCTGGTCTTGTTGGTGATGGGAAc                                                           >  2:298334/1‑124 (MQ=255)                                                                                        aaaGCGCATAAAATCCAATTTAAGTAAGCGCTTACAGATAAATGAGAAGGAGCTGTCTGATGATTAGACGAAGGAAACGCATGTTTACCGCTGTTACGTTGCTGGTCTTGTTGGTGATGGGAAc                                                           >  4:227494/1‑124 (MQ=255)                                                                                        aaaGCGCATAAAATCCAATTTAAGTAAGCGCTTACAGATAAATGAGAAGGAGCTGTCTGATGATTAGACGAAGGAAACGCATGTTTACCGCTGTTACGTTGCTGGTCTTGTTGGTGATGGGAAc                                                           <  3:227494/124‑1 (MQ=255)                                                                                             gcATAAAATCCAATTTAAGTAAGCGCTTACAGATAAATGAGAAGGAGCTGTCTGATGATTAGACGAAGGAAACGCATGTTTACCGCTGTTACGTTGCTGGTCTTGTTGGTGATGGGAACCTCTGTATGTCCTGTGAAAg                                       >  1:140418/1‑139 (MQ=255)                                                                                                       cAATTTAAGTAAGCGCTTACAGATAAATGAGAAGGAGCTGTCTGATGATTAGACGAAGGAAACGCATGTTTACCGCTGTTACGTTGCTGGTCTTGTTGGTGATGGGAACCTCTGTATGTCCTGTGAAAGCTGAAgggg                              <  2:140418/138‑1 (MQ=255)                                                                                                                    cgcTTACAGATAAATGAGAAGGAGCTGTCTGATGATTAGACGAAGGAAACGCATGTTTACCGCTGTTACGTTGCTGGTCTTGTTGGTGATGGGAACCTCTGTATGTCCTGTGAAAGCTGAAGGGGCAGCGCGGCAGCTg                >  2:197018/1‑139 (MQ=255)                                                                                                                        tACAGATAAATGAGAAGGAGCTGTCTGATGATTAGACGAAGGAAACGCATGTTTACCGCTGTTACGTTGCTGGTCTTGTTGGTGATGGGAACctc                                                        <  4:32101/95‑1 (MQ=255)                                                                                                                        tACAGATAAATGAGAAGGAGCTGTCTGATGATTAGACGAAGGAAACGCATGTTTACCGCTGTTACGTTGCTGGTCTTGTTGGTGATGGGAACctc                                                        >  3:32101/1‑95 (MQ=255)                                                                                                                        tACAGATAAATGAGAAGGAGCTGTCTGATGATTAGACGAAGGAAACGCATGTTTACCGCTGTTACGTTGCTGGTCTTGTTGGTGATGGGAACCTCTGTATGTCCTGTGAAAGCTGAAGGGGCAGCGCgg                      >  1:55235/1‑129 (MQ=255)                                                                                                                                aaTGAGAAGGAGCTGTCTGATGATTAGACGAAGGAAACGCATGTTTACCGCTGTTACGTTGCTGGt                                                                             <  7:163833/66‑1 (MQ=255)                                                                                                                                aaTGAGAAGGAGCTGTCTGATGATTAGACGAAGGAAACGCATGTTTACCGCTGTTACGTTGCTGGt                                                                             >  8:163833/1‑66 (MQ=255)                                                                                                                                aaTGAGAAGGAGCTGTCTGATGATTAGACGAAGGAAACGCATGTTTACCGCTGTTACGTTGCTGGTCTTGTTGGTGATGGGAAcc                                                          >  7:282414/1‑85 (MQ=255)                                                                                                                                aaTGAGAAGGAGCTGTCTGATGATTAGACGAAGGAAACGCATGTTTACCGCTGTTACGTTGCTGGTCTTGTTGGTGATGGGAAcc                                                          <  8:282414/85‑1 (MQ=255)                                                                                                                                aaTGAGAAGGAGCTGTCTGATGATTAGACGAAGGAAACGCATGTTTACCGCTGTTACGTTGCTGGTCTTGTTGGTGATGGGAACCTCTGTATGTCCTGTGAAAGCTGAAGGGGCAGCGCGGCAGATGGa              <  2:55235/129‑1 (MQ=255)                                                                                                                                 aTGAGAAGGAGCTGTCTGATGATTAGACGAAGGAAACGCATGTTTACCGCTGTTACGTTg                                                                                  <  4:296692/60‑1 (MQ=255)                                                                                                                                 aTGAGAAGGAGCTGTCTGATGATTAGACGAAGGAAACGCATGTTTACCGCTGTTACGTTg                                                                                  >  3:296692/1‑60 (MQ=255)                                                                                                                                  tGAGAAGGAGCTGTCTGATGATTAGACGAAGGAAACGCATGTTTACCGCTGTTACGTTGCTGGTCTTGTTGGGGATGGGAACCTCTGTATGTCCTGTGAAAGCTGAAGGGGCAGCGCGGCAGATGGAAGCGCTGAACCg  <  6:137303/139‑1 (MQ=255)                                                                                                                                       aGGAGCTGTCTGATGATTAGACGAAGGAAACGCATGTTTAc                                                                                               <  6:233949/41‑1 (MQ=255)                                                                                                                                       aGGAGCTGTCTGATGATTAGACGAAGGAAACGCATGTTTAc                                                                                               >  5:233949/1‑41 (MQ=255)                                                                                                                                        |                                                                                                                                     CCGGGTAACAAAAAAATGACAAATAAGCAAAAAAGTGCAGTGTCCGAAATAGGCAGATGCAAAAAAGTGAAATTGAAGACAGCTCACAAAGCGCATAAAATCCAATTTAAGTAAGCGCTTACAGATAAATGAGAAGGAGCTGTCTGATGATTAGACGAAGGAAACGCATGTTTACCGCTGTTACGTTGCTGGTCTTGTTGGTGATGGGAACCTCTGTATGTCCTGTGAAAGCTGAAGGGGCAGCGCGGCAGATGGAAGCGCTGAACCG  >  BS168_reference_genome/770106‑770373

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑