Predicted mutation
evidence seq id position mutation freq annotation gene description
RA BS168_reference_genome 1,224,521 A→G 100% E356G (GAA→GGA)  oppD → oligopeptide ABC transporter (ATP‑binding protein)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*BS168_reference_genome1,224,5210AG100.0% 187.6 / ‑6.6 53E356G (GAA→GGA) oppDoligopeptide ABC transporter (ATP‑binding protein)
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (26/26);  minor base T (0/1);  total (26/27)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TCCGATACTCATTATGTAAAATCGTGGCTGCTTCATCCTGACGCGCCAAAGGTAGAGCCGCCTGAAGCGGTAAAAGCGAAAATGCGTAAACTGGCAAACACGTTTGAAAAACCTGTCTTAGTGAGAGAAGTTGAATGAATTGACTGAAAAACTATTAGAAATCAAACATTTAAAACAGCACTTTGTCACGCCGAGGGGAACGGTTAAGGCTGTAGATGATTTATCATTTGATATCTATAAAGGTGAAACATTAGGGCTGGTTG  >  BS168_reference_genome/1224394‑1224656
                                                                                                                               |                                                                                                                                       
tCCGATACTCATTATGTAAAATCGTGGCTGCTTCATCCTGACGCGCCAAAGGTAGAGCCGCCTGAAGCGGTAAAAGCGAAAATGCGTAAACTGGCAAACACGTTTGAAAAACCTGTCTTAGTGAGAGGAGTtgaatgaa                                                                                                                              >  6:272806/1‑139 (MQ=255)
tCCGATACTCATTATGTAAAATCGTGGCTGCTTCATCCTGACGCGCCAAAGGTAGAGCCGCCTGAAGCGGTAAAAGCGAAAATGCGTAAACTGGCAAACACGTTTGAAAAACCTGTCTTAGTGAGAGGAGTtgaatgaa                                                                                                                              >  4:192216/1‑139 (MQ=255)
tCCGATACTCATTATGTAAAATCGTGGCTGCTTCATCCTGACGCGCCAAAGGTAGAGCCGCCTGAAGCGGTAAAAGCGAAAATGCGTAAACTGGCAAACACGTTTGAAAAACCTGTCTTAGTGAGAGGAGTtgaatgaa                                                                                                                              >  1:309635/1‑139 (MQ=255)
tCCGATACTCATTATGTAAAATCGTGGCTGCTTCATCCTGACGCGCCAAAGGTAGAGCCGCCTGAAGCGGTAAAAGCGAAAATGCGTAAACTGGCAAACACGTTTGAAAAACCTGTCTTAGTGAGAGGAGTtgaaggaa                                                                                                                              >  3:4554/1‑139 (MQ=255)
 ccGATACTCATTATGTAAAATCGTGGCTGCATCATCCTGACGCGCCAAACGTAGAGCCGCCTGAAGCCGGAAAAGCGAAAATGCGTAACCAGGCAAACACGTTTGAAAAACCTGTCTTAGTTAGAGGAGTAgaatgtat                                                                                                                             >  8:304565/1‑139 (MQ=255)
    aTACTCATTATGTAAAATCGTGGCTGCTTCATCCTGACGCGCCAAAGGTAGAGCCGCCTGAAGCGGTAAAAGCGAAAATGCGTAAACTGGCAAACACGTTTGAAAAACCTGTCTTAGTGAGAGGAGTTGAATGAATTGa                                                                                                                          <  4:108438/139‑1 (MQ=255)
        tCATTATGTAAAATCGTGGCTGCTTCATCCTGACGCGCCAAAGGTAGAGCCGCCTGAAGCGGTAAAAGCGAAAATGCGTAAACTGGCAAACACGTTTGAAAAACCTGTCTTAGTGAGAGGAGTTGAATGAATTGACTg                                                                                                                       >  4:302066/1‑138 (MQ=255)
          aTTATGTAAAATCGTGGCTGCTTCATCCTGACGCGCCAAAGGTAGAGCCGCCTGAAGCGGTAAAAGCGAAAATGCGTAAACTGGCAAACACGTTTGAAAAACCTGTCTTAGTGAGAGGAGTTGAATGAATTGACTGaa                                                                                                                     >  1:90250/1‑138 (MQ=255)
          aTTATGTAAAATCGTGGCTGCTTCAACCTGACGCGCCAAAGGTAGAGCCGCCTGAAGCGGTAAAAGCGAAAATTCGTAAACTGGCAAACACGTTTGAAAAACCTGTCTTAGTGAGATGAGTTGAATGAATTGACTGaa                                                                                                                     >  3:285020/1‑138 (MQ=255)
                    aTCGTGGCTGCTTCATCCTGACGCGCCAAAGGTAGAGCCGCCTGAAGCGGTAAAAGCGAAAATGCGTAAACTGGTAAACACGTTTGAAAAACCTGTCTTAGTGAGAGGAGTTGAATGAATTGACTGAAAAACTATTAg                                                                                                           >  4:127551/1‑138 (MQ=255)
                    aTCGTGGCTGCTTCATCCTGACGCGCCAAAGGTAGAGCCGCCTGAAGCGGTAAAAGCGAAAATGCGTAAACTGGCAAACACGTTTGAAAAACCTGTCTTAGTGAGAGGAGTTGAATGAATTGACTGAAAAACTATTAg                                                                                                           >  6:185448/1‑138 (MQ=255)
                        tGGCTGCTTCATCCTGACGCGCCAAAGGTAGAGCCGCCTGAAGCGGTAAAAGCGAAAATGCGTAAACTGGCAAACACGTTTGAAAAACCTGTCTTAGTGAGAGGAGTTGAATGAATTGACTGAAAAACTATTAGAAAt                                                                                                       >  1:276712/1‑138 (MQ=255)
                          gctgctTCATCCTGACGCGCCAAAGGTAGAGCCGCCTGAAGCGGTAAAAGCGAAAATGCGTAAACTGGCAAACACGTTTGAAAAACCTGTCTTAGTGAGAGGAGTTGAATGAATTGACTGAAAAACTATTAGAAATCaa                                                                                                    <  3:192216/139‑1 (MQ=255)
                          gctgctTCATCCTGACGCGCCAAAGGTAGAGCCGCCTGAAGCGGTAAAAGCGAAAATGCGTAAACTGGCAAACACGTTTGAAAAACCTGTCTTAGTGAGAGGAGTTGAATGAATTGACTGAAAAACTATTAGAAATCa                                                                                                     >  1:74475/1‑138 (MQ=255)
                          gctgctTCATCCTCACGCGCCAAAGGTAGAGCCGCCTGAAGCGGTAAAAGCGAAAATGCGTAAACTGGCAAACACGCTTGAAAAACCTGTCTTAGTGAGAGGAGTTGAATGAATTGACTGAAAAACTAATAGAAATCaa                                                                                                    <  7:220237/139‑1 (MQ=255)
                               gtCATCCTGACGCGCCAAAGGTAGAGCCGCCTGAAGCGGTAAAAGCGAAAATGCGTAAACTGGCAAACACGTTTGAAAAACCTGTCTTAGTGAGAGGAGTTGAATGAATTGACTGAAAAACTATAAGAAATCAAACAtt                                                                                               <  7:194336/138‑1 (MQ=255)
                                     cTGACGCGCCAAAGGTAGAGCCGCCTGAAGCGGTAAAAGCGAAAATGCGTAAACTGGCAAACACGTTTGAAAAACCTGTCTTAGTGAGAGGAGTTGAATGAATTGACTGAAAAACTATTAGAAATCAAACATTTAAAAc                                                                                         <  1:150794/139‑1 (MQ=255)
                                       gtcgcgCCAAAGGTAGAGCCGCCTGAAGCGGTAAAAGCGTAAATGCGTAAACTGGCAAACACGTTTGAAAAACCTGTCTTAGTGAGAGGAGTTGAATGAATTGACTGAAAAACTATTAGAAATCAAACATTTAAAACAg                                                                                       <  1:4685/137‑1 (MQ=255)
                                        aCGCGCCAAAGGTAGAGCCGCCTGAAGCGGTAAAAGCGAAAATGCGTAAACTGGCAAACACGTTTGAAAAACCTGTCTTAGTGAGAGGAGTTGAATGAATTGACTGAAAAACTATTAGAAATCAAACATTTAAAACAg                                                                                       >  1:14831/1‑138 (MQ=255)
                                         cgcgCCAAAGGTAGAGCCGCCTGAAGCGGTAAAAGCGAAAATGCGTAAACTGGCAAACACGTTTGAAAAACCTGTCTTAGTGAGAGGAGTTGAATGAATTGACTGAAAAACTATTAGAAATCAAACATTTAAAACAGCa                                                                                     <  2:102000/139‑1 (MQ=255)
                                             ccAAAGGTAGAGCCGCCTGAAGCGGTAAAAGCGAAAATGCGTAAACTGGCAAACACGTTTGAAAAACCTGTCTTAGTGAGAGGAGTTGATTGAATTGACTGAAAGACTATTAGAAAAGATACATTTAAAACAGCCCttt                                                                                 >  2:298131/1‑139 (MQ=255)
                                             ccAAAGGTAGAGCCGCCTGAAGCGGTAAAAGCGAAAATGCGTAAACTGGCAAACACGTTTGAAAAACCTGTCTTAGTGAGAGGAGTTGAATGAATTGACTGAAAAACTATTAGAAATCAAACATTTAAAACAGCACttt                                                                                 >  3:229100/1‑139 (MQ=255)
                                             ccAAAGGTAGAGCCGCCTGAAGCGGTAAAAGCGAAAATGCGTAAACTGGCAAACACGTTTGAAAAACCTGTCTTAGTGAGAGGAGTTGAATGAATTGACTGAAAAACTATTAGAAATCAAACATTTAAAACAGCACttt                                                                                 >  5:198126/1‑139 (MQ=255)
                                                      gagaCGCCTGAAGCCGTAAAAGCGAAAATGCGTAAACTGGCAAACACCTTTGAAAAACGTGTCTTATTGAGAGGAGTTGAATGAAATGACTGAAAAACTATTAGAAATCAAACATTTAAAACAGCACTTTGTCACGcc                                                                         >  5:76960/1‑138 (MQ=255)
                                                      gagCCGCCTGAAGCGGTAAAAGCGAAAATGCGTAAACTGGCAAACACGTTTGAAAAACCTGTCTTAGTGAGAGGAGTTGAATGAATTGACTGAAAAACTATTAGAAATCAAGCATTTAAAACAGCACTTTGTCACGcc                                                                         >  4:138051/1‑138 (MQ=255)
                                                      gagCCGCCTGAAGCGGTAAAAGCGAAAATGCGTAAACTGGCAAACACGTTTGAAAAACCTGTCTTAGTGAGAGGAGTTGAATGAATTGACTGAAAAACTATTAGAAATCAAACATTTAAAACAGCACTTTGTCACGcc                                                                         >  1:6399/1‑138 (MQ=255)
                                                            gcTGTAGCGGTATAAGAGAAAATGCGTAAACTGGCAAACACGTTTGAAAAACCTTTCTTAGTGAGTGGAGTTTAATGAATTGACTGAAAAACTATTATAAATCAAACATTTAAAACAGCACTTTGTCACGCCGAGGGGa                                                                  <  6:198126/138‑1 (MQ=255)
                                                            ccagAAGCGGTAAAAGCGAAATTGCGTAAACTGGGAAACACGGTTGAAAAACCTGTGTTTGTGAGTGTTGTTGAATGAATTGACTTAAAAACTATTAGAAATCAAACATTTAAAACAGCACTTTGTCACGCCGAGGGGa                                                                  <  4:285020/136‑1 (MQ=255)
                                                            ccTGAAGCGGTAAAAGCGAAAATGCGTAAACTGGCAAACACGTTTGAAAAACCTGTCTTAGTGAGAGGAGTtg                                                                                                                                    >  3:230095/1‑73 (MQ=255)
                                                            ccTGAAGCGGTAAAAGCGAAAATGCGTAAACTGGCAAACACGTTTGAAAAACCTGTCTTAGTGAGAGGAGTtg                                                                                                                                    <  4:230095/73‑1 (MQ=255)
                                                            ccTGAAGCGGTAAAAGCGAAAATGCGTAAACTGGCAAACACGTTTGAAAAACCTGTCTTAGTGAGAGGAGTTGAATGAATTGACTGAAAAACTATTAGAAATCAAGCATTTAAAACAGCACTTTGTCACGCCGAGGGGa                                                                  <  3:138051/139‑1 (MQ=255)
                                                            ccTGAAGCGGTAAAAGCGAAAATGCGTAAACTGGCAAACACGTTTGAAAAACCTGTCTTAGTGAGAGGAGTTGAATGAATTGACTGAAAAACTATTAGAAATCAAACATTTAAAACAGCa                                                                                     <  7:269969/120‑1 (MQ=255)
                                                            ccTGAAGCGGTAAAAGCGAAAATGCGTAAACTGGCAAACACGTTTGAAAAACCTGTCTTAGTGAGAGGAGTTGAATGAATTGACTGAAAAACTATTAGAAATCAAACATTTAAAACAGCa                                                                                     >  8:269969/1‑120 (MQ=255)
                                                            ccTGAAGCGGTAAAAGCGAAAATGCGTAAACTGGCAAACACGTTTGAAAAACCTGTCTTAGTGAGAGGAGTTGAATGAATTGACTGAAAAACTATTAGAAATCAAACATTTAAAACAGCACTTTGTCACGCCGAgggg                                                                   >  3:147722/1‑138 (MQ=255)
                                                            ccTGAAGCGGTAAAAGCGAAAATGCGTAAACTGGCAAACACGTTTGAAAAACCTGTCTTAGTGAGAGGAGTTGAATGAATTGACTGAAAAACTATTAGAAATCAAACATTTAAAACAGCACTTTGTCACGCCGAgggg                                                                   >  3:36902/1‑138 (MQ=255)
                                                            ccTGAAGCGGTAAAAGCGAAAATGCGTAAACTGGCAAACACGTTTGAAAAACCTGTCTTAGTGAGAGGAGTTGAATGAATTGACTGAAAAACTATTAGAAATCAAACATTTAAAACAGCACTTTGTCACGCCGAGGGGa                                                                  <  7:60441/139‑1 (MQ=255)
                                                            ccTGAAGCGGTAAAAGCGAAAATGCGTAAACTGGCAAACACGTTTGAAAAACCTGTCTTAGTGAGAGGAGTTGAATGAATTGACTGAAAAACTATTAGAAATCAAACATTTAAAACAGCACTTTGTCACGCCGAGGGGa                                                                  <  4:99691/139‑1 (MQ=255)
                                                            ccTGAAGCGGTAAAAGCGAAAATGCGTAAACTGGCAAACACGTTTGAAAAACCTGTCTTAGTGAGAGGAGTTGAATGAATTGACTGAAAAACTATTAGAAATCAAACATTTAAAACAGCACTTTGTCACGCCGAGGGGa                                                                  <  3:302066/139‑1 (MQ=255)
                                                            ccTGAAGCGGTAAAAGCGAAAATGCGTAAACTGGCAAACACGGTTGAAAAACCTGGCTTAGTGAGAGGAGTTGAATGAATTGACTGAAAAACTATTAGAAATCAAACATTTAAAACAGCCCTTTGTCACGCCGAGGGGa                                                                  <  7:195165/139‑1 (MQ=255)
                                                                     gTAAAAGCGAAAATGCGTAAACTGGCAAACACGTTTGAAAAACCTGTCTTAGTGAGAGGAGTTGAATGAATTGACTGAAAAACTATTAGAAATCAAACATTTAAAACAGTACTTTGTCACGCCGAGGGGa                                                                  <  2:225059/130‑1 (MQ=255)
                                                                     gTAAAAGCGAAAATGCGTAAACTGGCAAACACGTTTGAAAAACCTGTCTTAGTGAGAGGAGTTGAATGAATTGACTGAAAAACTATTAGAAATCAAACATTTAAAACAGTACTTTGTCACGCCGAGGGGa                                                                  >  1:225059/1‑130 (MQ=255)
                                                                     gTAAAAGCGAAAATGCGTAAACTGGCAAACACGTTTGAAAAACCTGTCTTAGTGAGAGGAGTTGAATGAATTGACTGAAAAACTATTAGAAATCAAACATTTAAAACAGCACTTTGTCACGCCGAGGGGAACGGTTAAg                                                         >  6:64580/1‑139 (MQ=255)
                                                                      tAAAAGCGAAAATGCGTAAACTGGCAAACACGTTTGAAAAACCTGTCTTAGTGAGAGGAGTTGAATGAATTGACTGAAAAACTATTAGAAATCAAACATTTAAAACAGCACTTTGTCACGCCGAGGGGAACGGTTAAgg                                                        <  5:185448/139‑1 (MQ=255)
                                                                            cGAATATGCGTAAACTGGCAAACACGTTTGAAAAACCTGTCTTAGTGTGAGGAGTTTATTGAATTGACTGATAAACTATTAGAAATCAAACATTTAAAACAGCACTTTGTCACGCCGAGTTGAACTGTTAAGGCTGTAg                                                  <  6:76960/139‑1 (MQ=255)
                                                                                     gTAAACTGCAAAACAGGTTTTAAAAACCTGTCTTTGTGACAGGTGTTTATTTAATTGACTTAAAAAAAATTATAAAACAAACATTTAAAACCGCACTTTGTCACGCCGAGGGGAACGGTTAAGGCTGTTGATGATTTAt                                         <  2:309635/139‑1 (MQ=255)
                                                                                         aCTGGCAAACACGTTTGAAAAACCTGTCTTAGTGAGAGGAGTTGAATGAATTGACTGAAAAACTATTAGAAATCAAACATTTAAAACAGCACTTTGTCACGCCGAGGGGAACGGTTAAGGCTGTAGATGATTTATCAtt                                     <  4:147722/139‑1 (MQ=255)
                                                                                             gtaaaCACGTTTGAAAAACCTGTCTTAGTGAGAGGAGTTGAATGAATTGACTGAAAAACTATTAGAAATCAAACATTTAAAACAGCACTTTGTCACGCCGAGGGGAACGGTTAAGGCTGTAGATGATTTATCATTTGat                                 <  3:127551/137‑1 (MQ=255)
                                                                                             gCAAACACGTTTGAAAAACCTGTCTTAGTGAGAGGAGTTGAATGAATTGACTGAAAAACTATTAGAAATCAAACATTTAAAACAGCACTTTGTCACGCCGAGGGGAACGGTTAAGGCTGTAGATGATTTATCATTTGat                                 <  5:64580/139‑1 (MQ=255)
                                                                                                           aaaaCCTGTCTTAGTGAGAGGAGTTGAATGAATTGACTGAAAAACTATTAGAAATCAAACATTTAAAACAGCACTTTGTCACGCCGAGGGGAACGGTTAAGGCTGTAGATGATTTATCATTTGATATCTATAAAGGTGa                   <  7:114039/139‑1 (MQ=255)
                                                                                                           aaaaCCTGTCTTAGTGAGAGGAGTCTTATGAATTGACTGAAATACTATTAGAAATCAAACATTTAAAACAGCTCTTTGTCAAGCCGAGGGGAACGGTTAAGGCTGTAGATGATTTATCATTTGATATCTATAAAGGTGa                   <  2:276712/139‑1 (MQ=255)
                                                                                                           aaaaCATGTCTTAGTGAGATGATTTGAATGAATTGACTGAAAAACTATTAGAAATCAAACATTTAAAACAGCACTTTGTCACGCCGAGGGGAACGGTTAAGGCTGTAGATGATTTATCATTTGATATCTATAAAGGTGa                   <  2:90250/139‑1 (MQ=255)
                                                                                                               ccTGTCTTAGTGAGAGGAGTTGAATGAATTGACTGAAAAACTATTAGAAATCAAACATTTAAAACAGCACTTTGTCACGCCGAGGGGAACGGTTaa                                                          >  3:253575/1‑96 (MQ=255)
                                                                                                               ccTGTCTTAGTGAGAGGAGTTGAATGAATTGACTGAAAAACTATTAGAAATCAAACATTTAAAACAGCACTTTGTCACGCCGAGGGGAACGGTTaa                                                          <  4:253575/96‑1 (MQ=255)
                                                                                                                            gaggagTTGAATGAATTGACTGAAAAACTATTAGAAATCAAACATTTAAAACAGCACTTTGTCACGCCGAGGGGAACGGTTAAGGCTGTAGATGATTTATCATTTGATATCTATAAAGGTGAAACATTAGGGCtggttg  <  2:14831/139‑1 (MQ=255)
                                                                                                                               |                                                                                                                                       
TCCGATACTCATTATGTAAAATCGTGGCTGCTTCATCCTGACGCGCCAAAGGTAGAGCCGCCTGAAGCGGTAAAAGCGAAAATGCGTAAACTGGCAAACACGTTTGAAAAACCTGTCTTAGTGAGAGAAGTTGAATGAATTGACTGAAAAACTATTAGAAATCAAACATTTAAAACAGCACTTTGTCACGCCGAGGGGAACGGTTAAGGCTGTAGATGATTTATCATTTGATATCTATAAAGGTGAAACATTAGGGCTGGTTG  >  BS168_reference_genome/1224394‑1224656

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑