| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | BS168_reference_genome | 892,022 | A→T | 100% | N196I (AAT→ATT) | malR → | transcriptional activator of the Mal operon |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | BS168_reference_genome | 892,022 | 0 | A | T | 100.0% | 178.1 / ‑6.6 | 52 | N196I (AAT→ATT) | malR | transcriptional activator of the Mal operon |
| Reads supporting (aligned to +/- strand): ref base A (0/1); new base T (21/30); total (21/31) | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
AATTTAATTTGATGCGCGATGATTTTAAAGTGAACGATCTGTTTATCATCATTTCTCTTTCCGGAGAAACACCGGAGCTGATTCCTCAGGCAAGAATGCTTTCTGCAAAGGGTATTCCCTTTATTTCCATTACGAATTTAAAAAACAATGTCCTCGCCCAGCTGACTCCGCAT‑AATCTTTATGCGACAAGCAAACCTGTGACATTAAGCGATAGAACGGAAATTGTCGCTTTTGCACCGTTTTTTCTCGTTGGCGAAGCATTGTTCCG > BS168_reference_genome/891887‑892154 | aatttaatttGATGCGCGATGATTTTAAAGTGAACGATCTGTTTATCATCATTTCTCTTTCCGGAGAAACACCGGAGCTGATTCCTCAGGCAAGAATGCTTTCTGCAAAGGGTATTCCCTTTATTTCCATTACGAttt > 8:284775/1‑138 (MQ=255) atgatTTTAAAGTGAACGATCTGTTTATCATCATTTCTCTTTCCGGAGAAACACCGGAGCTGATTCCTCAGGCAAGAATGCTTTCTGCAAAGGGTATTCCCTTTATTTCCATTACGATTTTAAAAAACAATGTCCTCGc < 5:308548/139‑1 (MQ=255) aaGTGAACGATCTGTTTATCATCATTTCTCTTTCCGGAGAAACACCGGAGCTGATTCCTCAGGCAAGAATGCTTTCTGCAAAGGGTATTCCCTTTATTTCCATTACGATTTTAAAAAACAATGTCCTCGCCCAGCTGAc < 8:74459/139‑1 (MQ=255) aaCGATCTGTTTATCATCATTTCTCTTTCCGGAGAAACACCGGAGCTGATTCCTCAGGCAAGAATGCTTTCTGCAAAGGGTATTCCCTTTATTTCCATTACGATTTTAAAAAACAATGTCCTCGCCCAGCTGACTCCg > 6:123905/1‑138 (MQ=255) aaCGATCTGTTTATCATCATTTCTCTTTCCGGAGAAACACCGGAGCTGATTCCTCAGGCAAGAATGCTTTCTGCAAAGGGTATTCCCTTTATTTCCATTACGATTTTAAAAAACAATGTCCTCGCCCAGCTGACTCCg > 4:198477/1‑138 (MQ=255) aaCGATCTGTTTATCATCATTTCTCTTTCCGGAGAAACACCGGAGCTGATTCCTCAGGCAAGAATGCTTTCTGCAAAGGGTATTCCCTTTATTTCCATTACGATTTTAAAAAACAATGTCCTCGCCCAGCTGACTCCg > 3:156872/1‑138 (MQ=255) gTTTATCATCATTTCTCTTTCCGGAGAAACACCGGAGCTGATTCCTCAGGCAAGAATGCTTTCTGCAAAGGGTATTCCCTTTATTTCCATTACGATTTTAAAAAACAATGTCCTCGCCCAGCTGACTCCGCAT‑AATCtt > 7:289672/1‑139 (MQ=255) tttATCTTCCTTTCTCTTGCCGGAGACACACCGGAGCTGATTACTCAGGCAAGAATGCTTTCTGCAAAGGGTATTCCCTTTATATCCATTACGATTTTAAAAAACAATGTCCTCGCCCAGCTGACTCCGCAT‑a < 4:246201/133‑1 (MQ=255) atcatTTCTCTTTCCGGAGAAACACCGGAGCTGATTCCTCAGGCAAGAATGCTTTCTGCAAAGTGTATTCCCTTTATTTCCATTACGATTTTAAAAAACAATGTCCTCGCCCAGCTGACTCCGCAT‑AATCTTTATGCg < 5:148393/138‑1 (MQ=255) atcatTTCTCTTTCCGGAGAAACACCGGAGCTGATTCCTCAGGCAAGAATGCTTTCTGCAAAGGGTATTCCCTTTATTTCCATTACGATTTTAAAAAACAATGTCCTCGCCCAGCTGACTCCGCAT‑AATCTTTATGCGa > 4:296013/1‑139 (MQ=255) tttCTCTTTCCTGAGAAACACCGGAGCTGATTCCTCAGGCAAGAATGCTTTCTGCAAAGGGTATTCCCTTTATTTCCATTACGATTTTAAAAAACAATGTCATCGCCCAGCTGACTCCGCAT‑AATCTTTATGCGAc > 2:137837/1‑136 (MQ=255) tttCTCTTTCCGGAGAAACACCGGAGCTGATTCCTCAGGCAAGAATGCTTTCTGCAAAGGGTATTCCCTTTATTTCCATTACGATTTTAAAAAACAATGTCCTCGCCCAGCTGACTCCGCAT‑AATCTTTATGCGACAAg < 1:237716/139‑1 (MQ=255) tttCTCTTTCCGGAGAAACACCGGAGCTGATTCCTCAGGCAAGAATGCTTTCTGCAAAGGGTATTCCCTTTATTTCCATTACGATTTTAAAAAACAATGTCCTCGCCCAGCTGACTCCGCAT‑AATCTTTATGCGACAAg > 4:305120/1‑139 (MQ=255) tttCTCTTTCCGGAGAAACACCGGAGCTGATTCCTCAGGCAAGAATGCTTTCTGCAAAGGGTATTCCCTTTATTTCCATTACGATTTTAAAAAACAATGTCCTCGCCCAGCTGACTCCGCAT‑AATCTTTATGCGACAAg < 4:196327/139‑1 (MQ=255) tttCTCTTTCCGGAGAAACACCGGAGCTGATTCCTCAGGCAAGAATGCTTTCTGCAAAGGGTATTCCCTTTATTTCCATTACGATTTTAAAAAACAATGTCATCGCCCAGCTGACTCCGCAT‑AATCTTTATGCGACAAg > 1:94885/1‑139 (MQ=255) tctcTTTCCGGAGAAACACCGGAGCTGATTCCTCAGGCAAGAATGCTTTCTGCAAAGGGTATTCCCTTTATTTCCATTACGATTTTAAAAAACAATGTCCTCGCCCAGCTGACTCCGCAT‑AATCTTTATGCGACAAGc < 1:168275/138‑1 (MQ=255) ccGGAGAAACACCGGAGCTGATTCCTCAGGCAAGAATGCTTTCTGCAAAGGGTATTCCCTTTATTTCCATTACGATTTTAAAAAACAATGTCCTCGCCCAGCTGACTCCGCAT‑AATCTTTATGCGACAAGCAAACCtg < 6:171676/138‑1 (MQ=255) cGGAGAAACACCGGAGCTGATTCCTCAGGCAAGAATGCTTTCTGCAAAGGGTATTCCCTTTATTTCCATTACGATTTTAAAAAACAATGTCCTCGCCCAGCTGACTCCGCAT‑AATCTTTATGCGACAAGCAAACCtgt < 4:259128/138‑1 (MQ=255) agaAACACCGGAGCTGATTCCTCAGGCAAGAATGCTTTCTGCAAAGGGTATTCCCTTTATTTCCATTACGATTTTAAAAATCAATGTCCTCGCCCAGCTGACTCCGCAT‑AATCTTTATGCGACAAGCAAACCTGTGACa < 4:31380/139‑1 (MQ=255) cGGAGCTGATTCCTCAGGCAAGAATGCTTTCTGCAAAGGGTATTCCCTTTATTTCCATTACGATTTTAAAAAACAATGTCCTCGCCCAGCTGACTCCGCAT‑AATCTTTATGCGACAAGCAAACCTGTGACATTAAGCGa < 3:182886/139‑1 (MQ=255) cGGAGCTGATTCCTCAGGCAAGAATGCTTTCTGCAAAGGGTATTCCCTTTATTTCCATTACGATTTTAAAAAACAATGTCCTCGCCCAGCTGACTCCGCAT‑AATCTTTATGCGACAAGCAAACCTGTGACATTAAGCGa < 7:284775/139‑1 (MQ=255) ggAGCTGATTCCTCAGGCAAGAATGCTTTCTGCAAAGGGTATTCCCTTTATTTCCATTACGATTTTAAAAAACAATGTCCTCGCCCAGCTGACTCCGCAT‑AATCTTTATGCGACAAGCAAACCTGTGACATTAAGCGa > 2:204136/1‑138 (MQ=255) aTTCCTCAGGCAAGAATGCTTTCTGCAAAGGGTATTCACTTTATTTCCATTACGATTTTAAAAAACAATGTCCTCGCCCAGCTGACTCCGCAT‑AATCTTTATGCGACAAGCAAACCTCAGACATTAAGAGATAGAACg > 4:182886/1‑138 (MQ=255) aGGCAAGAATGCTTTCTGCAAAGGGTATTCCCTTTATTTCCATTACGATTTTAAAAAACAATGTCCTCGCCCAGCTGACTCCGCAT‑AATCTTTATGCGACAAGCAAACCTGTGACATTAAGCGATAGAACGGAAATTGt < 1:204136/139‑1 (MQ=255) aGGCAAGAATGCTTTCTGCAAAGGGTATTCCCTTTATTTCCATTACGATTTTAAAAAACAATGTCCTCGCCCAGCTGACTCCGCAT‑AATCTTTATGCGACAAGCAAACCTGTGACATTAAGCGATAGAACGGAAATTGt < 2:15247/139‑1 (MQ=255) cTTTCTGCAAAGGGTATTCCCTTTATTTCCATTACGATTTTAAAAAACAATGTCCTCGCCCAGCTGACTCCGCAT‑AATCTTTATGCGACAAGCAAACCTGTGACATTAAGCGATAGAACGGAAATTGTCGCTTTTGCAc < 8:289672/139‑1 (MQ=255) cTTTCTGCAAAGGGTATTCCCTTTATTTCCATTACGATTTTAAAAAACAATGTCCTCGCCCAGCTGACTCCGCAT‑AATCTTTATGCGACAAGCAAACCTGTGACATTAAGCGATAGAACGGAAATTGTCGCTTTTGCAc < 3:296013/139‑1 (MQ=255) tttCTGCAAAGGGTATTCCCTTTATTTCCATTACGATTTTAAAAAACAATGTCCTCGCCCAGCTGACTCCGCAT‑AATCTTTATGCGACAAGCAAACCTGTGACATTAAGCGATAGAACGGAAATTGTCGCTTTTGCAcc > 6:20407/1‑139 (MQ=255) tttCTGCAAAGGGTATTCCCTTTATTTCCATTACGATTTTAAAAAACAATGTCCTCGCCCAGCTGACTCCGCAT‑AATCTTTATGCGACAAGCAAACCTGTGACATTAAGCGATAGAACGGAAATTGTCGCTTTTGCAcc > 2:151350/1‑139 (MQ=255) tttCTGCAAAGGGTATTCCCTTTATTTCCATTACGATTTTAAAAAACAATGTCCTCGCCCAGCTGACTCCGCAT‑AATCTTTATGCGACAAGCAAACCTGTGACATTAAGCGATAGAACGGAAATTGTCGATTTTGCTcc > 2:13009/1‑139 (MQ=255) gCAAAGGGTATTCCCTTTATTTCCATTACGATTTTAAAAAACAATGTCCTCGCCCAGCTGACTCCGCAT‑AATCTTTATGCGACAAGCAAACCTGTGACATTAAGCGATAGAACGGAAATTGTCGCTTTTGCACCGtttt > 6:196129/1‑139 (MQ=255) aaaGGGTATTCCCTTTATTTCCATTACGATTTTAAAAAACAATGTCCTCGCCCAGCTGACTCCGCAT‑AATCTTTATGCGACAAGCAAACCTGTGACATTAAGCGATAGAACGGAAATTGTCGCTTTTGCACCGtttttt < 2:22097/139‑1 (MQ=255) aGGGTATTCCCTTTATTTCCATTACGATTTTAAAAAACAATGTCCTCGCCCAGCTGACTCCGCAT‑AATCTTTATGCGACAAGCa < 7:256059/84‑1 (MQ=255) aGGGTATTCCCTTTATTTCCATTACGATTTTAAAAAACAATGTCCTCGCCCAGCTGACTCCGCAT‑AATCTTTATGCGACAAGCa > 8:256059/1‑84 (MQ=255) aTTCCCTTTATTTCCATTACGATTTTAAAAAACAATGTCCTCGCCCAGCTGACTCCGCAT‑AATCTTTATGCGACAAGCAAACCTGTGACATTAAGCGATAGAACGGAAATTGTCGCTTTTGCACCGTTTTTTCTCGTTg > 7:99094/1‑139 (MQ=255) ccTTTATTTCCATTACGATTTTAAAAAACAATGTCCTCGCCCAGCTGACTCCGCAT‑AATCTTTATGCGACAAGCAAACCTGTGACATTAAGCGATAGAACGGAAATTGTCGCTTTTGCACCGTTTTTTCTCGTTGGCg < 7:62743/138‑1 (MQ=255) ccTTTATTTCCATTACGATTTTAAAAAACAATGTCCTCGCCCAGCTGACTCCGCAT‑AATCTTTATGCGACAAGCAAACCTGTGACATTAAGCGATAGAACGGAAATTGTCGCTTTTGCACCGTTTTTTCTCGTTGGCg < 1:137837/138‑1 (MQ=255) ccTTTATTTCCATTACGATTTTAAAAAACAATGTCCTCGCCCAGCTGACTCCGCAT‑AATCTTTATGCGACAAGCAAACCTGTGACATTAAGCGATAGAACGGAAATTGTCGCTTTTGCACCGTTTTTTCTCGTTGGCg < 4:120117/138‑1 (MQ=255) ccTTTATTTCCATTACGATTTTAAAAAACAATGTCCTCGCCCAGCTGACTCCGCAT‑AATCTTTATGCGACAAGCAAACCTGTGACATTAAGCGATAGAACGGAAATTGTCGCTTTTGCACCGTTTTTTCTCGTTGGCg < 3:305120/138‑1 (MQ=255) ccTTTATTTCCATTACGATTTTAAAAAACAATGTCCTCGCCCAGCTGACTCCGCAT‑AATCTTTATGCGACAAGCAAACCTGTGACATTAAGCGATAGAACGGAAATTGTCGCTTTTGCACCGTTTTTTCTCGTTGGCg < 3:198477/138‑1 (MQ=255) ccTTTATTTCCATTACGATTTTAAAAAACAATGTCCTCGCCCAGCTGACTCCGCAT‑AATCTTTATGCGACAAGCAAACCTGTGACATTAAGCGATAGAACGGAAATTGTCGCTTTTGCACCGTTTTTTCTCGTTGGCg < 3:131786/138‑1 (MQ=255) ttATTTCCATTACGATTTTAAAAAACAATGTCCTCGCCCAGCTGACTCCGCAT‑AATCTTTATGCGACAAGCAAACCTGTGACATTAAGCGATAGAACGGAAATTGTCGCTTTTGCACCGTTTTTTCTCGTTGGCg < 2:180067/135‑1 (MQ=255) ttATTTCCATTACGATTTTAAAAAACAATGTCCTCGCCCAGCTGACTCCGCAT‑AATCTTTATGCGACAAGCAAACCTGTGACATTAAGCGATAGAACGGAAATTGTCGCTTTTGCACCGTTTTTTCTCGTTGGCg > 1:180067/1‑135 (MQ=255) ttATTTCCATTACGATTTTAAAAAACAATGTCCTCGCCCAGCTGACTCCGCAT‑AATCTTTATGCGACAAGCAAACCTGTGACATTAAGCGATAGAACGGAAATTGTCGCTTTTGCACCGTTTTTTCTCGTTGGCGAAg > 1:188994/1‑138 (MQ=255) ttATTTCCATTACGATTTTAAAAAACAATGTCCTCGCCCAGCTGACTCCGCAT‑AATCTTTATGCGACAAGCAAACCTGTGACATTAAGCGATAGAACGGAAATTGTCGCTTTTGCACCGATTTTTCTCGTTTGCGAAg > 4:181570/1‑138 (MQ=255) tATTTCCATTACGATTTTAAAAAACAATGTCCTCGCCCAGCTGACTCCGCa > 8:83681/1‑51 (MQ=255) tATTTCCATTACGATTTTAAAAAACAATGTCCTCGCCCAGCTGACTCCGCa < 7:83681/51‑1 (MQ=255) attCCATTACGAATTTTAAAAACAAAGTCCTCGCCCACCGGTCTCCGCATAAACCTTAAAGCGACAAGCAAACCTGTGACATTAAGCGATAGAACGGAAATTGTCGCTTTTGCACCGTTTTTTCTCGTTGGCGAAGCa < 2:94885/137‑1 (MQ=255) cATTACGATTTTAAAAAACAATGTCCTCGCCCAGCTGACTCCGCAT‑AATCTTTATGCGACAAGCAAACCTGTGACATTAAGCGATAGAACGGAAATTGt < 5:43635/99‑1 (MQ=255) cATTACGATTTTAAAAAACAATGTCCTCGCCCAGCTGACTCCGCAT‑AATCTTTATGCGACAAGCAAACCTGTGACATTAAGCGATAGAACGGAAATTGt > 6:43635/1‑99 (MQ=255) cATTACGATTTTAAAAAACAATGTCCTCGCCCAGCTGACTCCGCAT‑AATCTTTATGCGACAAGCAAACCTGTGACATTAAGCGATAGAACGGAAATTGTCGCTTTTGCACCGTTTTTTCTCGTTGGCGAAGCATTGTTc < 8:99094/139‑1 (MQ=255) cATTACGATTTTAAAAAACAATGTCCTCGCCCAGCTGACTCCGCAT‑AATCTTTATGCGACAAGCAAACCTGTGACATTAAGCGATAGAACGGAAATTGTCGCTTTTGCACCGTTTTTTCTCGTTGGCGAAGCATTGTTc < 2:98514/139‑1 (MQ=255) ttACGATTTTAAAAAACAATGTCCTCGCCCAGCTGACTCCGCAT‑AATCTTTATGCGACAAGCAAACCTGTGACATTAAGCGATAGAACGGAAATTGTCGCTTTTGCACCGTTTTTTCTCGTTGGCGAAGCATTGTTCcg < 5:123905/139‑1 (MQ=255) | AATTTAATTTGATGCGCGATGATTTTAAAGTGAACGATCTGTTTATCATCATTTCTCTTTCCGGAGAAACACCGGAGCTGATTCCTCAGGCAAGAATGCTTTCTGCAAAGGGTATTCCCTTTATTTCCATTACGAATTTAAAAAACAATGTCCTCGCCCAGCTGACTCCGCAT‑AATCTTTATGCGACAAGCAAACCTGTGACATTAAGCGATAGAACGGAAATTGTCGCTTTTGCACCGTTTTTTCTCGTTGGCGAAGCATTGTTCCG > BS168_reference_genome/891887‑892154 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |