Predicted mutation
evidence seq id position mutation freq annotation gene description
RA BS168_reference_genome 1,012,003 G→T 7.3% A419E (GCA→GAA)  lytF ← gamma‑D‑glutamate‑meso‑diaminopimelate muropeptidase (major autolysin, vegetative)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*BS168_reference_genome1,012,0030GT 7.3% 182.1 / 4.5 55A419E (GCA→GAA) lytFgamma‑D‑glutamate‑meso‑diaminopimelate muropeptidase (major autolysin, vegetative)
Reads supporting (aligned to +/- strand):  ref base G (22/29);  new base T (2/2);  total (24/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.72e-01

TTGGCATTTATGAATTCCCCGTTGCCTAGGTAAATTCCGACGTGGGAAGGGCCGGCTTTATATGTTGAAAAGAAAACGAAATCTCCGACAGCAGGCTGGCTAACTGATTTCATTGTGTTCCAATATCCCGCTGCCGTTAATCTAGATACGGATGTGACTTTGTTCAGTACATAGTAAATGAATCCGCTGCAGTCAAACCCGGACGGTGTCGTGCCTCCCCAGCGATACGGAACCCCAAGCTGCGCTTTAGCGGCCGAAATCATTGTGT  >  BS168_reference_genome/1011871‑1012138
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ttGGCATTTATGAATTCCCCGTTGCCTAGGTAAATTCCGACGTGGGAAGGGCCGGCTTTATATGTTGAAAAGAAAACGAAATCTCCGACAGCAGGCTGGCTAACTGATTTCATTGTGTTCCAATATCCCGCTGCCGtt                                                                                                                                    >  2:85640/1‑138 (MQ=255)
      tttATGTCTTCCCCGTTGCCTATGTAAATTCCGACGTGGGAAGGGCCGGCTTTATATGTTGAAAAGAAAACGAAATCTCCGACAGCAGGCTGGCTAACTGATTTCATTGTGTTCCAATATCCCGCTGCCGTTAATCTAg                                                                                                                             <  6:186703/139‑1 (MQ=255)
         aTGAATTCCCCGTTGCCTAGGTAAATTCCGACGTGGGAAGGGCCGGCTTTATATGTTGAAAAGAAAACGAAATCTCCGACAGCAGGCTGGCTAACTGATTTCATTGTGTTCCAATATCCCGCTGCCGTTAATCTAGAt                                                                                                                           <  4:57585/138‑1 (MQ=255)
               tCCCCGTTGCCTAGGTAAATTCCGACGTGGGAAGGGCCGGCTTTATATGTTGAAAAGAAAACGAAATCTCCGACAGCAGGCTGGCTAACTGATTTCATTGTGTTCCAATATCCCGCTGCCGTTAATCTAGATACGGAtg                                                                                                                    >  6:190742/1‑139 (MQ=255)
                    gTTGCCTAGGTAAATTCCGACGTGGGAAGGGCCGGCTTTATATGTTGAAAAGAAAACGAAATCTCCGACAGCAGGCTGGCTAACTGATTTCATTGTGTTCCAATATCCCGCTGCCGTTAATCTAGATACGGATGTGACt                                                                                                               <  1:126921/139‑1 (MQ=255)
                    gTTGCCTAGGTAAATTCCGACGTGGGAAGGGCCGGCTTTATATGTTGAAAAGAAAACGAAATCTCCGACAGCAGGCTGGCTAACTGATTTCATTGTGTTCCAATATCCCGCTGCCGTTAATCTAGATACGGATGTGACt                                                                                                               <  5:259355/139‑1 (MQ=255)
                    gTTGCCTAGGTAAATTCCGACGTGGGAAGGGCCGGCTTTATATGTTGAAAAGAAAACGAAATCTCCGACAGCAGGCTGGCTAACTGATTTCATTGTGTTCCAATATCCCGCTGCCGTTAATCTAGATACGGATGTGACt                                                                                                               <  2:19599/139‑1 (MQ=255)
                         cTATGTAAATTCCGACGTGGGAAGGGCCGGCTTTATATGTTGAAAAGAAAACGAAATCTCCGACAGCAGGCTGGCTAACTGATTTCATTGTGTTCCAATATCCCGCTGCCGTTAATCTAGATACGGATGTGACTTTGtt                                                                                                          <  2:176367/139‑1 (MQ=255)
                         cTAGGTAAATTCCGACGTGGGAAGGGCCGGCTTTATATGTTGAAAAGAAAACGAAATCTCCGACAGCAGGCTGGCTAACTGATTTCATTGTGTTCCAATATCCCGCTGCCGTTAATCTAGATACGGATGTGACTTTGtt                                                                                                          <  1:85640/139‑1 (MQ=255)
                         cTAGGTAAATTCCGACGTGGGAAGGGCCGGCTTTATATGTTGAAAAGAAAACGAAATCTCCGACAGCAGGCTGGCTAACTGATTTCATTGTGTTCCAATATCCCGCTGCCGTTAATCTAGATACGGATGTGACTTTGtt                                                                                                          <  6:128462/139‑1 (MQ=255)
                         cTAGGTAAATTCCGACGTGGGAAGGGCCGGCTTTATATGTTGAAAAGAAAACGAAATCTCCGACAGCAGGCTGGCTAACTGATTTCATTGTGTTCCAATATCCCGCTGCCGTTAATCTAGATACGGATGTGACTTTGtt                                                                                                          <  2:109270/139‑1 (MQ=255)
                           aGGTAAATTCCGACGTGGGAAGGGCCGGCTTTATATGTTGAAAAGAAAACGAAATCTCCGACAGCAGGCTGGCTAACTGATTTCATTGTGTTCCAATATCCCGCTGCCGTTAATCTAGATACGGATGTGACTTTGtt                                                                                                          >  1:62188/1‑137 (MQ=255)
                           aGGTAAATTCCGACGTGGGAAGGGCCGGCTTTATATGTTGAAAAGAAAACGAAATCTCCGACAGCAGGCTGGCTAACTGATTTCATTGTGTTCCAATATCCCGCTGCCGTTAATCTAGATACGGATGTGACTTTGtt                                                                                                          >  1:242220/1‑137 (MQ=255)
                              tAAATTCCGACGTGGGAAGGGCCGGCTTTATATGTTGAAAAGAAAACGAAATCTCCGACAGCAGGCTGGCTAACTGATTTCATTGTGTTCCAATATCCCGCTGCCGTTAATCTAGATACGGATGTGACTTTGTTCAGTa                                                                                                     <  5:190742/139‑1 (MQ=255)
                              tAAATTCCGACGTGGGAAGGGCCGGCTTTATATGTTGAAAAGAAAACGAAATCTCCGACAGCAGGCTGGCTAACTGATTTCATTGTGTTCCAATATCCCGCTGCCGTTAATCTAGATACGGATGTGACTTTGTTCAGTa                                                                                                     <  3:238713/139‑1 (MQ=255)
                                aaatCCGACGTGGGAAGGGCCGGCTTTATATGTTGAAAAGAAAACGAAATCTCCGACAGCAGGCTGGCTAACTGATTTCATTGTGTTCCAATATCCCGCTGCCGTTAATCTAGATACGGATGTGACTTTGTTCAGTACa                                                                                                   <  4:124231/136‑1 (MQ=255)
                                aaTTCCGACGTGGGAAGGGCCGGCTTTATATGTTGAAAAGAAAACGAAATCTCCGAGAGCAGGCTGGCTAACTGATTTCATTGTGTTCCAATATCCCGCTGCCGTTAATCTAGATACGGATGTGACTTTGTTCAGTACa                                                                                                   <  6:290543/139‑1 (MQ=255)
                                  ttCCGACGTGGGAAGGGCCGGCTTTATATGTTGAAAAGAAAACGAAATCTCCGACAGCAGGCTGGCTAACTGATTTCATTGTGTTCCAATATCCCGCTGCCGTTAATCTAGATACGGATGTGACTTTGTTCAGTACATa                                                                                                 <  2:242220/139‑1 (MQ=255)
                                  ttCCGACGTGGGAAGGGCCGGCTTTATATGTTGAAAAGAAAACGAAATCTCCGACAGCAGGCTGGCTAACTGATTTCATTGTGTTCCAATATCCCGCTGCCGTTAATCTAGATACGGATGTGACTTTGTTCAGTACATa                                                                                                 <  1:188127/139‑1 (MQ=255)
                                       aCGTGGGAAGGGCCGGCTTTATATGTTGAAAAGAAAACGAAATCTCCGACAGCAGGCTGGCTAACTGATTTCATTGTGTTCCAATATCCCGCTGCCGTTAATCTAGATACGGATGTGACt                                                                                                               <  5:146378/120‑1 (MQ=255)
                                       aCGTGGGAAGGGCCGGCTTTATATGTTGAAAAGAAAACGAAATCTCCGACAGCAGGCTGGCTAACTGATTTCATTGTGTTCCAATATCCCGCTGCCGTTAATCTAGATACGGATGTGACt                                                                                                               >  6:146378/1‑120 (MQ=255)
                                                      gCTTTATATGTTGAAAAGAAAACGAAATCTCCGACAGCAGGCTGGCTAACTGATTTCATTGTGTTCCAATATACCGCTGCCGTTAATCTAGATACGGATGTGACTTTGTTCAGTACATAGTAAATGAATCCGCTGCAGt                                                                             <  2:62188/139‑1 (MQ=255)
                                                         ttATATGTTGAAAAGAAAACGAAATCTCCGACAGCTGGCTGGCTAACTGATTTCATTGTGTTCCAATATCCCGCTGCCGTTAATCTAGATACGGATGTGACTTTTTTAAGTACATAGTAAATGAATCCGCTCCAGTCa                                                                           <  7:71341/138‑1 (MQ=255)
                                                           atatGTTGAAAAGAAAACGAAATCTCCGACAGCAGGCTGGCTAACTGATTTCATTGTGTTCCAATATCCCGCTTCCGTTAATCTAGATACGGATGTGACTTTGTTCAGTa                                                                                                     <  3:276461/110‑1 (MQ=255)
                                                           atatGTTGAAAAGAAAACGAAATCTCCGACAGCAGGCTGGCTAACTGATTTCATTGTGTTCCAATATCCCGCTTCCGTTAATCTAGATACGGATGTGACTTTGTTCAGTa                                                                                                     <  3:280960/110‑1 (MQ=255)
                                                           atatGTTGAAAAGAAAACGAAATCTCCGACAGCAGGCTGGCTAACTGATTTCATTGTGTTCCAATATCCCGCTTCCGTTAATCTAGATACGGATGTGACTTTGTTCAGTa                                                                                                     >  4:280960/1‑110 (MQ=255)
                                                           atatGTTGAAAAGAAAACGAAATCTCCGACAGCAGGCTGGCTAACTGATTTCATTGTGTTCCAATATCCCGCTTCCGTTAATCTAGATACGGATGTGACTTTGTTCAGTa                                                                                                     >  4:276461/1‑110 (MQ=255)
                                                                ttGAAAAGAAAACGAAATCTCCGACAGCAGGCTGGCTAACTGATTTCATTGTGTTCCAATATCCCGCTGCCGTTTATCTAGATACGGATGTGACTTTGTTCAGTACATAGTAAATGAAGCCGCTGCAGTCAAACCCGGa                                                                   >  7:249507/1‑139 (MQ=255)
                                                                 tGAAAAGAAAACGAAATCTCCGACAGCAGGCTGGCTAACTGATTTCATTGTGTTCCAATATCCCGCTGCCGtt                                                                                                                                    <  1:112223/73‑1 (MQ=255)
                                                                 tGAAAAGAAAACGAAATCTCCGACAGCAGGCTGGCTAACTGATTTCATTGTGTTCCAATATCCCGCTGCCGtt                                                                                                                                    >  2:112223/1‑73 (MQ=255)
                                                                 tGAAAAGAAAACGAAATCTCCGACAGCAGGCTGGCTAACTGATTTCATTGTGTTCCAATATCCCGCTGCCGTTAATCt                                                                                                                               >  2:130771/1‑78 (MQ=255)
                                                                 tGAAAAGAAAACGAAATCTCCGACAGCAGGCTGGCTAACTGATTTCATTGTGTTCCAATATCCCGCTGCCGTTAATCt                                                                                                                               <  1:130771/78‑1 (MQ=255)
                                                                 tGAAAAGAAAACGAAATCTCCGACAGCAGGCTGGCTAACTGATTTCATTGTGTTCCAATATCCCGCTGCCGTTAATCTAGATACGGATGTGACTTTGTTCAGTACa                                                                                                   <  6:297880/106‑1 (MQ=255)
                                                                 tGAAAAGAAAACGAAATCTCCGACAGCAGGCTGGCTAACTGATTTCATTGTGTTCCAATATCCCGCTGCCGTTAATCTAGATACGGATGTGACTTTGTTCAGTACa                                                                                                   >  5:297880/1‑106 (MQ=255)
                                                                 tGAAAAGAAAACGAAATCTCCGACAGCAGGCTGGCTAACTGATTTCATTGTGTTCCAATATCCCGCTGCCGTTAATCTAGATACGGATGTGACTTTGTTCAGTACATAGTAAATGAATCCGCTGCAGTCAACCCCGGAc                                                                  >  2:311265/1‑139 (MQ=255)
                                                                 tGAAAAGAAAACGAAATCTCCGACAGCAGGCTGGCTAACTGATTTCATTGTGTTCCAATATCCCGCTGCCGTTAATCTAGATACGGATGTGACTTTGTTCAGTACATAGTAAATGAATCCGCTGCAGTCAAACCCGGAc                                                                  >  3:282615/1‑139 (MQ=255)
                                                                 tGAAAAGAAAACGAAATCTCCGACAGCAGGCTGGCTAACTGATTTCATTGTGTTCCAATATCCCGCTGCCGTTAATCTAGATACGGATGTGACTTTGTTCAGTACATAGTAAATGAATCCGCTGCAGTCAAACCCGGAc                                                                  >  1:142198/1‑139 (MQ=255)
                                                                                aTCTCCGACAGCAGGCTGGCTAACTGATTTCATTGTGTTCCAATATCCCGCTGCCGTTAATCTAGATACGGATGTGACTTTGTTCAGTACATAGTAAATGAATCCGCTGCAGTCAAACCCGGACGGTGTCGTGCCTccc                                                   <  2:142198/139‑1 (MQ=255)
                                                                                           cagGCTGGCTAACTGATTTCATTGTGTTCCAATATCCCGCTGCCGTTAATCTAGATa                                                                                                                          >  2:88798/1‑57 (MQ=255)
                                                                                           cagGCTGGCTAACTGATTTCATTGTGTTCCAATATCCCGCTGCCGTTAATCTAGATa                                                                                                                          <  1:120031/57‑1 (MQ=255)
                                                                                           cagGCTGGCTAACTGATTTCATTGTGTTCCAATATCCCGCTGCCGTTAATCTAGATa                                                                                                                          <  1:88798/57‑1 (MQ=255)
                                                                                           cagGCTGGCTAACTGATTTCATTGTGTTCCAATATCCCGCTGCCGTTAATCTAGATa                                                                                                                          >  2:120031/1‑57 (MQ=255)
                                                                                                     aaCTGATTTCATTGTGTTCCAATATCCCGCTGCCGTTAATCTAGATACGGATGTGACTTTGTTCAGTACATAGTAAATGAATCCGCTGCAGTCAAACCCGGACGGTGTCGTGCCTCCCCAGCGATACGGAACCCCAAGc                              >  3:122256/1‑139 (MQ=255)
                                                                                                               aTTGTGCTCCAATATCCCGCTGCCGTTAATCTAGATACGGATGTGACTTTGTTCAGTACATAGTAACTGAATCCGCTGCAGTCAAACCCGGACGGGGTCGTGCCTCCCCAGCGATACGGAACCCCAAGCTGCGCTTTAg                    <  5:230948/139‑1 (MQ=255)
                                                                                                                     ttCCAATATCCCGCTGCCGTTAATCTAGATACGGATGTGACTTTGtt                                                                                                          >  5:13013/1‑47 (MQ=255)
                                                                                                                     ttCCAATATCCCGCTGCCGTTAATCTAGATACGGATGTGACTTTGtt                                                                                                          <  6:13013/47‑1 (MQ=255)
                                                                                                                     ttCCAATATCCCGCTGCCGTTAATCTAGATACGGATGTGACTTTGTTCAGTa                                                                                                     >  2:40830/1‑52 (MQ=255)
                                                                                                                     ttCCAATATCCCGCTGCCGTTAATCTAGATACGGATGTGACTTTGTTCAGTa                                                                                                     <  1:40830/52‑1 (MQ=255)
                                                                                                                           tatCCCGCTGCCGTTAATCTAGATACGGATGTGACTTTGTTCAGTACATAGTAAATGAATCCGCTGCAGTCAAACCCGGACGGTGTCGTGCCTCCCCAGCGATACGGAACCCCAAGCTGCGCTTTAGCGGCCGAAATCa        <  5:77050/139‑1 (MQ=255)
                                                                                                                               ccGCTGCCGTTAATCTAGATACGGATGTGACTTTGTTCAGTACATAGTAAATGAATCCGCTGCAGTCAAACCCGGACGGTGTCGTGCCTCCCCAGCGATACGGAACCCCAAGCTGCGCTTTAGCGGCCGAAATCATtgt    >  5:111326/1‑139 (MQ=255)
                                                                                                                                  cTGCCGTTAATCTAGATACGGATGTGACTTTGTTCAGTa                                                                                                     >  4:51033/1‑39 (MQ=255)
                                                                                                                                  cTGCCGTTAATCTAGATACGGATGTGACTTTGTTCAGTa                                                                                                     <  3:51033/39‑1 (MQ=255)
                                                                                                                                  cTGCCGTTAATCTAGATACGGATGTGACTTTGTTCAGTACATAGTAAATGAATCCGCTGCAGTCAAACCCGGACGGTGTCGTGCCTCCCCAGCGATACGGAACCCCAAGCTGCGCTTTAGCGGCCGAAATCATTgtgt  >  5:95937/1‑138 (MQ=255)
                                                                                                                                  cTGCCGTTAATCTAGATACGGATGTGACTTTGTTCAGTACATAGTAAATGAATCCGCTGCAGTCAAACCCGGACGGTGTCGTGCCTCCCCAGCGATACGGAACCCCAAGCTGCGCTTTAGCGGCCGAAATCATTgtgt  >  3:226128/1‑138 (MQ=255)
                                                                                                                                  cTGCCGTTAATCTAGATACGGATGTGACTTTGTTCAGTACATAGTAAATGAATCCGCTGCAGTCAAACCCGGACGGTGTCGTGCCTCCCCAGCGATACGGAACCCCAAGCTGCGCTTTAGCGGCCGAAATCATTgtgt  >  2:188521/1‑138 (MQ=255)
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TTGGCATTTATGAATTCCCCGTTGCCTAGGTAAATTCCGACGTGGGAAGGGCCGGCTTTATATGTTGAAAAGAAAACGAAATCTCCGACAGCAGGCTGGCTAACTGATTTCATTGTGTTCCAATATCCCGCTGCCGTTAATCTAGATACGGATGTGACTTTGTTCAGTACATAGTAAATGAATCCGCTGCAGTCAAACCCGGACGGTGTCGTGCCTCCCCAGCGATACGGAACCCCAAGCTGCGCTTTAGCGGCCGAAATCATTGTGT  >  BS168_reference_genome/1011871‑1012138

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑