| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | BS168_reference_genome | 1,023,150 | C→A | 34.8% | intergenic (+50/‑200) | yhdF → / → bcaP | putative NAD(P)‑dependent dehydrogenase/branched‑chain amino acid transporter or methylthioribose‑degradation metabolite exporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | BS168_reference_genome | 1,023,150 | 0 | C | A | 34.8% | 82.0 / 8.0 | 51 | intergenic (+50/‑200) | yhdF/bcaP | putative NAD(P)‑dependent dehydrogenase/branched‑chain amino acid transporter or methylthioribose‑degradation metabolite exporter |
| Reads supporting (aligned to +/- strand): ref base C (9/21); new base A (10/6); total (24/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.81e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TGCTGGCGTCTGACGAATCTTCCTATATGACAGGGCAGACCATTCATGTGAATGGCGGCCGTTTTATTTCAACGTAATCAACGAAAAACCAGCTCAAGAGCTGGT‑TTTTTG‑TGTGGTG‑AAAAATTTCAAAATAAAAAATTTGT‑CGATTTTTCTAACAATTTTGTTTTCATTTTTGCAATTTTATATATAATATTGAGTAGGAGAAAGGAATGATTCGCGACCAGTACAAATATAGTAGAAAAAAAAGAATAATGAC > BS168_reference_genome/1023024‑1023279 | tgctgGCGTCTGACGAATCTTCCTATATGACAGGGCAGACCATTCATGTGAATGGCGGCCGGTTTATTTTAAAGTAATAAACGAAAAAACCCCGAAAGAGCGGGT‑TTTTTT‑TGTGGTG‑GAAAATTTGAAAAAAAAAAAt > 7:174690/1‑139 (MQ=255)tgctgGCGTCTGACGAATCATCCTATATGACAGGGCAGACCATTCATGTGAATGGCGGCCGTTTTTTTTTAACGTTATGAACGAAAAACCAACACAAGTGCTGGG‑GTTTTG‑TTTGGTG‑GAAAATTTGAAAATAAAAAAt > 6:157662/1‑139 (MQ=255) ctgGCGTCTGACGAATCTTCCTATATGACAGGGCAGACCATTCATGTGAATGGCGGCCGTTTTTTTTTAACGTAATAAACCAAAAAAAAGCTCAATAGCGGGT‑TTTTTT‑TTTTGTT‑TTAAAATTAAAAATAAAAAAatt > 3:103233/1‑139 (MQ=255) ctgGCGTCTGACGAATCTTCCTATATGACAGGGCAGACCATTCATGTGAATGGCGGCCGTGTTTTTTTAACCTAAACAAACAAAAAACACCACAAAACGGGGG‑GTTTTG‑TGTGGGGTTAAAATTTAAAAAAAAAAAAta > 7:218339/1‑138 (MQ=255) aaTCTTCCTATATGACAGGGCAGACCATTCATGTGAATGGCGGCCGTTTTTTTTAAACGTAATCAACGAAAAAACACCTCAAGAGCGGGG‑TTTTTG‑TGTGGGG‑TAAAATTTAAAAATAAAAAAATTTT‑GGAttttttt > 8:61132/1‑136 (MQ=255) tCTTCCTATATGACAGGGCAGACCATTCATGTGAATGGCGGCCGTGTTTTTTGAACGGAATAAACCAAAAACCACCACAACATCTGGT‑GTTTTT‑TGTGGTG‑TTAAATTTAAAAATAAAAAAATTTT‑AGAtttttttta > 4:182144/1‑134 (MQ=255) aGGGCAGACCATTCATGTGAATGGCGGCCGTTTTATTTAAACGTAATAAAAGAAAAAACACCACAAAAGCTGGG‑TTTTTG‑TGTGGTG‑TAAAATTTAAAAAAAAAAAAATTTT‑CGATTTTTGTAAAAATTTTTTTTTTAt > 7:227034/1‑139 (MQ=255) ggCAGACCATTCATGTGAATGGCGGCCGTTTTTTTTTAAAGTTATAAAACAAAAACCAACACAAAAGCAGGG‑TTTTTG‑TGTGGTG‑AAAAAATTAAAAAAAAAAAAATTTT‑ACATTTTTTTAAAAAAttttttttaaatt > 6:62474/1‑134 (MQ=255) ggCAGACCATTCATGTGAATGGCGGCCGTTTTTTTTAAACGTAATAAACGAAAAAACACCTCAAGCGCTGGT‑TTTTTT‑TGTGGTG‑AAAAATTTAAAAATAAAAAATTTTT‑GGATTTTTATAAAAATTTTTTTTTGAttt > 5:96073/1‑139 (MQ=255) ggCAGACCATTCATGTGAATGGCGGCCGTTTTATTTCAAAGTAATAAACCAAAAAACCGCTCAAGAGCGGGG‑TTTTTG‑TGTGGTG‑TAAAATTTTAAAATAAAAAATTTTT‑GGGTTTTTGTAACAATttttttttgtttt > 7:150898/1‑134 (MQ=255) ggCAGACCATTCATGTGAATGGCGGCCGTGTTTTTTTAAAGTTATAAAAAAAAAAACACCACAAAAGCAGGT‑TTTTTGTTGTGGT‑‑TAAAATTTTAAAAAAAAAAATTTTT‑TGTTTTTTTTAAAAAATTTTTTTTTAttt > 4:54265/1‑139 (MQ=255) ggCAGACCATTCATGTGAATGGCGGCCGTGTTTTTTAAAAATAATAAAACAAAAAACACCACAAGAGCAGGG‑TTTTTT‑TTTTGTG‑TAAAATTTAAAAAAAAAAAAATTTT‑AGATTTTTATTAAAAATTTTTTTTTAttt > 4:188081/1‑139 (MQ=255) cAGACCATTCATGTGAATGGCGGCCGTTTTTTTTGAACGTATTAAACGAAAAAACACCGCAAGACGGGTT‑TTTTTG‑TTTGGTG‑AAAAATTTTAAAATAAAAAAATATT‑GGATTTTTTTTACAAATTTTTTTTGAttttt > 6:251464/1‑139 (MQ=255) aGACCATTCATGTGAATGGCGGCCGTTTTATTTCAACGTAATCAACGAAAAACCAGCTCAAGAGCTGGT‑TTTTTG‑TGTGGTG‑AAAAATTTCAAAATAAAAAATTTGT‑CGATTTTTCTAACAATTTTGTTTTCATTTTTg < 1:93730/139‑1 (MQ=255) aCCATTCATGTGAATGGCGGCCGTTTTATTTAAACGTAATCAACGAAAAACCAGCTCAAGAGCTGGT‑TTTTTG‑TGTGGTG‑TAAAATTTCAAAATAAAAAATTTTT‑CGATTTTTATAACAATTTTTTTTTGtttttttg > 6:246226/1‑136 (MQ=255) aCCATTCATGTGAATGGCGGCCGTGTTTTTTTAACGTAAACAAACAAAAAACACCGCAAGAGAGGGTGTTTTTG‑TTTGGGT‑AAAATTTAAAAAAAAAAAAATTTTG‑TGATTTTTTTTATAATTTTTTTTTAttttttt > 2:113702/1‑137 (MQ=255) aTTGATGTGAATGGCGGCCGTTTTATTTCAACGTAATCAACGAAAAACCAGCTCAAGAGCTGGT‑TTTTTG‑TGTGGTG‑AAAAATTTCAAAATAAAAAATTTGT‑CGATTTTTCTAACAATTTTGTTTTCATTTTTGCAAtt < 8:218339/139‑1 (MQ=255) aTTCATGTGAATGGCGGCCGTTTTATTTCAACGTAATCAACGAAAAACCAGCTCAAGAGCTGGT‑TTTTTG‑TGTGGTG‑AAAAATTTCAAAATAAAAAATTTGT‑CGATTTTTCTAACAATTTTGTTTTCATTTTTGCAAtt < 3:188081/139‑1 (MQ=255) aTTCATGTGAATGGCGGCCGTTTTATTTCAACGTAATCAACGAAAAACCAGCTCAAGAGCTGGT‑TTTTTG‑TGTGGTG‑AAAAATTTCAAAATAAAAAATTTGT‑CGATTTTTCTAACAATTTTGTTTTCATTTTTGCAAtt < 1:22617/139‑1 (MQ=255) aTTCATGTGAATGGCGGCCGTTTTATTTCAACGTAATCAACGAAAAACCAGCTCAAGAGCTGGT‑TTTTTG‑TGTGGTG‑AAAAATTTCAAAATAAAAAATTTGT‑CGATTTTTCTAACAATTTTGTTTTCATTTTTGCAAtt < 6:96073/139‑1 (MQ=255) aTTCATGTGAATGGCGGCCGTTTTATTTCAACGTAATCAACGAAAAACCAGCTCAAGAGCTGGT‑TTTTTG‑TGTGGTG‑AAAAATTTCAAAATAAAAAATTTGT‑CGATTTTTCTAACAATTTTGTTTTCATTTTTGCAAtt < 1:113702/139‑1 (MQ=255) ttCATGTGAATGGCGGCCGTTTTATTTCAACGTAATCAACGAAAAACCAGCTCAAGAGCTGGT‑TTTTTG‑TGTGGTG‑AAAAATTTCAAAATAAAAAATTTGT‑CGATTTTTCTAACAATTTTGTTTTCATTTTTGCAAttt < 3:54265/139‑1 (MQ=255) cATGTGAATGGCGGCCGTGTTTTTTAAAACTAATAAAACAAAAAACATCACAAGAGCAGGG‑TTTTTT‑TGTGGTT‑TAAAATTTCAAAAAAAAAAAATTGTGCAATTTTTTTAA‑AATTTAGTTTTTAATTTTTAAAtttt > 8:164/1‑138 (MQ=255) aTGTGAATGGCGGCCGTTTTATTTCAACGTAATCAACGAAAAACCAGCTCAAGAGCTGGT‑TTTTTG‑TGTTGTG‑AAAAATTTCAAAATAAAAAATTTGT‑CGATTTTTCTAACAATTTTGTTTTCATTTTTGCAATTTtat < 5:7780/139‑1 (MQ=255) aTGTGAATGGCGGCCGTTTTATTTCAACGTAATCAACGAAAAACCAGCTCAAGAGCTGGT‑TTTTTG‑TGTGGTG‑AAAAATTTCAAAATAAAAAATTTGT‑CGATTTTTCTAACAATTTTGTTTTCATTTTTGCAATTTtat < 2:37235/139‑1 (MQ=255) aTGTGAATGGCGGCCGTTTTATTTCAACGTAATCAACGAAAAACCAGCTCAAGAGCTGGT‑TTTTTG‑TGTGGTG‑AAAAATTTCAAAATAAAAAATTTGT‑CGATTTTTCTAACAATTTTGTTTTCATTTTTGCAATTTtat < 3:33731/139‑1 (MQ=255) tgAATGGCGGCCGTTTTATTTCAACGTAATCAAAGAAAAACCAGCTCAAGAGCTGGG‑TTTTTG‑TGTGGTG‑TTAAATTTAAAAATAAAAAATTTTT‑TGATTTTTATAAAAATTTTTTTTTGATTTTTTAAATTTtatatt > 3:225679/1‑138 (MQ=255) gAATGGCGGCCGTTTTATTTCAACGTAATCAACGAAAAACCAGCTCAAGAGCTGGT‑TTTTTG‑TGTGGTG‑AAAAATTTCAAAATAAAAAATTTGT‑CGATTTTTCTAACAATTTTGTTTTCATTTTTGCAATTTtatata < 3:264206/138‑1 (MQ=255) gcggcCGTTTTATTTCAACGTAATCAACGAAAAACCAGCTCAAGAGCTGGT‑TTTTTG‑TGTGGTG‑AAAAATTTCAAAATAAAAAATTTGT‑CGATTTTTCTAACAATTTTGTTTTCATTTTTGCAATTTTATATataata < 3:181493/138‑1 (MQ=255) ccGTTTTATTTCAACGTAATCAACGAAAAACCAGCTCAAGAGCTGGT‑TTTTTG‑TGTGGTG‑AAAAATTTCAAAATAAAAAATTTGT‑CGATTTTTCTAACAATTTTGTTTTCATTTTTGCAATTTTATATATAATATTGAg < 5:62474/139‑1 (MQ=255) gTTTTATTTCAACGTAATCAACGAAAAACCAGCTCAAGAGCTGGT‑TTTTTG‑TGTGGTG‑AAAAATTTCAAAATAAAAAATTTGT‑CGATTTTTCTAACAATTTTGTTTTCATTTTTGCAATTTTATATATAATATTGAGTa < 7:189432/139‑1 (MQ=255) gTTTTATTTCAACGTAATCAACGAAAAACCACCTCAAGAGCTGGT‑TTTTTG‑TGTGGTG‑AAAAATTTCAAAATAAAAAATTTGT‑CGATTTTTCTAACAATTTTGTTTTCATTTTTGCAATTTTATATATAATATTGAGTa < 7:193968/139‑1 (MQ=255) cGTAATCAACGAAAAACCAGCTCAAGAGCTGGT‑TTTTTG‑TGTGGTG‑AAAAATTTCAAAATAAAAAATTTGT‑CGATTTTTCTAACAATTTTGTTTTCATTTTTGCAATTTTATATATAATATTGAGTAGGAGAAAGGAAt > 3:156054/1‑139 (MQ=255) aTCAACGAAAAACCAGCTCAAGAGCTGGT‑TTTTTG‑TGTGGTG‑AAAAATTTCAAAATAAAAAATTTGT‑CGATTTTTCTAACAATTTTGTTTTCATTTTTGCAATTTTATATATAATATTGAGTAGGAGAAAGGAATGAtt > 8:103189/1‑139 (MQ=255) aaaaaCCAGCTCAAGCGCTGGT‑TTTTTG‑TGTGGTT‑AAAAATTTAAAAATAAAAATTTTGT‑CTATTTTTATAAAAATTTTTTTTTCATTTTTGAAATTTTATTTATATTTTTGAGTTGGATAAAGGATTGATTCGCGAcc < 8:174690/139‑1 (MQ=255) aaaaaCCAGCTCAAGAGCTGGT‑TTTTTG‑TGTGGTT‑AAAAATTTCAAAAAAAAAAATTTGT‑CGATTTTTCTAACAATTTTGTTTTAATTTTTGCAATTTTATATATAATATTGAGTAGGAGAAAGGAATGATTCGCGAcc < 4:103233/139‑1 (MQ=255) aaaaaCCAGCTCAAGAGCTGGT‑TTTTTG‑TGTGGTT‑AAAAATTTAAAAATAAAAAATTTGT‑CGATTTTTCTAACATTTTTGTTTTTATTTTGGCAATTTTATATTTAATATTGAGTAGTATAAAGTAATGATTCGCGAcc < 2:61631/139‑1 (MQ=255) aaaaaCCAGCTCAAGAGCTGGT‑TTTTTG‑TGTGGTG‑AAAAATTTCAAAATAAAAAATTTGT‑CGATTTTTCTAACAATTTTTTTTTCATTTTTGCAATTTTATATATAATATTGGGTAGGACAAAGGAATGATTCGCGAcc < 3:182144/139‑1 (MQ=255) aaaaaCCAGCTCAAGAGCTGGT‑TTTTTG‑TGTGGTG‑AAAAATTTCAAAATAAAAAATTTGT‑CGATTTTTCTAACAATTTTGTTTTCATTTTTGCAATTTTATATATAATATTGAGTAGGAGAAAGGAATGATTCGCGAcc < 4:156054/139‑1 (MQ=255) aaaaCCAGCTAACGAGCTGTT‑TTTTTG‑TGTGGTT‑AAAAATTTAAAAATAAAAAATTTGT‑GGATTTTTATAAAAATTTTTTTTTCATTTTTGAAATTTTATATATAATATTGAGTAGTATAAAGTAATTATTCGCGAc < 4:225679/137‑1 (MQ=255) ccAGCTCAAGAGATGGT‑TTTTTG‑TGTGGTT‑AAAAATTTCAAAATAAAAAATTTGT‑CGATTTTTATAACAATTTTGTTTTCATTTTTGAAATTTTATATATAATATTGAGTAGGATAAAGGAATGATTCGCGACCAGTAc < 8:150898/139‑1 (MQ=255) gCTCAAGAGCTGGT‑TTTTTG‑TGTGGTG‑AAAAATTTCAAAATAAAAAATTTGT‑CGATTTTTCTAACAATTTTGTTTTCATTTTTg < 3:176050/84‑1 (MQ=255) gCTCAAGAGCTGGT‑TTTTTG‑TGTGGTG‑AAAAATTTCAAAATAAAAAATTTGT‑CGATTTTTCTAACAATTTTGTTTTCATTTTTg > 4:176050/1‑84 (MQ=255) cTCAAGAGCTGGT‑TTTTTG‑TGTGGTG‑AAAAATTTCAAAATAAAAAATTTGT‑CGATTTTTCTTACAATTTTGTTTTGATTTTTGGAATTTTATATATAATATTGAGTAGGAGAAAGGAATGATTCGCGACCAGTACAAat > 3:398/1‑139 (MQ=255) ggT‑TTTTTG‑TGTGGTG‑AAAAATTTCAAAATAAAAAATTTGT‑CGATTTTTCTAACAATTTTGTTTTCATTTTTGCAATTTTATATATAATATTGAGTAGGAGAAAGGAATGATTCGCGACCAGTACAAATATAGTAGaa > 3:287252/1‑138 (MQ=255) ggT‑TTTTTG‑TGTGGTG‑AAAAATTTCAAAATAAAAAATTTGT‑CGATTTTTCTAACAATTTTGTTTTAATTTTTGCAATTTTATATATAATATTGATTAGGAGAAAGTAATGATTCGAGACCAGTACAAATATAGTATaa > 7:288476/1‑138 (MQ=255) tgtgGTG‑AAAAATTTAAAAATAAAAAATTTGT‑CGATTTTTATAACAATTTTGTTTTCATTTTTGCAATTTTATATATAATATTGAGTAGGAGAAAGGAATGATTCGCGACCAGTACAAATATAGTAGAAAAAAAAGaat < 7:61132/139‑1 (MQ=255) tggtg‑AAAAATTTCAAAATAAAAAATTTGT‑CGATTTTTCTAACAATTTTGTTTTCATTTTTGCAATTTTATATATAATATTGAGTAGGAGAAAGGAATGATTCGCGACCAGTACAAATATAGTAGAAAAAAAAGaataa > 8:41551/1‑139 (MQ=255) tggtg‑AAAAATTTCAAAATAAAAAATTTGT‑CGATTTTTCTAACAATTTTGTTTTCATTTTTGCAATTTTATATATAATATTGAGTAGGAGAAAGGAATGATTCGCGACCAGTACAAATATAGTAGAAAAAAAAGaata < 7:103189/138‑1 (MQ=255) aaaaaTTTAAAAATAAAAAATTTGT‑CGTTTTTTATAACATTTTTTTTTTCATTTTTGAAATTTTATATATAATATTGATTAGGAGAAAGGAATGATTCGCGACCAGTACAAATATAGTAGAAAAAAAAGAATAATGAc < 1:179315/138‑1 (MQ=255) aaaaaTTTAAAAAAAAAAAATTTGT‑CGATTTTTCTAACAATTTTGTTTTCATTTTTGAAATTTTAAATATAATATTGATTAGGCTAAAGTAATGATTCGCGACCAGTACAAATATAGTAGAAAAAAAAGAATAATGAc < 8:227034/138‑1 (MQ=255) | TGCTGGCGTCTGACGAATCTTCCTATATGACAGGGCAGACCATTCATGTGAATGGCGGCCGTTTTATTTCAACGTAATCAACGAAAAACCAGCTCAAGAGCTGGT‑TTTTTG‑TGTGGTG‑AAAAATTTCAAAATAAAAAATTTGT‑CGATTTTTCTAACAATTTTGTTTTCATTTTTGCAATTTTATATATAATATTGAGTAGGAGAAAGGAATGATTCGCGACCAGTACAAATATAGTAGAAAAAAAAGAATAATGAC > BS168_reference_genome/1023024‑1023279 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |